[Regulation of reproductive medicine in North America or the Wild West of Medicine (Part II)].
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In vitro fertilization (IVF) is now an accepted treatment procedure for subfertility. Its use has also contributed greatly to the understanding of our knowledge in reproductive medicine. This review looks at these contributions. In the female, IVF has made it possible to examine more closely ovarian physiology through the follicular micro-environment, follicular dominance and cohorts. In the male, the new sperm preparation technology has assisted the investigation of sperm parameters in-depth. A better understanding of human egg and sperm physiology, the fertilization process and pre-implantation phenomena has also contributed to better management of the infertile couple. Perhaps in the future, the pre-implantation embryo may be considered as a "patient".
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Since the first child was born after in-vitro fertilization in 1978, different techniques of ovarian stimulation, oocyte retrieval and embryo culture have improved significantly. The development of microinjection techniques was a mile stone in reproductive medicine. Even in cases of severe impairment of semen quality, using the Intracytoplasmic Sperm Injection (ICSI), pregnancy rates of approximately 20 % per treatment cycle can be achieved. However for using artificial reproductive techniques (ART) the female age is a major limiting factor as well as the development of premature ovarian failure (POF). Crucial for the male is the question whether or not sperm cells can be retrieved. Today an interdisciplinary approach is recommended to take care of the afflicted couple in order to address the complex problem of infertility. There is evidence that children born after microinjection techniques bare a higher risk for chromosomal aberrations compared to the normal population. However rates of major malformations seem to be identical in both groups. An evaluation of the potential risks and efforts and an extensive counseling of the infertile couple has to be taken before initiating invasive ART.
The human ovary is a dynamic organ which continually changes in size and activity through life, as an integral part of the changes that the female is going through before during and after her reproductive life. Following the rapid increase in the use of transvaginal scan in recent years, the measurement of ovarian volume has become quick, accurate and cost-effective. Ovarian volume is an important tool in the screening, diagnosis and monitoring the treatment of conditions such as polycystic ovarian syndrome, ovarian cancer and adolescent abnormalities. In reproductive medicine, measurement of ovarian volume has a role in the assessment of ovarian reserve and prediction of response to superovulation.
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Adoption and medically assisted reproduction are alternative options for childless parents to fulfill their wish for having a child. In both situations, there is a necessity for the future parents to mourn the loss of their imagined child, which could have been conceived without a third party (adoption agency or medically assisted reproduction). Adoption always represents for the child a loss of emotional ties with birthparents and the development of new attachments with adoptive parents. Adoption can be considered as a lifetime process of the members involved in the adoption triangle, that is birth parents, adoptive parents and the child. The article discusses the loss of emotional bonds from primary caretakers as a psychological trauma and addresses mourning difficulties in adoptees. Problems with the development of new attachments with adoptive parents such as loyalty conflicts and the revelation of the adoption are described. Family romance fantasy in adoption and aspects of family dynamics as well as the specific difficulties adoptive parents encounter are explored.
Major advances in assisted reproductive technology and fertility enhancement in the last decade are discussed in this article. Sustained research efforts have led to refinement in in vitro fertilization and related technologies. Other therapeutic innovations such as the use of newer pharmacologic agents and surgical procedures to preserve reproduction function are highlighted. Future directions and anticipated developments are briefly reviewed.
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The birth of the first baby following in-vitro fertilisation and embryo transfer (IVF/ET) in 1978 and the introduction of gamete intrafallopian transfer (GIFT) in 1984 have increased the treatment modalities in operative reproductive medicine. In tubal pathology, there are besides micro-surgery now so-called additive methods available for treating infertility. With regard to the indications, there has been severe confusion, and it is therefore imperative to define special indications for IVF/ET, microsurgery and GIFT. We do not consider these treatment modalities as alternative methods. Reproductive centres should offer all these treatments to guarantee an appropriate individual programme for each couple. Therefore, knowledge of pregnancy rates is a prerequisite for the doctor. The aim of this paper is, to define the optimal therapy while taking into account the individual problems of each couple.
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Each year more veterinarians are seeing ornamental fish patients and our knowledge of how to manage the pet fish patient is increasing at a rapid pace. A large percentage of these patients are nishikigoi (Cyprinus carpio) which are taxonomically an ornamental carp. In this country and in many parts of the world we refer to these fish as koi. Reproductive medicine problems are well documented in koi and a thorough knowledge of this subject will aid the fish practitioner in diagnosis and treatment.
Even though infertility seems to be an old phenomenon, it will be argued that it was discovered in a new way once it became necessary to legitimize the technological possibilities of artificial fertilization at the end of the 1970s. With the birth of the first test-tube baby in 1978 the public became excited about the new possibilities of medical procreation. Reactions were extremely divided because extracorporeal fertilization seemed to undo the difference between natural organisms and technologically created human beings. Today reproductive medicine is an accepted field of gynecology and an increasing number of couples make use of it (even in managing their fertility). This development was the result of the successful integration of reproductive techniques into the discourse of infertility which is by no means self-evident. From the perspective of how societies communicate about reproduction it becomes clear that the dividing line between fertility and infertility is disappearing.
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The "New Genetic Era" will be a period of enormous exponential growth in our knowledge of the structure and function of the basic information blocks of life. The Human Genome Project will soon provide a complete and accurate sequence of the human genome. This will give us an abundance of basic genetic knowledge and provide a molecular understanding of disease, allowing for improved diagnosis and more sensitive and specific screening for disease. This will, we hope, lead to better treatments, prevention and cures through gene therapy, patient-specific drug design, and earlier and more specific behavioral interventions to prevent disease. With this information comes a complexity of legal, ethical and social concerns about potential use and abuse. The public has expressed its concerns about the potential for genetic discrimination. However, genetic information is enhancing our knowledge as to the causes of infertility, allowing diagnosis of more diseases in the prenatal period, and may aid our identification of patients at increased risk for breast and ovarian cancer. Doctors involved in reproductive medicine must become knowledgeable about the new genetic era so as to offer our patients the most appropriate and informed care.
A total of 30 ejaculates has been tested for ejaculate volume, spermatozoal concentration, motility, agglomeration, agglutination and contamination by microorganisms. After that the ejaculate has been washed twice and transferred in the Dr. Wangs Tube which was filled with medium before. After an incubation time of 90 minutes very quickly moving spermatozoan could be observed through the microscope in the upper part (C-D) of the Tube. After the Tube was cut by means of a special glasscutter the motility of the containing spermatozoans of part C-D has been determined again, followed by a second bacteriological examination of the so prepared ejaculate. The results of sperm preparation with the Dr. Wangs Tube regarding the sperm motility has been very good (85.7% of the sperm samples showed a motility rate of 90% or even more after treatment). The bacteriological examination has been satisfactory: in 77.7% of the cases all existing sperm bacterias has been eliminated by preparation in the Dr. Wangs Tube. Among other microorganisms Escherichia coli, Proteus mirabilis and Staphylococcus aureus which are considered to be pathologic could be isolated from five of the ejaculates and have been removed after treatment with the Dr. Wangs Tube. The handling of the Tube was rather demanding and Technically problematic. There has been caused glass-splinters by cutting the Tube which got visible while observing the ejaculate-medium-liquid through the microscope. Their sizes were nearly equivalent to the size of a sperms head or even bigger. If the so prepared ejaculate would be used for insemination and in-vitro fertilisation the very small splinters may destroy the success of the treatment and should be avoided or removed. The Dr. Wangs Tube offers a new modified swim-up technique for sperm preparation and is able to produce a high quality, nearly sterile sperm sample. Because of the technical deficiency of the system, the high purchase and running costs and the very small output (sperm sample volume, number of spermatozoans) the Dr. Wangs Tube is not be recommended for the reproductive medicine in its present shape.
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