Search PubMed⌕ Search

SEARCH · Search PubMed

Results for “Individual identification”

Search indexed PubMed citations on genomics, clinical trials, systematic reviews and public health. Explore titles, authors and supplied subject terms, then open the PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 109 records · Page 6Linked to original sources
PubMed2005

Developing a SNP panel for forensic identification of individuals.

Single nucleotide polymorphisms (SNPs) are likely in the near future to have a fundamental role in forensics in both human identification and description. However, considerable research is necessary to establish adequate scientific foundations for these applications. In the case of identification, because allele frequencies can vary greatly among populations, the population genetics of match probabilities is a critical issue. Some SNPs, however, show little allele frequency variation among populations while remaining highly informative. We describe here both an efficient strategy for identifying and characterizing such SNPs, and test that strategy on a broad representation of world populations. Markers with high heterozygosity and little frequency variation among African American, European American, and East Asian populations are selected for additional screening on seven populations that provide a sampling of genetic variation from the world's major geographical regions. Those with little allele frequency variation on the seven populations are then screened on a total of 40 populations ( approximately 2100 individuals) and the most promising retained. The preliminary panel of 19 SNPs, from an initial selection of 195 SNPs, gives an average match probability of <10(-7) in most of 40 populations studied and no greater than 10(-6) in the most isolated, inbred populations. Expansion of this panel to approximately 50 comparable SNPs should give match probabilities of about 10(-15) with a small global range.

DNA Fingerprinting↗
PubMed1997

Image and language in human reasoning: a syllogistic illustration.

Existing accounts of syllogistic reasoning oppose rule-based and model-based methods. Stenning and Oberlander (1995) show that the latter are isomorphic to well-known graphical methods, when these are correctly interpreted. We here extend these results by showing that equivalent sentential implementations exist, thus revealing that all these theories are members of a family of abstract individual identification algorithms variously implemented in diagrams or sentences. This abstract logical analysis suggests a novel individual identification task for observing syllogistic reasoning processes. Comparison of the results of this task with the Standard Task confirms that the tasks are psychologically closely related, throwing light on sources of error, on subjects' sensitivity to metalogical properties, and on term-ordering phenomena. Since it avoids posing the subtask of formulating a quantified conclusion, the new task allows comparison of explanations of problem difficulty in terms of the number of models (e.g., Johnson-Laird & Bara, 1984) with alternatives in terms of the difficulty of choosing a quantifier for the conclusion. Logical concepts of source and conditional premisses provide a comprehensive account of term order data, including figural effects, at a level abstract with regard to imagistic or sentential representations. These results argue that much richer empirical evidence will be required to discriminate phenomenologically distinct reasoning processes than has hitherto been supposed.

Humans↗
PubMed1986

Identification of individual aminoglycoside-inactivating enzymes in a mixture by HPLC determination of reaction products.

A method is described for the detection and identification of two aminoglycoside-acetylating (AAC(2') or AAC(3) or AAC(6')) or -phosphorylating (APH(2'') or APH(3')) enzymes when these are present in the same crude enzyme extract. Crude enzyme extracts were prepared from reference strains of bacteria producing AAC(2'), AAC(3), AAC(6'), APH(2'') or APH(3') enzymes and mixed to give the following paired enzyme combinations: AAC(2') + AAC(3), AAC(2') + AAC(6'), AAC(3) + AAC(6') and APH(2") + APH(3'). These enzyme mixtures were examined by the radioenzymatic profile method for the identification of aminoglycoside-modifying enzymes and their reaction products with butirosin, lividomycin and kanamycin (AAC) or lividomycin and kanamycin (APH) characterized by high performance liquid chromatography (HPLC). Only two of the eight enzymes present were identified by the radioenzymatic method, but all eight were correctly identified by the HPLC method.

Acetylation↗
PubMed1990

Identification of individual renocortical cells that secrete renin.

Successful application of the reverse hemolytic plaque assay was developed to identify individual renocortical cells that secrete renin directly. The plaque assay was validated by a number of established criteria. Using this technique, we demonstrate an increase in renin secretion with beta-adrenergic stimulation and an inhibition of renin secretion with extracellular calcium in groups of renin-secreting cells. Transmission electron microscopy of the cell in the center of a hemolytic plaque demonstrated a modified vascular smooth muscle cell with densely packed secretory granules. Electron microscopy immunocytochemistry demonstrated the presence of renin in the secretory granules, confirming the identity of the cell as a renal juxtaglomerular cell. The technology developed here has allowed the precise identification and study of the individual renin-secreting juxtaglomerular cell.

Animals↗
PubMed1999

[DNA analysis for paternity testing and forensic identification].

Non coding DNA of human genome contains an important part of tandem repetitive DNA which last years has been extensively applied to solve paternity disputes and individual identification cases. A class of this DNA are minisatellites and microsatellites loci. Such loci are highly polimorphics because they have a multiple of different alleles to make them ideals genetic markers for these purposes. This article is a review about these loci, methods of their analysis and practical aspects about their applications to determine biological paternity or individual identification in the forensic genetic field are considered.

DNA↗
PubMed1999

Carboxy terminal variants of Epstein-Barr virus-encoded latent membrane protein 1 during long-term human immunodeficiency virus infection: reliable markers for individual strain identification.

To assess the frequency and molecular polymorphism of malignancy-associated latent membrane protein 1 (LMP1) variants in human immunodeficiency virus type 1 (HIV-1) infection, 94 B-lymphoblastoid cell lines spontaneously derived from peripheral blood mononuclear cells (PBMC) and 30 PBMC samples at seroconversion and later (mean, 55 months) were analyzed by longitudinal comparative sequence analysis in 8 patients progressing to non-Hodgkin's lymphoma (AIDS-NHL), 7 patients to opportunistic infections, and 2 patients with long-term asymptomatic HIV-1 infection. The sequence polymorphism in the C-terminus of LMP1 was characteristic for strains harbored by individual patients, with high fidelity for strain identification. In 14 of the 17 patients, two different but characteristic LMP1 variants were identified. At HIV seroconversion in 8 of 15 patients, a 30-bp deletion (LMP1Delta) was present. Though serial analysis revealed a shift to LMP1Delta in some individuals, statistical analysis of the cohort does not support the hypothesis that accumulation of LMP1Delta variants in PBMC accounts for their observed high incidence in AIDS-NHL.

AIDS-Related Opportunistic Infections↗
PubMed1977

The identification of individuals at high risk for large bowel cancer: an overview.

Attempts to identify individuals with increased susceptibility to colon cancer before clinical manifestations of disease, have recently been made. Early stages of abnormal growth of colonic epithelial cells, and related factors that may contribute to the development of colonic neoplasia have been shown. Based on the identification of early findings, programs to prevent the evolution of malignancy in individuals at increased risk are under consideration at the present time.

Adenocarcinoma↗
PubMed2000

The probability distribution of the number of loci indicating exclusion in a core set of STR markers.

The distribution of the number of loci out of the 13 in the CODIS STR set that would show an exclusion (i.e., a genotype set incompatible either with the prosecution hypothesis or with Mendelian transmission) was estimated in different scenarios. The knowledge of this distribution would provide a framework against which casework evidence can be compared. I used allele frequencies in Iberian and in Italian populations to generate individual genotypes at random and to test in 1 million simulation replicates, how many of the 13 loci would give an exclusion in an individual identification case, a paternity case, and a double parenthood case. All three scenarios were tested under an expected overall exclusion, both for unrelated individuals and for cases in which the suspect or the alleged father was the brother of the real culprit or real father. Paternity and double parenthood cases were also tested in the true scenario, with exclusionary loci due to mutation. In individual identification cases, the average number of exclusionary loci was 11.95 with a minimum of 7. This STR set also showed sufficient power to resolve identification cases in which the evidence sample came from a suspect's sib. False paternity cases yielded an average of 7.65 exclusionary loci and exclusions with only one (0.0108%) or two (0.14%) exclusionary loci were obtained only rarely. The cases of exclusion with one locus could lead to likelihood ratios in favour of paternity, while both true and false paternity cases with two exclusionary loci would often lead to non-conclusive likelihood ratios. The average number of exclusionary loci in a paternity case where the alleged father was the real father's brother was 3.82, with a significant number of cases where no exclusions were obtained.

Child↗
PubMed2005

[Clinical significance and problems of sentinel node identification for individualization of rectal cancer therapy].

In the surgical treatment of advanced rectal cancer, wide lymphadenectomy has been replaced by autonomic nerve preservation because of serious problems such as postoperative urinary and sexual dysfunction. This may offer a considerable clue to as whether metastasis of lateral lymph node can be diagnosed by a new technique in order to provide more benefits to many patients with advanced rectal cancer. Moreover the sentinel lymph node (SLN) concept has lately attracted attention as a new technique. We studied SLN in 40 patients with advanced lower rectal cancer using 99mTc-Sn colloid as a tracer. SLN was successfully identified in 35 (87.5%) of 40 patients, and the mean number of SLNs identified per patient was 5.6 lymph nodes (range 1-14). In this study overall accuracy was 86%, and it was suggested that lateral lymphadenectomy was excluded in 91%, thanks to application of the SLN technique. Although the role this technique plays in the individualization of the therapy for rectal cancer will be large in the near future, many problems remain to be solved in the exact identification of SLN.

Humans↗
PubMed1998

Simulating individual word identification thresholds and errors in the fragmentation task.

This article presents a large-scale study that collected word identification thresholds and errors in the fragmentation task for all four-letter French words. In the first part of this article, we identify some of the variables (e.g., word frequency, neighborhood size, letter confusability) that affect performance in the fragmentation task. In the second part, we analyze individual response performance and identify different response strategies. We demonstrate that the interactive activation model can account for individual response strategies by adapting two of its original parameters: word-letter feedback and letter-word inhibition. In the third part, we demonstrate that the adaptation of the interactive activation model to the fragmentation task makes it possible to successfully simulate a facilitatory frequency effect on identification thresholds, an inhibitory neighborhood size effect on error rates, and an inhibitory letter confusability effect on identification thresholds. When the task-specific processes of the fragmentation task are specified and individual response strategies are considered, the interactive activation model provides a parsimonious architecture for modeling the task-independent processes involved in word perception.

Attention↗
PubMed1991

Analysis of genetic markers in forensic DNA samples using the polymerase chain reaction.

The ability to extract and type DNA from forensic evidentiary samples has revolutionized the field of forensic serology. Previously, genetic marker typing was limited to the analysis of blood group markers and soluble polymorphic protein markers. Because the number of suitable markers expressed in particular fluids and tissues is relatively small, and because mixtures of fluids cannot be separated for conventional genetic marker typing, a suspect frequently cannot be included or excluded as a fluid donor in a case. However, the development of methods to extract DNA from virtually all biological specimens has greatly expanded the potential for individual identification. Of particular importance was the ability to extract mixtures of sperm cells and epithelial cells found in sexual assault cases such that the DNA from the sperm cells could be typed independently of the DNA from the victim's epithelial cells. Restriction fragment length polymorphism (RFLP) analysis was the first DNA-based method applied to problems of individual identification. This method, while powerful in its ability to differentiate individuals, is limited by the quantity and quality of DNA required for an unambiguous result and by the amount of time it takes to obtain a result. Despite these limitations, several laboratories are using RFLP analysis successfully for the detection of polymorphisms in forensic DNA case samples. While the field of forensic serology was being revolutionized by the prospect of DNA analysis, the field of molecular biology was being revolutionized by the invention of the polymerase chain reaction (PCR), which ultimately has had an impact on every area of biological science. The PCR DNA amplification technology is ideally suited for the analysis of forensic DNA samples in that it is sensitive and rapid and not as limited by the quality of DNA as the RFLP method. The focus of this article is the use of the PCR for typing genetic markers, and we will address specifically the special considerations that arise from applying DNA amplification and typing technology to forensic materials.

DNA↗
PubMed1980

A molecular approach to the identification and individualization of human and animal cells in culture: isozyme and allozyme genetic signatures.

The electrophoretic resolution of a group of genetically monomorphic gene-enzyme systems that are developmentally and biologically ubiquitous has been used to provide a species-specific and type-specific biochemical characterization of various cultured cells. The relative mobilities of gene-enzyme systems representing nine distinct gene products from cell cultures of 25 species from Drosophila to man are presented. These isoenzymes effectively discriminate interspecies cell-to-cell contamination and almost invariably serve to identify the contaminating species. The resolution of eight polymorphic gene-enzyme systems in human cell cultures provides a virtually unique allozyme genetic signature as a monitor of intraspecies cellular contamination. The genetic signatures of 47 commonly used human cells are presented. Included in the test were seven putative HeLa (human cervical carcinoma) contaminants each of which expressed a signature identical with that of HeLa. The probability that an unrelated human cell line will have a signature identical to a typed cell is computed for each line from the genotypic frequencies at each locus in a population of cultured human cells. The gene frequencies of this cell population are comparable to the same frequencies in natural human populations. The most common human signature has a frequency (and therefore a probability) of 0.02. The majority of the 17,010 possible signatures are far less probable. A calculation of the theoretical incidence of chance matching of signatures within test groups of two or more individuals is presented. The probability of a chance match between any two randomly selected individuals is 0.004 and among five randomly selected individuals is 0.034. The allozyme genetic signature represents a definitive monitor of cell identity and is presented as a standard of cell and tissue identification for a variety of biological studies.

Animals↗
PubMed1995

Construction of a panel of chromosome-specific oligonucleotide probes (PRINS-primers) useful for the identification of individual human chromosomes in situ.

We present the sequences of a set of oligonucleotides that, when used as primers for PRimed IN Situ (PRINS) labeling, are diagnostic for repetitive sequences in specific human chromosomes. Combined, they enable identification of all human chromosomes except 6, 19, and 20. However, as is also the case with cloned centromeric hybridization probes, chromosomes 14 and 22 are stained together. Along with the sequences of these oligonucleotides we offer a simple, universal procedures for their use. We also present an oligonucleotide that binds to both strands of alpha-satellite DNA, making it possible to specifically amplify alpha-satellite DNA by PCR with this one oligonucleotide alone as primer. The origin of the alpha-satellite DNA in the starting material (whether somatic cell hybrids, flow-sorted chromosomes, or microdissected material) can then be determined on test metaphase spreads by in situ hybridization or PRINS with the PCR product.

Base Sequence↗
PubMed2002

Hypervariable locus of the 3'-flanking region of the neurotensin receptor gene: an effective region for personal identification in forensic practice.

We examined the complex short tandem repeat (STR) locus at the 3'-flanking region of the neurotensin receptor (NTR) gene. The polymorphism of this locus was first reported as a simple tetranucleotide repeat variation by Le et al., but it also offers a surprisingly informative variation, that permits reliable individual identification by two complementary strategies: fluorescent-labelled polymerase chain reaction (PCR)/electrophoresis and direct sequencing of the PCR products. We determined the alleles in 203 Japanese by fluorescent-labelled PCR/electrophoresis. Determination was based on their length with a reliability of +/-1 bp, and the frequency of each allele was very low. Sequencing analysis further grouped these alleles in detail. Sequencing demonstrated that the locus varied by six repetitive units and three insertion/deletion positions of nucleotide fragments. We detected multiple alleles having different structures even in the same allele length. We found structural differences in homozygous alleles having the same base pair size. We also determined that apparently homozygous alleles were heterozygous from sequencing electropherograms showing an overlap of nucleotides or +/-1 bp difference. These results indicate that this locus is structurally hypervariable in addition to having allelic length variations, promising a great advance in individual identification in forensic practice.

Alleles↗
PubMed1997

Perceiving the sex and identity of a talker without natural vocal timbre.

The personal attributes of a talker perceived via acoustic properties of speech are commonly considered to be an extralinguistic message of an utterance. Accordingly, accounts of the perception of talker attributes have emphasized a causal role of aspects of the fundamental frequency and coarse-grain acoustic spectra distinct from the detailed acoustic correlates of phonemes. In testing this view, in four experiments, we estimated the ability of listeners to ascertain the sex or the identity of 5 male and 5 female talkers from sinusoidal replicas of natural utterances, which lack fundamental frequency and natural vocal spectra. Given such radically reduced signals, listeners appeared to identify a talker's sex according to the central spectral tendencies of the sinusoidal constituents. Under acoustic conditions that prevented listeners from determining the sex of a talker, individual identification from sinewave signals was often successful. These results reveal that the perception of a talker's sex and identity are not contingent and that fine-grain aspects of a talker's phonetic production can elicit individual identification under conditions that block the perception of voice quality.

Female↗
PubMed1991

Direct identification of individual vasopressin-containing nerve terminals of the rat neurohypophysis after 'whole-cell' patch-clamp recordings.

The membrane currents in rat neurophypophysial nerve terminals, which contain either vasopressin or oxytocin, have been previously recorded using the 'whole-cell' patch-clamp technique. Interpretation of the electrophysiological data would be significantly strengthened by the ability to correlate them with knowledge of the peptide contents of the terminals being studied. Here, a novel method for detection of the peptide hormone, arginine vasopressin, in those individual isolated terminals is described. The unique aspect of this procedure is that the contents of the terminal are aspirated into the recording electrode after 'whole-cell' patch-clamp recording, and then a highly sensitive dot immunobinding assay allows identification of the peptide contents in the terminals.

Animals↗