Search PubMed⌕ Search

SEARCH · Search PubMed

Results for “MASH”

Search indexed PubMed citations on genomics, clinical trials, systematic reviews and public health. Explore titles, authors and supplied subject terms, then open the PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 109 records · Page 6Linked to original sources

Effect of the FAA1 gene disruption of sake yeast on the accumulation of ethyl caproate in sake mash.

Fatty acid activation gene (FAA1) in sake yeast Kyokai no. 701 (K701) was disrupted to investigate the accumulation of ethyl caproate in sake mash. Ethyl caproate, recognized as an important apple-like flavor in sake, is generated by fatty acid synthesis in yeast cells. The disruptant for the FAA1 gene (K701deltafaa1) exhibited a reduced growth rate in a medium containing cerulenin and myristic acid or oleic acid compared with that of the parental strain (K701). In a sake brewing test in which the rice used was polished to 60% of its original size, the fermentation ability of K701deltafaa1 was inferior to that of K701 but the production of ethyl caproate by K701deltafaa1 was 1.6-fold higher than that by K701. These results suggest that the FAA1 gene in sake yeast plays an important role in sake brewing and the accumulation of ethyl caproate.

Journal Article↗

The response of broilers to the feeding of mash or pelleted diets containing maize of varying particle sizes.

1. The effects of maize particle size and steam pelleting on growth and nutrient utilisation were studied with broiler chicks. 2. The presence or absence of 10 microg/kg of 1,25 dihydroxycholecalciferol in diets adequate or deficient in phosphorus was also investigated. Food efficiency was superior with the fine maize diets but calcium retention and phytate phosphorus retention were greatest with the coarse maize diets. Pelleting improved food efficiency and growth in both experiments while phytate phosphorus utilisation was decreased. 3. Addition of 1,25-dihydroxycholecalciferol to the diet increased 16-d body weight, bone ash, plasma dialysable phosphorus and retentions of total phosphorus and phytate phosphorus while decreasing phosphorus deficiency rickets and tibial dyschondroplasia. 4. There were significant interactions between maize particle size and food form. The improvement in calcium retention observed with the coarse maize diets was reduced when the diets were pelleted. When fed as a mash, coarse maize diets resulted in increased plasma dialysable phosphorus levels but when the diet was pelleted this response was eliminated. 5. There was also a significant interaction between particle size and phosphorus concentration in that chicks given diets deficient in phosphorus had improved bone ash when fed coarse maize as compared to fine maize. However, this response was eliminated when the diets were adequate in phosphorus. 6. In one experiment, fine maize diets had higher metabolisable energy values and there was a significant interaction between maize particle size and food form as pelleting improved the metabolisable energy value of coarse maize diets but not fine maize diets. In another experiment only pelleting of the factors studied improved the metabolisable energy value of the diets.

Animal Feed↗

Effects of plastic anti-pecking devices on food intake and behaviour of laying hens fed on pellets or mash.

1. This study investigated effects of plastic anti-pecking devices on food intake and behaviour to assess their applicability in commercial layer flocks. 2. In experiment 1, 72 individually caged ISA Brown pullets were fitted at 16 weeks of age (point of lay) with either 'ring' or 'bumper' beak devices or no device (control), half being fed on pellets and half on mash. The devices were held in place by lugs inserted in the nares. 3. There was evidence of discomfort immediately after fitting the devices, but not thereafter. In the first week, the devices reduced food intake, and were removed from 7 (15%) birds that lost weight consistently. Between 16 and 21 weeks, however, there were no significant effects of beak treatment or food form on either total food intake or egg production with the 65 remaining birds. 4. Beak treatment did not affect feeding efficiency (food intake per minute of feeding). The only behaviour affected was pecking at birds in adjacent cages, which was reduced with the ring device. 5. At 21 weeks, the birds were placed together in groups of 4 to 6 in 12 pens, each group consisting of birds from one of the 6 original treatments, and their behaviour was observed in experiment 2 between 21 and 23 weeks. 6. The only effects of beak treatment on behaviour were that pecking at pen walls was increased with the ring device, while pulling and eating feathers from group mates was seen mainly with control birds fed on pellets. Two birds were cannibalised in a control group on pellets. 7. Devices came off 3 (7%) birds in experiment 1 and were replaced, and rings slipped over lower mandibles of 2 (5%) birds in experiment 2 and were corrected. 8. At 23 weeks, beak lengths were increased with ring and bumper treatments, and pecking damage scores were low with all treatments. 9. It is concluded that these devices are not applicable commercially, but further trials would be justified with new improved designs.

Animal Feed↗

Sugar beet mash silage as a component of a total-mixed-ration for dairy cows--effects on parameters of digestion and animal performance.

Sugar beet mash silage (BMS) was offered in amounts up to 35% of DM to dairy cows as a component of a total-mixed-ration (TMR). Barley and molasses in the control ration were replaced by BMS half in ration BMS 1/2 and in total in ration BMS 1/1 on the basis of the calculated contents of net energy for lactation. Two trials were carried out. In trial I each ration was tested on parameters of rumen fermentation and digestibility of crude nutrients using 4 Holstein cows with rumen fistula. Chewing activity was tested on 2 Holstein cows for each ration. With the BMS rations the ruminal NH3 concentration was lower and the drop in pH was less than in the control ration. The pattern of volatile fatty acids in the rumen fluid from the BMS groups tended towards more propionic and butyric acid. The feeding of BMS showed no negative impact on chewing and rumination. Energy digestibility raised significantly from 59.8% in the control ration to 72.6% in the BMS 1/1 ration. In trial II the same rations were fed in a change-overdesign to a herd of 24 Holstein cows to test feed intake and animal performance. The results showed no significant effects of BMS rations on DM intake and milk production. The results of both trials indicate that even high amounts of cereals can be replaced by BMS without negative effects on rumen fermentation, milk yield and milk composition with slight drop in fat content. For a better handling of BMS, it is of advantage to include it in a TMR.

Ammonia↗

Gastric emptying and glycemic response after ingestion of mashed bean or potato flakes in composite meals.

Two meals of mashed-bean or potato flakes and meat were served in random order to six healthy male subjects to determine effects on gastric emptying and glycemic reactions. The meals had comparable physical appearance and contained similar amounts of digestible carbohydrate, fat, and protein. No difference in gastric emptying, recorded by gamma camera after mixing 51Cr with the meals, was found between the meals. The bean-flakes meal gave significantly lower blood glucose (p less than 0.01) and serum insulin (p less than 0.05) concentrations than did the potato-flakes meal. The overall blood glucose response, calculated as incremental area under the curves for 2 h, also differed between the meals (p less than 0.05) whereas the overall insulin response did not differ significantly. The low glycemic response after bean flakes could not be explained by the gastric emptying rate, which provides additional evidence for the slow digestion of bean starch in the small intestine.

Adult↗

MASH: an interactive program for multiple alignment and consensus sequence construction for biological sequences.

This paper presents a method for the multiple alignment of a sequence set. The MASH algorithm uses a non-redundant database of common motifs and an 'alignment priority' criterion that depends on the length and the occurrence frequency of the patterns in the set of sequences. This user-defined criterion allows the determination of the series of the patterns to be aligned. This program is applied to a fragment of envelope gene env gp120 for 20 isolates of the immunodeficiency virus. The multiplicity of alignments obtained by modifying the criterion parameters reveals different aspects of similarity between the sequences.

Algorithms↗

Lactobacillus satsumensis sp. nov., isolated from mashes of shochu, a traditional Japanese distilled spirit made from fermented rice and other starchy materials.

Gram-positive, rod-shaped, motile lactic acid bacteria (strains NRIC 0603, NRIC 0604T, NRIC 0605 and NRIC 0606) were isolated from shochu mashes using an enrichment culture approach. These strains clustered in the Lactobacillus casei-Pediococcus group and were closely related to Lactobacillus nagelii and Lactobacillus mali on the basis of 16S rRNA gene sequence similarity. Levels of DNA-DNA relatedness revealed genotypic separation of the four isolates from the above two species. The isolates are therefore considered to represent a novel species, for which the name Lactobacillus satsumensis is proposed. The type strain is NRIC 0604T (=JCM 12392T=DSM 16230T).

Alcoholic Beverages↗

Identification of 2,4,6-trichloroanisole (TCA) causing a musty/muddy off-flavor in sake and its production in rice koji and moromi mash.

2,4,6-Trichloroanisole (TCA), which has been identified as the main component responsible for the cork taint in wine, was detected in sake samples having a musty/muddy off-flavor by stir bar sorptive extraction (SBSE). We confirmed that TCA is one of the components causing this off-flavor in sake, as in other alcoholic beverages, from a sensory analysis showing the correlation between TCA concentration and the intensity of the musty/muddy off-flavor. We investigated the route of TCA production in the rice koji preparation process and in the moromi mash process for sake brewing. We found that TCA is produced mainly by the biomethylation of 2,4,6-trichlorophenol (TCP) by rice koji in brewing and that TCP originates from the wooden tools used in preparing rice koji.

Anisoles↗

Production of L-sorbitol from L-fructose by Aureobasidium pullulans LP23 isolated from soy sauce mash.

A strain LP23 that can convert L-fructose to L-sorbitol was isolated from soy sauce mash and identified as Aureobasidium pullulans. The cells grown on L-arabinose were found to have relatively high L-fructose to L-sorbitol conversion potential. Addition of erythritol to the reaction mixture considerably accelerated the conversion rate of L-fructose to L-sorbitol. During the conversion reaction, erythritol was added to the reaction mixture at 8-h intervals to maintain the concentration of erythritol at 1.0%. The final conversion ratios were 82.8%, 95.3%, 92.4%, and 42.6% using washed cells when the concentrations of L-fructose were 1.0%, 2.0%, 5.0% and 10.0%, respectively. The product from L-fructose was identified as L-sorbitol by HPLC analysis, infrared spectroscopy, optical rotation and melting point measurements.

Ascomycota↗

Production of L-talitol from L-psicose by Metschnikowia koreensis LA1 isolated from soy sauce mash.

A strain LA1 that can convert L-psicose to L-talitol was isolated from soy sauce mash and identified as Metschnikowia koreensis. The cells grown on L-arabitol were found to have relatively high conversion potential. Addition of D-sorbitol to the reaction mixture considerably accelerated the conversion rate of L-psicose to L-talitol. During the conversion reaction, D-sorbitol was added to the reaction mixture at 12-h intervals to maintain the concentration of D-sorbitol at 1.0%. The final conversion ratios were 81.4%, 75.2%, 73.0%, 60.4% and 43.5% using washed cells when the concentrations of L-psicose were 0.5%, 1.0%, 2.0%, 3.0% and 5.0%, respectively. The product from L-psicose was identified as L-talitol by HPLC analysis, and infrared spectroscopy, optical rotation and melting point measurements.

Biotechnology↗

Taxonomic homogeneity of a salt-tolerant lactic acid bacteria isolated from shoyu mash.

Forty-seven salt-tolerant lactic acid bacteria, which had been isolated from different places and grown in 15% NaCl, were examined to assess their taxonomic heterogeneity. Among the isolates, 42 were isolated from shoyu mash during the acid fermentation phase, 2 were from miso and 3 were from anchovy pickles. All isolates were identified as Tetragenococcus halophilus on the basis of DNA relatedness values. We further examined 102 phenotypic characteristics of them. The isolates exhibited differences in only 16, supporting the conclusion obtained from the DNA relatedness analysis.

DNA, Bacterial↗

Research note: effects of beak trimming and genetic stock on rate of mash consumption and feeding-related behavior in egg-strain pullets.

Pullets whose beaks were trimmed once (at 9 days) and twice (at 9 days and 9 wk) were able to ingest feed, in the form of mash, more rapidly under competitive feeding conditions and at least as rapidly in the absence of competition as pullets with intact beaks. Tests of feeding rate, when pullets fed in groups, were carried out after a feed deprivation period of 7 h at ages of 10 through 16 wk. Similar tests were done at 18 wk, when pullets fed without competition. Genetic stock and age had significant effects on frequency of agonistic acts at the feeder and displacements from the feeder during 5-min tests under competitive feeding conditions. Age influenced the number of pullets feeding simultaneously and amount of feed eaten per pullet during the same feeding tests. No interactions were detected among beak treatment, stock, and age for feeding-related behaviors or rate of feed consumption during competitive feeding. In noncompetitive feeding tests, genetic stock affected feeding rate but no stock by beak treatment interaction was present.

Aging↗

Orthopedic experience in a MASH unit in postwar Iraq.

The orthopedic experience of a U.S. Army MASH unit deployed in southern Iraq is discussed. Seventy major casualties were surgically treated in a short time span. A high percentage of extremity trauma was observed (69%). Many patients had multiple extremity involvement. The emergency wartime surgical treatment of four specific types of trauma is explored. A new algorithm is presented for the rapid evaluation of penetrating joint injuries. We summarize the current concepts of war surgery as they apply to orthopedic injuries, and add specific observations from this experience.

Hospitals, Military↗

Comparison of pressure and heat resistance of Clostridium botulinum and other endospores in mashed carrots.

Inactivation of bacterial endospores in food requires a combination of pressure and moderate heat. Endospore resistance of seven Clostridium botulinum strains was compared with those of Bacillus spp. (B. cereus, B. subtilis, B. licheniformis, B. smithii, B. amyloliquefaciens) and Thermoanaerobacterium thermosaccharolyticum with respect to pressure (600 to 800 MPa) and temperature (80 to 116 degrees C) treatments in mashed carrots. A large variation was observed in the pressure resistance of C. botulinum spores. Their reduction after treatments with 600 MPa at 80 degrees C for 1 s ranged from more than 5.5 log units to no reduction. Spores of the proteolytic C. botulinum TMW 2.357 exhibited a greater resistance to pressure than spores from all other bacteria examined, with the exception of B. amyloliquefaciens. Heat resistance of spores did not correlate with pressure resistance, either within strains of C. botulinum or when C. botulinum spores were compared with spores of T. thermosaccharolyticum. A quantitative release of dipicolinic acid was observed from C. botulinum spores on combined pressure and temperature treatments only after inactivation of more than 99.999% of the spores. Thus, dipicolinic acid is released by a physicochemical rather than a physiological process. The resistance of spores to combined pressure and temperature treatments correlated with their ability to retain dipicolinic acid. B. amyloliquefaciens, a mesophilic organism that forms highly pressure-resistant spores is proposed as a nonpathogenic target organism for high-pressure process development.

Bacillus↗

Effects of aldehyde dehydrogenase and acetyl-CoA synthetase on acetate formation in sake mash.

To reveal the mechanism of the production of acetate by sake yeast (Saccharomyces cerevisiae), the expression of genes encoding aldehyde dehydrogenase (ALD), acetyl-CoA synthetase (ACS) and acetyl-CoA hydrolase (ACH), which are related to acetate production, was investigated. Northern blot analysis using total RNA of sake yeast isolated from sake mash revealed that all of the tested genes, ACS1, ACS2, ALD2/3, ALD4, ALD6 and ACH1, were transcribed during sake fermentation. Transcription of ALD2/3 was detected only in the early stage of sake fermentation. A static culture of sake yeast in hyperosmotic media including 1 M sorbitol or 20% glucose resulted in high acetate production and increased transcription of ALD2/3. This is the same result as reported in an aerobic condition, and induction of ALD2/3 seemed to be one reason for high acetate production at high glucose concentration during fermentation. Overexpression of ACS2 resulted in low acetate production both during small-scale sake fermentation and in a static liquid culture. On the other hand, over-expression of ACS1 did not change acetate productivity significantly in a static culture. These results indicate that ALD2/3 and ACS2 play important roles for acetate production during sake fermentation.

Journal Article↗

[Effects of solute on growth and intracellular salt concentration of Pediococcus halophilus CCRC 12884 isolated from Inyu mash].

Optimal growth conditions for Pediococcus halophilus CCRC 12884, a halophilic strain of lactic acid bacterium isolated from Inyu (black bean sauce) mash, were MRS broth with 10% NaCl and 35 degrees C of incubation temperature. The NaCl in media could not be replaced by sucrose or glycerol of same concentrations, and under certain osmolarity, growth of P. halophilus CCRC 12884 only in NaCl was better than that in NaCl plus sucrose or glycerol. To determine effects of monovalent and divalent ions on P. halophilus CCRC 12884, it was found that close relations were present among Na+, K+ and PO4(-3). The intracellular concentration of Na+ in P. halophilus CCRC 12884 was not correspondent to its extracellular concentration of NaCl, but intracellular K+ and quaternary ammonium compounds significantly increased as extracellular NaCl increased. Adding 0-3.5 mM betaine to media with different contents of NaCl would not affect the growth of this halophilic bacterium. On feeding 14C-ethanolamine to the bacterium, no intracellular 14C-betaine was synthesized.

Betaine↗

Pad-plate diffusion assay for virginiamycin in mash-type feeds.

An assay procedure has been developed for virginiamycin in mash-type feeds which includes removing fat interferences from the feeds by a rapid petroleum ether wash, a citric acid extraction, an acetone extraction, and the use of the pad-plate diffusion system. The procedure eliminates the need for compensatory curves prepared from feeds of the same or similar composition. Recoveries from 10 laboratory-prepared feeds containing 11.0 micrograms virginiamycin/g averaged 89.3% with a coefficient of variation (CV) of 5.76%. Recoveries from a laboratory-prepared feed containing 13.2 micrograms virginiamycin/g averaged 89.9% (CV 9.92%). The procedure is simple, reasonably accurate, and precise.

Animal Feed↗

MASH genes and the logic of neural crest cell lineage diversification.

Avian embryos have traditionally been the system of choice for studying neural crest development, but the combination of reverse genetics and clonal culture should allow new insights to be gained from mammalian systems as well. We describe one of the first examples where a targeted mutation in a developmental control gene, isolated on the basis of its homology to Drosophila neural determination genes, causes a highly selective phenotype affecting the early development of a subset of neural crest derivatives. Detailed analysis of the cellular phenotype and expression pattern of the gene, called MASH-1, have led to novel insights into the genetic logic that controls neural crest development. Most important, some features of the phenotype appear inconsistent with the predictions of current models of neural crest lineage diversification. These unexpected results have forced a reevaluation of our thinking about some aspects of neural crest development, and illustrate the power of the reverse genetic approach to reveal unanticipated features of complex biological systems as well as to suggest new directions for future study.

Animals↗