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[Preliminary and multidisciplinary study of three Coptic mummies of the Testut-Latarjet museum at Lyon].

In 1901 Albert Gayet raked up from Antinoe three mummies exhibited to day at the Anatomy Museum of Lyon. The study of the three mummies was made in detail as to their dress, anthropometric and scannographic findings. The clothes were characteristic of coptic civilization. The radiographic date gave a life span of around 40 years. The X-ray imagery shows the remains of cerebral and visceral organs. The sexual criteria are thought to be those of two women and undetermined for the child. Later, several investigations like endoscopic autopsy, tooth microscopy and chromosomic map will be necessary.

Anthropometry↗

[Stone age deerfly (Lipoptena cervi) found with a mummy in a glacier].

In hair samples from the accompanying equipment of a human mummy preserved in glacial ice in South Tyrol for more than 5000 years, numerous remains of insects were found. These fragments corresponded in form, size and structural organization to the features of the deer ked, Lipoptena cervi. Considering the biology and ecology of L. cervi with regard to the finding place of the mummy at an altitude of 3210 m above sea level in a glacier it is concluded that the deer keds invaded the equipment before and not after the death of the Stone Age man.

Animals↗

An examination of the dental state of an Egyptian mummy by means of computer tomography: a contribution to 'dentistry in Ancient Egypt'.

In 1991 mummies from Leipzig collections were investigated by means of conventional X-ray techniques and by computer tomography. The dental state of one of these mummies prompted a re-examination of the question of whether a dental profession actually existed in Ancient Egypt or not. To answer this question, different types of source material are discussed.

Egypt, Ancient↗

[Paleopathology of ancient Egyptian mummies and skeletons. Investigations on the occurrence and frequency of specific diseases during various time periods in the necropolis of Thebes-West].

The scientific investigation of mummies and skeletons provides considerable data for the reconstruction of the living conditions and diseases of past populations. We describe the data on four completely analyzed tomb complexes from the huge necropolis of Thebes-West in Upper Egypt dating to different time periods. A total of 211 individuals from the so-called "Middle Kingdom" (MK, c. 2050-1750 BC) were compared to 273 individuals from the "New Kingdom" (NK) to "Late Period" (LP, in total 1550-500 BC). The age at death and the sex ratio were comparable between both groups. There was a high rate of early death with a maximum between the 2nd and 3rd decade of life but infant/adolescent burials were comparably rare. This early death is assumed to be due to an elevated prevalence of various infectious diseases. Likewise, a high rate of tuberculosis infections was seen in those individuals regardless of which time period they came from. Metabolic disorders with osseous manifestations, such as scurvy, osteomalacia and chronic anemia (cribra orbitalia, porotic hyperostosis) were found with a high frequency in the MK populations but significantly less in the NK-LP populations. On the other hand signs of trauma were comparably high, and lesions due to degenerative joint and vertebral diseases were significantly higher in LP than in MK or NK individuals suggesting a higher mechanical load in the later populations. Cases of malignant (secondary) bone tumors and various soft tissue/organ diseases indicate that "civilization" disorders were present when the living conditions assured survival into advanced age. In summary, we provide circumstantial evidence that the systematic and concise analysis of mummy and skeletal remains can allow a reconstruction of major aspects of life and disease in historic populations, although a complete reconstruction is not possible.

Bone Diseases↗

"Mummies and babies" and friends and lovers in Lesotho.

This paper examines an institutionalized friendship among adolescent girls and young women in southern Africa. Lesotho's economy is based on migrant male labor which leaves the women dependent on male earnings or subsistence from the land, and also creates unstable marital relations. Young girls in the modern schools develop close relationships, called "mummy-baby," with slightly older girls. Sexual intimacy is an important aspect of these relationships. Mummy-baby relationships not only provide emotional support prior to marriage, but also a network of support for married and unmarried women in new towns or schools, either replacing or accompanying heterosexual bonds.

Adolescent↗

Mummies that I have known. A pediatrician's venture in the field of paleopathology.

We describe the activities of a pediatrician in the field of medical archeology. Having pioneered the use of electron microscopy in the examination of Egyptian mummified tissue, we also describe some of the exciting pathological findings from various autopsies of ancient Egyptian mummies. These findings give us a glimpse of disease and death in ancient Egypt and of the evolution of these diseases as they affect man today.

Adolescent↗

Goiter in an eighteenth-century Sicilian mummy.

Goiter is still a frequent pathological condition of the thyroid gland. Goiter consists of an enlargement of the gland caused by several physiopathologic events, the most important of which is deficient intake of iodine. A series of eighteenth-century mummies housed in the church of Santa Maria della Grazia in Comiso includes one individual showing a pathological condition, with a very large swelling in the antero-inferior region of the neck. X-rays showed small scattered radiopaque foci. Routine histology showed a large number of circular follicles immersed in fibrous tissue. Finally, immunohistochemistry revealed a strong reactivity for thyroglobulin. The data confirmed the nature of the tissue as thyroid, and the macroscopic diagnosis is that of thyroid goiter.

Goiter↗

ABO blood groups in Peruvian mummies. I. An evaluation of techniques.

Blood groups of Peruvian mummies of known origin were determined by three different methods: agglutination-inhibition, induction of antibody production and mixed cell agglutination. The three techniques gave identical results, but the last two were useful in establishing the presence of H (O) antigen, while the first technique would not. The results indicate the presence of A, B, AB and O blood groups in America prior to known European contact. This suggests the need for a revision of concepts of blood groups in the American Indian.

ABO Blood-Group System↗

Seven Chinchorro mummies and the prehistory of northern Chile.

The mummification methods of an ancient maritime population on the northern coast of Chile are reviewed and the findings in an additional seven individuals are reported. Members of this cultural group, Chinchorro, practiced a selective, elaborate form of artificial mummification which persisted more than 4,000 years. Its complexity diminished with time, gradually disappearing after 2,000 B.C. One of the seven individuals herein reported is a rather poorly but spontaneously ("naturally") preserved body that may represent the oldest mummy reported to date--about 9,000 years old. Chemical reconstruction of their diet demonstrates that the principal component was derived from marine resources with only minor supplementation from terrestrial hunting as well as food gathering from river mouth vegetal sources, confirming the marine dependence of their adaptational strategy.

Adult↗

Temporal trends in stable isotopes for Nubian mummy tissues.

From Meroitic to Christian times (350 B.C.-A.D. 1400), Sudanese Nubia experienced political, economic, cultural, and environmental upheaval. Change in any one of these aspects of ancient lifeways can affect subsistence. Dietary patterns from this period are reconstructed by measuring stable carbon and nitrogen isotope ratios in tissue samples from 146 mummies excavated from five sites in the Wadi Halfa area. On average, delta 13C values of bone collagen, muscle, and skin indicate high consumption levels of C3 plants (presumably wheat or barley staples, mixed vegetables, and fruits) throughout the sequence. However, during the X-Group period (A.D. 350-550), there is a statistically significant increase in consumption of C4 plants (millet or sorghum), which are predominant in both the archeological record and in modern crop production for most of the Northern Sudan. The X-Group period was also associated with a low Nile and political and economic restructuring. Increased use of C4 plants on a seasonal basis is also indicated by shifting delta 13C values along hair shafts for both X-Group and Christian periods. delta 15N values suggest that the major source of protein for all time periods came from herbivorous animals. A small, but significant increase in 15N over the 1,000-year sequence could be the result of fertilization.

Adult↗

Preservation of elastic system fibers and of collagen molecular arrangement and stainability in an Egyptian mummy.

The present findings show that both elastic system fibers and collagen markedly resisted change in tissues more than 2000 years old. The distribution of elastic fibers and elastic-related fibers (namely, oxytalan and elaunin fibers) in mummified tissues coincided with the observations made on the modern human tissues used as controls. The collagenous structures present in tissue sections obtained from the Egyptian mummy studied took on a deeply red colour when stained in the Picrosirius solution indicating that, as well as in the fresh controls, the basic groups in the collagen molecules were available for reacting with the strongly acidic dye Sirius Red. When viewed with polarized light, the collagen in the same tissue sections displayed an increased birefringence, which shows that the collagen molecules in mummified tissues maintain the oriented disposition which is typical of the modern human tissues used as controls. The methods employed have proved to be useful for the delineation of the elastic system fibers and of the collagenous scaffolding, which may be used as valuable landmarks in the study of the histoarchitecture of organs that have undergone considerable distortion.

Collagen↗

Juvenile cirrhosis in a 16th century Italian mummy. Current technologies in pathology and ancient human tissues.

The natural headless mummy of a young man from the Basilica of Saint Domenico Maggiore in Naples (16th century) showed at autopsy a well-preserved fibrous liver with a nodular surface, suggesting a case of cirrhosis. Stereo and light microscope study confirmed this diagnosis. To identify the possible etiology of this cirrhosis, additional techniques currently used in pathology were performed. Hemochromatosis and alpha1-antitrypsin deficiency were investigated without results. Investigation regarding Wilson's disease gave positive results, since the use of rhodamine staining, which is specific to detect the presence of copper in tissues, resulted in red-brown grains at light microscopy. The positive rhodamine test was invalidated by atomic absorption spectroscopy (AAS), which revealed normal copper levels in the tissues. These negative results and the clear and diffuse macronodularity of the liver suggest a case of post-necrotic cirrhosis.

Humans↗

STR typing of ancient DNA extracted from hair shafts of Siberian mummies.

The aim of this study was to determine if ancient hair shafts could be suitable for nuclear DNA analysis and to develop an efficient and straightforward protocol for DNA extraction and STR typing of ancient specimens. The developed method was validated on modern and forensic samples and then successfully applied on ancient hairs collected from Siberian mummies dating from the 16th to the early 19th centuries. In parallel extractions including or excluding a washing step were performed at least two times for each sample in order to evaluate the influence on the quantity of nuclear DNA yielded and on the typing efficiency. Twelve ancient individuals were analyzed through our approach and full and reliable profiles were obtained for four of them. These profiles were validated by comparison with those obtained from bone and teeth DNA extracted from the same ancient specimens. The present study demonstrates that the washing step cannot be considered as deleterious for DNA retrieval since the same results were obtained by the two approaches. This finding challenges the hypothesis that recoverable nuclear DNA is only found on the outer surface of hair shafts and provides evidence that nuclear DNA can be successfully extracted from ancient hair shafts. The method described here constitutes a promising way for non-invasive investigations in ancient DNA analysis for precious or historical samples as well as forensic casework analyses.

Bone and Bones↗

Identification of phenolic acids and inositols in balms and tissues from an Egyptian mummy.

A number of samples taken from an Egyptian mummy (ca. 100 B.C.) from the Guimet Museum in Lyon have been analyzed by GC-MS. Derivatives of aromatic acids (hydroxyhydrocinnamic, vanillic, protocatechuic and gallic acids) and inositols (non-methylated and mono-O-methyl) have been found among the constituents of extracts prepared by methanolysis and trimethylsilylation. From the reported electron impact mass spectra, ion sets where proposed for a sensitive and selective profiling of these selected compounds by mass fragmentometry. The source of gallic acid and inositol was found to be a vegetable tannin, an ingredient which was not previously known to be used for mummification in ancient Egypt. The nature and abundance ratios of the detected inositols also appeared to be a promising criterion to further investigate the botanical source of the tannin employed.

Adult↗

Dental and skeletal findings on an ancient Egyptian mummy.

The dentofacial structures of an ancient Egyptian mummy were radiographically evaluated from available computer tomographic scans. The cephalometric measurements obtained were compared to those available on ancient Egyptian Pharaohs as well as to modern cephalometric standards for adult males. The measurements on "Lady" Udja were closely related to both sets of cephalometric standards. The dental findings include: noticeable generalized attrition of the dentition, extracted lower first molar, and impacted maxillary third molars.

Cephalometry↗

Otopathology of Egyptian mummy Pum II: final report.

Otoscopic examination of the ears of Egyptian mummy PUM II with the operating microscope revealed a small oval perforation of the right tympanic membrane in the posteroinferior quadrant. The smooth and concise margins suggested an etiology of middle ear infection. A method which allows decalcification of ancient specimens without disintegration of the eardrum and other soft tissues within the temporal bone was described. There was evidence of histological repair at the margins of the perforation consistent with sequela of otitis media. No other abnormalities were observed in the temporal bones of this individual. There was no evidence of otosclerosis bilaterally. To our knowledge, this is the first time that a pathological tympanic membrane perforation has been histologically documented in an ancient temporal bone.

Cochlea↗

The Birmingham mummy: the first torticollis in history.

The Birmingham Mummy is of a warrior, 25-35 years of age, from the Graeco-Roman period. He was struck from the front by an arrow, which penetrated the soft tissues of the neck to some depth and lodged in the right infratemporal fossa. It is suggested that the wound became infected and that muscle spasm induced the torticollis which was not fully corrected on mummification.

Egypt↗