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PubMed2006

Rejuvenation by shoot apex culture recapitulates the developmental increase of methylation at the maize gene Pl-Blotched.

Cytosine methylation provides an attractive epigenetic modification for the global maintenance of phases in plant development; however, there are few known examples of specific genes whose methylation status changes in a developmentally regulated manner. Pl-Blotched, an allele of purple plant1 (pl1), which encodes a myb-like transcription factor that regulates anthocyanin production in maize, is one such gene: certain cytosines at the 3' end of this allele are hypomethylated in seedlings, become hypermethylated in organs formed in the adult phase, and are hypomethylated again in the next generation. We tested whether this developmental pattern of low juvenile cytosine methylation followed by higher methylation in adult tissues could also be observed in plants "rejuvenated" via shoot apex culture. We found that cytosine methylation patterns at Pl-Blotched were indeed recapitulated in culture-rejuvenated plants, showing hypomethylation in leaves with juvenile patterns of differentiation (even though they were made by an old meristem) followed by hypermethylation in later-formed leaves. Our results show that methylation status at that locus is determined by the developmental phase of the shoot, rather than by the age of the meristem forming it. These results support the hypothesis that DNA methylation is employed by the plant to maintain an epigenetic state.

Cytosine↗
PubMed2004

Use of a nonablative radiofrequency device to rejuvenate the upper one-third of the face.

OBJECTIVE: To evaluate the use of the ThermaCool TC nonablative radiofrequency device for rejuvenation of the upper one-third of the face, as determined by brow elevation. Study design and setting Twenty-four patients in a facial plastic surgery office were treated with the nonablative radiofrequency device. The patients had pretreatment photos, in-office procedure, and follow-up photos. Brow elevation measurements were used to gauge efficacy of the procedure. These results were compared to an untreated, control group of 12 patients. RESULTS: Compared to the control group, the post-treatment measurements were improved (P < 0.05). The post-treatment measurements were also improved from pretreatment baseline (P < 0.05). Subjective results obtained from patient satisfaction questionnaires did not correlate to the objective data. The data also showed that improvement in brow elevation was not uniform in each patient. CONCLUSION: The ThermaCool TC nonablative radiofrequency device for in-office rejuvenation of the upper one-third of the face procedure does provide brow elevation in a majority of patients; however, the majority of the study patients did not perceive benefit from the procedure. Patient satisfaction in facial plastic surgery must be an important part of the decision of whether or not to introduce a new device into a practice setting.

Adult↗
PubMed2004

Hyaluronic acid and its use as a "rejuvenation" agent in cosmetic dermatology.

Since 1996, hyaluronic acid (HA) has been launched onto the market in Europe. Since then, different companies proposed their HAs. Biomatrix (NJ, USA) proposes an animal-derived HA (from rooster comb). Q-Med AB (Uppsala, Sweden) and LEA-DERM (Paris, France) are the main companies to have a nonanimal HA. HA is produced by bacterial fermentation from a specific strain of streptococci. HA has no species specificity and theoretically has no risk of allergy. No skin testing is necessary before injecting because HA is a biodegradable agent. To be utilized as a filler agent for improving wrinkles, scars, or increasing volumes, HA must be stabilized to obtain a sufficient half-life. Process of stabilization varies, according to each manufacturer. This explains the differences in longevity and in viscosity of the different products. Several HAs are suitable to fine lines, to deep wrinkles/folds, or to increase volume. A new indication for "rejuvenation" is injection into the superficial dermis and epidermis. The HA (stabilized or not) is not used to fill in but rather to hydrate and finally to rejuvenate the skin. This procedure must be repeated at intervals of a few weeks or months. If HA is the safest filler agent in cosmetic indications today, some rare side effects may appear and must be known to inform patients. Most of these complications are not severe and will disappear when the product is degraded.

Biocompatible Materials↗
PubMed2002

Matrix-mediated cellular rejuvenation.

Biomaterial surface morphology and chemistry influence cell responses mediated via signaling cascades that regulate a wide range of metabolic processes. These responses may range from changes in surface adhesion and remodeling of the extracellular matrix to activation of cytokine, cytoskeletal and other biochemical pathways regulating or modulating cellular morphology and function. The present study has focused on collagen Type I, a key extracellular matrix protein, and its potential impact on the process of cellular aging. This study was undertaken for several reasons. First, several investigators reported that growth of cells on a collagen matrix markedly enhanced the resistance of cells to stresses. Second, a large body of accumulated data strongly indicated a relationship between the potential to respond to stresses and cellular aging with the former strongly influencing the rate of the latter. Finally, it has been recently demonstrated that in aged cells one of the key aging-related processes previously considered irreversible, attenuation of the expression of a major stress response protein, Hsp70, can be reversed. This fact together with a probable regulatory role of the stress response potential in cellular aging suggested a possibility that the cellular aging process as a whole can be altered. Indeed, in the present study, growth on a denatured collagen matrices reversed in aged cells not only the attenuation of Hsp70 expression but also other aging-related processes, such as beta-galactosidase expression, increase in protein oxidation and changes in cell morphology. Moreover, it appeared to reduce the rate of aging in young cells. Understanding the nature of collagen matrix-mediated cellular rejuvenation might suggest approaches for interfering with organismic aging. Some immediate applications include cell rejuvenation for purposes of cloning and reduction of the rate of aging during expansion of stem cells for purposes of tissue engineering.

Cell Line↗
PubMed2002

Modifications in rejuvenation of the aging neck.

Surgical treatment of the aging neck should be approached according to the particular anatomic characteristics of the patient. One size does not fit all when speaking of cervicofacial rejuvenation. This article discusses some of the newer techniques used in rejuvenation of the aging neck.

Humans↗
PubMed1980

Oxygen consumption in perfused skeletal muscle. Effect of perfusion with aged, fresh and aged-rejuvenated erythrocytes on oxygen consumption, tissue metabolites and inhibition of glucose utilization by acetoacetate.

1. O2 consumption, glucose metabolism and the energy status of skeletal muscle were compared in isolated rat hindquarters perfused with aged (21--35 days), fresh and aged-rejuvenated human erythrocytes. 2. The age of the erythrocytes did not affect O2 consumption, glucose utilization or lactate release either at rest or during exercise. The concentrations of ATP, phosphocreatine and lactate within the muscle were also unaffected by the use of aged erythrocytes. 3. Perfusion with acetoacetate did not inhibit glucose utilization; but, it caused a marked increase in the tissue concentration of citrate in the soleus, a slow-twitch red muscle, and a smaller increase in the gastrocnemius, which contains fast-twitch red and white fibres. Results were similar in hindquarters perfused with aged and aged-rejuvenated erythrocytes. 4. These findings suggest that perfusion with aged human erythrocytes does not cause major alterations in the metabolic performance of the isolated rat hindquarter.

Acetoacetates↗
PubMed2000

Noninvasive rejuvenation of photodamaged skin using serial, full-face intense pulsed light treatments.

BACKGROUND: Photodamaged skin is characterized not only by rhytides, but also by epidermal and dermal atrophy, rough skin texture, irregular pigmentation, telangiectasias, laxity, and enlarged pores. There is growing interest in the development of noninvasive methods to treat photodamaged skin. Skin photorejuvenation is the visible improvement of photodamaged skin using a laser or other light source. A noncoherent, broadband, pulsed light source is effective in the treatment of vascular and pigmented lesions of the skin. This study evaluates the role of intense pulsed light in the rejuvenation of photo aged skin. OBJECTIVE: The purpose of this study was to evaluate and quantify the degree of visible improvement in photodamaged skin following a series of full-face, intense pulsed light treatments. METHODS: Forty-nine subjects with varying degrees of photo-damage were treated with a series of four or more full-face treatments at 3-week intervals using a nonablative, nonlaser intense pulsed visible light source. Fluences varied from 30 to 50 J/cm2. Subject evaluation and skin biopsies were used to assess treatment results. RESULTS: All aspects of photodamage including wrinkling, skin coarseness, irregular pigmentation, pore size, and telangiectasias showed visible improvement in more than 90% of subjects with minimal downtime and no scarring. Eighty-eight percent of subjects were satisfied with the overall results of their treatments. CONCLUSION: Treatment of photodamaged facial skin using a series of full-face treatments with intense pulsed light is a new and effective noninvasive method of skin rejuvenation with minimal risk and no patient downtime.

Adult↗
PubMedDate not supplied

Therapeutic effectiveness and safety of outdated human red blood cells rejuvenated to improve oxygen transport function, frozen for about 1.5 years at 80 C, washed, and stored at 4 C for 24 hours prior to rapid infusion.

After storage at 4 C for 20 to 28 days, red blood cells were biochemically modified to improve their oxygen transport function which had deteriorated during liquid storage. The solution used for rejuvenation contained pyruvate, inosine, glucose, phosphate, and adenine (PIGPA Solution B). The rejuvenated red blood cells were frozen with 40% W/V glycerol in a polyolefin plastic bag and were stored in the frozen state for about 1.5 years at -80 C. After thawing and washing the red blood cells were stored at 4 C in a sodium chloride-glucose-phosphate solution for 24 hours before transfusion. A pool of four to ten units was rapidly transfused to each of 14 elderly anemic recipients, 11 of whom had cardiopulmonary insufficiency. Recovery of the red blood cells after the freeze-thaw process was about 97 per cent, and after the freeze-thaw-wash process about 90 per cent. The 24-hour posttransfusion survival values were about 75 per cent, and the long-term survival values were about 85 days depending on the disease state of the recipient. The red blood cells had 1.5 times normal 2.3-DPG levels and a decreased affinity for oxygen at the time of transfusion and were able to delivery oxygen at high oxygen tension immediately after the rapid infusion of pools of from four to ten units through a 40-or 170-micron filter. Plasma hemoglobin levels were consistent with extravascular sequestration of nonviable red blood cells, and uric acid levels were not increased during the immediate 24-hour posttransfusion period.

Aged↗
PubMed1989

Phosphoenolpyruvate in the rejuvenation of stored red cells in SAGM medium: optimal conditions and the indirect effect of methemoglobin formation.

Red cells stored in saline-adenine-glucose-mannitol (SAGM) medium were rejuvenated by incubation with phosphoenolpyruvate (PEP) under conditions that can be achieved easily in ordinary blood banking. Regeneration of 2,3 diphosphoglycerate (2,3 DPG) and adenine nucleotides of stored red cells was dependent on the pH of the incubation medium and the incubation time. In red cells stored for 3 and 5 weeks, the optimal pH and incubation time for regeneration of 2,3 DPG and adenine nucleotides were 5.8 and 90 minutes and 6.1 and 60 minutes, respectively. During the incubation of red cells with PEP, methemoglobin was formed; it increased when the medium pH was below 6.0 and the incubation time exceeded 60 minutes. We conclude that incubation at a medium pH of 6.1 for 60 minutes is optimal for the rejuvenation of stored red cells with PEP. Under such incubation conditions, the concentrations of 2,3 DPG and adenine nucleotides in red cells stored for 5 weeks were restored to normal without methemoglobin formation.

2,3-Diphosphoglycerate↗
PubMed2000

New facial rejuvenation techniques.

The popularity of cutaneous laser resurfacing has soared in recent years. For optimal clinical improvement, patients have been limited to the carbon dioxide and erbium:yttrium aluminum garnet lasers. With these systems, tissue can effectively be ablated to induce collagen shrinkage and remodeling that result in an improved clinical appearance. The prolonged recovery periods associated with traditional cutaneous laser resurfacing have sparked an immense interest in devices that rejuvenate the skin while minimizing adverse effects. Both physicians and patients seem willing to accept more gradual improvement if it is associated with fewer complications such as prolonged erythema or delayed-onset hypopigmentation. The following review discusses the newest devices in development or currently available for skin rejuvenation.

Adult↗
PubMed2003

Midfacial rejuvenation: personal experiences and philosophy.

Procedures to rejuvenate the face are constantly evolving. Elevating the midfacial tissues in a vertical direction has gained recent popularity, as it seems to enhance results achieved from more traditional rhytidectomy techniques. Many methods have been described for performing this operation, and the differences among them range from subtle to profound. This article describes my experiences with some of these techniques and explains my personal evolution to the present. It is hoped that the reader will gain insight into the advantages and disadvantages of these surgical techniques and that he or she will be encouraged to explore various methodologies for rejuvenating the midface. The preferred surgical technique that is described herein should also serve as a stepping stone for those readers whose efforts will continue to expand our surgical options and enhance results in the future.

Aging↗
PubMed2004

Facial rejuvenation using botulinum toxin A: review and updates.

The aesthetic applications of botulinum toxin A (Botox) are well known to certain medical and nonmedical communities. This nonsurgical rejuvenation modality continues to be in high demand in part because of the immediacy of its effects, its high safety profile, and its no-downtime recovery period. The physician interested in using Botox for facial rejuvenation needs to have a working knowledge of facial anatomy and botulinum toxin pharmacology and clinical experience with Botox injections.

Botulinum Toxins, Type A↗
PubMed2004

Lipografting: autologous fat grafting for total facial rejuvenation.

Surgeons who perform facial rejuvenation procedures have long recognized the importance of volume enhancement. The primary manifestation of aging is loss of subcutaneous tissue, especially the atrophy of fat, which directly contributes to laxity of skin. Autologous fat transplantation has been attempted for over a century with varying degrees of success but more recent techniques have provided more predictable, reproducible, and even permanent results. Fat is an ideal tissue filler substance because it is living tissue and because it is from the patient's own body it is nonallergenic. My technique has evolved over an 11-year period in more than 1800 patients. The procedure can be performed alone as a total facial rejuvenation or in combination with facelift or other facial surgery. The technique requires minimally traumatic fat harvesting, fat preparation, and multilevel facial infiltration. Excess fat not used in the initial procedure is frozen and stored at -16 degrees C and can be used successfully for minor touch-up procedures. Preoperative evaluation of the patient and their understanding of the benefits and limitations of volume restoration surgery is imperative. Structural improvement to the main support areas of the face in the appropriate aesthetic proportions is of prime importance.

Adipose Tissue↗
PubMed2006

Thymic rejuvenation and the induction of tolerance by adult thymic grafts.

The thymus, the site of origin of T cell immunity, shapes the repertoire of T cell reactivity through positive selection of developing T cells and prevents autoimmunity through negative selection of autoreactive T cells. Previous studies have demonstrated an important role for the thymus not only in central deletional tolerance, but also in the induction of peripheral tolerance by vascularized renal allografts in juvenile miniature swine recipients. The same protocol did not induce tolerance in thymectomized recipients nor in recipients beyond the age of thymic involution. We subsequently reported that vascularized thymic lobe grafts from juvenile donors were capable of inducing tolerance in thymectomized juvenile hosts. However, the important question remained whether aged, involuted thymus could also induce tolerance if transplanted into thymectomized hosts, which, if true, would imply that thymic involution is not an intrinsic property of thymic tissue but is rather determined by host factors extrinsic to the thymus. We report here that aged, involuted thymus transplanted as a vascularized graft into juvenile recipients leads to rejuvenation of both thymic structure and function, suggesting that factors extrinsic to the thymus are capable of restoring juvenile thymic function to aged recipients. We show furthermore that rejuvenated aged thymus has the ability to induce transplant tolerance across class I MHC barriers. These findings indicate that it may be possible to manipulate thymic function in adults to induce transplantation tolerance after the age of thymic involution.

Aging↗
PubMed2002

Cervicoplasty and facial laser resurfacing: a paired technique for facial rejuvenation.

OBJECTIVE: A specific group of patients interested in facial rejuvenation was identified in a plastic surgery practice. These patients were primarily concerned with improving their neck contour and correcting their facial rhytides. To address those problems a one-stage paired technique was designed - cervicoplasty with full-face laser resurfacing. MATERIALS AND METHODS: Thirteen patients were treated with this procedure during a 1 year period. RESULTS: The patients experienced no significant complications and were extremely satisfied with their results. A retrospective study of 206 patients over the same year period revealed that the group undergoing the combination procedure: 1) was identifiable by the ranking of their specific aesthetic concerns; 2) was averse to undergoing a full facelift; and 3) comprised a significant percentage (22%) of surgical patients in the study period. CONCLUSIONS: Combining cervicoplasty with full-face laser resurfacing is a safe, effective procedure that provides another option in the realm of facial rejuvenation.

Aged↗
PubMed2003

Botulinum toxin in association with other rejuvenation methods.

Botulinum toxin is an import agent in facial rejuvenation, and the use of botulinum toxin in combination with other methods helps preserve and maintain a long-term result. This paper briefly reviews the use of botulinum toxin in associate with dermal fillers, laser resurfacing and other surgical rejuvenation modalities.

Botulinum Toxins, Type A↗
PubMed2004

Clinical evaluation of non-ablative radiofrequency facial rejuvenation.

OBJECTIVE: To evaluate the clinical efficacy of non-ablative cutaneous radiofrequency (RF) facial rejuvenation. DESIGN: Prospective study with longitudinal follow-up of a validation cohort set in an urban, private practice of an ambulatory facial plastic surgery center in southern California. METHODS: A consecutive sample was enrolled of 35 healthy adults with moderate facial aging, manifested by skin laxity, rhytids, and ptosis (brow, midface, jowls). Following intravenous sedation anesthesia, the study area was treated with 115-144 J/cm2 using the non-ablative RF device (ThermaCool TC). The main outcome measures were the objective measurement of brow height, investigator evaluation of skin parameters, a patient satisfaction questionnaire, and standardized photography, at fixed time intervals. RESULTS: At 12 weeks, a statistically significant increase in mean vertical brow height of 1.6-2.4 mm was observed in patients treated exclusively with the RF device (p<0.0001). All skin parameters (laxity, wrinkles, clarity, pore size) were improved. Complications and side effects were minimal. Patients were uniformly satisfied. CONCLUSIONS: The ThermaCool TC RF system represents a promising non-invasive method of obtaining moderate facial rejuvenation in the appropriately selected patient. Long-term results are pending.

Adult↗
PubMed1991

Rejuvenation of erythrocytes preserved with AS-1 and AS-3.

Rejuvenation of erythrocytes (biochemical modification of adenosine triphosphate and 2,3-diphosphoglycerate) preserved with currently available nutrient additive solutions has not been licensed or adequately studied. The ability of the commercially available solution PIPA to modify erythrocytes preserved with AS-1 or AS-3 for 35 and 42 days was studied. Use of PIPA produced greater-than-normal levels of adenosine triphosphate in the preserved erythrocytes. Restoration of 2,3-diphosphoglycerate was suboptimal at both periods. (PIPA treatment produces normal levels of 2,3-diphosphoglycerate in erythrocytes preserved for 35 days with citrate-phosphate-dextrose-adenine-1.) In erythrocytes preserved with AS-1 or AS-3 for 42 days, 2,3-diphosphoglycerate returned to normal levels after a second PIPA rejuvenation. This study demonstrates that biochemical modification is possible in erythrocytes preserved with AS-1 or AS-3 for 42 days.

2,3-Diphosphoglycerate↗