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Egg production, larval development, and adult longevity of cat fleas (Siphonaptera: Pulicidae) exposed to ultrasound.

Adult cat fleas, Ctenocephalides felis felis (Bouché), on cats (Felis catus) were exposed to emissions from an ultrasonic flea collar worn by the cat. No significant differences were found in total numbers of eggs produced per day (mean = 524 control, 614 treatment), in length of larval development time (mean = 7.7 d control, 7.7 d treatment), or in total daily pupal production (mean = 485 control, 445 treatment) between the treatment and the control groups. Tests off the host were conducted to determine whether ultrasonic exposure caused mortality in adult fleas; no significant differences were found in daily mortality between the treated and control fleas during 1 wk of exposure.

Animals↗

Separation of cat flea (Siphonaptera: Pulicidae) instars by individual rearing and head width measurements.

Two methods to verify whether head width measurements fit Dyar's rule were evaluated for the separation of instars of the cat flea, Ctenocephalides felis (Bouché). Individual rearing was a reliable method of determining larval instar but was labor-intensive. The mean observed head widths were significantly different for each instar (first instar, 0.164 mm; second instar, 0.201 mm; third instar, 0.260 mm) and showed no sexual dimorphism. Head capsule width increased roughly 25% from instar to instar with geometrically progressing growth in accordance with Dyar's rule. However, head capsule width cannot be used to determine the instar of randomly selected larvae because the measurements overlap broadly between instars.

Animals↗

Effect of larval diet on cat flea (Siphonaptera: Pulicidae) developmental times and adult emergence.

The natural diet of cat flea, Ctenocephalides felis (Bouche), larvae is primarily adult flea feces, but dried bovine blood may be substituted in the laboratory. Percentage adult emergence (79.4% on feces; 78.9% on blood) and developmental times (20.6 d on feces; 17.1 d on blood) did not significantly differ for the two diets. The drying temperature of blood determined its quality; blood dried at 120 degrees C was unsatisfactory for larval development. The dietary value of dried bovine blood was not enhanced when supplemented with brewer's yeast, rodent chow, or a combination of those constituents. Blood particle size ranging from less than 180 to greater than 500u did not affect the value of blood as a diet. Rodent chow, yeast, albumen, hemoglobin, and mixtures of these constituents were unsuitable as larval diets.

Animals↗

Effect of methoprene and diflubenzuron on larval development of the cat flea (Siphonaptera: Pulicidae).

Cat flea larvae, Ctenocephalides felis Bouche, exposed to glass surfaces treated with methoprene concentrations from 0.127 to 1,270 ng/cm2 did not emerge as adults. Most larvae died in the third instar, but those exposed to the 0.127 ng/cm2 concentration formed larval-pupal intermediates. Larvae exposed to glass surface treated with diflubenzuron concentrations from 12.7 to 1,270 ng/cm2 died during the process of molting in all three instars. Exposure of larvae to 12.7 and 127 ng/cm2 diflubenzuron resulted in 15 and 5.2% adult emergence, respectively.

Animals↗

Oral toxicity of boric acid and other boron compounds to immature cat fleas (Siphonaptera: Pulicidae).

Oral toxicity was characterized in first-instar cat fleas, Ctenocephalides felis felis (Bouché), feeding on dried blood treated with boric acid. LC50 values ranged from 2.11% after 24 h to 0.21% after 7 d. In carpet tests with five different boron compounds and a number of different formulations, significant mortality for first instars was observed in all cases. In similar tests with prepupae and cocoons, there was no significant effect on mortality. The importance of these results is discussed in light of current application procedures for boron compounds, and suggestions are made for future research.

Administration, Oral↗

Residual effectiveness of insect growth regulators applied to carpet for control of cat flea (Siphonaptera: Pulicidae) larvae.

Three insect growth regulators, fenoxycarb, methoprene, and pyriproxyfen, formulated as total release aerosols, were tested for their residual effectiveness on carpet in bioassays with cat flea, Ctenocephalides felis (Bouché), larvae. All treatments except methoprene produced significant mortality for the 7-mo duration of the test. In general, fenoxycarb and the higher rates of pyriproxyfen reduced adult flea emergence by > 80%.

Animals↗

Chlorpyrifos formulation effect on airborne residues following broadcast application for cat flea (Siphonaptera: Pulicidae) control.

Airborne residues of 3 chlorpyrifos formulations were measured up to 50 h after broadcast treatment for flea control in residences. Insecticide formulation, time after treatment, ventilation regime, and height above floor affected airborne residues. Before spraying, chlorpyrifos residues were low or undetected. In nonventilated residences, chlorpyrifos residues peaked in 0-6 h after treatment at 38 ng/liter for emulsifiable, remained low at < 12 ng/liter for the microencapsulated, and sharply peaked in 1-2 h after treatment at 52 ng/liter for the aerosol formulation. Residues for all formulations then slowly declined through 50 h. In ventilated residences, chlorpyrifos residues peaked from 2 to 10 h after treatment at 25-27 ng/liter for emulsifiable, remained low at < 10 ng/liter, and sharply peaked in 0-2 h after treatment at 21 ng/liter for the aerosol formulation. Maximum airborne concentrations were 74 ng/liter for emulsifiable, 17 ng/liter for microencapsulated, and 61 ng/liter for aerosol chlorpyrifos.

Air Pollutants↗

Airborne insecticide residues after broadcast application for cat flea (Siphonaptera: Pulicidae) control.

Airborne residues of chlorpyrifos, propetamphos, and permethrin were measured up to 50 h after broadcast treatment for cat flea, Ctenocephalides felis (Bouché), control in residences. Type of insecticide, time after treatment, ventilation regime, and height above floor significantly affected airborne residues. Before spraying chlorpyrifos, residues were low or undetected. In nonventilated residences, chlorpyrifos residues peaked 0-6 h after treatment at 38 ng/liter, then slowly declined through 50 h. In ventilated residences, chlorpyrifos residues peaked from 2 to 10 h after treatment at 25-27 ng/liter, then declined to 8 ng/liter at 50 h. Propetamphos and permethrin residues in nonventilated residences peaked at 0-2 h at 32-40 and 40-44 ng/liter, respectively. Propetamphos peaked at 12-17 ng/liter at 0-2 h in ventilated residences. Permethrin was not detected in any of the samples in ventilated residences. Maximum airborne concentrations were 74 ng/liter chlorpyrifos, 49 ng/liter propetamphos, and 54 ng/liter permethrin.

Air↗

Insecticide susceptibility of cat flea (Siphonaptera: Pulicidae) pupae.

Studies were conducted to investigate what protective nature the cocoon and nylon carpeting has against the performance of insecticides directed at cat flea, Ctenocephalides felis (Bouché), pupae developing in carpet. The following 5 combinations of life stages and substrates were used in these tests were: (1) larvae that pupated in carpet, (2) cocoons placed in carpet, (3) naked pupae placed in carpet, (4) cocoons placed on filter paper, and (5) naked pupae placed on filter paper. These studies evaluated the performance of chlorpyrifos, microencapsulated chlorpyrifos, propetamphos, permethrin, and linalool. When averaged over all insecticides, the mean controls of cocooned pupae placed in carpet and cocooned pupae placed on filter paper were 37.2 and 26.7%, respectively, whereas that of naked pupae placed in carpet was only 13.7%. Additional tests conducted using chlorpyrifos revealed that mortality was 23 and 42% higher, respectively, whenever pupae in cocoons and naked pupae were treated in glass dishes without filter paper. These studies demonstrate that the debri-coated cocoon is not a barrier to insecticide penetration, and that pupae appeared to be protected inside the carpet matrix. Additional studies demonstrated that control of pupae developing in carpet was maximized at lower application pressures. The mean control of pupae with chlorpyrifos at 0.7 kg/cm2 pressure was 77.2%, yet applications at 1.7 kg/cm2 resulted in only 23.3% control.

Animals↗

Activity of insecticides applied to turfgrass to control adult cat fleas (Siphonaptera:Pulicidae).

A bioassay was developed to determine the efficacy of insecticides against adult cat fleas, Ctenocephalides felis (Bouché), on turfgrass. Activity was assessed by exposing adult fleas to treated and untreated disks of turf for 24 h, 1 and 7 d after treatment. Filter paper, a representative nonwaxy, porous substrate was bioassayed in similar fashion 1 d after treatment to confirm insecticide activity. Results demonstrated the relative efficacy of insecticides applied to turf at different rates as well as differences among insecticides. Of 10 insecticides tested, chlorpyrifos wettable powder and emulsifiable concentrates consistently provided greatest kill of adult fleas. Although initially active, pyrethroid insecticides and diazinon generally showed significant declines in residual activity by day 7. Possible reasons for differences in activity are discussed.

Animals↗

Effect of temperature and the synergist piperonyl butoxide on imidacloprid toxicity to the cat flea (Siphonaptera: Pulicidae).

The toxicity of imidacloprid to cat fleas on glass was investigated at 20, 26, 30, and 35 degrees C. Imidacloprid was most toxic to adult cat fleas at 35 degrees C and to larvae at 20 degrees C. Piperonyl butoxide (PBO), a synergist, increased the relative potency of imidacloprid (1:5 imidacloprid:PBO) 16-fold at 26 degrees C against adults, but had no effect at 35 degrees C. No synergism occurred in larvae at 20 degrees C, but addition of PBO (1:5 imidacloprid:PBO) doubled toxicity at 26 degrees C. PBO (1:5 imidacloprid:PBO) could possibly be used to synergize imidacloprid premise treatments (20-30 degrees C), but it is not likely to be effective in pet treatments because no synergism occurred in adult fleas at 35 degrees C (average fur temperature of tested cats and dogs).

Animals↗