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Retail consumer acceptance of beef tenderized with calcium chloride.

Beef inside round subprimals were injected with a 200 mM calcium chloride (CaCl2) solution at 5% (wt/wt) to determine its effects on beef steak palatability and quality traits. Alternating subprimals from the right and left sides were selected for injection of CaCl2 or not injected to serve as a control. After 7 d of postmortem storage, 2.5-cm-thick steaks were cut from each subprimal for consumer evaluation (n = 478) in a retail setting. Three supermarkets with customers varying widely in income were selected as test sites for measuring consumer perceptions of the treated and control steaks and package labeling acceptance. Supermarket, income level, education, and sex were evaluated for their impact on acceptance of calcium-injected beef. Steak flavor and tenderness both were important in determining beef eating quality; 50% of consumers said tenderness was the most important and 40% said flavor was the most important. Consumers visually preferred CaCl2-treated steaks 71% of the time over the control steaks based on package labeling. The CaCl2 injection improved tenderness, juiciness, flavor desirability, and overall palatability ratings by the consumers for inside round steaks and did not cause any off-flavor problems compared with the controls. Therefore, injecting beef with a solution of 200 mM CaCl2 at 5% (wt/wt) can improve retail consumer evaluations of beef steak tenderness and reduce tenderness variation without detrimental effects on other palatability or quality traits.

Animals↗

Oral calcium chloride in hypoparathyroidism refractory to massive doses of calcium carbonate and vitamin D.

Surgically induced hypoparathyroidism often responds satisfactorily to intravenous Ca administration, oral CaCO3 and vitamin D2. A 17-year-old girl developed hypoparathyroidism following partial thyroidectomy for thyrotoxicosis. Hypocalcemia was refractory to treatment with massive doses of vitamin D2, up to 150,000 U, 3-6 gm of oral Ca as CaCO3 and 2 micrograms of 1,25-dihydroxycholecalciferol per day. Intravenous Ca gluconate (360 mg of elemental Ca/d, in divided doses) was needed to correct tetany. After 25 days of unsuccessful therapy, oral administration of 30 ml of a 10% solution of CaCl2 (1.09 gm of elemental Ca) was followed by normalization of serum Ca (8.9 mg/dl) within 7 hours. This dose was repeated every 8 hours for 6 days and oral CaCO3 and IV Ca gluconate were discontinued. Serum Ca remained within normal range but hyperchloremic acidosis developed. This was corrected by providing, in addition to vitamin D, 2 g/d of Ca supplementation, 1 gm in the form of 10% CaCl2 solution and 1 gm as CaCO3 in two doses given simultaneously. During 12 months of observation, serum Ca, P and Cl have been consistently within normal limits. This patient was found to have achlorhydria, unresponsive to normalization of thyroid function and serum Ca. These findings indicate that refractoriness to oral CaCO3 and vitamin D may be caused by achlorhydria. Oral administration of CaCl2 solution can promptly correct this defect. Monitoring of serum Cl and CO2 is needed to avoid hyperchloremic acidosis.

Administration, Oral↗

Influence of calcium chloride on systemic acid-base status and calcium metabolism in dairy heifers.

Twenty heifers (12 Holsteins and 8 Jerseys) ranging from 15 to 22 mo of age (SD = 2.2 mo) and weighing 271 to 486 kg (SD = 72 kg) were offered diets containing 0, .5, 1, and 1.5% CaCl2 for 3 wk followed by a 1-wk readjustment period to evaluate the effect of CaCl2 on acid-base status, diuresis, and Ca metabolism. These evaluations were conducted under conditions simulating changes in dietary cation-anion balance that potentially can be utilized as prophylaxis for parturient paresis during transition from dry cow period to lactation. Free proton concentration in blood increased and blood bicarbonate decreased with increasing dietary CaCl2. Plasma protein and blood hematocrit were unaffected by dietary CaCl2. Plasma Ca and urinary hydroxyproline excretion also were unaffected, but urinary Ca excretion rose with increasing dietary CaCl2, possibly reflecting either increased bone mobilization or intestinal absorption of Ca. Elevating dietary Cl increased both plasma Cl and urinary Cl excretion. During the readjustment period, all differences caused by CaCl2 disappeared. Based on the responses of acid-base status and Ca metabolism to the different dietary concentrations of CaCl2, we suggest that feeding 1% CaCl2 to dry cows for 3 wk prepartum could be a suitable method to prevent parturient paresis without causing detrimental acid-base disturbances.

Acid-Base Equilibrium↗

Development of ventricular fibrillation after intravenous calcium chloride administration in a patient with supraventricular tachycardia.

The i.v. administration of calcium before or shortly after treatment of supraventricular tachycardia with verapamil has been suggested to counteract a hypotensive response to verapamil. We discuss the case of a patient who presented to the emergency department with an accelerated wide-complex tachycardia and minimal symptoms. Immediately after i.v. administration, of 1 g calcium chloride as pretreatment for verapamil administration, ventricular fibrillation developed. Emergency physicians should be aware of potential dangers after the administration of i.v. calcium preparations when trying to prevent known hypotensive side effects of i.v. verapamil administration.

Calcium Chloride↗

Calcinosis cutis following calcium chloride electrode paste application for auditory-brainstem evoked potentials recording.

Circumscribed calcification in the dermis (calcinosis cutis) has been reported following electroencephalographic and electromyographic studies at sites of electrode placement following application of an electrode paste containing calcium chloride. Prolonged exposure and skin microabrasions seem to increase the risk of this rare complication of electrode placement, which has not been previously reported following auditory brainstem evoked potential recordings. We report the appearance of localized dermal calcifications at the sites of electrode placement (forehead and retroauricular areas) following auditory brainstem evoked potential recordings in two children, aged 3 and 2 years, respectively. In both cases the electrodes were applied after gentle scrubbing with an ethanol-impregnated gauze and interposition of a calcium chloride bentonite magma paste. The recording took about 1 hour. Erythematous macules and plaques ranging from 4 to 12 mm in diameter, studded with small stony excrescences, appeared at the areas of electrode application 1 and 5 days later, respectively. Histopathologic study of 4 mm punch biopsy specimens showed amorphous dermal deposits of calcium salts. There was superficial ulceration and transepidermal elimination of the calcium deposits, as well as a foreign body tissue reaction in the surrounding dermis. In both patients small stony pellets were extruded during the healing process, which took 11 and 9 weeks, respectively, and left no scars.

Calcinosis↗

Calcification of superficial scalp veins secondary to intravenous infusion of sodium bicarbonate and calcium chloride.

Soft tissue calcification has been reported following the intramuscular injection of calcium salts and the intravenous administration of calcium gluconate. The present case report describes a neonate in whom calcification of the superficial scalp veins developed following administration of calcium chloride and sodium bicarbonate through a scalp vein needle.

Bicarbonates↗

Incorporation of soy milk lipid into protein coagulum by addition of calcium chloride.

Lipid holding in tofu (soybean curd) has such stability that it is not released even when it is cooked. The lipid in soy milk is incorporated into the tofu with coagulation of proteins on processing by the addition of a calcium salt. In this study, the incorporation of lipid into the coagulum was examined from the association with protein. The lipid in soy milk was obtained as the floating fraction by centrifugation. The floating fraction decreased by the addition of calcium chloride prior to forming a protein coagulum. When half of the protein coagulated, all of the floating fraction became inseparable. The protein in soy milk, then, was separated into particulate and soluble fractions by centrifugation. The decrease of the floating fraction with added calcium chloride was parallel to the coagulation of the particulate protein. The association of the floating fraction and the soluble protein occurred after the new particles formed from the soluble proteins. These results indicated that the lipid incorporation took place by the conjugation of the lipid and protein particles.

Beverages↗

[Perforating verruciform collagenoma, an exogenous inclusion skin disease? Apropos of a case induced by calcium chloride].

INTRODUCTION: Perforating verruciform collagenoma, first described in 1963, designates papular and keratotic post-trauma lesions which show an aspect of epidermal perforation microscopically with large fragments of collagen issuing through fistulous chimneys, more or less pycnotic polynuclears and squamating or necrotic epidermal cells. Three publications have presented this diagnosis. CASE REPORT: We observed a patient with multiple lesions with macroscopic and microscopic presentations suggestive of perforating verruciform collagenoma. The lesions appeared after scratches and inoculation with calcium chloride. DISCUSSION: This case is similar to those observed after intradermal inoculation of calcium salts. In the 4 previous cases, perforating verruciform collagenoma was associated with trauma allowing the intradermal penetration of a particular foreign material (glass wool, vegetable debris, drugs or intravenous injections, wound caused by a metallic garbage bin). The exceptional nature of perforating verruciform collagenoma in highly frequent skin wounds would suggest that a very particular post-trauma process is occurring caused by the introduction of an exogenous substance within the derma: calcium chloride in our case, a non-specific material in the four previous cases.

Adult↗

Evaluation of single intratesticular injection of calcium chloride for nonsurgical sterilization of male Black Bengal goats (Capra hircus): a dose-dependent study.

This study describes the induction of chemosterilization in three groups each of six adult male Black Bengal goats at 30 days after a single bilateral intratesticular injection of a calcium chloride (CaCl(2), 2H(2)O) solution at the doses of 10, 20 or 40 mg/kg body weight/testis, always in a 2 ml volume of normal saline. Another one group of animals received only 2 ml of normal saline per testis as a control. The induction of chemosterilization was measured using relative testicular weight as well as histomorphological parameters including seminiferous tubular architecture and germ cell association in seminiferous tubules along with morphology of the interstitial space. Biochemical markers included activities of testicular Delta(5), 3beta-hydroxysteroid dehydrogenase (Delta(5), 3beta-HSD), 17beta-hydroxysteroid dehydrogenase (17beta-HSD), catalase, glutathione peroxidase (GPx), glutathione S-transferase (GST) and superoxide dismutase (SOD) as well as monitoring the level of testicular thiobarbituric acid reactive substances (TBARS), conjugated dienes and reduced glutathione (GSH) content along with plasma concentrations of testosterone, LH and FSH. Histomorphological measures of testes showed total necrosis of testicular tissue at 30 days after an injection of either 20 or 40 mg CaCl(2) along with fibrosis in seminiferous tubules and interstitial spaces. Infiltration of leucocytes was observed with the 40 mg dose. Disintegration of germ cell arrangement in seminiferous tubules and washing out of germ cells from the tubules were noted with the 10mg dose. Relative organ weights, plasma concentrations of testosterone, testicular activities of Delta(5), 3beta-HSD, 17beta-HSD, catalase, GPx, GST, and SOD and testicular contents of GSH all were declined. Increases occurred in testicular TBARS, conjugated dienes and plasma concentrations of LH and FSH with each of the treatments by comparison with the control group. Plasma concentrations of cortisol and fasting blood sugar level as well as packed cell volume (PCV) and total plasma protein were recorded to monitor the changes of chronic stress in the experimental animals. Changes in these parameters were not significant. An intratesticular injection of calcium chloride at specified doses could be a suitable method of sterilization in preference to surgical castration of goats.

Animals↗

[Effect of calcium chloride on interferon production and the biosynthesis of cellular macromolecules].

The influence of calcium chloride in production of interferon and biosynthesis of cell proteins, RNA and DNA in the cultures of chick embryo fibroblast cells and murine cells L-929 in response to induction of the cells by poly(I).poly(C) was studied. It was shown that calcium ions in concentrations of 10 to 30 mM markedly increased formation of interferon in the cell cultures.

Animals↗

Self-setting barrier membrane for guided tissue regeneration method: initial evaluation of alginate membrane made with sodium alginate and calcium chloride aqueous solutions.

Alginate membrane was proposed as a self-setting barrier membrane that can be used for guided tissue regeneration (GTR). The alginate membrane can be prepared and placed at the bone defect during the surgical procedure. The procedure consists of two simple steps. First, the bone defect is filled with sodium alginate (Na-Alg) aqueous solution. Then calcium chloride aqueous solution is dropped on the surface of the Na-Alg aqueous solution. An alginate membrane is formed on the bone defect, keeping the inside of the bone defect filled with unreacted Na-Alg aqueous solution. In this investigation, a preliminary animal study was conducted for an initial evaluation as to whether or not the alginate membrane can be used as a barrier membrane for the GTR method. Bone defects were made in the tibiae of 15-week-old rats. The alginate membrane was made on the surface of existing bone by filling the defect with Na-Alg aqueous solution and then dropping calcium chloride aqueous solution onto the surface of the Na-Alg solution. Four weeks after surgery, the bone defect was found to be reconstructed with new bone when the defect had been covered with alginate membrane whereas the bone defect was filled only with connective tissue when it had been kept open. We concluded, therefore, that this alginate membrane may be a useful barrier membrane when the GTR method is employed.

Alginates↗

Calcium deficiency alters chorda tympani nerve responses to oral calcium chloride.

As a preliminary examination of the influence of calcium deficiency on gustatory sensitivity to calcium, we recorded electrophysiological responses of the chorda tympani nerve of calcium-replete and calcium-deprived rats. Relative to rats fed calcium-replete diet, rats fed low calcium diet had lower electrophysiological response thresholds to CaCl2 (300 vs. 30 microM) and calcium lactate (300 vs. 100 microM) but not NaCl. The calcium-deprived animals had a greater chorda tympani response to 30, 100 and 300 microM CaCl2 and 30 and 100 microM calcium lactate but a smaller chorda tympani response to 30, 100 and 300 mM CaCl2 and to 100 mM MgCl2. A behavioral study using an ascending series of 48-h two-bottle preference tests showed that the threshold for acceptance of CaCl2 was similar to the electrophysiological threshold (310 microM in replete rats, 100 microM in calcium-deprived rats). These findings raise the possibility that changes in calcium status influence the perception of calcium, which, in turn, influences calcium intake.

Animals↗

Influence of calcium chloride fed prepartum on severity of edema and lactational performance of dairy heifers.

Twenty Holstein heifers in a completely randomized design were used to evaluate the effects of prepartum dietary CaCl2 on the development and regression of edema and on subsequent lactational performance. Heifers were assigned to diets containing either 2.17% limestone or 1.5% CaCl2 (DM basis) at 3 wk before expected calving date. Except for the Ca sources, diets were identical. Test diets were only fed prepartum; at calving, all heifers were offered a lactation diet for 3 wk. Severity of edema was evaluated independently by five people daily throughout the experiment; a 10-point rating system was utilized to quantify the severity of edema. Calcium chloride reduced the severity of edema, although this response was most evident during the 1st wk that this salt was fed. The beneficial effects of CaCl2 on edema prepartum disappeared postpartum, when CaCl2 was removed from the diet. Indeed, edema was higher for heifers fed CaCl2 than for those fed limestone at 2 wk postpartum. Calcium chloride reduced DMI prepartum, but, following parturition, a compensatory increase in feed intake occurred. Postpartum daily DMI averaged 1.3 kg higher for heifers fed CaCl2 than for those fed the limestone diet prepartum. Milk yield and composition were somewhat lower for heifers fed CaCl2, although this response was most evident at 1 wk postpartum. Feeding CaCl2 prepartum to prevent milk fever also may reduce the severity of udder edema prepartum and increase feed intake during early lactation.

Animals↗

Effects of calcium chloride injection and hot boning on the tenderness of round muscles.

Two experiments were conducted to determine the effect of CaCl2 injection on round muscles obtained from Bos indicus bulls and late-castrate steers. In Exp. 1, the biceps femoris (BF) muscle from the left side of each of 15 bull carcasses was injected within 30 min postexsanguination with .3 M CaCl2 at 10% by weight while either intact (n = 8) on the carcass or after hot boning (n = 7). The right sides served as controls. In Exp. 2, the semimembranosus (SM) muscles from the carcasses of nine steers (castrated at 16 mo of age) were hot-boned within 30 min postexsanguination and one-half were injected with CaCl2 as described above. Hot boning had no effect (P greater than .05) on shear force values. Calcium chloride injection dramatically reduced shear force requirements at 1, 8, and 14 d postmortem compared with noninjected controls in both experiments. Cooking traits of the SM muscle were not affected (P greater than .05) by CaCl2 injection. However, BF muscles injected with CaCl2 required more (P less than .05) time to cook and had greater (P less than .05) cooking losses than BF controls. Calcium chloride injection of prerigor round muscles reduced aging time needed for normal tenderization to 1 d postmortem. Hot boning was successfully used in conjunction with CaCl2 injection to facilitate the injection process.

Abattoirs↗