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At least 109 records · Page 6Linked to original sources

Tracheobronchial injuries after blunt chest trauma in children--hidden pathology.

BACKGROUND: Blunt thoracic injuries in children are unique because the pliability of the chest wall allows transmission of massive external force directly into the mediastinum. Children presenting after blunt chest trauma may have complete disruption of the airway with little external sign of injury. Without prompt diagnosis and appropriate treatment, the risk for progressive respiratory failure is high. METHODS: Four children with tracheobronchial injuries were referred to a pediatric trauma center from 1994 to 1997. All children, age 18 months to 13 years, suffered unusual crush injuries. All diagnoses were based on unresolved pneumothorax or pneumomediastinum. RESULTS: Bronchoscopy identified the location of injury as posterior trachea (n = 1) and right mainstem bronchus (n = 2). A tertiary bronchial injury (n = 1) was missed by initial tracheogram and subsequent bronchoscopy but identified during surgical exploration. All children survived after thoracotomy and primary repair of the injury. CONCLUSIONS: Tracheobronchial disruption is a rare, life-threatening injury. Suspicion should be high when pneumomediastinum and pneumothorax are refractory to adequate pleural drainage. Flexible bronchoscopy with intubation distal to the injury may be necessary to prevent loss of the airway. Advance preparation should include setups for bronchoscopy, thoracotomy, and cardiopulmonary bypass. Patient survival depends on preparation and prompt surgical intervention.

Adolescent↗

Complications of implant surgery in children.

Rapid recognition and appropriate therapy will prevent or remediate most of the complications occurring in children after cataract aspiration and IOL implantation. Some of the complications of pediatric IOL implantations are related to the increased scleral pliability and decreased rigidity which predispose scleral collapse, vitreous loss, flat anterior chamber and corneal endothelial damage. A second group of complications is associated with the enhanced inflammatory and fibrotic responses peculiar to a child's eye. This group includes operative striate keratitis and iridocyclitis, late IOL precipitates, secondary and postpseudophakos membranes, iris erosion and synechiae formation, and IOL tilt and displacement. Although the intraocular lens is a possible means of visual rehabilitation for children with traumatic cataracts or unilateral infantile cataracts, its long-term risk/benefit ratio must still stand the test of time.

Adolescent↗

Use of agar in ophthalmic pathology: a technique to improve the handling and diagnosis of temporal artery biopsies, subfoveal membranes, lens capsules, and other ocular tissues.

DESIGN: Prospective laboratory methodology study. PURPOSE: Certain tissues, by virtue of their shape and extreme thinness or pliability, are difficult to position correctly during routine paraffin embedding to provide the optimal orientation for histopathologic studies. Biopsy specimens from temporal arteries must be sampled at different points along the length of the artery. Other tissues such as subfoveal neovascular membranes and fragments of lens capsule lack the thickness and rigidity to be positioned on edge to yield cross-sectional views. The authors' technique improves the orientation and thereby maximizes the histologic information obtained from such specimens. METHODS AND MATERIALS: From January 1, 1990, to April 30, 1999, the authors studied 500 consecutive temporal artery biopsy specimens and 200 successive subfoveal neovascular membranes. RESULTS: Cutting a 20-mm cylindrical fragment of temporal artery at 1- to 1.5-mm intervals yielded approximately 13 to 20 cross-sections along the length of the artery. When the specimens were positioned together and embedded in agar, the pathologist could easily study multiple cross-sections of the artery. Additionally, using the agar technique, the authors were able to obtain cross-sections of other specimens submitted, such as subfoveal neovascular membranes, and studied each of the different layers to evaluate the disease process. By the same method, the authors placed small fragments of lens capsule with underlying cortex on edge and readily identified short, gram-positive coccobacilli consistent with Propionibacterium acnes endophthalmitis. CONCLUSION: The agar technique can greatly improve the quality of diagnostic information gleaned from temporal artery biopsy specimens and other small tissue samples.

Agar↗

The effect of a tissue engineered bilayered living skin analog, over meshed split-thickness autografts on the healing of excised burn wounds. The Apligraf Burn Study Group.

The potential of Apligraf(R) (Graftskin) (Organogenesis Inc., Canton, MA), to improve cosmetic and functional outcomes when applied over meshed split thickness autografts was evaluated in a multicenter, randomized within patient controlled clinical trial. Experimental treatment sites had Apligraf(R) placed over meshed autograft while control sites were treated with meshed autograft covered with meshed allograft, or meshed autograft not covered by a biologic dressing. Forty patients were entered into this study of which 38 were evaluable. There was no difference in the percent take of autograft in the presence or absence of Apligraf(R) or in the median number of days to greater than 75% take of autograft. At the completion of the study 22 (58%) of the Apligraf(R) sites were rated superior to the control sites by the investigators, 10 (26%) were rated equivalent to the control and six (16%) were rated worse than control (p=0. 0037). Pigmentation, in the Apligraf(R) group was significantly better than control and by month 24, 17 (45%) Apligraf(R) sites had normal pigmentation compared with five (13%) control sites (p=0. 0005). Similarly by month 24, 18 (47%) patients had normal vascularity at the Apligraf(R) site compared with six (16%) patients at the control site. Improvements in pliability were observed with Apligraf(R) over control treatment within the first week of treatment. At month 24, 23 (61%) patients had normal height at the Apligraf(R) site compared to 14 (37%) with normal height at the control site (p=0.0117). Vancouver burn scar scores were shown to be statistically better at Apligraf(R) sites compared to control at all time points from week 1 to month 24. These results indicate that Apligraf(R) is a suitable and clinically effective treatment for burn wounds when applied over meshed autografts. Furthermore, cosmetic and functional advantages with Apligraf(R) were demonstrated when applied over meshed autograft compared to the current standard treatments of meshed autografts.

Adolescent↗

A randomised clinical trial comparing a hydrocolloid-derived dressing and glycerol preserved allograft skin in the management of partial thickness burns.

Membranous dressings for the treatment of partial and mixed thickness burns are among the most innovative and promising new developments of the last years. In this study, we present data of a randomised prospective comparative study on a carboxymethylcellulose based dressing, Hydrofibre((R)) and glycerolized human allograft skin. In a 2 year period, 80 patients (40 for each material) were enrolled in the trial. Study wounds (<10% TBSA) that had not re-epithelialised after 14+/-3 days were debrided and grafted or, if small enough, managed with a topical antimicrobial agent. Mean total TBSA was 8.3+/-5.2%, study burn 3.7+/-2.0% for the Hydrofibre((R)) group and 7.3+/-4.3% total, 3.4+/-2.1% study burn for the allograft skin group (n.s. Wilcoxon rank sum test). No significant differences between groups were established in number of patients with superficial/deep burns. In both groups about 2/3 of the patients healed completely with the dressings applied (24/40 versus 27/40 for Hydrofibre((R)) versus allograft skin, respectively). However, a higher incidence of post-study excision and grafting was found in the Hydrofibre((R)) group (45% versus 15% in the allograft skin group, P=0.004, Mann-Whitney). At 10 weeks follow-up no significant differences were seen in scar colour, pigmentation, pliability, height or itching (Vancouver Scar Scale). Skin elasticity, measured by the Cutometer((R)), was significantly better for the allograft group (P=0.010, Wilcoxon). These differences were no longer found at 6 months and 1 year follow-up. Incidence of hypertrophy after 6 months was higher, but not significantly, in the Hydrofibre((R)) compared to the allograft skin group (52.5% versus 30%, P=0.09, chi-square). In view of the results from our comparative study on Hydrofibre((R)) versus allograft skin, we prefer the use of allograft skin for the category of larger burns of mixed depth, usually presented to burn centres. However, for partial thickness and small burns Hydrofibre((R)) can be the first choice in treatment.

Adult↗

[Gas embolism and flexible Ecoflac type bag].

Gas embolism at the end of infusion is a well known hazard, that should have disappeared with the use of flexible bags. However, some cases have been reported after pressure infusion. This experimental study evaluates the risk for gas embolism with Ecoflac type flexible bags. These bags are safe under normal pressure infusion conditions with a pneumatic sleeve, because of their texture and pliability; indeed, only minimal air volumes could be expelled, without any risk even in children. However, to be on the safe side, the manufacturer recommends to expel any residual air before pressure administration. This recommendation applies to any bag containing residual air, and since many people are not aware of this, it is rarely put into practice.

Child↗

Eight years clinical experience with the replacement of the ascending aorta using composite xenopericardial conduit.

OBJECTIVE: The evaluation of early and late results after ascending aorta replacement with composite glutaraldehyde-treated xenopericardial valved conduit. METHODS: From December 1989 to May 1999 the ascending aorta was replaced with 148 composite xenopericardial conduits aorta in 145 patients. Biological valves were inserted in 28 conduits, mechanical valves - in 116. The age of 40 female (28%) and 105 male (72%) patients ranged from 10 to 60 years (mean, 38.7+/-12 years). The original diseases were: atherosclerosis in 51 (35%), cystic media necrosis in 50 (35%), Marfan syndrome in 35 (24%), syphilis in three (2%), non-specific aortitis in one (0.7%), Turner syndrome in one and infective endocarditis in four cases. Aortic dissection was found in 67 patients (46%): type I in 14 (23%) and type II in 53 (77%). Twenty-one patients (15%) were operated on during the acute phase of the dissection. The Bentall-DeBono technique was used in 144 operations, in four cases (2.7%) supracoronary resection was performed. Associated procedures included: mitral valve repair (five), CABG (four), resection of the coarctation (two), MV replacement (two). Biological tissues condition was assessed using TTE TEE and computed tomography (CT) scanning technique. RESULTS: Hospital mortality was 8.3 % (12 patients). The death was caused by: low cardiac output (three), arrhythmia (two), neurological complications (one), sepsis (one), polyorgan failure (four), bleeding (one). Non-lethal complications included: bleeding (four), heart failure (two), persisting A-V block (two), polyorgan failure (three), cerebral (two), mediastinitis (three), and early prosthetic endocarditis in three patients. All three patients with endocarditis were successfully re-operated and conduit replaced with the same type of the device. Late follow-up ranged from 2 months to 8 years (mean, 51 months) and complete in 87% of the discharged patients (115 patients). There were four valve-related deaths due to thrombembolia and five deaths non related to the valve and/or conduit. Clinical and instrumental evaluation did not reveal any signs of tissue degeneration at the conduit and biovalves' cusps. CONCLUSIONS: The xenopericardial valved conduit is an effective and acceptable device for the replacement of ascending aorta in almost all cases, it provides good early and late results. It's pliability and elasticity are especially attractive in situations with frail tissues and high risk of bleeding.

Adolescent↗

Role of an operative score in mitral reconstruction in dominantly stenotic lesions.

OBJECTIVE: The role of an operative score in selection and results of mitral valve reconstruction in dominantly stenotic mitral lesions was assessed. METHODS: A total of 136 patients consecutively underwent reconstruction for rheumatic mitral stenosis with or without regurgitation from December 1989 through December 1994. Mitral valve structure was scored for cuspal pliability and thickness, cuspal area loss, chordal length, papillary muscle length, annular dilatation and degree of associated regurgitation and calcification. A score of 0 indicated normal valve while a score of 24 indicated a grossly deformed valve. Age, sex, functional class, cardiac rhythm, mitral valve orifice area, left ventricular and left atrial dimensions, transmitral gradients, pulmonary artery pressures and thoroughness of repair were evaluated. RESULTS: Mitral valve architecture was disorganised moderately in 50 (37%) and severely in 69 (52%) patients. Extent of thoroughness in different operative techniques correlated with good (n = 30) or adequate (n = 60) outcome. Mean mitral valve orifice area increased from 0.77 +/- 0.2 to 2.56 +/- 0.6 cm2, peak gradient dropped from 20.3 +/- 6.2 to 8.6 +/- 3.5 torr, mean gradient from 13.5 +/- 4.9 to 4.76 +/- 2.2 torr and end-diastolic gradient from 10.1 +/- 5.0 to 3.2 +/- 1.9 torr. Lack of recognition of anatomical details and of all necessary componental measures constituted the learning curve of different operators. CONCLUSIONS: Recurrent disease, learning curve, inadequate repair and higher operative mitral valve score were the factors for poorer results in the intermediate term follow-up to 64 months. Thoroughness of repair was the most important correlate of outcome and indicative of expertise.

Adolescent↗

Transpositional adipofascial flaps for complicated acute finger injries.

Seven patients who presented with complicated acute finger injuries were treated successfully with local transpositional adipofascial flaps. This is a one-stage procedure with advantages of simplicity and rapidity. This flap is thin with good pliability and provides a very versatile and reliable covering for soft-tissue defects of the fingers. Full-thickness skin grafts were applied to the raw surface of the adipofascial flaps in all patients. All cases had excellent wound healing and restored full range of motion. This procedure facilitates early wound healing and early range of motion for finger joints; it also reduces wound pain, minimizes scar formation, and eliminates the need for second-stage surgery.

Acute Disease↗

Internal and external mass transfer in biofilms grown at various flow velocities.

It appears that biofilms arrange their internal structure according to the flow velocity at which they are grown, which affects the internal mass transfer rate and microbial activity. In biofilms grown at various flow velocities we determined the vertical profiles of the local relative effective diffusivity (termed D(l)) at several locations within each biofilm. From these profiles we calculated the surface-averaged relative effective diffusivity (termed D(sa)) at various distances from the bottom and plotted it against these distances. The D(sa) decreased linearly toward the bottom, forming well-defined profiles that were different for each biofilm. The gradients of these profiles were multiplied by the diffusivity of oxygen, zeta = D(w) dD(sa)/dz, and plotted versus the flow velocity at which each biofilm was grown. The gradients were low at flow velocities below 10 cm/s, reached a maximum at a flow velocity of 10 cm/s, and decreased again at flow velocities exceeding 10 cm/s. The existence of a maximum indicates a possibility that two opposing forces were affecting the slope of the profiles. To explain these observations we hypothesized that biofilms, depending on the flow velocity at which they are grown, arrange their internal architecture to control (1) the nutrient transport rate and (2) the mechanical pliability needed to resist the shear stress of the water flowing past them. It appears that biofilms attempt to satisfy the second goal first, to increase their mechanical strength, and that they do so at the expense of the nutrient transfer rate to deeper layers. This strength increase is associated with an increase in biofilm density, which slows down the internal mass transport rate. Biofilms grown at low flow velocities exhibit low density and high effective diffusivity but cannot resist higher shear stress, whereas biofilms grown at higher flow velocities are denser and can resist higher shear stress but have a lower effective diffusivity.

Biofilms↗

Structure of the deoxymyoglobin model [Fe(TPP)(2-MeHIm)] reveals unusual porphyrin core distortions.

The preparation and characterization of the deoxymyoglobin model (2-methylimidazole)(tetraphenylporphinato)iron(II) is described. The preparation and crystallization from chlorobenzene leads to a new crystalline phase that has been structurally characterized. The complex is the most ordered example of a deoxymyoglobin model yet characterized. The X-ray structure determination reveals a number of distortions both in the iron coordination group and in the porphyrin core that result from the steric bulk of the axial ligand. Some of these distortions have been noted previously in related species; however, the demonstration of porphyrin core distortions and an asymmetry in the Fe-N(p) bond distances are new observations. These may have functional significance for this important type of heme protein coordination group. The new structure emphasizes that high-spin iron(II) porphyrinate derivatives display substantial structural pliability with significant variations in iron atom displacements, porphyrin core hole size, and axial and equatorial Fe-N bond lengths. The new complex has also been characterized by zero-field and applied field magnetic Mössbauer spectroscopy. Mössbauer parameters are characteristic for high-spin iron, although they also reveal an extremely rhombic site for iron(II). Crystal data at 130 K for [Fe(TPP)(2-MeHIm)].1.5C(6)H(5)Cl: a = 12.334(3) A, b = 13.515(6) A, c = 14.241(7) A, alpha = 70.62(3) degrees, beta = 88.29(2) degrees, gamma = 88.24(3) degrees, triclinic, space group, P, V = 2238(2) A(3), Z = 2.

Crystallography, X-Ray↗

Hierarchical assembly of cell-matrix adhesion complexes.

The adhesion of cells to the extracellular matrix is a dynamic process, mediated by a series of cell-surface and matrix-associated molecules that interact with each other in a spatially and temporally regulated manner. These interactions play a major role in tissue formation, cellular migration and the induction of adhesion-mediated transmembrane signals. In this paper, we show that the formation of matrix adhesions is a hierarchical process, consisting of several sequential molecular events. One of the earliest steps in surface recognition is mediated, in some cells, by a 1 microm-thick cell-surface hyaluronan coat, which precedes the establishment of stable, cytoskeleton-associated adhesions. The earliest forms of these integrin-mediated contacts are dot-shaped FXs (focal complexes), which are formed under the protrusive lamellipodium of migrating cells. These adhesions recruit, sequentially, different anchor proteins that are involved in binding the actin cytoskeleton to the membrane. Conspicuous in its absence from FXs is zyxin, which is recruited to these sites only on retraction of the leading edge and the transformation of the FXs into a focal adhesion. Continuing application of force to focal adhesions results in the formation of fibrillar adhesions and reorganization of the extracellular matrix. The formation of these adhesions depends on actomyosin contractility and matrix pliability.

Actomyosin↗

Nodular amyloidosis treated with a pulsed dye laser.

BACKGROUND: Nodular amyloidosis is a rare form of primary localized cutaneous amyloidosis which is characterized by single or multiple nodules located on the extremities, trunk, genitalia, or face. OBJECTIVE: To determine the clinical and histologic response of nodular amyloidosis to pulsed dye laser treatment. METHODS: Biopsy-proven amyloid nodules were treated with a 585-nm pulsed dye laser (average fluence 5.25 J/cm2; 10 mm spot) at 6- to 8-week time intervals. Clinical and histologic examination of laser-irradiated nodules were performed before and 6 weeks after the final laser treatment. RESULTS: Clinical improvement in the color, size, and pliability of nodules was noted and maintained for 6 months. Histologic examination revealed decreased inflammation and improvement in dermal collagen after laser irradiation. CONCLUSIONS: Since amyloid fibrils may be formed in association with dermatan sulfate-an essential matrix component in collagen fiber formation, it is postulated that the improvement seen in amyloid nodules after pulsed dye laser treatment may be attributed to a mechanism similar to that seen with hypertrophic scars.

Amyloidosis↗

Use of a 585 nm pulsed dye laser for the treatment of morphea.

INTRODUCTION: The clinical presentation of morphea varies from localized plaques to generalized eruptions. Its cause remains unknown and medical treatments have often proved unsatisfactory. Studies have previously shown that improvement of hypertrophic scars and fibrotic skin can be achieved with the use of a 585 nm pulsed dye laser (PDL). METHODS: A case of plaque-type morphea was treated with 585 nm pulsed dye laser irradiation at an average fluence of 5.0 J/cm2 at bimonthly time intervals. RESULTS: Marked clinical improvement as evidenced by improved pliability and skin coloration was seen after 4 successive PDL treatments. No side effects or complications were encountered. CONCLUSION: Pulsed dye laser therapy is a viable treatment option for morphea. The mechanism of its effect in this condition remains unknown.

Adult↗

585-nm pulsed dye laser in the treatment of surgical scars starting on the suture removal day.

BACKGROUND: The optimal time frame to improve the quality and cosmetic appearance of scars by laser therapy has not been clearly elucidated by prior controlled clinical trials. OBJECTIVE: To determine the efficacy of the 585-nm pulsed dye laser (PDL) in the treatment of surgical scars starting on the day of suture removal. METHODS: Eleven patients (skin types I-IV) with 12 postoperative linear scars that were greater than 2 cm were treated three times on monthly intervals with the 585-nm PDL (450 micro s, 10-mm spot size, 3.5 J/cm2 with 10% overlap) on one scar half, whereas the other half received no treatment. Scars were later evaluated by a blinded examiner using the Vancouver Scar Scale (VSS) for pigmentation, vascularity, pliability, and height. Scars were then blindly examined for cosmetic appearance using a visual analog scale. RESULTS: One month after the last treatment, final scar analysis by the blinded examiner revealed a significant difference between treated and untreated sites, with the treated halves scoring better in all scar parameters in the VSS and in cosmetic appearance. The treated halves demonstrated an overall average improvement in the VSS between the first treatment score and the final score of 54% versus 10% in the controls (P=0.0002). The cosmetic appearance score (0=worst; 10=best) at final assessment was significantly better for the treated scars, scoring 7.3 versus the averaged control score of 5.2 (P=0.016). CONCLUSION: The 585-nm PDL is effective and safe in improving the quality and cosmetic appearance of surgical scars in skin types I-IV starting on the day of suture removal.

Adult↗

Ultrasound assessment of scald scars in Asian children receiving pressure garment therapy.

BACKGROUND/PURPOSE: Scar formation after scald injury in children is managed commonly by application of pressure garment. The duration of the treatment depends on clinical assessment. The objective of the study was to evaluate the efficacy of the pressure garment therapy and to correlate the clinical assessment scar thickness with ultrasound measurement. METHODS: Prospective study of children with hypertrophic scars receiving pressure garment therapy was carried out between 1993 and 1998. The scars were mapped with paper cutting. Representative scar sites were scored clinically according to Vancouver General Hospital Burn Assessment Scale (pigmentation, vascularity, pliability, height, pain, itchiness). Blind to the clinical results, 2 radiologists measured the scar thickness of the same sites with ultrasonography (5 to 10 MHz transducer, General Electric LOGIQ 500). The correlation between the clinical scores and ultrasound measurement of scar thickness were analysed using analysis of variance (ANOVA). RESULTS: The median age of 58 children with scald injury (M:F = 36:22) was 2.8 years (0.5 to 15.8 years). The mean percentage of scald was 8.8% (24% had scald area >10%). In addition, there were 5 children with burn from naked flame and 9 children with keloid secondary to surgical incisions. With pressure garment therapy, the scar thickness reached a plateau 1 (1/2) years after the injury and began to decline gradually thereafter. The clinical estimation of scar thickness correlated well with the ultrasound measurement (ANOVA, P =.003, Adjusted R square = 0.99). The accuracy rate of clinical thickness estimation was 67%. Overestimation and underestimation accounted for 15% and 18%, respectively. There also was good correlation between total clinical score and the ultrasound measurement (ANOVA, P <.001). CONCLUSIONS: In children with scald injury, the pressure garment needs to be continued for a minimum of 1 year. Ultrasound measurement correlates well with overall score of the Vancouver General Hospital Burn Assessment Scale but not individual components of the scoring system except wound height.

Adolescent↗

Hydroxyapatite cement: an alternative for craniofacial skeletal contour refinements.

Hydroxyapatite cement is a calcium phosphate preparation composed of tetracalcium phosphate and dicalcium phosphate anhydrous powders. When mixed with water it isothermically forms a paste which can easily be shaped intraoperatively. This mixture sets in approximately 15-20 min and converts into water insoluble, nonceramic, microporous hydroxyapatite in 4 h. This biomaterial was used to correct either congenital or traumatic craniofacial contour irregularities or deficiencies in 20 patients. On follow-up there was only one patient who required repeat surgery for further correction, there were no other complications. The good cosmetic results, the ease of operation, the pliability of the cement paste allowing precise moulding during application, the short operation time and the avoidance of a donor site makes hydroxyapatite cement an attractive material for treating craniofacial contour defects. Careful long term follow-up is necessary to establish its safety and reliability.

Adolescent↗

Reconstructive surgery using an artificial dermis (Integra): results with 39 grafts.

Integra was initially developed for the primary coverage of acute burns. It acts as a network for dermal reconstruction. An epidermal graft overlay is necessary after 3 weeks to achieve the in vivo reconstruction of a full-thickness skin equivalent. The quality of the functional and aesthetic results achieved led us to evaluate the potential of Integra in the treatment of burn scars and for general reconstructive surgery. We present a series of 31 patients who underwent Integra grafting for reconstructive surgery at a total of 39 operational sites. The average area grafted per procedure was 267 cm(2). Complications (silicone detachment, failure of the graft, haematoma) were observed in nine cases. The length of follow-up ranged from 0.5 year to 4 years. Two patients (two sites) were lost to follow-up; the final results in the remaining patients were considered to be good in 28 cases, average in six cases and poor in three cases. The disadvantages of using Integra in reconstructive surgery are the necessity of two operations, the risks of infection under the silicone layer, of the silicone becoming detached and of recurrence of contraction. On the other hand, Integra has many advantages including its immediate availability, the availability of large quantities, the simplicity and reliability of the technique, and the pliability and the cosmetic appearance of the resulting cover. In the light of these preliminary results, Integra appears as a new alternative to full-thickness skin grafting, skin expansion and even skin flaps for reconstructive surgery.

Adolescent↗