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Insertion abnormality of bilateral pectoralis minimus.

Bilateral insertion abnormality of pectoralis minimus (sterno-costo-coracoidian muscle) muscle was examined. The variant muscle was lying under the pectoralis major muscle and was medial to the pectoralis minor muscle. This muscle started from the first costal cartilage to the manubrium sterni and ended in the upper surface of the shoulder joint on the right side. On the opposite side, it took origin from the second costal cartilage to the manubrium sterni and the second costochondral joint, afterwards became a tendinous structure and divided into two on the coracoid process. The thicker part ended on the upper surface of the articular capsule of the shoulder joint, the thinner part inserted on the lateral third of inferior part of clavicle and fascia of subclavius muscle.

Adult↗

Fracture separation of the medial clavicular epiphysis: ultrasonography findings.

INTRODUCTION: It is evident that ultrasound could be a reliable substitute for radiography in very specific cases like stress or undisplaced fractures. MATERIALS AND METHODS: Ultrasound was used to examine a physeal fracture of the sternal end of the clavicle in a 3-year-old boy. RESULTS: Results included separation and a longer distance between the manubrium and clavicle on the left. A hyperechogenic structure between the manubrium and clavicle was considered to be a metaphyseal fragment. The patient was checked again 3 weeks post-injury. Sonography revealed haematoma resorption as well as callus formation between the fragments and along the medial metadiaphysis of the clavicle. CONCLUSION: Despite the interesting information that could be derived from the ultrasonic investigation of the medial end of the clavicle in our patient, it is mandatory to compare and prove the interpretation of the findings with other conventional methods like X-rays or CT scan.

Bony Callus↗

Minimally invasive endoscopic approach to the cervicothoracic junction for vertebral metastases: report of two cases.

The anterior cervicothoracic junction is difficult to expose and many techniques have previously been described. Most of them require an extensile exposure, which can lead to significant morbidity. The aim of this study is to present a less invasive approach, allowing the same exposure on the spine as a larger one. The approach begins with the same incision as the Smith-Robinson technique: a blunt dissection of the posterior face of the manubrium is performed with the finger. An endoscope is inserted through 10-mm trocars, one above the manubrium and the second through the second rib space. The upper mediastinal space is exposed; the dissection is performed on the left side, between the esophagus and trachea medially, between the innominate vein and brachio-cephalic artery distally, and between the left common carotid and internal jugular vein laterally. The recurrent nerve must be protected. Two patients with spine metastases underwent this new approach. A strut graft was fixed anteriorly after decompression of the spinal cord. Levels T1-T3 can be well exposed through this approach, allowing complete vertebral body removal at level T1 or T2. After body removal, the posterior longitudinal ligament is well exposed, allowing complete release of the spinal cord. The use of the endoscope is the key to providing a good view of the spine without an extensile exposure. This new approach is technically feasible. The exposure is sufficient for vertebral body resection and reconstruction by strut graft. The procedure is less aggressive and painful than sternotomy.

Cervical Vertebrae↗

Thickness distribution of fresh eardrums of cat obtained with confocal microscopy.

The aim of this study was to measure the spatial thickness distribution of the cat tympanic membrane (TM), a very thin, virtually transparent and delicate biological membrane. Axial fluorescence images taken perpendicular through isolated TM were recorded for five different cats using confocal laser scanning microscopy. Thickness was measured on the cross-section of the membranes in the axial images. A correction for focal shift due to refractive-index mismatch was applied. Similar thickness distributions were obtained in all measured samples (n = 9). The pars tensa had a rather constant thickness in the central region between the annulus and manubrium. The thickness increased steeply toward the peripheral rim. Thickness was smallest in the inferior region, with values ranging between 5.5 and 9 microm in the central part and up to 50 microm near the annulus. More superiorly, thickness was slightly higher, up to 20 microm, between the annulus and manubrium. The anterior part was thicker than the posterior side. These findings are strongly different from a current value in the literature. Our data allow a more precise representation of the eardrum in mathematical models, which are a prerequisite for a better understanding of middle-ear mechanics. The optical sectioning technique of the confocal microscope did not result in any preparation artifacts and was therefore also used to quantify shrinkage due to preparation of histological sections of TMs.

Animals↗

A new entomopathogenic nematode, Steinernema robustispiculum n. sp. (Rhabditida: Steinernematidae), from Chumomray National Park in Vietnam.

Steinernema robustispiculum n. sp. (Rhabditida: Steinernematidae) was isolated from woodland in Chumomray National Park, Sason, Sathay, Kontum, Vietnam. Its morphology, morphometrics, cross-hybridisation and the ITS-rDNA sequence analysis revealed that S. robustispiculum clearly differs from other known Steinernema spp. As in the cases of S. intermedium (Poinar, 1985), S. robustispiculum has very robust spicules, but it can be distinguished by the longer tail of the infective juvenile, lower E%, shorter spicules, the shape of the spicules, the number of genital papillae in the caudal region and the presence of a mucron on the male tail. S. robustispiculum has a lateral field resembling that of S. sangi Phan, Nguyen & Moens, 2001, but can be distinguished by a higher E%, higher D%, smaller length to width ratio of the spicules and the morphology of both the spicule head (manubrium) and the dorsal lobe of the spicule. The morphometrics of infective juveniles of S. robustispiculum are similar to those of S. monticolum Stock, Choo & Kaya, 1997; these species can be distingusihed by the position of the excretory pore, the smaller length to width ratio of the spicules, and the length and morphology of the spicule head (manubrium). The phylogenetic relationships within Steinernema Travassos, 1927, including the newly sequenced Vietnamese species S. robustispiculum n. sp., S. loci Phan, Nguyen & Moens, 2001, S. thanhi Phan, Nguyen & Moens, 2001 and S. sangi, are presented based on analyses of the ITS-rDNA. The ITS RFLP profiles obtained from 17 different restriction enzymes are also presented.

Animals↗

A transsternal approach to the upper thoracic vertebrae.

An anterior surgical approach to the upper thoracic vertebrae has been described. A T-shaped skin incision is used, with the horizontal limb 1 cm above the clavicle, and the vertical limb extending in the midline over the body of the sternum. A portion of the manubrium sterni, as well as the medial third of the clavicle, is resected; the avacular tissue plane between the carotid sheath laterally and the trachea and esophagus medially is developed to reach the prevertebral space. After surgery, immediate fusion is performed using the clavicle and manubrium. This procedure is well tolerated, and was associated with minimal morbidity and no mortality in a series of seven patients.

Humans↗

Thickness of the gerbil tympanic membrane measured with confocal microscopy.

Thickness data for the gerbil tympanic membrane, an extremely thin biological membrane, are presented. Thickness measurements were performed on fresh material using fluorescence images taken perpendicular through the membrane with a commercial confocal microscope. Thickness varies strongly across the membrane. Similar thickness distributions in all samples (pars tensa n = 11; pars flaccida n = 3) were observed. The pars tensa has a rather constant thickness of about 7 microm in the central region curving as a horse shoe upwards around the manubrium. In the most superior parts of the pars tensa thickness becomes gradually twice as large. Thickness increases also steeply from the central region towards the edges (about 35 microm near the annulus and 20 microm near the manubrium). A pronounced, local thickening of about 30 microm is present close to the edge and extends as a ring along the entire annular periphery of the pars tensa. Overall, the pars flaccida is thicker than the pars tensa and has a rugged surface. Its central region has a mean thickness of about 24 microm with a mean variation of about 4 microm. The average thickness in the inferior region is slightly larger than in the superior region. The pars flaccida thickens steeply, up to about 80 microm, near the edges.

Animals↗

The Sine oculis/Six class family of homeobox genes in jellyfish with and without eyes: development and eye regeneration.

The development of visual organs is regulated in Bilateria by a network of genes where members of the Six and Pax gene families play a central role. To investigate the molecular aspects of eye evolution, we analyzed the structure and expression patterns of cognate members of the Six family genes in jellyfish (Cnidaria, Hydrozoa), representatives of a basal, non-bilaterian phylum where complex lens eyes with spherical lens, an epidermal cornea, and a retina appear for the first time in evolution. In the jellyfish Cladonema radiatum, a species with well-developed lens eyes in the tentacle bulbs, Six1/2-Cr and Six3/6-Cr, are expressed in the eye cup. Six4/5-Cr is mainly expressed in the manubrium, the feeding, and sex organ. All three Six genes are expressed in different subsets of epidermal nerve cells, possibly of the RFamide type which are part of a net connecting the different eyes with each other and the effector organs. Furthermore, expression is found in other tissues, notably in the striated muscle. During eye regeneration, expression of Six1/2-Cr and Six3/6-Cr is upregulated, but not of Six4/5-Cr. In Podocoryne carnea, a jellyfish without eyes, Six1/2-Pc and Six3/6-Pc are also expressed in the tentacle bulbs, Six1/2-Pc additionally in the manubrium and striated muscle, and Six3/6-Pc in the mechanosensory nematocytes of the tentacle. The conserved gene structure and expression patterns of all Cladonema Six genes suggest broad conservation of upstream regulatory mechanisms in eye development.

Animals↗

Lower sternal reinforcement improves the stability of sternal closure.

BACKGROUND: This study uses a mechanical testing system to evaluate three methods of sternal closure. METHODS: Twelve sternal replicas composed of a polyurethane foam bone analogue were divided in the midline and reapproximated using three stainless steel wire techniques: six simple wires (6S), six figure-of-eight wires (6F8), or seven simple wires (7S), which included an extra wire at the lower sternum. The closures were subjected to increasing lateral distraction from 0 to 400 Newtons (N) (1 N = 0.224 lbs), and motion was measured using transducers stationed across the manubrium, midsternum, and lower sternum. RESULTS: With each method of closure, the manubrium was the most stable, the lower sternum the least stable, and the midsternum intermediate between the other two. There were also differences between sternal closure methods, but only at the lower sternum. Less sternal distraction was measured with the 7S than the 6S and 6F8 methods, starting at 100 N (0.20 +/- 0.06 mm vs 0.48 +/- 0.19 and 0.39 +/- 0.10, p = 0.003), and progressively increasing until the study was stopped at 400 N (1.64 +/- 0.39 mm vs 4.92 +/- 1.73 and 5.1 +/- 1.43 mm, p = 0.003). CONCLUSIONS: These data show that the lower sternum is the site of greatest instability and that reinforcement of this area with an additional wire effectively stabilizes the closure. Figure-of-eight wires are not superior to simple wires.

Bone Wires↗

Coil embolization of a false aneurysm with aorto-cutaneous fistula after prosthetic graft replacement of the ascending aorta.

AIM: To report palliative embolization of a false aneurysm over the distal suture line of an ascending aorta graft replacement. MATERIAL AND METHOD: A 78-year-old male patient was admitted for increasing bleeding of a chronic Manubrium ulceration, 20 months after coronary artery bypass complicated by perioperative ascending aorta dissection requiring prosthetic graft replacement. One month later, he underwent epiploplasty for a mediastinitis followed by long-term antibiotic therapy. Five months later, he presented with a Manubrium ulceration of the sternotomy. Spiral computerized tomography (CT) and aortography revealed a 20 mm anterior peri-prosthetic false aneurysm with a wide neck. Advanced age, active mediastinitis and patient's objection led us to perform percutaneous occlusion according to the Moret remodeling technique while protecting the coils release with balloon catheter inflation. RESULTS: No post-operative complication was observed and at 1-year follow-up the patient was doing well with no recurrent bleeding. Magnetic resonance imaging (MRI) and spiral CT controls confirmed coils stability without any internal flow. CONCLUSION: Percutaneous coils embolization of a large false aneurysm in the ascending aorta can be a palliative treatment in a surgically unsuited patient.

Aged↗

[Skeletal changes in Down's syndrome. A correlation between radiological and cytogenetic findings (author's transl)].

One hundred and two patients with Down's syndrome aged one day to 17 years were examined radiologically, seven of these repeatedly. Films obtained included the chest in two planes, lateral spine, left hand and pelvis. The following features were noted; the number of paired ribs, ossification centres in the manubrium, height of lumbar vertebral bodies, shortening of the phalanges, bone age and calculation of the acetabula and ilial angle and ilial index. In all patients the chromosomes were examined in order to determine the cytogenetic type. The following features were found to be more common in Down's syndrome than in normals: abnormal ossification of the manubrium (33%), aplasia of the twelfth rib (18%), high lumbar vertebral bodies (50%), brachymesophalangia of the fifth ray (62%) and changes in skeletal maturation (acceleration or retardation in 48%). Changes in the pelvis were typical; with increasing age, the acetabular angle falls and the ilial angle increases. The most striking skeletal changes were found in 84 patients with trisomy 21.

Adolescent↗

Modified anterior approach to the cervicothoracic junction.

STUDY DESIGN: This study reports the experience with four patients regarding a modified anterior approach to the cervicothoracic junction. OBJECTIVES: This technique was evaluated with respect to extent of exposure, ease of technique, and postoperative morbidity. SUMMARY OF BACKGROUND DATA: Previously reported anterior approaches to the cervicothoracic junction have described either full sternotomy resection of the left sternoclavicular junction or osteotomy of the clavicle. A simplified approach was chosen using a partial sternotomy, which has not been described previously for approaches to the spine. METHODS: Four patients with metastatic disease, in the region of the cervicothoracic junction, required decompression and stabilization for palliation of symptoms. An anterior approach was required for decompression. A standard cervical approach was combined with a partial median sternotomy and transverse osteotomy through the synostosis between the manubrium and body of the sternum. In three patients, the left innominate vein was divided. Decompression and anterior stabilization were followed by posterior stabilization at an interval of 4 to 7 days. RESULTS: This procedure was simple to perform, requiring little additional operative time for opening or closure. It provided excellent exposure from C3-T4. There was no associated morbidity related to the division of the manubrium or innominate vein. CONCLUSION: Partial sternotomy combined with a standard cervical incision provides excellent exposure to the cervicothoracic junction from C3-T4. It is technically simple to perform and avoids the risk of injury to subclavian vessels inherent in resection of the clavicle or sternoclavicular junction. There is no additional morbidity associated with this approach.

Cervical Vertebrae↗

Thickness distribution of fresh and preserved human eardrums measured with confocal microscopy.

HYPOTHESIS: In this study, the thickness distribution of the fresh human eardrum was measured and possible thickness changes in successive stages of preservation and preparation were studied. METHODS: The thickness measurement was performed on axial fluorescence images taken perpendicularly through the membrane with a confocal microscope. The influence of fixation and preservation (in Cialit solution) on the thickness was also investigated. The same eardrum was prepared (decalcified, dehydrated, and stained) for histologic sectioning and the thickness was measured on the sections using conventional light microscopy. RESULTS: Similar thickness distributions in the measured samples (n = 3) were observed. The pars tensa has a rather constant thickness in a central region curving as a horseshoe upward around the manubrium. The membrane thickens slightly from the inferior to the superior side. The anterior region is thicker than the posterior region. In narrow bands along the manubrium, peripheral rim, and in the region inferior to the umbo, a much larger thickness in comparison with that in the central region was found. Mean thicknesses of approximately 40, 50, and 120 microm were observed in the central region of the studied eardrums, respectively. CONCLUSION: Whereas the thickness distribution of the human eardrums shows similar features, the absolute thickness seems to vary a lot from one specimen to another. There is no significant difference in thickness of the same membrane in fresh, fixed, or preserved condition. Thus, human eardrums may be safely preserved in fixative for later thickness measurements. The histologic preparation process, however, causes a significant location-dependent shrinkage.

Age Factors↗

Mapping of internal mammary vessels by multidetector computed tomography for parasternal transthoracic intervention guidance.

OBJECTIVE: To measure the distances between the internal mammary arteries (IMAs) and the sternum for determining a route for parasternal transthoracic interventions. METHODS: Coronal reconstructed multidetector computed tomography images of 202 patients were evaluated to measure the distances between the IMAs and sternum at 3 levels (manubrium, midsternal corpus, and distal sternal corpus). The arrangements (2 veins-single artery or single vein-single artery) were documented. RESULTS: The most frequently encountered arrangement (42.1%) was a single vein and single artery on both sides. Mean distances on the right and left sides were 10.2 and 10.1 cm at the manubrium, 14.5 and 14.3 cm at the midsternal corpus, and 17.9 and 17.5 cm at distal sternal corpus, respectively. CONCLUSION: To be aware of the courses of the IMAs at different sternal levels is important during attempts at transthoracic intervention via a parasternal approach.

Adult↗

[Ormiersia carcini gen. n., sp. n., microsporidian of the Mediterranean crab, Carcinus mediterraneus Czerniavsky, 1844: developmental cycle and ultrastructural study].

A microsporidan parasite, Ormieresia carcini gen. n., sp. n., was found in the crab, Carcinus mediterraneus Czerniavsky. Its development and fine structure are the subject are the subject of the present study. The life cycle begins with a schizont surrounded by a unit membrane and containing a diplokaryon. The entire process of sporogony takes place in the host musculature. The sporogonic stages are enclosed in the pansporoblastic membrane. In each pansporoblast, sporogony gives rise to 8 sporoblasts; the octonucleate sporogonial plasmodium is lacking. In the course of schizogonic and sporogonic divisions, each kinetic center consists of 2 plaques, one located within and the other outside the nuclear envelope. The dividing sporonts and sporoblasts sevrets "metabolic" substances (granules, tubules) which are depostied in the pansporoblast. The uninucleate spore is long and cylindrical, measuring 19.1 X 2.4 micronm. A rectilinear manubrium traverses the spore. Its posterior end attenuates abruptly into a polar filament with 4 or 5 coils; its anterior end is attached to the polar cap, which is compressed by a double polar ring. The anterior part of the manubrium is surrounded by a polaroplast consisting of a "spongy" (vesicular) and a lamellar zone.

Animals↗

On the incorporation of moiré shape measurements in finite-element models of the cat eardrum.

The mechanical behavior of the eardrum has previously been shown to depend critically on its shape, but accurate shape measurements have been difficult to make. Phase-shift moiré topography provides a valuable technique for measuring such shapes, and measurement in the presence of large static pressures facilitates the determination of the boundaries of the pars tensa, pars flaccida, and manubrium. New measurements of the shape of the cat eardrum are presented. The presence of hysteresis in the pressure-displacement response is demonstrated. The shapes are incorporated in individualized finite-element models for four different ears, and the variability between and within animals is examined. Fixed-manubrium low-frequency displacements are simulated and compared for the different models.

Animals↗

Papers and articles measurement of central venous pressure in horses.

Central venous pressure measurements were made in 74 horses and ponies free from clinical evidence of cardiopulmonary disease. Using the sternal manubrium as the zero reference point, the mean value obtained was 12 cm H2O (S.D. +/- 6). There was a significant correlation with body weight (r=0.6, p less than 0.001) but there was none with age, sex, breed or type. During halothane anaesthesia, using the same reference point, the mean value was 24.5 cm H2O (S.D. +/- 6) in 28 animals in right lateral recumbency, 29 cm H2O (S.D. +/- 8) in 17 animals in left lateral recumbency and -6 cm H20 (S.D. +/- 4) IN 27 supine animals. The use of the sternal manubrium as zero reference point did not allow comparison of values in standing and recumbent animals and it was considered that serial measurements were of more value than isolated determinations in assessing the circulatory state of an animal.

Acepromazine↗

Vascular pattern of the guinea pig tympanic membrane.

The vascular organization of the guinea pig tympanic membrane was studied by light microscopy in India-ink-, colored-gelatin-, or horseradish peroxidase (HRP)-injected specimens. The ultrastructural localization of the vessels was examined by transmission electron microscopy in HRP-injected material. We found that the tympanic membrane is supplied by two arterial sources, the superior and inferior tympanic arteries, both of which arise from the posterior auricular artery. The superior tympanic artery gives off arterioles which, in the tympanic membrane, run centrifugally from the attachment of the manubrium, while the inferior tympanic artery emits arterioles which run centripetally from the tympanic annulus. Both sets of arterioles divide into capillaries and form a monolayered polygonal meshwork. The capillaries drain into venules which run between arterioles in the membrane and empty into either the superior tympanic vein located at the manubrium or the inferior tympanic vein at the annulus.

Animals↗