Search PubMed⌕ Search

SEARCH · Search PubMed

Results for “Reporter virus”

Search indexed PubMed citations on genomics, clinical trials, systematic reviews and public health. Explore titles, authors and supplied subject terms, then open the PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 109 records · Page 6Linked to original sources
PubMed2002

Andes virus and first case report of Bermejo virus causing fatal pulmonary syndrome.

Two suspected hantavirus pulmonary syndrome (HPS) cases from Bolivia occurred in May and July 2000 and were confirmed by enzyme-linked immunosorbent assay (ELISA)-ANDES using N-Andes recombinant antigen serology. Clot RNAs from the two patients were subjected to reverse transcription-polymerase chain reaction (PCR) amplification and sequencing. We describe two characterized cases of HPS. One was caused by infection with Bermejo virus and the other with Andes Nort viral lineage, both previously obtained from Oligoryzomys species. This is the first report of molecular identification of a human hantavirus associated with Bermejo virus.

Adult↗
PubMed1980

Detection of C-type virus by immunoferritin technique in bat lung cell line chronically infected with bovine leucosis virus.

Reported in this paper are morphological studies and tests for the detection of Type-C particles from a line of bat lung cells chronically infected with bovine leucosis virus. The immunoferritin technique was used. Ferritin labelling of Type-C particles was regularly accompanied by black-spot arrangement of ferritin around the virus envelope, which provided evidence to the specificity of this immunochemical technique.

Animals↗
PubMed2004

Punctate exanthem of West Nile Virus infection: report of 3 cases.

The West Nile Virus (WNV) has rapidly emerged as an important etiology of meningoencephalitis in North America since 1999. Diagnosis of this infection on clinical grounds is difficult, as many signs and symptoms of infection are nonspecific. Although cutaneous manifestations are common in WNV-infected patients, these have not been described in detail nor are clinical images widely available. We describe 3 patients with WNV infections, two ambulatory, one hospitalized, who developed punctate erythematous, macular, and papular eruptions, most pronounced on the extremities. Histopathologic findings in one case showed a sparse superficial perivascular lymphocytic infiltrate, a feature commonly seen in viral exanthems but not previously reported with WNV infection. A literature review provides support that this punctate exanthem is a common cutaneous presentation of WNV infection.

Adult↗
PubMed2003

UNA virus: first report of human infection in Argentina.

Una virus (UNAV), Togaviridae family, is widely distributed in South America, where infections have been detected in mosquitoes and vertebrate hosts (humans, birds and horses). We analyzed human sera from Córdoba inhabitants aged 44 to 89 years and using a neutralization test, we found a prevalence of UNAV antibodies of 3.8% (3/79). The low titers detected suggest past infections probably acquired in rural areas of the Province of Córdoba (central Argentina). None sera were found positive for MAYV neutralizing antibodies. This is the first report of human infections by UNAV in Argentina.

Adult↗
PubMed1987

Genome isomerism in two alphaherpesviruses: Herpesvirus saimiri-1 (Herpesvirus tamarinus) and avian infectious laryngotracheitis virus. Brief report.

Genome isomerism of avian infectious laryngotracheitis virus (ILT) and Herpesvirus saimiri-1 (HVS-1) (formerly H. tamarinus) was investigated using restriction endonuclease analysis and scanning densitometry. ILT DNA was found to have 2 isomers with a molecular weight of 109 X 10(6). HVS-1 DNA was found to have four isomers with a molecular weight of 102 X 10(6).

Animals↗
PubMed2001

[Acute cerebellitis caused by Epstein-Barr virus: case report].

Acute cerebellitis can occur in association with varicella-zoster virus, enterovirus, mumps, mycoplasma, and other infective organisms. Acute cerebellitis is a rare complication of Epstein-Barr virus (EBV) infection. We report the case of a 21-year-old woman with a 12-day history of nausea and vomiting, gait and limbs ataxia, myoclonus, tremor of head and all four limbs, opsoclonus and cutaneous rash. Anti-EBV IgG and IgM antibodies against antiviral capsid were positive and anti-EBV against virus-associated nuclear antigen was also positive. EBV infection in association with neurological findings can occur without the classic signs and symptoms of infectious mononucleosis.

Acute Disease↗
PubMed2005

A novel small reporter gene and HIV-1 fitness assay.

Most currently available HIV-1 reporter gene constructs are large and disrupt the nef reading frame. This report describes a novel reporter gene based on the small murine heat stable antigen (HSA) protein, which is expressed on the surface of infected cells. This HSA reporter can be inserted in the vpr reading frame, leaving nef intact. Nine amino acids from the extracellular domain of HSA are replaced with an influenza hemagglutinin (HA) antibody epitope (HSA-HA). Like the parental reporter protein, this novel reporter is expressed on the surface of infected cells. Antibodies for HSA and HA specifically detect reporter viruses with each construct, indicating disruption of the original HSA antibody epitope. Finally, a strategy is developed to detect each reporter virus by real-time PCR quantitation. The growth of viruses tagged with each reporter allows precise assessment of the relative growth of viruses differing in mutations of interest. Moreover, the availability of these reporters in either of two half-genome plasmids allows convenient production of reporter and non-reporter HIV-1 by co-transfection of appropriately paired plasmids. These paired reporter viruses offer a potentially useful standardized method for measurement of HIV-1 fitness in competition assays.

Alanine↗
PubMed1991

Haemophagocytosis in dengue haemorrhagic fever: a case report.

Virus associated haemophagocytic syndrome (VAHS), a distinct clinico-pathologic entity, is characterised by systemic proliferation of non-neoplastic histotiocytes showing haemophagocytosis resulting in blood cytopaenia. It has been described in relation to several viruses earlier. Here we describe a young girl who developed this reactive process during the course of dengue haemorrhagic fever (DHF).

Adult↗
PubMed2003

West Nile virus: case report with MR imaging findings.

We report a case of serologically proved West Nile virus encephalitis. Striking bilateral hyperintensities were noted in the basal ganglia and thalami on T2-weighted and fluid-attenuated inversion recovery MR images, similar to previous accounts of the imaging findings in cases of Japanese encephalitis. Recognition of the MR imaging appearance of this entity is important because of the expanding epidemic.

Female↗
PubMed1985

Isolation of large and small plaque variants of the Autographa californica nuclear polyhedrosis virus. Brief report.

Variants of the Autographa californica nuclear polyhedrosis virus which produce large (1.4 mm) or small (0.5 mm) plaques on Spodoptera littoralis cells have been isolated. Yields of extracellular virus and polyhedra by the large plaque variant were six-fold higher and 140-fold lower, respectively, than those obtained with the small plaque variant. However the two variants could not be distinguished when Spodoptera frugiperda cells were used.

Animals↗
PubMed1979

Cell fusion induced by invertebrate virus. Brief report.

Inoculation of invertebrate cells with Chilo Iridescent Virus (CIV) leads rapidly to massive formation of syncytia. This polykaryocytosis occurs at a high multiplicity of infection and does not require viral genome expression. Thus, cell fusion appears in non permissive conditions, and CIV suspensions rendered non infections by UV irradiation still retain the ability to produce rapid and extensive cell fusion. In the same way, some polykaryocytes could be detected in vertebrate cells (CV1 cell line). However, this process is more effective and faster in invertebrate cell systems.

Aedes↗