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PubMed1992

Function of DR-positive thyrocytes from patients with Graves' disease: quantitative analysis of thyroid peroxidase content by fluorescent photometry.

A considerable number of thyrocytes in patients with autoimmune thyroiditis ectopically express HLA-DR antigen. Furthermore, it has been reported that interferon-gamma-induced DR-positive thyrocytes in vitro secrete less thyroid hormone in response to TSH stimulation compared with DR-negative ones. However, the function of the intrinsically DR-positive thyrocytes is unknown. To evaluate their function, we stained by immunofluorescence for both DR antigen and thyroid peroxidase (TPO) in thyroid epithelial cells from patients with Graves' disease. We also measured the quantity of DR antigen and TPO using fluorescent photometry. The content of TPO was not significantly reduced in DR-positive thyrocytes compared with that in DR-negative thyrocytes. The TPO content is one measure of thyrocyte function. There was no significant difference between DR-positive and DR-negative thyrocytes. In conclusion, the function of DR-positive thyrocytes in vivo was not suppressed compared with that of DR-negative thyrocytes.

Adult↗

PubMed2003

Classical photometry of prefractal surfaces.

Using the scale invariance of classical photometry, we develop an approach to finding the photometric function of prefractal structures that form a random topography. The photometric function of the prefractal surfaces is found as the general solution of the resulting differential equation in partial derivatives. The function depends on two parameters: the number of hierarchical levels of the prefractal structures and the roughness parameter of the single-level generation. As a limiting case, the approach includes our previous theory that considered fractoids.

Journal Article↗

PubMed2000

Comparisons of spore dosimetry and spectral photometry of solar-UV radiation at four sites in Japan and Europe.

In order to develop monitoring and assessment systems of biologically effective doses of solar-UV radiation, concurrent measurements of spectral photometry and spore dosimetry were conducted in summer months at four sites in Japan and Europe. Effectiveness spectra were derived by multiplying spectral irradiance in 0.5 nm steps between 290 and 400 nm with the inactivation efficiency of the spores determined using monochromatic radiation of fine wavelength resolution. Shapes of the effectiveness spectra were very similar at the four sites exhibiting major peaks at 303.5, 305.0, 307.5 and 311.0 nm. The dose rates for spore inactivation from direct survival measurements and from calculations by the integration of the effectiveness spectra were compared for 174 data points. The ratios (observed/calculated) of the two values were concordant with a mean of 1.26 (+/- 0.24 standard deviation [SD]). The possible causes for the variations and slightly larger observed values are discussed.

Bacillus subtilis↗

PubMed1987

[An automated unit for manual photometry and morphometry].

An automated unit for manually controlled photometry and morphometry is described. It enables the operator to carry out photometrical measurements according to the plug-method and morphometrical measurements with the help of an object-micrometer or some kind of a grid. The data obtained are stored in the "Elektronika D3-28" microcomputer to be statistically evaluated after the end of sampling. New variables can be obtained defined by the user as a product of some source variables or their inverses. The data and control structures of the system are described in detail.

Cytophotometry↗

PubMed1979

[Determination of Fe, Cu and Zn by a mechanised micro-method of flame photometry (author's transl)].

By sample application in measured quantities ("injection method") the quality of serum required for the determination of trace elements can be considerably decreased with essentially no loss in sensitivity. The volume of solution, 50 or 100 microliters, required for a single measurement is added to the nebulizer of the AAS apparatus manually with a bulb pipette, or automatically by a suitable sample changing system. A total of 500 microliters serum is sufficient for the determination of traces of Fe, Cu, and Zn. It was shown that under suitable conditions a total volume of 25 microliters serum is sufficient for the determination of Cu and Zn by flame atomic absorption-atomic emission photometry.

Copper↗

PubMed1963