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PubMed1980

[Cell proliferation kinetics in sarcoma 37. I. The changes in the size of the cell and the nucleus over the generative cycle].

Using morhometric autoradiographic and cytophotometric methods, the author examined the changes in the size of the nucleus, and of the cell as well as of the ratio: surface of the nucleus (surface of the cytoplasma in sarcoma-37 ascitic variant in accordance with their position in the generative cycle. The cells with small sizes of the nucleus 30 microns 2 were not labelled with 3H-thymidine and were found mainly in Gi-phase of the cycle. The labelled cells with nuclear sizes of 40-60 microns 2 were at the S-phase of the cycle. The labelled cells with larger sizes of the nucleus either were leaving S and entered Gi phase of the cycle or there was a subpopulation of cells with an increased number of chromosomes. The data on the presence of nonlabelled nuclei and cells with equal cells with equal sizes of those of the labelled cells as well as the elevated values of DNA, exceeding the tetraploid region of the hystogram, suggested heterogenesis of the cellular population in sarcoma-37.

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