Search PubMed⌕ Search

SEARCH · Search PubMed

Results for “Poly G”

Search indexed PubMed citations on genomics, clinical trials, systematic reviews and public health. Explore titles, authors and supplied subject terms, then open the PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 109 records · Page 6Linked to original sources
PubMed1988

A spectroscopic study of the conformation of poly d(G-C).poly d(G-C) modified with the carcinogenic 2-aminofluorene.

The conformational properties of both the B- and Z-form helix of the 2-aminofluorene (AF) modified synthetic polynucleotide poly d(G-C).poly d(G-C) were extensively studied with a variety of optical techniques (UV absorbance, linear dichroism, circular dichroism and fluorescence spectroscopy). The spectroscopic results show, that: (i) the presence of the AF adduct in poly d(G-C).poly d(G-C) facilitates the conversion of the right-handed B-form polynucleotide to the left-handed Z-type poly d(G-C).poly d(G-C) duplex; (ii) in B- as well as in Z-form poly d(G-C).poly d(G-C)-AF the carcinogenic residue is situated in a specific and ordered complex with the DNA, in which the AF chromophore is significantly immobilized and the long axis of the fluorene ring system is positioned in a parallel mode to the DNA base planes; (iii) in B-type poly d(G-C).poly d(G-C)-AF the carcinogenic residue exhibits strong stacking interactions with the adjacent DNA bases, which are coupled to an important destabilization of the AF-containing B-duplex; (iv) in contrast to the B-form complex, the AF-containing Z-type DNA is highly stabilized and a remarkable reduction of the AF-base interactions is observed; (v) in Z-form poly d(G-C).poly d(G-C)-AF the AF chromophore resides at a medium-exposed position. The combined data support a conformational model, in which the planar AF is inserted in the B-type polynucleotide helix, while the carcinogenic residue in the Z-form poly d(G-C).poly d(G-C)-AF structure is placed in an outside position.

Carcinogens↗
PubMed2006

Synthesis and characterization of chitosan-g-poly(ethylene glycol)-folate as a non-viral carrier for tumor-targeted gene delivery.

Poor water solubility and low transfection efficiency of chitosan are major drawbacks for its use as a gene delivery carrier. PEGylation can increase its solubility, and folate conjugation may improve gene transfection efficiency due to promoted uptake of folate receptor-bearing tumor cells. The aim of this study was to synthesize and characterize folate-poly(ethylene glycol)-grafted chitosan (FA-PEG-Chi) for targeted plasmid DNA delivery to tumor cells. Gel electrophoresis study showed strong DNA binding ability of modified chitosan. The pH(50) values, defined as the pH when the transmittance of a polymer solution at 600 nm has reached 50% of the original value, suggested that the water solubility of PEGylated chitosan had improved significantly. Regression analysis of pH(50) value as a function of substitution degree of PEG yielded an almost linear correlation for PEG-Chi and FA-PEG-Chi. The solubility of PEGylated chitosan decreased slightly by further conjugation of folic acid due to the relatively more hydrophobic nature of folic acid when compared to PEG. In addition, the chitosan-based DNA complexes did not induce remarkable cytotoxicity against HEK 293 cells. FA-PEG-Chi can be a promising gene carrier due to its solubility in physiological pH, efficiency in condensing DNA, low cytotoxicity and targeting ability.

Carrier Proteins↗
PubMed2005

Pluronic-g-poly(acrylic acid) copolymers as novel excipients for site specific, sustained release tablets.

Potential utility of copolymers comprising Pluronic (PEO-PPO-PEO) surfactants covalently conjugated with poly(acrylic acid) (PAA) as excipients for sustained-release tablets was explored. Apparent particle density, particle size distribution, Carr index, thermal stability, and compression behavior of the Pluronic-PAA copolymers were characterized. Tablets prepared by direct compression of blends of Pluronic-PAA copolymers were evaluated on the basis of their thermomechanical profile, crushing strength, friability, and drug release properties. Small molecular weight drugs of aqueous solubility decreasing in the order theophylline>hydrochlorothiazide>nitrofurantoin were incorporated to the tablets. For comparison purposes, tablets were also prepared from PAA of Carbopol 71G (C71G), and mixtures of C71G and Pluronic F127, with each of the above three drugs. The Pluronic-PAA aggregates are stabilized by hydrophobic associations between poly(propylene oxide) (PPO) segments in aqueous solutions, and thus require higher ionization of the carboxylic groups to overcome the associations and swell. The swelling pattern of the Pluronic-PAA copolymers is more dramatically pH-dependent than that of Carbopol lacking any hydrophobic associations. The drug retention in and release from the Pluronic-PAA based tablets is profoundly pH-dependent and hence specific to the pH exceeding that of the pK(a)>5 of these copolymers. Theophylline- and hydrochlorotiazide-containing tablets made with Pluronic-PAA copolymers showed a reduced release rate under acidic conditions compared to the neutral or alkaline conditions, while the opposite pattern was observed with the Carbopol-based tablets due to the different pH-dependent swelling behavior of the polymers. Nitrofurantoin-containing tablets showed a remarkably low drug release rate owing to the strong hydrophobic character of nitrofurantoin and of its complexes with the copolymers. Integrity of the nitrofurantoin-containing tablets was maintained during the 24h release test. Zero-order kinetics of the cumulative release profile of all drugs under study was observed with the Pluronic-PAA as a tablet excipient. Adequate mechanical properties, the self-assembling behavior, and the pH-sensitiveness of the Pluronic-PAA copolymers make them promising excipients for tablets with preferential delivery into a neutral to alkaline pH environment.

Acrylic Resins↗
PubMed2004

Solubilization and stabilization of camptothecin in micellar solutions of pluronic-g-poly(acrylic acid) copolymers.

The capability of a family of copolymers comprising Pluronic (PEO-PPO-PEO) surfactants covalently conjugated with poly(acrylic acid) (Pluronic-PAA) to enhance the aqueous solubility and stability of the lactone form of camptothecin (CPT) was studied. The unprotected lactone form of CPT, which possesses cytotoxic activity, is rapidly converted to the ring-opened carboxylate form under physiological conditions. Firstly, surfactant properties such as critical micellization concentration (CMC) of Pluronic-PAA copolymers were characterized. Then, the equilibrium solubility partitioning and hydrolysis of the lactone form of CPT in the presence of Pluronic-PAA in water and in human serum were analyzed. CPT solubility in polymer micellar solutions was ca. 3- to 4-fold higher than that in water at pH 5. The amount of CPT solubilized per PPO was considerably greater in the Pluronic-PAA solutions than in the parent Pluronic solution, which suggests that the drug is not only solubilized by the hydrophobic cores and also by the hydrophilic POE-PAA shells of the micelles. The equilibrium partition coefficient of the CPT lactone between Pluronic-PAA solutions and water exceeded (2-3) x 10(3). The complete solubilization of CPT and the absence of chemical interactions between CPT and Pluronic-PAA were confirmed by modulated temperature differential scanning calorimetry (MTDSC), infrared spectroscopy, and X-ray diffraction of films. The loading of CPT into the Pluronic-PAA micelles was able to prevent the hydrolysis of the lactone group of the drug for 2 h at pH 8 in water. When compared to the unprotected CPT, the kinetics of the CPT hydrolysis in human serum was about 10-fold slower in the Pluronic-PAA formulations.

Acrylic Resins↗
PubMed2001

Band resonant tunneling in DNA molecules.

The charge migration process in DNA is subject to a lively debate among researchers at the moment. We have performed calculations on poly(G)-poly(C) DNA which substantiate a recent report indicating bandlike conduction for this particular form of DNA. Our results show that both guanine and cytosine give rise to conducting channels along the DNA strands. The conductivity results from the overlap of the pi orbitals along the base stacks. We also demonstrate that the measured increase of the threshold voltage with temperature in poly(G)-poly(C) DNA is the result of electron localization due to the structural disorder following high temperature.

DNA↗
PubMed1988

In vitro excision of adeno-associated virus DNA from recombinant plasmids: isolation of an enzyme fraction from HeLa cells that cleaves DNA at poly(G) sequences.

When circular recombinant plasmids containing adeno-associated virus (AAV) DNA sequences are transfected into human cells, the AAV provirus is rescued. Using these circular AAV plasmids as substrates, we isolated an enzyme fraction from HeLa cell nuclear extracts that excises intact AAV DNA in vitro from vector DNA and produces linear DNA products. The recognition signal for the enzyme is a polypurine-polypyrimidine sequence which is at least 9 residues long and rich in G.C base pairs. Such sequences are present in AAV recombinant plasmids as part of the first 15 base pairs of the AAV terminal repeat and in some cases as the result of cloning the AAV genome by G.C tailing. The isolated enzyme fraction does not have significant endonucleolytic activity on single-stranded or double-stranded DNA. Plasmid DNA that is transfected into tissue culture cells is cleaved in vivo to produce a pattern of DNA fragments similar to that seen with purified enzyme in vitro. The activity has been called endo R for rescue, and its behavior suggests that it may have a role in recombination of cellular chromosomes.

DNA, Viral↗
PubMedDate not supplied

[Combined use of a specific vaccine and interferon inducers for preventing and treating experimental tick-borne encephalitis].

A comparative study of prophylactic and therapeutic activity of 6 Soviet interferon inducers in tick-borne encephalitis was carried out. Such inducers as double-stranded RNA, poly(G) . poly(C), and tyloron were most active prophylactically. A therapeutic effect was observed after combined use of specific vaccine and interferon inducers: double-stranded RNA and poly(G) . poly(C) which underwent clinical trials. When these inducers were administered 3 hours after infection the protective effect was 56.7% and 46%, respectively.

Animals↗
PubMed2006

Affinity separation of polyribonucleotide-binding human blood proteins.

Using affinity columns with immobilized poly(A), poly(G), poly(U), poly(C), and poly(A).poly(U) and poly(G) x poly(C) duplexes several polyribonucleotide-binding blood plasma proteins have been captured. Albumin and keratins K1 and K2e have been detected to bind polypurine tracts. The in vitro glycated albumin binds poly(A) and poly(G) more efficiently than the unmodified protein. The major polypyrimidine-binding blood plasma protein (28 kDa) can catalyze the hydrolysis of poly(U).

Blood Proteins↗
PubMed1983

Conformational transitions in the synthetic polynucleotide poly[d(G-C)] . poly[d(G-C)] double-helix.

Conditions are described for observing by X-ray fibre diffraction the A, B and S conformations of the poly[d(G-C)] . poly[d(G-C)] double-helix and also a new form designated as B". For fibres with an appropriate ionic content, transitions between these conformations can be induced by varying the relative humidity of the fibre environment. With increasing relative humidity the transitions B" leads to A leads to S leads to B occur. However, reducing the relative humidity does not result in a simple reversal of these transitions. If the relative humidity is reduced rapidly, a B leads to A transition is observed followed by an A leads to B" transition, but if it is reduced slowly, the transition is from B to S. Once the S form has been assumed, further reduction in the relative humidity does not result in a transition to the A form. The S form emerges as a particularly stable form of the poly[d(G-C)] . poly[d(G-C)] double-helix. From the point of view of its relationship to the classical A and B forms, the S form of poly[d(G-C)] . poly[d(G-C)] is shown to exhibit similarities to the D form of poly[d(A-T)] . poly[d(A-T)].

Humidity↗
PubMed1983

Interactions of nuclear estrogen receptor with DNA and RNA.

The interaction of the nuclear estrogen receptor from hen oviduct with nucleic acids were studied by competition assay using DNa-cellulose centrifugation. We demonstrated that the estradiol-receptor complex binds similarly well to poly(A) RNA and denatured DNA. The estrogen receptor was found to interact more strongly with poly(G), poly(U) than with poly(A), poly(C). The receptor complex binds similarly to poly(A) and poly(dA), and to poly(U) and poly(dU). However, the receptor complex shows stronger binding to poly(G) than to poly(dG) and to poly(C) than to poly(dC). Studies with heteropolyribonucleotides indicated that poly(U1G1) is more effective in competing for the estrogen receptor, and poly(AC) and poly(AUG) are moderately effective, whereas poly(ACU) is least effective. GMP and dGMP showed some competition for the nuclear receptor at 300-fold higher nucleotide concentrations than that of the synthetic poly(G). Observations that the nuclear estrogen receptor binds to poly(A) RNA and interacts selectively with polyribonucleotides suggest that the estrogen receptor-RNA interaction may play a role for the function of estrogens in gene regulation.

Animals↗
PubMed1989

Raison d'être and structural model for the B-Z transition of poly d(G-C).poly d(G-C).

In DNA oligonucleotides crystallized in the A form, the nucleotides adopt standard conformation except for steps 5'-CpG-3' where reduced base-pair twist and a sliding motion of the base pairs along their long axes causes pronounced interstrand guanine-guanine overlap. As a consequence, torsion angles alpha, beta and gamma are consistently trans, trans, trans instead of the common-gauche, trans, +gauche. This conformation significantly increases the intraresidue distance between the guanine base and the 5'-phosphate group. A molecular model of poly d(G-C).poly d(G-C) built with these structural characteristics in the A form, which we call A2-DNA, shows that rotation of the guanosine sugar into the syn orientation is easily achieved and pushes the base pair across the helix axis. If successive guanosines are changed this way, a smooth transformation occurs to the left-handed Z-DNA. We suggest that A- and A2-DNA forms of poly d(G-C).poly d(G-C) are metastable and that the actual transition is B in equilibrium (A in equilibrium A2) in equilibrium Z-DNA.

Crystallography↗
PubMed1986

Cyclic voltammetry of DNA at a mercury electrode: an anodic peak specific for guanine.

Synthetic homopolyribonucleotides poly(A), poly(U), poly(C), and poly(G), poly(A, G, U), apurinic acid and native and denatured DNA from calf thymus were analyzed by means of cyclic voltammetry (CV) using a hanging mercury drop electrode. It was shown that guanine containing polynucleotides, i.e. poly(G), poly(A, G, U) and DNA yield an anodic peak of guanine in the vicinity of a potential of -0.3 V (against a saturated calomel electrode). The guanine peak appeared only at a sufficiently negative switching potential (about -2 V). The appearance of the guanine peak was conditioned by a reduction of guanine residues in the region of the switching potential and reoxidation of the reduction product in the vicinity of -0.3 V. Native and thermally denatured DNAs were investigated under the conditions of both complete and incomplete coverage of the electrode in various background electrolytes. Both DNA forms yielded anodic CV peaks of guanine with the peak of denatured DNA being always higher than that of native DNA. Irradiation of native DNA with relatively small doses of gamma radiation (5-120 Gy) resulted in an increase of the anodic peak. A comparison of changes induced by gamma radiation in the anodic (guanine) and cathodic (reduction of adenine and cytosine) peaks showed a steeper increase of the cathodic peak as compared to that of the anodic one. It has been concluded that in the given dose range the DNA double-helical structure is mainly damaged in the adenine-thymine rich regions.

Apurinic Acid↗