Role of fetoscopy in prenatal diagnosis.
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Pure fetal blood samples, obtained fetoscopically from 30 patients with unexplained fetal hydrops at 16 to 32 weeks gestation were investigated for cytogenetic, haematological, biochemical and virological properties. In two patients with oligohydramnios, the fetoscope was introduced transabdominally into the fetal peritoneal cavity and sampling was undertaken from the intra-abdominal portion of the umbilical vein; in all the other patients an umbilical cord vessel was sampled. Ten (33%) of the fetuses had chromosomal abnormalities, one an erythroblastic process, possibly erythroleukaemia, one alpha-thalassaemia and one cytomegalovirus infection. Blood-film abnormalities were seen in 23 (88%) of 26 fetuses that had this examination. Biochemical analysis of fetal plasma was undertaken in 18 fetuses and hypoproteinaemia was found in all cases. One fetus was subsequently found to have a paroxysmal tachyarrhythmia that responded to digitilization. Three (10%) of the fetuses survived.
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IMPLICATIONS: We report the administration of two anesthetics to a patient potentially at risk for malignant hyperthermia undergoing fetal surgery and an ex utero intrapartum treatment procedure. Management of anesthesia and intraoperative uterine relaxation with i.v. nitroglycerin to avoid volatile anesthetics are discussed.
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OBJECTIVE: The introduction of the embryofetoscopy--a new method of prenatal diagnosis used in the 1st trimester of the pregnancy. SETTING: Institute for the Care of Mother and Child, Prague. DESIGN: Pilot clinical study. METHODS: The embryofetoscopy was carried out in pregnant women in the 7th or 8th conceptional week who asked for a legal abortion of the pregnancy. We used the embryofetoscope with diameter of 0.8 mm (made by K. Storz, Tuttlingen, Germany). The trocar of the embryofetoscope was inserted into the animal cavity transabdominally always guided by ultrasound. It is possible to perform the examination in local anaesthesia. A further trocar with two canals--one for insertion of the embryofetoscope, the second of the needle with diameter of 0.6 mm--is designated for genetic indicated sampling of embryonal tissues or for incidental application of gene or stem cells therapy in the future. RESULTS: The videocamera documented our embryoscopical findings till this time in 7 cases. On the pictures 1-8 are the face, scull, eye, ear, extremities, genitals and umbilical cord of the embryo in the 7th conceptional week. CONCLUSIONS: The applications of the embryofetoscopy are very wide. It allows us to obtain not only new knowledge about the early development of the normal and affected embryo in vivo, but it simultaneously opens new ways for the therapy of the embryo in the future and for fetal surgery guided by embryoscope in the 2nd and 3rd trimester of the pregnancy.