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Decay rates of human remains in an arid environment.

The environment of southern Arizona with mild winters and hot, dry summers produces great variability in decay rates of human remains. Summer temperatures, which range well over 38 degrees C (100 degrees F), induce rapid bloating as a result of the accumulation of decompositional gases. However, in certain circumstances, the aridity can lead to extensive mummification, allowing preservation of remains for hundreds of years. A retrospective study of 189 cases, concentrating on remains found on the desert floor or in the surrounding mountains and on remains found within closed structures, outlines the time frame and sequences of the decay process. Remains can retain a fresh appearance for a considerable time in the winter, but the onset of marked decomposition is rapid in the summer months. Bloating of the body usually is present two to seven days following death. Following this, within structures, there is frequently rapid decomposition and skeletonization. With outdoor exposure, remains are more likely to pass through a long period of dehydration of outer tissues, mummification, and reduction of desiccated tissue. Exposure of large portions of the skeleton usually does not occur until four to six months after death. Bleaching and exfoliation of bone--the beginning stages of destruction of the skeletal elements--begins at about nine months' exposure. Insect activity, including that of maggot and beetle varieties, may accelerate decomposition, but this process is greatly affected by location of the body, seasonal weather, and accessibility of the soft tissues. Carnivores and other scavengers also are contributing factors, as are clothing or covering of the body, substrate, elevation, and latitude.

Animals↗

Prenatal and preweaning deaths caused by pseudorabies virus and porcine parvovirus in a swine herd.

Sequential outbreaks of pseudorabies virus and porcine parvovirus infections were documented at a swine farm in southern Minnesota. Data for the prevalence of mummified fetuses born and the preweaning mortality were recorded over a 3-year-period. The farm was a farrow-to-finish facility, with breeding females housed in 4 groups according to their stage of pregnancy. The herd consisted of approximately 130 breeding females in December 1981, and expanded to 220 females during the 12 months of 1982. Excluding the outbreaks, the mean preweaning mortality was 20.43% (SE 1.59) and the number of mummified fetuses per litter was 0.19 (SE 0.01). An outbreak of porcine parvovirus infection caused the preweaning mortality and number of mummified fetuses to increase to 50% and 4.10 per litter, respectively. Two outbreaks of pseudorabies 27 months apart, caused the preweaning mortality to increase to 95% and 82%, and the number of mummified fetuses to increase to 0.96 and 1.25 mummified fetuses per litter, respectively. The increase in mummification was observed 1 month after the increase in preweaning mortality caused by pseudorabies virus infections, whereas the increase in mummification and preweaning mortality was simultaneous with porcine parvovirus infections.

Animals↗

[Treatment of frostbite].

The article shows that treatment of frostbites in a special medium with controlled temperature and humidity makes the time of treatment shorter and prevents complications characteristic of such a trauma. The main purpose of such exposures of the injured areas is to achieve mummification and to make the operation as early as possible. The mummification of the soft tissues began within 5-8 days after trauma. Chemical necrectomy was possible on the 7th-9th days, followed by autodermoplasty on the 2nd day.

Combined Modality Therapy↗

Survey of porcine parvovirus infection in swine fetuses and their dams at a Minnesota abattoir.

Reproductive tracts were recovered from 209 sow and 32 gilt carcasses at slaughter; animals had been pregnant not less than 27 days. Of 241 litters examined, 28 (11.6%) contained one or more porcine parvovirus (PPV)-infected fetuses, as determined by immunofluorescent microscopy. The frequencies in sow and gilt litters were 12.0% and 9.4%, respectively. The PPV antigen was detected in 219 of 334 (65.6%) dead or mummified fetuses and in 12 of 2,172 (0.5%) live fetuses examined. The 18 litters which contained only dead or mummified fetuses were infected with PPV. As the percentage of litter mummification increased, the likelihood of finding PPV increased. The PPV antibody was detected in ovarian follicular fluids of 94.3% of the sows and 78.1% of the gilts. These findings indicate that PPV is highly associated with fetal mummification and that some pregnant gilts and sows are susceptible to infection.

Abattoirs↗

Mummified cutaneous mixed tumor.

A 34-year-old man had an unusual mummified cutaneous mixed tumor that developed shortly after trauma. The apocrine type of tumor cells and pilar differentiation could explain the presence of mummification.

Adult↗

Dwarfs in ancient Egypt.

Ancient Egypt was one of the most advanced and productive civilizations in antiquity, spanning 3000 years before the "Christian" era. Ancient Egyptians built colossal temples and magnificent tombs to honor their gods and religious leaders. Their hieroglyphic language, system of organization, and recording of events give contemporary researchers insights into their daily activities. Based on the record left by their art, the ancient Egyptians documented the presence of dwarfs in almost every facet of life. Due to the hot dry climate and natural and artificial mummification, Egypt is a major source of information on achondroplasia in the old world. The remains of dwarfs are abundant and include complete and partial skeletons. Dwarfs were employed as personal attendants, animal tenders, jewelers, and entertainers. Several high-ranking dwarfs especially from the Old Kingdom (2700-2190 BCE) achieved important status and had lavish burial places close to the pyramids. Their costly tombs in the royal cemeteries and the inscriptions on their statutes indicate their high-ranking position in Egyptian society and their close relation to the king. Some of them were Seneb, Pereniankh, Khnumhotpe, and Djeder. There were at least two dwarf gods, Ptah and Bes. The god Ptah was associated with regeneration and rejuvenation. The god Bes was a protector of sexuality, childbirth, women, and children. He was a favored deity particularly during the Greco-Roman period. His temple was recently excavated in the Baharia oasis in the middle of Egypt. The burial sites and artistic sources provide glimpses of the positions of dwarfs in daily life in ancient Egypt. Dwarfs were accepted in ancient Egypt; their recorded daily activities suggest assimilation into daily life, and their disorder was not shown as a physical handicap. Wisdom writings and moral teachings in ancient Egypt commanded respect for dwarfs and other individuals with disabilities.

Dwarfism↗

Three-dimensional computed tomography of the mummy Wenuhotep.

Computed tomography allows cross-sectional imaging of anthropological as well as clinical subjects. Recently, technical innovations have made three-dimensional reconstruction of these images feasible. We performed two-dimensional and three-dimensional computed tomography of a Late Period Egyptian mummy to reexamine findings seen on previous radiographic studies and to evaluate the usefulness of these techniques in paleopathology. Two-dimensional images provided excellent anatomic detail. There was graphic depiction of the mummification process that corroborated information previously obtained from Egyptological studies. Three-dimensional reconstruction provided images of facial features as if the mummy had been unwrapped. Three-dimensional computed tomography is a useful method of nondestructively evaluating paleopathological remains, and it may yield information not obtainable by any other means.

Female↗

Characterisation of embalming materials of a mummy of the Ptolemaic era. Comparison with balms from mummies of different eras.

Gas chromatography-mass spectrometry has been used to determine the nature of organic materials used in mummification balms. A comparative analysis of samples taken from Egyptian mummies is developed. The results are given in two parts. First, it is shown that the chemical composition of the balm is practically independent of the part of the mummy from which it is taken. This study was done on a Ptolemaic mummy (circa 100 BC from the Guimet Museum in Lyon). Fats, beeswax, and diterpenic resins were the main components: they were found everywhere. Castor oil was also very often detected (in half of the samples). This particular fat is present in the balm inside the thorax but not in the skull. Moreover it is shown that a vegetable tannin was employed. Components indicative of vegetable tannin input (gallic acid and inositols) were found in seven samples out of eighteen, particularly close to the body and on the canopic pack of the heart. Secondly, some conclusions from a comparative study of the composition of balms from mummies of various social levels as well as of different Egyptian periods are reported. It is shown that beeswax was used as from very early times (XVIIIth dynasty). The mixture of beeswax, fats, and diterpenoid resins would appear to be more recent. The balms of three mummies dating from more recent Egyptian periods (XIXth to XXVth dynasty) were analysed. No evidence of a resin, gum-resin, or plant gum could be found. Some mummies would appear to have been embalmed with fats or beeswax. Finally, the entrails canopic pack said to belong to Ramses II undoubtedly shows an embalming process with a triterpenic resin of the mastic type. The adopted analytical methodology enabled us to achieve simultaneous detection of four components of the balm of the Ptolemaic mummy. Analysis of the other five mummies revealed far less complex chemical compositions for the balms. This may be an indication of different embalming processes, although we should bear in mind the question of organic matter preservation through the ages.

Castor Oil↗

Headspace solid phase microextraction for screening for the presence of resins in Egyptian archaeological samples.

The aim of this study was to use headspace solid phase microextraction (SPME) to reveal the presence of resin in archaeological samples, such as mummification balms, from ancient Egypt. Experiments were first performed with fresh resins of known origin. The SPME fibre readily extracted mono- and sesquiterpenes and, to a lesser extent, diterpenes. Using mass spectra and retention indices of constitutive compounds, qualitative analysis of the volatile fraction allowed us to differentiate resins or gum-resins such as myrrh, olibanum, galbanum, labdanum, mastic, and conifer resins. SPME was then successfully applied to archaeological samples from ancient Egypt in which the presence of resins was detected. Volatile components were desorbed and trapped according to the same SPME procedure as was applied to fresh resins, after a sample preparation consisting of a fine grinding.

Journal Article↗

Detection of antibody to encephalomyocarditis virus in mummified or stillborn pigs.

Fetal sera or thoracic fluids of the abnormal fetuses from different swine farms were tested for the presence of antibody to encephalomyocarditis (EMC) virus. Sera of swine fetuses infected experimentally with EMC virus were also tested. High antibody titers ranging 1:128-4096 were detected in both field and experimental samples. Detection of the positive antibody was associated with clinical herd history of increased mummification, stillbirth and neonatal death. The results suggest that EMC virus may be a natural cause of swine reproductive failure and fetal infection can be diagnosed by detecting specific EMC virus antibody in either fetal sera or thoracic fluids.

Animals↗

Maternal serum alpha-fetoprotein levels in a triplet pregnancy with 2 papyraceous fetuses.

Serum Alpha-Fetoprotein (AFP) was found to rise to exceedingly high levels in a case of triplet pregnancy after two fetuses died in the 21st week of gestation. The surviving infant was born in the 36th week accompanied by the two fetus papyracei. By the time the process of mummification of the two dead fetuses appeared to be complete on ultrasound, the maternal AFP level had returned to the normal range for singleton pregnancies. HCG, hPL, and estriol and the coagulation profile remained within the normal range throughout the pregnancy.

Adult↗

[Artificial respiration with technical aids already 5000 years ago?].

Performed at the end of the mummification process, the "mouth-opening" ritual was a ceremony for the spiritual resurrection of the dead Pharaoh. The techniques used are astonishingly similar to modern emergency-room procedures. For example, the mouth-opening device was made of meteoric iron. In the Egypt of that time, the use of iron was very unusual and the higher levels of nickel in meteoric iron contributed to the stability of the instrument. There are other signs of medically sound methods in the mouth-opening ritual. Symbolically, the mummy's teeth were extracted, the eyes opened with a special device, and gold capsules inserted, perhaps in the sense of a breathing tube. There is a possibility that this ritual contains a forgotten synopsis of knowledge gained empirically in connection with the Egyptian cult of royal resurrection.

Egypt, Ancient↗

The Trier mummy Paï-es-tjau-em-aui-nu: radiological and histological findings.

The ancient Egyptian mummy Paï-es-tjau-em-aui-nu, now on exhibition in the Rheinische Landesmuseum in Trier, Germany, was examined by conventional X-ray radiography, computed tomography, and digital fluoroscopy. In addition, some tissues were biopsied for further histologic identification. Along with some representative images, the peculiarities of the Trier mummy concerning the mummification process are presented.

Egypt↗

Fetal varicella syndrome: disruption of neural development and persistent inflammation of non-neural tissues.

Primary varicella zoster virus (VZV) infection during pregnancy is rare. If it occurs between the 8th and 20th week of gestation, fetal varicella syndrome results in 1-2% of the fetuses. We report about a varicella infection that affected a pregnant mother in the 12th week of gestation. At 33 weeks, a premature girl was born with destruction of neurons in spinal cord, spinal ganglia and plexus myentericus, and secondary developmental disturbance including mummification of one arm and segmental intestinal atresia. The brain did not show any abnormalities. However, VZV DNA could be detected by PCR in tissues from the brain and spinal ganglia. Chronic necrotizing inflammation was found in the placenta, fetal membranes, and one ovary. These locations showed nuclear inclusions which by in-situ-hybridization were proven to be VZV derived. This case demonstrates that in the fetal age, 'neurotropism' of VZV signifies severe destruction but not necessarily persistent inflammation of neural tissue. However, due to the inefficient fetal immune system, inflammation can go on for weeks, preferentially in non-neural tissues.

Adult↗

Back to the Egyptians: neurosurgery via the nose. A five-thousand year history and the recent contribution of the endoscope.

The possibility of removing the brain through the nose without disfiguring the face was already known to the ancient Egyptians, who were experts in using this approach in the mummification process. A similar route through the nose and the sphenoid bone has been developed in the last century as a surgical procedure for the treatment of tumors of the pituitary region, which makes the more invasive craniotomy unnecessary in most cases. This has resulted in a significant decrease of the overall mortality and morbidity rates and has led to the great popularity of such minimally invasive surgery both for patients and doctors. The recent advent of the endoscope in this specific field and the development of further possibilities and instrumentation has contributed to a new renaissance of this old treatment modality.

Egypt, Ancient↗

Biochemical analysis of a lithopedion.

Lithopedions are very rare; the medical literature mentions 330 cases. In the present case, a calcified lithopedion was found in the abdominal cavity of a 69-year-old woman who had died of unrelated causes. In general, the different organs of the lithopedion were easily distinguishable macroscopically. Microscopic examination detected reasonably well preserved structure only in skeletal muscle. Dry weight studies showed a definite dehydration (mummification) had taken place. Chemical analysis revealed a shell consisting mostly of inorganic constituents. Quantitative inorganic analysis of tissues was done and results compared to normal tissues. These results showed significant decrease of K and Cl, significant increase in Mg, P, Na and enormous increase in Ca. These data are compatible with the histologically observed calcification of the tissues. Under proper conditions, presumably dormant enzymes were reactivated in brain, liver, and muscle, although the total activity was low. Discernible LDH isoenzymes were found in liver and muscle. Discernible CK isoenzymes were found in brain and muscle.

Aged↗

Possible "immuno-protection" of the bovine parvovirus in the uterus: Preliminary communication.

A pregnant Holstein cow was experimentally inoculated with bovine parvovirus. Approximately five weeks after the last of three injections, the animal aborted a fetus which showed signs of mummification. Virus particles similar to bovine parvovirus were identified by electron microscopy from fetal lung tissue and intestinal contents. Five months after the abortion, the experimental animal died of a corynebacterial metritis and septicemia. Immunofluorescence tests were performed on representative tissues obtained at necropsy. Of all tissues examined only the uterus showed a positive response to the immunofluorescence assay, indicating that the viral antigen persisted in the uterine environment long after its elimination from the systemic circulation.

Journal Article↗

Monoclonal antibodies recognize 2300 years aged alkaline phosphatase.

It was attempted to monitor the immunological response of monoclonal antibodies directed to human alkaline phosphatase in ancient Egyptian bones from the ptolemeic period. The intactness of the respective epitopes of the bone enzyme was successfully demonstrated in an ELISA. Fortunately, the mummified bone was not contaminated by fungi and bacteria due to the fungicidal and bactericidal reactivity of the ancient pretreatment employing resins of pistachio for mummification. The enzyme was enriched using gel chromatography, anion exchange and affinity chromatography to yield 310 +/- 7 mU/mg. The enzymically active fractions of the wheat-germ lectin affinity chromatography were subjected to ELISA. The best binding affinity was detected using the monoclonal antibody BAP A while the reactions of all the other four antibodies BAP B, BAP G, BAP 4A5 and BAP 5D4 were substantially diminished.

Alkaline Phosphatase↗