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Results for “Immunologic Tests”

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At least 109 records · Page 6Linked to original sources
PubMed1991

[The importance of immunologic tests in endogenous uveitis].

The authors evaluate the results of immunological examination of the peripheral blood of 50 patients with endogenous uveitis hospitalized at the Ophthalmological Clinic in Plzen in 1987-1989. The authors reach the conclusion that with regard to contemporary, available methods we cannot expect a significant contribution of immunological examinations of the peripheral blood for the diagnosis of endogenous uveitis without systemic disease.

Adolescent↗
PubMed1991

Systematization of clinical management for recurrent aborters by combined immunological testing.

The natural outcomes of successive new pregnancies of 66 recurrent aborters were analysed in relation to their immunological profiles. The data demonstrated that the presence of anti-phospholipid antibodies (success rate 6.7%), positive anti-paternal cytotoxicity (8.7%) and a negative mixed lymphocyte reaction (MLR)-blocking effect (33.3%) in the patients' sera were factors associated with a poor prognosis. By the combined evaluation of these testings, a rational scheme of clinical management for recurrent aborters was established.

Abortion, Habitual↗
PubMed1986

Immunologic testing and immunotherapy in recurrent spontaneous abortion.

One hundred sixty-one couples with clinical histories of unexplained recurrent spontaneous abortions were analyzed in part for human leukocyte antigens, antibodies to paternal lymphocytes, and mixed lymphocyte culture reactions. All sera with antipaternal antibodies were investigated on a cell panel and absorbed with various tissues and heparin to help define antigenic specificities, and the couples were categorized as primary or secondary aborters. Primary but not secondary aborters were found to share more human leukocyte antigens with their mates than did 103 control fertile couples. Lymphocytotoxins were rarely identified in primary but were commonly present in secondary aborter sera. Results of mixed lymphocyte culture reactions with primary aborting couples showed an intrinsic cellular inability for the wives to recognize their husbands' cells. Secondary aborting couples' mixed lymphocyte culture reactions also were depressed but as a result of an inhibiting substance in the wives' sera. Thirty-three primary aborting women were treated by immunotherapy with leukocyte infusions as a prophylactic source of trophoblast-lymphocyte cross reactive antigen stimulation to immunologically protect their pregnancies. Eighty-nine percent of primary aborting patients delivered successfully. Six secondary aborting women were treated with heparin therapy; two of these delivered normal infants, two are pregnant, and two have aborted. Clinical implications for immunologic testing and immunotherapy in pregnancy failures are discussed.

Abortion, Habitual↗
PubMed1985

Comparisons of immunological tests for serodiagnosis of Chagas disease in Bolivian patients.

Enzyme linked immunosorbent assay (ELISA) and immunoelectrophoresis (IEP) were evaluated and compared to the classical immunofluorescence (IF) and complement fixation test (CFT) in the immunological diagnosis of Chagas' disease, using 407 sera from Bolivian patients. 72.7 to 79.5% of randomised sera, coming from patients living in endemic areas for Chagas' disease were considered as positive, according to the test limits, previously determined. The techniques could be classified according to their percentage detection as ELISA greater than IF greater than CFT greater than IEP. The quantitative correlations between the tests were excellent (p less than 0.001). 92.8% of the sera were positive or negative for the four tests, 6.1% for three tests and 1.1% for only two tests. The agreement between the tests ranged from 94.6 to 99.2%, co-positivity from 95.5 to 100% and co-negativity from 88.5 to 100%. IF gave the best results, and could be considered as the reference test since it was easy and rapid to perform. However to avoid errors or discrepancies between laboratories, two tests, such as IF and CFT, might be associated. ELISA can be used if higher sensitivity is required. IEP showed 1 to 14 precipitation bands in 96% of the sera from infected patients. The precipitation band 5, previously demonstrated as Trypanosoma cruzi specific, was present in 73% of these sera, indicating the interest to use immunoprecipitation test, if more specificity is required for the immunodiagnosis of Chagas' disease.

Bolivia↗
PubMed1995

Technical report: results of immunological tests on faecal extracts are likely to be extremely misleading.

Clinical investigation of gut immunity is difficult because of the need to study intestinal tissues or secretions directly. Others have reported that immunoglobulins, antibodies and cytokines can be detected in saline extracts of faeces. We have assessed the validity of this approach by measuring immunoglobulins, albumin, alpha 1-antitrypsin and isotype-specific antibodies in matched samples of faeces and whole gut lavage fluid. Results were compared as estimated output per day, and by using haemoglobin as a common reference substance. Samples were obtained from 10 patients with active inflammatory bowel disease and 10 with other benign GI diseases. For immunoglobulins, albumin and antibodies, the amount detected in faeces varied from < 0.01% to 35.5% (based on estimated daily output) and < 0.01% to 18.5% (based on haemoglobin) of the amount known to be produced in the gut from results of assays on whole gut lavage fluid (WGLF); there were significantly higher rates of recovery in faecal specimens from patients with active gut inflammation than from other patients. Detection rates and titres of specific antibody in faeces were even lower than those for immunoreactive IgA. These data indicate that immunological tests on saline extracts of faeces do not represent the true status of the gut humoral immune system, and such studies should be strongly discouraged.

Adult↗
PubMed1997

[Early post myocardial infarction syndrome--a trial of assessment based on clinical course and immunologic tests].

The study analysed clinical and immunological course of 22 patients (aged 56.3 +/- 9.5) with fever (38.5 degrees C) and greatly increased erythrocyte sedimentation rate (ESR) in the first week after myocardial infarction. The control group consisted of 25 patients (aged 50.7 +/- 11.2) without inflammatory and infectious diseases. Clinical courses of the disease, chest x-ray, echocardiography, leucocytosis and serial CK-MB levels were analysed. The immunological investigations involved quantitative estimation of IgG, C3 complement, C4 complement and the identification of T and B lymphocytes on the basis of the rosette tests A, E and EAC. Post myocardial infarction syndrome (PMIS) manifested by fever and greatly increased ESR we observed in 64% of examined patients, but fully manifested PMIS in 14% with tendency to recurrences. We found the differences in immunological examinations compared to the control group. Pericardial effusion occurred in 64% of the patients. The treatment with corticosteroids brought dramatic relief of the symptoms with objective improvement of clinical condition. Patients with high temperature with accompanying greatly raised ESR in the first week after MI may demonstrate abortive form of the post myocardial infarction syndrome.

Aged↗
PubMed1982

[Modern syphilis serology. Immunology, tests and their validity].

In the immunological chapter the appearance of the antibodies in several stages of the lues infection and the methods of demonstration are described. With the lipoid-antigen reactions and the antigen-specific tests modern lues serology can give an answer to the three important questions: Exists a lues infection? Is the demonstrated lues infection active? Was the treatment successful? To diagnose a lues infection one should carry out the Treponema Pallidum Haemagglutination (TPHA) test as screening reaction; the Fluorescent Treponemal Antibody Absorption (FTA-Abs) test should be used for confirmation. The quantitative Cardiolipin Microflocculation test (CMT) decides on the activity of the lues infection and can be used to follow up the effectiveness of treatment. In some cases the Treponema Pallidum Immobilization (TPI) test or the demonstration of the 19S-IgM-antibodies must be used for clarification.

Fluorescent Antibody Technique↗