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Reaction time to word meaning and ink color of laterally-presented stroop stimuli: effects of handedness and sex.

In two experiments, subjects pressed a key labeled Red or Green in response to a 100 msec duration stimulus presented to the left or right visual field. In Experiment I, subjects responded to the meaning of Stroop words; the stimulus was the word Red or Green printed in red, green, or white ink. In Experiment II, subjects responded to ink color; the stimulus was either the word Red or Green printed in red or green ink or a red or green color patch. In each experiment, there were 20 strongly right-handed subjects and 20 strongly left-handed subjects. Half the subjects in each handedness group were male and half were female. In both experiments, RT was faster when words were presented to the right visual field than to the left visual field, suggesting that both meaning and ink color of Stroop words were processed more quickly in the left hemisphere. Results of both experiments revealed faster reactions when meaning and ink color of the Stroop words were congruent than when they were not. A comparison with baseline trials indicated that the RT difference between responses to congruent and incongruent Stroop words was due to the incongruent cue interfering with information processing rather than to the congruent cue facilitating processing. Hypothesized interactions between stimulus position, congruence, handedness and sex were not significant.

Adolescent↗

[Infiltration of India ink from subarachnoid space to nasal mucosa along olfactory nerves in rabbits].

The mode of the transportation of India ink to the nasal cavity was studied when it was given to the subarachnoid space chiefly through facial nerve sheath and partly by cisterna magna injection. From subarachnoid space India ink infiltrated through perineurium, epineurium and perineural space of the fila olfactoria and of olfactory nerve fibers to lamina propria of the olfactory mucosa and spread also to the respiratory mucosa. It was further taken into lymphatic vessels and accumulated in bilateral cervical lymph nodes. In normal rabbits, India ink didn't penetrate the basement membrane into the epithelial layer at all, but in rabbits with chronic rhinitis showing edema, degeneration and destruction of mucosa, India ink was found to pass easily through the basement membrane into the epithelial layer and further to leak into the nasal cavity in all cases. It was a new finding that in cases with rhinitis large particles like India ink could infiltrate from the subarachnoid space to the nasal cavity passing through the nasal mucosa, and was considered to be a possible cause of idiopathic cerebrospinal fluid rhinorrhea.

Animals↗

HPLC analysis of ballpoint pen inks stored at different light conditions.

A method for comparison of ink entries on documents stored in different light conditions is presented. Various blue inks were exposed to light, both daylight and artificial light from fluorescent tubes. Inks were then extracted from the document and analyzed by HPLC (high performance liquid chromatography). Significant changes in composition were noted on exposure to light. These changes were followed by using ternary diagrams constructed for dyes generally present in blue-colored inks--Crystal Violet, Methyl Violet, and Tetramethyl Para Rosaniline. Also, the amount of the various compounds formed by decomposition of these dyes on exposure to light was measured and employed for comparison of inks. An example of the use of the proposed method in casework is given.

Journal Article↗

Demonstration of digital radiographs by means of ink jet-printed paper copies: pilot study.

Different digital medical images have been printed on paper with a continuous ink jet printer, and the quality has been evaluated. The emphasis has been on digital chest radiographs from a computed radiography system. The ink jet printing technique is described as well as the handling of the image data from image source to printer. Different versions of paper prints and viewing conditions were compared to find the optimum alternative. The evaluation has been performed to maximize the quality of the paper images to make them conform with the corresponding film prints and monitor images as much as possible. The continuous ink jet technique offers high-quality prints on paper at a considerably lower cost per copy compared with the cost of a film print. With a future switch-over from diagnosing of digital images on film to diagnosing them on monitors, hard copies for demonstration purposes will occasionally be needed. This need can be filled by ink jet-printed paper copies.

Copying Processes↗

Phagocytic activity of the reticulohistiocyte system in rabbits after splenectomy and activation with ink.

The survival rate was measured of differently aged rabbit erythrocytes that had been taken from young animals, tagged with 51Cr and injected into young and old animals. The recipient animals had either been splenectomized, or had an ink-activated reticulohistiocyte system, or both. In young as well as in old ink-treated animals the injected cells lived for a much shorter time than in the non-treated animals. In splenectomized animals the red blood cells lived a little longer than those in the control group. In animals which had been both splenectomized and ink-treated the 51Cr-tagged cells again lived for a shorter time than those in the control group, but longer than in the ink-treated animals.

Aging↗

Ballpoint ink flakes as indicators of added entries.

Examiners were asked to determine if an entry made in a diary had been completed at one time, or if the last sentence of the entry had been added later. Standard non-destructive ink differentiation techniques did not show any differences in the ballpoint pen ink used. Dried flakes of ballpoint pen ink were present in the initial stroke of the questioned sentence, but did not appear elsewhere in the entry. Experiments were undertaken to ascertain if these dried flakes could be attributed to a time delay or some other cause. Experimentation showed that ink flakes occurred only after periods of non-use of the ballpoint pens in the sample. Caution is urged in applying this finding to actual case work.

Forensic Medicine↗

Red ink tattoo reactions: successful treatment with the Q-switched 532 nm Nd:YAG laser.

BACKGROUND: In the South-west Thames region there were an unprecedented number of lichenoid tattoo reactions to red ink in patients who had visited a local tattoo parlour. The red ink was found to contain mercuric sulphide, a compound known to cause allergic reactions. Topical Dermovate (clobetasol propionate 0.05%, GlaxoWellcome) ointment alone had little impact. OBJECTIVES: To investigate whether the Q-switched 532 nm Nd:YAG laser could produce permanent flattening of the reaction. METHODS: This was an open nonrandomized clinical trial. Biopsies were taken from the lichenoid areas within the tattoos. Subjects were patch tested to 1% ammoniated mercury in petrolatum prior to treatment with the Q-switched 532 nm Nd:YAG laser. Laser treatments were delivered at 6-weekly intervals by a single operator. Patients also applied topical Dermovate between treatments. Therapy was discontinued when the lesions flattened. Clinical photographs were assessed at baseline and prior to each laser treatment. RESULTS: Seven patients with Fitzpatrick skin types I-III were enrolled in the study (four females, three males, mean age 39 years). All patients completed the trial. Patch testing to mercury was universally negative at 48 and 96 h. Substantial flattening and depigmentation of the red ink within the tattoos was noted after six laser treatments. No adverse effects were recorded. CONCLUSIONS: The Q-switched 532 nm Nd:YAG laser in combination with topical Dermovate ointment is a safe and effective method of treating red ink tattoo reactions.

Administration, Topical↗

Migration of plasticizers from printing inks into foods.

It has been demonstrated that on storage of a tightly wound reel of polypropylene packaging film, specially printed for experimental purposes, transfer can occur of components from the ink on the outer surface of the film on to the inner food contact surface. For dicyclohexyl phthalate this transfer amounted to 6% of the total amount of plasticizer available in the printing ink system. It was subsequently shown for confectionery and snack food products wrapped in commercially printed polypropylene films that plasticizers only present in the printing ink migrated into the foods. The migration of plasticizer increased with storage time of the wrapped product; for dibutyl phthalate, for example, levels increased from 0.2 to 6.7 mg/kg over the period from 0 to 180 days storage of a chocolate-coated confectionery product. A small retail survey (47 samples) of confectionery, snack products and biscuits wrapped in printed polypropylene film showed the presence of one or more plasticizers at levels from 0.02 to 14.1 mg/kg for dibutyl phthalate, from less than 0.01 to 18.6 mg/kg for dicyclohexyl phthalate and from less than 0.01 to 1.8 mg/kg for di(2-ethylhexyl) phthalate. In all cases there was a good correlation between the plasticizers found in the printing ink from the film and those in the food. Wide variations were found, however, in the amounts and types of plasticizers used in printed packaging of the same brand of retail food product purchased from different regions of the country.

Food Contamination↗

Uterine lymphatics: passage of ink and lymphoid cells from the rat's uterine wall and lumen.

The fate of ink and viable lymphoid cells placed in the wall and the lumen of the cycling rat uterus was determined. India ink injected into the myometrium rapidly filled major lymphatic trunks, going to the ipsilateral renal and/or iliac lymph nodes, depending on the site of inoculation along the uterus' length. No other abdominal or thoracic nodes seem to be involved. However, ink injected into the uterine lumen penetrated the endometrial stroma very slowly and only scattered dye-containing phagocytic cells could be seen in draining nodes. Ink-laden macrophages were retained for at least several days in the endometrium. Lymphoid cells similarly injected were traced by several techniques: 1) scintillation counting of tissues for 51Cr-labeled cells; 2) autoradiography for [3H]uridine-labeled cells; 3) fluorescent microscopy for fluorescein-containing cells in frozen tissues; and 4) graft-versus-host reactivity in draining lymph nodes. All techniques gave the same results: cells injected into the myometrium appeared rapidly in draining nodes, inducing a graft-versus-host reaction, whereas cells placed in the uterine lumen were found in only small numbers in the regional nodes, even after several days, and graft-versus-host reactions were minimal or absent. The barrier(s) to cell movement from the uterine lumen remains to be defined, but is of considerable interest since cell traffic in the other direction is a well-documented normal process. The combined effect of restricted cell entrance to the stroma, long retention of macrophages at this site, and an apparent paucity of lymphatic vessels in the endometrium most likely is to slow antigen handling from this site, which may help regulate maternal immune responses during pregnancy.

Animals↗

The value of inking breast cores to reduce specimen mix-up.

Accidental switching of tissue specimens in the histology laboratory can result in significant medical error. We sought to evaluate inking breast core needle specimens as a method to reduce the chance of specimen mix-up. We sequentially inked 1,000 consecutive breast core specimens with 6 different colors. Review of the color of the ink revealed 3 discrepancies: 1 related to blocks being switched, 1 related to incorrect labeling, and 1 was a typographical error. Inking of breast core specimens is a simple, inexpensive, and effective way to help reduce the chance of specimen mix-up.

Biopsy, Needle↗

[Analysis of writing age of ballpoint pen ink by thin-layer chromatography].

A method based on using a thin-layer chromatographic method (TLC) for determining changes in dye of ballpoint pen ink developed with writing age. An ink drawn line of 1 cm in length was cut and placed in Eppendorf tube. Extraction was performed with 20 microL ethanol at room temperature for 15 min, and heated in steambath for 10 min. The ethanol solution(4 microL) containing the extracted dyes was then spotted on TLC plate by syringe after cooling. The ballpoint pen ink spots were eluted in a solvent of isopropyl alcohol. Using TLC scanner, the spots on TLC plate were scanned at 580 nm. The relations between ratio of peak heights and writing age were attained. Thus, the information on relative writing age can be obtained. And, the powerful evidence for identifying the age of ballpoint pen ink is provided by this method in the field of forensic science.

Chromatography, Thin Layer↗

Characterization and aging study of currency ink and currency canine training aids using headspace SPME/GC-MS.

Solid-phase microextraction/gas chromatography-mass spectrometry (SPME/GC-MS) was used to characterize the volatile components associated with U.S. currency, U.S. currency inks, and Canadian currency. Compounds that can be attributed to the ink-curing process include series of straight-chain aldehydes, alkenals, acids, alcohols, and ketones and a series of lactones and 2-alkyl furans. Solvent compounds include naphthenic and paraffinic hydrocarbons with a profile typical of petroleum products, alkyl cyclohexanes, various ethylene glycol alkyl ethers, and traces of chlorinated solvents. Trace levels of 2-phenoxyethanol, a solvent often used in ink formulations, were also detected. Environmental contaminants, those compounds found in circulating currency but not in currency ink, include 2,2'-diethyl-1,1'-biphenyl, methyl benzoate and salicylate, menthol, limonene, dimethyl and diethyl phthalate, and ionol. Not including simple hydrocarbons, over 100 compounds were identified in the headspace of currency-related samples.

Animals↗

Cellular and biophysical mechanisms contributing to regulation of reflex excitability of inking behavior in Aplysia.

Inking behavior in Aplysia offers a simple test system whereby it is possible to examine the cellular and biophysical determinants of a simple behavior and its ability to undergo a short-term behavioral modification. The features of the behavior and its underlying neural circuit, as well as the intrinsic biophysical properties of the effector motor neurons, have been analyzed. Inking behavior shows a selective sensitivity to long-duration stimuli, which appears due to presence of a voltage-dependent fast K+ current and a slow buildup of synaptic input in the ink gland motor neurons. The ability of the behavior to undergo a short-term modification of reflex-excitability appears due to the activation of a prolonged synaptic current in the ink motor neurons.

Animals↗

Ink-bottle effect in mercury intrusion porosimetry of cement-based materials.

Mercury intrusion porosimetry (MIP) is a widely used method for studying porous materials, in particular, cement-based materials. The usual interpretation of such measurements is based on certain assumptions. One of these is that each pore is connected to the sample surface directly or through larger pores. Pores not meeting this assumption are called ink-bottle pores. The effect that sample size has on the MIP characteristics of concrete samples, like the ink-bottle effect and hysteresis, was studied by measuring additional extrusion and intrusion cycles. In order to characterize the extrusion and ink-bottle behavior, the amount of entrapped mercury chi(p) was estimated. Superimposition of extrusion and second intrusion curves is achieved if the contact angle theta is adjusted from theta(i), the intrusion contact angle, to theta(e), the extrusion contact angle. The threshold radius is often assumed to be a dominant pore radius, yet in this study the entrapped mercury content shows no evidence for the presence of a dominant pore radius. Even if characteristic properties of cement-based materials can be estimated with MIP, comparison of results is rendered difficult by the significant effects of sample preparation techniques and sample size and the ink-bottle effect due to randomly present air bubbles.

Journal Article↗

Safety of preoperation endoscopic tattoo with india ink for identification of colonic lesions.

BACKGROUND: Colonic tattooing with india ink is a widely practiced technique regarded as safe, accurate, and reliable. In this series, the largest reported, the safety of this technique is studied. METHODS: A retrospective study of 8,125 consecutive patients who undersent colonoscopy over a 64-month period was conducted. India ink colonic mucosal tattooing was used for either preoperative marking or future endoscopic identification of a lesion. RESULTS: During the study, 195 patients underwent endoscopic injection of india ink. Of these, 50 patients were marked before surgery, and 145 underwent marking with the intent of facilitating future endoscopic localization. Patients were followed by either telephone interviews or physical examination. None of the patients developed fever, persistent abdominal pain, or abdominal tenderness on examination. All surgeons were interviewed. They uniformly reported the tattoo as intensely visible and of great utility in locating the lesions. CONCLUSIONS: Preoperative mucosal tattooing with india ink is recommended as a safe and necessary procedure.

Carbon↗

Application of the micro-FTIR spectroscopy, Raman spectroscopy and XRF method examination of inks.

In routine examination of inks on questioned documents non-destructive analytical methods, such as microscopic and optical techniques are applied first. However, they are often insufficient to identify the inks used for the preparation of the document. In such cases, it is necessary to apply chemical methods that normally cause partial destruction of the examined material. The aim of this work was to evaluate the possibility of discrimination between inks by the use of spectrometric methods, i.e. micro-FTIR spectroscopy, Raman spectroscopy and XRF. About 70 samples of blue and black ballpoint pen and gel inks were examined. It was found that about 90% of the samples of the same type and colour could be distinguished using these methods.

Journal Article↗

Direct-write fabrication of colloidal photonic crystal microarrays by ink-jet printing.

An array of the colloidal photonic crystals was directly fabricated using an ink-jet printing. The colloidal ink droplets containing the monodispersed polystyrene latex particles were selectively deposited on a hydrophobic surface. Solvent evaporation from each ink droplet leads to a formation of microdome-shaped colloidal assembles of close-packed structures. Microspectroscopic analysis has confirmed that the individual assembly serves as a photonic crystal and its optical properties can be correlated with the microstructural features. Unlike other techniques of patterned growth of colloidal photonic crystal, the substrate does not need to be patterned first and no template is needed in the direct writing by the ink-jet printing. Using our strategy, we have rapidly produced the colloidal photonic crystal microarrays composed of different-sized spheres addressably patterned on the same substrate.

Journal Article↗

A fast flexible ink-jet printing method for patterning dissociated neurons in culture.

We present a new technique that uses a custom-built ink-jet printer to fabricate precise micropatterns of cell adhesion materials for neural cell culture. Other work in neural cell patterning has employed photolithography or "soft lithographic" techniques such as micro-stamping, but such approaches are limited by their use of an un-alterable master pattern such as a mask or stamp master and can be resource-intensive. In contrast, ink-jet printing, used in low-cost desktop printers, patterns material by depositing microscopic droplets under robotic control in a programmable and inexpensive manner. We report the use of ink-jet printing to fabricate neuron-adhesive patterns such as islands and other shapes using poly(ethylene) glycol as the cell-repulsive material and a collagen/poly-D-lysine (PDL) mixture as the cell-adhesive material. We show that dissociated rat hippocampal neurons and glia grown at low densities on such patterns retain strong pattern adherence for over 25 days. The patterned neurons are comparable to control, un-patterned cells in electrophysiological properties and in immunocytochemical measurements of synaptic density and inhibitory cell distributions. We suggest that an inexpensive desktop printer may be an accessible tool for making micro-island cultures and other basic patterns. We also suggest that ink-jet printing may be extended to a range of developmental neuroscience studies, given its ability to more easily layer materials, build substrate-bound gradients, construct out-of-plane structure, and deposit sources of diffusible factors.

Animals↗