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PubMed1979

Effect of organic matter supplementation on nitrogen transformations in soils. II. Nitrogen balance and yield.

Under controlled laboratory conditions, the effect of the application of uncomposted plant materials (sawdust and maize stalks) or horns and hooves (narrow C/N ratio) on soil nitrogen and plant growth was investigated. From nitrogen balance calculations it was found that the addition of the wide C/N ratio materials alone either increased soil nitrogen through N2-fixation or lessened nitrogen loss through denitrification. Although such materials resulted in the immobilisation of mineral nitrogen, no nitrogen starvation symptoms were noted on the growing plants. Negative nitrogen balances were usually obtained when soils received mineral nitrogen fertilizers either alone or together with maize stalks or supplemented with the narrow C/N ratio organic material.

Fertilizers↗
PubMed1997

Measurement of urinary urea nitrogen content as an estimate of the amount of total urinary nitrogen loss in dogs in intensive care units.

OBJECTIVE: To investigate whether urinary urea nitrogen (UUN) content can be used to accurately estimate total urinary nitrogen content in hospitalized dogs. DESIGN: Prospective, cohort study. ANIMALS: 24 client-owned dogs admitted to an intensive care unit of a veterinary teaching hospital. PROCEDURE: Indwelling urinary catheters and closed collection systems were placed for the purpose of monitoring urine output. Urine was collected during a 24-hour period from each dog. For each collection, the total volume was measured. Urine was analyzed for urea nitrogen content at an in-house laboratory, using standard methods for determination of BUN content. Total urinary nitrogen content was determined by another laboratory, using Kjeldahl's method. Correlation between UUN content and total urinary nitrogen content was evaluated by use of linear regression analysis. RESULTS: Total urinary nitrogen content adjusted for metabolic body weight ranged from 0.21 to 2.21 g/ kg0.75/d (mean +/- SD, 0.81 +/- 0.46 g/kg0.75/d). Adjusted UUN content ranged from 0.05 to 1.50 g/kg0.75/d (0.54 +/- 0.38 g/kg0.75/d). Total urinary nitrogen content was highly correlated with UUN content (R2, 0.88; P < 0.001). CLINICAL IMPLICATIONS: Measurement of UUN content can be used to estimate total urinary nitrogen content in a clinical setting. Furthermore, critical illness in dogs is associated with substantial catabolism of endogenous proteins.

Animals↗
PubMedDate not supplied

Nitrogen dioxide, the oxides of nitrogen, and infants' health symptoms. ALSPAC Study Team. Avon Longitudinal Study of Pregnancy and Childhood.

In this cross-sectional postal study, the authors measured nitrogen dioxide levels inside infants' bedrooms and outside their homes. During the 2-wk monitoring period, the authors investigated the association between nitrogen dioxide levels and 20 infant symptoms. The subjects were 1,200 women who had infants aged 3-12 mo. Median levels of indoor and outdoor nitrogen dioxide were 6.8 and 12.6 ppb, respectively. Environmental factors that were associated significantly with indoor levels were gas cooking, cigarette smoking, reported traffic levels, and presence of a kerosene heater; use of a cooker hood was associated negatively with indoor nitrogen dioxide levels. There was no evidence for any short-term significant association between prevalence of respiratory symptoms and nitrogen dioxide levels. Diarrhea, the only symptom associated significantly and positively with indoor nitrogen dioxide levels, had unadjusted and adjusted odds ratios of 1.48 (95% confidence interval: 1.13, 1.95) and 1.38 (1.11, 1.70), respectively. This association is discussed in terms of a proposed mechanism with nitric oxide. No association between a gas cooker in the home and diarrhea was found. The association between diarrhea and nitrogen dioxide level might have been a chance finding; the authors investigated 20 symptoms, and at least 1 was expected to be significant at the .05 level. The finding, however, was similar to that reported in a previous study in which a gas cooker was a proxy for nitrogen dioxide exposure.

Adult↗
PubMed2003

A model of dynamics of leaves and nitrogen in a plant canopy: an integration of canopy photosynthesis, leaf life span, and nitrogen use efficiency.

A model of dynamics of leaves and nitrogen is developed to predict the effect of environmental and ecophysiological factors on the structure and photosynthesis of a plant canopy. In the model, leaf area in the canopy increases by the production of new leaves, which is proportional to the canopy photosynthetic rate, with canopy nitrogen increasing with uptake of nitrogen from soil. Then the optimal leaf area index (LAI; leaf area per ground area) that maximizes canopy photosynthesis is calculated. If leaf area is produced in excess, old leaves are eliminated with their nitrogen as dead leaves. Consequently, a new canopy having an optimal LAI and an optimal amount of nitrogen is obtained. Repeating these processes gives canopy growth. The model provides predictions of optimal LAI, canopy photosynthetic rates, leaf life span, nitrogen use efficiency, and also the responses of these factors to changes in nitrogen and light availability. Canopies are predicted to have a larger LAI and a higher canopy photosynthetic rate at a steady state under higher nutrient and/or light availabilities. Effects of species characteristics, such as photosynthetic nitrogen use efficiency and leaf mass per area, are also evaluated. The model predicts many empirically observed patterns for ecophysiological traits across species.

Ecosystem↗
PubMed1976

Parenteral nutrition in the septic patient: nitrogen balance, limiting plasma amino acids, and calorie to nitrogen ratios.

A series of eight septic patients was provided varying levels of beef fibrin protein hydrolysate by central vein in the presence of adequate calories for evaluation of nitrogen retention under septic conditions. The mean nitrogen intake to achieve nitrogen equilibrium was 240 mg/kg of body wt per day. This represents a 40% increase over that required to produce nitrogen equilibrium in normal adults. The mean caloric intake of these patients was 43.3 kcal/kg of body wt per day. The calorie to nitrogen ratio based on the above intake was calculated to be 180:1. In order to utilize effectively calorie to nitrogen ratios in the nutritional care of patients, it is suggested that ratios be standardized using daily total coloric expenditures. Correcting the mean measured resting calorie expenditures of these patients for minimal daily activity, a caloric to nitrogen ratio of 138:1 was obtained. The plasma amino acid ratios in these septic patients confirm the finding that valine and phenylalnine are limiting amino acids in a beef fibrin hydrolysate at infusion levels below 240 mg of N/kg of body wt per day. Analysis of the urinary excretion of total nitrogen, urea, and amino acids in two patients suggests that 30 to 50% of the infused peptides of a beef fibrin hydrolysate are lost in the urine in these septic patients.

Abscess↗
PubMedDate not supplied

Urinary urea nitrogen: too insensitive for calculating nitrogen balance studies in surgical clinical nutrition.

Urinary urea nitrogen (UUN) has been used as an estimate of total urinary nitrogen (TUN) when calculating nitrogen output for nitrogen balance (NB) studies. UUN is assumed to constitute 80 to 90% of the total nitrogen output; when estimating TUN from UUN, UUN values are multiplied by 1.25 to correct for non-urea nitrogen components. In order to evaluate the validity of estimating total urinary nitrogen output from measured UUN in a clinical setting, 491 UUN:TUN paired studies were performed on 24-hour urine collections in general surgical/trauma patients who had measured TUN outputs ranging from 0.04 to 54.0 g/d. Assessment of 315 NB studies was done to compare NB values of those calculated by using UUN as an estimate of TUN with those calculated from measured TUN. Patients in both studies were subdivided into four stress categories, using TUN/day as the index: less than 5 g, 5-10 g, 10-15 g, and greater than 15 g. On average, 80 to 90% of TUN is represented by the UUN. However, in our patient population the variability ranged from 12 to 112%. If these UUN values are used as estimates for TUN in calculating NB, variations of up to 12 g/d would result. Application of the correction factor of 1.25 is not consistent in correcting for nonurea nitrogen components in this clinical setting. The use of actual rather than estimated TUN may be a more accurate and appropriate method than UUN when calculating NB.

Adolescent↗
PubMed2002

NitroGenius: a nitrogen decision support system. A game to develop the optimal policy to solve the Dutch nitrogen pollution problem.

A nitrogen decision support system in the form of a game (NitroGenius) was developed for the Second International Nitrogen Conference. The aims were to: i) improve understanding among scientists and policy makers about the complexity of nitrogen pollution problems in an area of intensive agricultural, industrial, and transportation activity (The Netherlands); and ii) search for optimal policy solutions to prevent pollution effects at lowest economic and social costs. NitroGenius includes a model of nitrogen flows at relevant spatial and temporal scales including emissions of ammonia and nitrogen oxides and contamination of surface- and groundwaters. NitroGenius also includes an economic model describing relationships for important sectors and impacts of different nitrogen control measures on Gross Domestic Product (GDP), unemployment, energy use, and environmental costs. About 50 teams played NitroGenius during the Second International Nitrogen Conference. The results show that careful planning and selection of abatement options can solve Dutch nitrogen problems at reasonable cost.

Cost Control↗
PubMed1975

Relationship between ruminal ammonia and nonprotein nitrogen utilization by ruminants. II. Application of published evidence to the development of theoretical model for predicting nonprotein nitrogen utilization.

Results from published experiments dealing with several aspects of nitrogen utilization by ruminants were used to test the concept of zero utilization of nonprotein nitrogen under conditions where more ammonia (greater than 5 mg ammonia nitrogen/100 ml) is in the rumen than can be converted to microbial protein. Results from experiments where the flow of non-ammonia nitrogen to the abomasum of sheep was measured indicate that when urea was the source of supplemental nitrogen, a constant quantity of amino acids reached the abomasum for all rations ranging from 10 to 23% crude protein. From growth studies, addition of nonprotein nitrogen to low protein, high energy rations caused an improved rate of gain. Additions of nonprotein nitrogen to rations resulting in predicted ruminal ammonia concentrations greater than 5 mg ammonia nitrogen/100 ml rumen fluid were without benefit. From lactation studies, nonprotein nitrogen supplementation did not improve milk production if the ration contained more than 12.5% crude protein prior to supplementation or if the predicted ruminal ammonia concentration was greater than 4 mg ammonia nitrogen/100 ml rumen fluid. The importance of the amino acid requirement of the animal as well as the composition of the ration in designing and evaluating nitrogen supplementation studies is discussed.

Ammonia↗
PubMed2004

[Influence of water deficit and supplemental irrigation on nitrogen uptake by winter wheat and nitrogen residual in soil].

Pot experiment in greenhouse showed that water deficit at all growth stages and supplemental irrigation at tillering stage significantly decreased the nitrogen uptake by winter wheat and increased the mineral N residual (79.8-113.7 mg x kg(-1)) in soil. Supplemental irrigation at over-wintering, jointing or filling stage significantly increased the nitrogen uptake by plant and decreased the nitrogen residual (47.2-60.3 mg x kg(-1)) in soil. But, the increase of nitrogen uptake caused by supplemental irrigation did not always mean a high magnitude of efficient use of nitrogen by plants. Supplemental irrigation at over-wintering stage didn't induce any significant change in nitrogen content of grain, irrigation at filling stage increased the nitrogen content by 20.9%, and doing this at jointing stage decreased the nitrogen content by 19.6%, as compared to the control.

Absorption↗
PubMed2005

Assessment of nitrogen excess in an agricultural area using a nitrogen balance approach.

A pilot study has been initiated to develop an approach for quantification of nitrogen excesses from agricultural activities that involve greenhouse farming in Kumluca Plain, Turkey. Detailed calculations utilizing the nitrogen balance method (NBM) were carried out at nine different locations within the plain over a time period of one year. The major contributing factors and governing operative mechanisms taken into consideration were nitrogen application rates both as organic and chemical fertilizers, irrigation water application practices, and nitrogen uptake by plants. The adopted approach yielded valuable information such as plant nitrogen uptake efficiencies, excess nitrogen, leaching rates and leachate nitrogen concentrations. Further, a site specific multiple linear regression model has been developed to estimate the ratio (N(leachate)/N(groundwater)) as a function of independent variables: farming age, excess nitrogen application and SEEPAGE Index Number. The negative sign of the model parameters implies that the ratio (N(leachate)/N(groundwater)) decreases as values of the independent variables increase. The adopted approach and the obtained results can beneficially be applied to similar sites to establish basic parameters of irrigation and fertilizer application operations.

Conservation of Natural Resources↗
PubMed1984

Effect of dietary nitrogen on urinary excretion of non-protein nitrogen in adolescent sickle cell patients.

The nitrogen economy of sickle cell patients has been found to be FRpaired due to several factors including poor absorption of protein products and higher than normal urinary nitrogen excretion. The increased urinary loss of nitrogen is probably due to increased protein catabolism as a result of inadequate intestinal supply of nitrogen and its poor utilization. The relationship between urinary nitrogen excretion and dietary nitrogen intake was investigated. The results confirmed that there was higher than normal nitrogen excretion in the sicklers at all levels of nitrogen intake. Creatinine excretion, expectedly, was lower in the sicklers due to their smaller physical stature. Uric acid excretion was higher in the sicklers, and so was the mean urine acid to creatinine ratio (0.64 in the sicklers and 0.34 in the normals). The ratio was not affected by the diet, indicating that there was increased purine metabolism in the sicklers.

Adolescent↗
PubMed2005

Predicted high-energy molecules: helical all-nitrogen and helical nitrogen-rich ring clusters.

Helical all-nitrogen and nitrogen-rich ring clusters, new types of potential high-energy molecules, were investigated in the computational study reported here. Stable helical all-nitrogen clusters N26 and N46 and nitrogen-rich helical structure N26H16 formed by fused six-membered rings were found and characterized as proper energy minima by having real frequencies for all eigenvectors of the Hessian matrix. Furthermore, the stability of [6] N-ring helix was studied by calculating the barrier of dissociation reaction. The potential of these type molecules as high-energy density materials was studied. For a better intuitive understanding of the unusual bonding patterns, the molecular isodensity contour (MIDCO) surfaces for [6] N-ring helix and [6] N-helicene were compared at some characteristic density threshold values of 0.20, 0.32, and 0.35 au. As indicated at these threshold values of the isodensity surfaces, the bonds of all-nitrogen clusters appear stronger than those of nitrogen-rich clusters. Apparently, the nitrogen-rich clusters are of higher energy than the all-nitrogen structures, especially if one takes into account the energy balance of bonds involving hydrogen.

Journal Article↗
PubMed1992

Effects of Nitrate and Ammonium on Gene Expression of Phosphoenolpyruvate Carboxylase and Nitrogen Metabolism in Maize Leaf Tissue during Recovery from Nitrogen Stress.

We previously showed that the selective accumulation of phosphoenolpyruvate carboxylase (PEPC) in photosynthetically maturing maize (Zea mays L.) leaf cells induced by nitrate supply to nitrogen-starved plants was primarily a consequence of the level of its mRNA (B Sugiharto, K Miyata, H Nakamoto, H Sasakawa, T Sugiyama [1990] Plant Physiol 92: 963-969). To determine the specificity of inorganic nitrogen sources for the regulation of PEPC gene expression, nitrate (16 millimolar) or ammonium (6 millimolar) was supplied to plants grown previously in low nitrate (0.8 millimolar), and changes in the level of PEPC and its mRNA were measured in the basal region of the youngest, fully developed leaves of plants during recovery from nitrogen stress. The exogenous supply of nitrogen selectively increased the levels of protein and mRNA for PEPC. This increase was more pronounced in plants supplemented with ammonium than with nitrate. The accumulation of PEPC during nitrogen recovery increased in parallel with the increase in the activity of glutamine synthetase and/or ferredoxin-dependent glutamate synthase. Among the major amino acids, glutamine was the most influenced during recovery, and its level increased in parallel with the steady-state level of PEPC mRNA for 7 hours after nitrogen supply. The administration of glutamine (12 millimolar) to nitrogen-starved plants increased the steady-state level of PEPC mRNA 7 hours after administration, whereas 12 millimolar glutamate decreased the level of PEPC mRNA. The results indicate that glutamine and/or its metabolite(s) can be a positive control on the nitrogen-dependent regulation of PEPC gene expression in maize leaf cells.

Journal Article↗
PubMed1996

Salvage of exogenous urea nitrogen enhances nitrogen balance in normal men consuming marginally inadequate protein diets.

1. Urea kinetics were measured in six healthy men using prime/intermittent oral doses of [15N15N] urea, after five days consuming one of four diets which varied in their nitrogen content: a reference diet (REF, 70 g of protein and 11.2 g of N); a low-protein diet (LP, 30 g of protein and 4.8 g of N); a low-protein diet with 6.9 g of urea added (LP-U1, 30 g of protein and 8 g of N); a low-protein diet with 13.7 g of urea added (LP-U2, 30 g of protein and 11.2 g of N). 2. Apparent nitrogen balance on the REF diet was significantly better than on the LP or the LP-U1 diets. The addition of the higher level of urea in the LP-U2 diet enhanced apparent nitrogen balance compared with the LP or LP-U1 diets, and was not different to apparent nitrogen balance on the REF diet. 3. On the LP, LP-U1 and LP-U2 diets, the rate of endogenous urea production was not different, and was about 60% of that on the REF diet, a statistically significant difference. The addition of a dietary supplement of urea increased the rate of urea appearance in the urea pool in direct relation to the dose of urea taken. There was no difference in the rate of appearance between the REF and LP-U2 diets, for both of which the rate of appearance was significantly greater than on the LP diet. 4. The excretion of urea in urine on the LP diet was 62% of that on the REF diet, a significant difference. There was no significant difference in the rate of urea excretion between the REF, LP-U1 and LP-U2 diets. 5. The rate of urea hydrolysis by the colonic microflora on the REF diet was more than twice that on the LP or LP-U1 diets. Supplementation with urea at the higher level, LP-U2, significantly increased hydrolysis to the same level as on the REF diet. Most of the nitrogen derived from urea hydrolysis was retained in the metabolic pool (> 80%), with no difference in the rate of retention between the REF and LP-U2 diets, both greater than the LP or LP-U1 diets. 6. The dietary supplements of urea increased the size of the body urea pool significantly. Renal clearance of urea was highest on the REF diet and decreased 13-29% on the low-protein diets. Bowel clearance was highest on the REF diet and decreased 46-55% on the low-protein diets. Neither urinary excretion of urea nor urea hydrolysis in the bowel were related simply to the concentration of urea in blood. Urea hydrolysis related most closely to the rate of appearance of urea in the urea pool. 7. The salvage of urea nitrogen was increased on the highest level of supplementation, but the overall sensitivity of the system was low, suggesting that other factors might be limiting for effective urea hydrolysis and the salvage of urea nitrogen.

Adult↗
PubMed2000

Nitrogen regulatory protein C-controlled genes of Escherichia coli: scavenging as a defense against nitrogen limitation.

Nitrogen regulatory protein C (NtrC) of enteric bacteria activates transcription of genes/operons whose products minimize the slowing of growth under nitrogen-limiting conditions. To reveal the NtrC regulon of Escherichia coli we compared mRNA levels in a mutant strain that overexpresses NtrC-activated genes [glnL(Up)] to those in a strain with an ntrC (glnG) null allele by using DNA microarrays. Both strains could be grown under conditions of nitrogen excess. Thus, we could avoid differences in gene expression caused by slow growth or nitrogen limitation per se. Rearranging the spot images from microarrays in genome order allowed us to detect all of the operons known to be under NtrC control and facilitated detection of a number of new ones. Many of these operons encode transport systems for nitrogen-containing compounds, including compounds recycled during cell-wall synthesis, and hence scavenging appears to be a primary response to nitrogen limitation. In all, approximately 2% of the E. coli genome appears to be under NtrC control, although transcription of some operons depends on the nitrogen assimilation control protein, which serves as an adapter between NtrC and final sigma(70)-dependent promoters.

Artificial Gene Fusion↗
PubMedDate not supplied

[Functional activity of exoglycans from Rhizobium leguminosarum bv. viciae 250a and its nitrogen-resistant mutant M-71 during the formation of legume-rhizobia symbiosis against a high-nitrogen background].

The functional activity of the exoglycan complex (EGC) polysaccharides from Rhizobium leguminosarum bv. viciae 250a and its nitrogen-resistant mutant M-71 capable of inducing the formation of nitrogen-fixing nodules on pea roots against a high-nitrogen background (4.8 mM NO3-) was studied in vegetation tests. For this purpose, the bacterial inoculum washed free of its own exoglycans was supplemented with EGC of this or another strain grown in the presence of 6 or 20 mM nitrate. The best symbiotic characteristics (nodule number and nitrogenase activity, mass of the roots and aerial parts of plants) were recorded when the inoculum cells and exoglycans were obtained from strain M-71 grown in the presence of 20 mM nitrate. When the plants were inoculated with the cells (grown at 6 mM nitrate) + EGC (obtained at 6 mM nitrate) of this strain, the nodulation characteristics and the effectiveness of symbiosis decreased 1.5-2-fold. Partial recovery of the symbiotic potential of strain M-71 was observed when EGC (obtained at 20 mM nitrate) was substituted for its exoglycans (obtained at 6 mM nitrate). In the presence of exoglycans of the parent strain 250a (obtained at 6 or 20 mM nitrate), the mutant formed a substantially lesser number of nodules with a very low nitrogen-fixing activity. In turn, the mutant exoglycans synthesized in medium with either high or low nitrate nitrogen concentration did not recover the fix+ phenotype of strain 250a capable of forming symbiosis with pea plants only against a low-nitrogen background. When studying the relative content of high-molecular-weight exopolysaccharide components and low-molecular-weight glycans in the exoglycan complex, it was established that, in strain 250a (grown at 6 and 20 mM nitrate), as well as in its mutant M-71 (grown at 6 mM nitrate), exopolysaccharides prevailed, accounting for 72-75% of the sum of both types of glycopolymers, while low-molecular-weight glycans accounted for 25-28%. In contrast, in the EGC of strain M-71 obtained at 20 mM nitrate, which was the most active inducer of the formation of the symbiotrophic system by strain M-71 in the presence of a high mineral nitrogen concentration, low-molecular-weight glycans were the main component, accounting for 61% of total glycopolymers, while the polysaccharide content was 39%. Low-molecular-weight exoglycans are supposed to be involved in maintaining the physiological activity and the symbiotic status of rhizobia under unfavorable environmental conditions.

Culture Media↗
PubMedDate not supplied

Intensity of nitrogen fixation in yellow lupine in presence of different doses of mineral nitrogen.

The effect of different doses of mineral nitrogen on the nitrogen-fixing activity of lupine nodules during vegetation was studied. It was shown that 0.5 of the dose of mineral nitrogen had an inhibitory effect only in the initial period of development of the nodules, whereas full and double doses of mineral nitrogen inhibited nitrogen fixation throughout the period of vegetation. Supplements of mineral nitrogen switch the lupine nutrition from the symbiotic to the autotrophic type, which is accompanied by a decrease in the nitrogen-fixing activity in the nodules and by an increase in the nitrate-reducing capacity of the roots and leaves.

Fabaceae↗
PubMed2006

Varying stable nitrogen isotope ratios of different coastal marsh plants and their relationships with wastewater nitrogen and land use in New England, USA.

The stable nitrogen isotope ratios of some biota have been used as indicators of sources of anthropogenic nitrogen. In this study the relationships of the stable nitrogen isotope ratios of marsh plants, Iva frutescens (L.), Phragmites australis (Cav.) Trin ex Steud, Spartina patens (Ait.) Muhl, Spartina alterniflora Loisel, Ulva lactuca (L.), and Enteromorpha intestinalis (L.) with wastewater nitrogen and land development in New England are described. Five of the six plant species (all but U. lactuca) showed significant relationships of increasing delta (15)N values with increasing wastewater nitrogen. There was a significant (P < 0.0001) downward shift in the delta (15)N of S. patens (6.0 +/- 0.48 per thousand) which is mycorrhizal compared with S. alterniflora (8.5 +/- 0.41 per thousand). The downward shift in delta (15)N may be caused by the assimilation of fixed nitrogen in the roots of S. patens. P. australis within sites had wide ranges of delta (15)N values, evidently influenced by the type of shoreline development or buffer at the upland border. In residential areas, the presence of a vegetated buffer (n = 24 locations) significantly (P < 0.001) reduced the delta (15)N (mean = 7.4 +/- 0.43 per thousand) of the P. australis compared to stands where there was no buffer (mean = 10.9 +/- 1.0 per thousand; n = 15). Among the plant species, I. frutescens located near the upland border showed the most significant (R (2) = 0.64; P = 0.006) inverse relationship with the percent agricultural land in the watershed. The delta (15)N of P. australis and I. frustescens is apparently an indicator of local inputs near the upland border, while the delta (15)N of Spartina relates with the integrated, watershed-sea nitrogen inputs.

Ecosystem↗