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Color images in telepathology: how many colors do we need?

It is generally assumed that for telepathology, accurate depiction of microscopic images requires the use of "true color" (ie, 24 bits, eight bits each for red, green, and blue) in the digitized image used for transmission. If such a 24-bit color image file, which provides a palette of 16.7 million colors, could be reduced in size by decreasing the possible numbers of colors displayed in the image to 8 bits (palette of 256 colors), the image files would require less storage space, could be transmitted more rapidly, and would require less telecommunications bandwidth. However, such color reduction must not result in detectable image degradation, especially if the images are to be used for diagnosis. Therefore, we performed a carefully controlled study to determine whether pathologists could detect differences in the quality of microscopic images that were reduced from 24 to 8 bits of color. Thirty pathologists were each asked to view a set of 30 image pairs displayed on a computer monitor. Each image pair consisted of the original 24-bit color version and an 8-bit color-reduced version, derived using an adaptive color reduction algorithm with diffusion dithering. Observers were asked whether they could detect any difference in quality between the image pairs. Then, regardless of their answer, they were asked to choose the better quality image of the pair. Overall, there was not a statistically significant ability to consciously detect differences between the image pairs (P < .750). However, when forced to choose, there was a significant preference for the 8-bit images as being of "better quality" (P < .005). We conclude that telepathology applications may be able to take advantage of adaptive color reduction algorithms to reduce image file size without sacrificing image quality. Additional studies must be performed to determine the minimal image requirements for accurate diagnosis by telepatholgy.

Color↗

Brightness contrast inhibits color induction: evidence for a new kind of color theory.

A gray region can be made to look colored by a colored surround. This phenomenon, chromatic induction, depends on color differences around the boundary of the region. We performed experiments on chromatic induction with small, initially achromatic, targets on nine different colored surrounds ranging in color from blue to red. Using scaling of saturation as our measure of perceived color strength, we found that chromatic induction is at its maximum when the brightness contrast at the boundary between target and surroundings is minimal. This implies that the neural mechanism in the cerebral cortex that mediates the appearance of brightness at a boundary inhibits the activity of chromatic mechanisms at that same boundary. Observers matched the apparent brightness and luminance of each of the colored surrounds. For surround colors where brightness and luminance matches differ, brightness contrast, not luminance contrast, controls chromatic induction. These new findings, taken together with other evidence, require a new theory of color appearance that includes mutually inhibitory interactions between color and brightness mechanisms that are sensing color and brightness contrast at visual boundaries.

Adult↗

Color category influences heterogeneous visual search for color.

Many previous studies have revealed chromatic characteristics of visual search using relatively simple stimuli. They suggested that color difference between a target and distractors was a crucial factor. However, it may not be applicable to natural environments that contain numerous colors. This study demonstrates the existence of a color-category effect on heterochromatic visual search. Color differences between a target and distractors were constant in the OSA uniform color scales; however, the search times varied widely. This suggests that color differences alone do not explain search performance. To clarify the mediation of a higher-order categorical color process, search times were analyzed using 11 basic colors. When the color category of a target was shared by a larger number of distractors, the search performance declined. However, when the color category of a target was not shared with distractors, the target was easily detected. The results suggest that heterochromatic stimuli could be segregated by categorical color perception.

Adaptation, Ocular↗

Effect of dissolution on color of extrinsic porcelain colorants.

The effect of in vitro dissolution in acidulated phosphate-fluoride gel on the color of metal ceramic samples having surface metal oxide colorants was determined. Three different colorants were applied using two techniques at two different firing temperatures. The color difference was most apparent when the colorant and glaze were combined with only one firing cycle. All colorants increased in value, but changes in color difference were most evident with the blue colorant. It was concluded that dissolution in acidulated phosphate-fluoride gel does affect the color of extrinsic metallic oxide colorants.

Acidulated Phosphate Fluoride↗

Color vision defects in ocular hypertension and glaucoma. Quantification with a computer-driven color television system.

In order to detect early defects of color vision caused by increased intraocular pressure, a computer graphics device and color monitor system were used to measure color contrast sensitivity. The system determines the threshold chrominance of a colored grating in which there is no change in luminance. The study included 13 control subjects aged 10 to 57 years and 19 patients with ocular hypertension or glaucoma aged 20 to 58 years. In the 13 eyes with visual field loss, color contrast sensitivity was profoundly reduced when the grating colors fell on a tritan color confusion line. In the eyes without visual field loss, tritan color contrast sensitivity was reduced to an average level considerably below the extreme limits of the control group. These results were compared with those of other color vision tests and diagnostic criteria for glaucoma. The findings suggest that among the tests used, color contrast sensitivity testing was able to discriminate most effectively between patients who had retinal damage and the normal population.

Color Perception Tests↗

Changes in color and color parameters of dental resin composites after polymerization.

OBJECTIVES: The objectives were to measure the color change of varied shades of dental resin composites after polymerization, and to determine the correlation among the polymerization color change and the changes in color parameters after polymerization. METHODS: Eight light-curing resin composites, a total of 41 shades, were studied. Color of specimens (1 mm in thickness) was measured on a reflection spectrophotometer before and after polymerization over a white background. Changes in color (Delta E*(ab)), and color parameters (Delta L*, Delta C*(ab), Delta a*, and Delta b*: value after polymerization - value before polymerization) were calculated. RESULTS: The range of changes in each shade of resin composites was 1.1-7.9 for color (Delta E*(ab)), -7.5 to 2.3 for Delta L*, -6.8 to 3.1 for Delta C*(ab), -0.9 to 1.2 for Delta a*, and -6.8 to 3.1 for Delta b*. Delta E*(ab), Delta L*, Delta C*(ab), Delta a*, and Delta b* were influenced by the brand and shade of resin composites, and there was a significant interaction between two independent variables (p < 0.05). On the basis of the multiple regression analysis, in which Delta E*(ab) after polymerization was set as a dependent variable and Delta L*, Delta C*(ab), Delta a* and Delta b* as independent variables, multiple correlation coefficient (r) was 0.842 and the included predictors were Delta L* [standardized partial correlation coefficient (beta) = -0.760] and Delta C*(ab) (beta = -0.715). This result indicated that the polymerization changes in color and color parameters were varied by the brand and shade of resin composites, and the polymerization color change was caused by the changes in lightness and chroma with the similar power of influence.

Color↗

Color mixture thresholds measured on a color television--a new method for analysis, classification and diagnosis of neuro-ophthalmic disease.

A color television display can be used to determine color and brightness discrimination thresholds using identical adaptation conditions and experimental technique. The color discrimination threshold is measured by using an equiluminous test spot--i.e. one which differs in color from the surrounding screen but has the same luminance. Because there is no brightness clue, the subject is forced to detect such a spot by using color discrimination. It is shown how color and brightness thresholds may be determined from threshold measurements of different color-mixtures even though it is not known beforehand which stimulus will be equiluminous for the subject. Results are shown for normal subjects, congenital color defectives and for two patients having optic nerve disease who show respectively non-selective and selective loss of color discrimination compared to brightness discrimination. Normal control data are presented, illustrating the effect of eccentricity, optical blur, viewing distance, pupil size and age. It is concluded that the technique is relatively insensitive to moderate variations in these factors and that it is more sensitive in detecting selective color loss than a spectral sensitivity technique which has been described previously.

Adult↗

The relationship between iris color, hair color, and skin sun sensitivity and the 10-year incidence of age-related maculopathy: the Beaver Dam Eye Study.

PURPOSE: To examine the association between iris color, hair color, and skin sun sensitivity and the 10-year incidence of age-related maculopathy (ARM). DESIGN: Population-based cohort study. PARTICIPANTS: A population of 4926 adults (range, 43-86 years of age at baseline) living in Beaver Dam, Wisconsin, was studied at baseline (1988-1990); of these, 3684 and 2764 subjects, respectively, participated in 5-year and 10-year follow-up examinations. METHODS: Data on hair color at age 15 years and skin responsiveness to sun exposure were obtained from a standardized questionnaire administered at the baseline examination. Iris color was determined with penlight illumination during the baseline examination by using photographic standards. Age-related maculopathy status was determined by grading stereoscopic color fundus photos with the Wisconsin Age-Related Maculopathy Grading System. MAIN OUTCOME MEASURES: Incidence and progression of ARM. RESULTS: When controlling for age and gender, people with brown eyes were significantly more likely to develop soft indistinct drusen (risk ratio [RR], 1.53; 95% confidence interval [CI], 1.19-1.97; P < 0.01) than were people with blue eyes. However, people with brown eyes were significantly less likely to develop retinal pigment epithelial depigmentation (RR, 0.58; 95% CI, 0.41-0.82; P < 0.01) than were people with blue eyes. When compared with persons with blond hair, persons with brown hair were at decreased risk of developing pigmentary abnormalities (RR, 0.73; 95% CI, 0.53-1.00; P = 0.05). Iris color, hair color, and skin sun sensitivity were not associated with the development of late ARM. CONCLUSION: Iris color and hair color were found to be associated with the 10-year incidence of pigmentary abnormalities. Iris color seems to be inconsistently related to the 10-year incidence of early ARM lesions and the progression of ARM.

Adult↗

The validity of the University of Waterloo Colored Dot Test for Color Vision Testing in adults and preschool children.

PURPOSE: Most color vision tests require a high level of cognitive ability and as such are problematic for preschool children and multiply challenged individuals. Our goal was to design a color vision test for these groups and evaluate the clinical utility for preschool children. METHODS: The University of Waterloo Colored Dot Test (UWCDot) for Color Vision Testing requires the subject to distinguish a colored disc from seven gray discs. The target disc was a Munsell color along the deutan, protan, or tritan confusion line with gray. The first phase estimated the sensitivity and specificity of the test for adults. Thirty-one adults with normal color vision and 21 adults with congenital red-green defects participated. In the second phase, the utility of the UWCDot test for screening preschool children was determined. Subjects were 281 males and 269 females aged 2.5 to 5 years with normal vision. Their color vision was also assessed with the Standard Pseudoisochromatic Plates, Part 1 (SPP1). RESULTS: The sensitivity and specificity of UWCDot for adults approached the values for the desaturated D-15 when subjective responses were scored. Monitoring fixational eye movements produced sensitivity and specificity values that were similar to the anomaloscope. After adjusting the scoring criterion for the preschool children by using the females as a control, 2.9% of the males were identified as red-green deficient, 1.8% were blue-yellow deficient, and 3.2% had an unclassified deficiency. By definition, 1% of the females failed the test. Counting fixational eye movements was not a useful scoring method in the preschool children. Comparisons with SPP1 indicated that the UWCDot uncovers approximately 35% of the individuals with definite red-green color vision defects. CONCLUSIONS: Our results indicate that the UWCDot is capable of detecting approximately 35% of the preschool children who have a congenital red-green color vision defect. These individuals are likely to have a more severe deficiency.

Adult↗

Color and defective color vision as factors in the conspicuity of signs and signals.

The conspicuity of road traffic signs and signals for a group of observers with the color vision defect of deuteranopia is compared with that for a control group of observers with normal color vision. Conspicuity was measured by the proportion of reports of target objects detected in 300-ms presentations of projected slides of road scenes. There were two instructions, one designed to measure attention conspicuity and the other, search conspicuity. The attention conspicuity of red, orange, and green color-coded traffic control devices was significantly less for deuteranopes than for the observers with normal color vision, but this was not true for yellow and blue color-coded signs. This result is consistent with our understanding of the color perceptions of deuteranopes. The reduction of conspicuity was not so great for the search conspicuity condition. We conclude that redundant color coding does contribute to the conspicuity of signs and signals and that deuteranopes--and probably those with other severe forms of defective color vision--have a significantly reduced ability to notice colored targets, such as road signs and signals, in complex visual environments. The actual and potential application of this work is in the design of signs so they are conspicuous, especially when the user group includes people with defective color vision.

Adolescent↗

Color grouping in space and time: evidence from negative color-based carryover effects in preview search.

Five experiments addressed the role of color grouping in preview search (D. G. Watson & G. W. Humphreys, 1997). Experiment 1 used opposite color ratios of distractors in preview and second search displays, creating equal numbers of distractors in each color group in the final display. There was selective slowing for new targets carrying the majority color of the old items. This effect held when there was no bias in the preview and only the second search set had an uneven color ratio (Experiment 2). In Experiment 3, participants had foreknowledge of the target color, and effects were shown over and above those due to color biases. Experiment 4 demonstrated negative color carryover even when previews changed color. Experiment 5 showed reduced color carryover effects when previews were presented more briefly. Collectively, the results provide evidence for inhibitory carryover effects in preview search based on feature grouping.

Adult↗

A new method for matching tooth colors with color standards.

A new method for quantitative intra-oral tooth color determination is presented. Basically, the tooth color is assessed by visual comparison with opaque color standards, which are logically arranged according to three visual color dimensions. The standards were analyzed spectrophotometrically, and the C.I.E. color coordinates were computed. Illumination and observation were standardized during the matching procedure. Two distinct situations, method 1 and method 2, were investigated. The situation in method 1 is to be considered as large window illumination and small window collection of the reflected light. For method 2, the same small window was used for both illumination and observation. Using both methods, the color of a tooth could be quantified into three separate color dimensions. Using method 1, the consistency among 25 examiners was high in determining the color of ten teeth; using method 2, the inter-examiner agreement was low. For the same tooth, different color standards were selected with method 1 or method 2. The standard selected with method 2 often appeared to be in disagreement within clinical expectations. The differences in results between method 1 and method 2 are explained by the optical properties of the translucent dental enamel (e.g., volume reflection). Method 1 allows for reproducible quantification of clinical tooth discoloration according to C.I.E. color specifications and can possibly be applied in prosthetic dentistry.

Color↗

Short-term memory for colors and color names in the absence of vocalization.

In a previous experiment, Allen found no release from proactive inhibition using the Brown-Peterson procedure in a group who were shifted from recalling colors to recalling the names of colors. The lack of release suggests that colors and color names are encoded in similar ways. It was argued that the similarity of encoding might have been caused by the procedure of requiring the subjects to say out loud the names of the colors at the time of stimulus presentation and recall. In the present experiment, a procedure was devised that eliminated the need for verbalization of the colors. The same pattern of results was obtained, namely, release from proactive inhibition in the group shifted from recalling color names to colors but not in the group shifted in the opposite direction. It was concluded that if subjects encode colors as a verbal label, then this encoding strategy is not caused by the procedure of requiring the subjects to verbalize the colors.

Color Perception↗

Effect of background color, sample thickness, and illuminant on the measurement of broiler meat color.

The effects of sample thickness (0.5, 1.0, and 1.5 cm), background color (white, pink, green, and gray), and illuminant (D-65, A, and F) on color measurement of broiler tissue were determined. Color values from the anterior portions of the two Pectoralis superficialis muscles were not different from each other, allowing one to be used as a control for the other. Light penetrated the thinner posterior portions of the muscle and was reflected by the white background, producing different L*, a*, and b* values when compared to the thicker anterior portion of the muscle. These changes could alter or mask color changes in the tissue. Light penetrated 0.5 cm thick sliced breast, ground breast, and ground thigh samples and was reflected by the background in large enough quantities to cause color differences (P < 0.05) when both sample thickness and background colors were compared. White and pink backgrounds reflected the largest amount of light, followed in decreasing order by green and gray. The light reflected by background decreased as sample thickness increased from 0.5 to 1.0 cm and no differences due to reflection occurred in the 1.5 cm thick samples. Background reflection can prevent measurement of true sample color. The three illuminants produced different (P < 0.05) color values depending on the major wavelength characteristics of each illuminant. Illuminant choice should depend on wavelength characteristics of the illuminant, sample color, and the color values to be measured.

Animals↗

[Color change by thickness and background color on visible light-cured composite resins].

The effects of thickness and background color on the color of five commercial light-cured composite resins were studied by a dental color meter using the CIE 1976 L*a*b* color system. As thickness increased from 1.0 to 4.0 mm, values of L*a*b* decreased for white and dentin color background but showed no obvious difference in color for a black background. the color of composite resins were not in fluenced by background color in a thickness of 4.0 mm. The values of color difference for 1.0 and 2.0 mm thick samples have a larger value than the same 4.0 mm samples. The color difference was recognized even for the same shade name in five representative kinds of composite resins.

Color↗

Color stability of denture base acrylic resins in three food colorants.

STATEMENT OF PROBLEM: Three commonly used artificial dyes in food technology are erythrosine, tartrazine, and sunset yellow. PURPOSE: The color stability of 5 commercially available denture base acrylic resins (QC-20, Meliodent, Trevalon, Trevalon High, and Lucitone) was studied in vitro. METHODS: The specimens were exposed to 3% erythrosine, tartrazine, and sunset yellow solutions at 23 degrees C +/- 1 degrees C. Color changes were determined with a computer-controlled spectrophotometer. Five specimens from each material were processed, and initial color measurements were made after 1, 3, and 6 months of exposure to the staining solutions. Finally, the color stability was quantitatively measured again, and color differences (DeltaE) were calculated. RESULTS: For the observations made in 1, 3, and 6 months intervals, the specimens that exhibited the least color change were in the sunset yellow solution. The greatest color changes observed according to the National Bureau of Standards unit system were Lucitone (2.71) in erythrosine solution, Lucitone (2.54), QC-20 (1.71) in tartrazine solution, and QC-20 (1.66) in sunset yellow solution. The changes in the other acrylic resins in the 3 solutions were slight and at trace level. CONCLUSIONS: All materials tested were acceptable from the standpoint of color stability for long-term exposure to these food colorants.

Acrylic Resins↗

Color changes in the red-green plates of the 50-year-old AO HRR color vision test.

The original AO HRR color vision test has been considered by many as one of the best plate tests. It is still accepted by many governmental agencies for color vision certification. In their 1954 publication, Hardy, Rand, and Rittler stated that specially compounded inks were used for printing to avoid color changes with time. Fifty years later, it is both important and interesting to determine whether the wear and tear cause significant color changes. The chance finding of a never-used second edition offers an opportunity to evaluate the color changes. A GretagMacbeth Spectrolino spectrophotometer was used to measure the chromaticities of the never-used book, and an extensively used book. Four plates (#4, 7, 13, 16), selected randomly from the four red-green sections, were analyzed. The colored dots from each of the eight plates were plotted on a CIE chromaticity diagram. Isocolor lines were drawn to evaluate chromatic alignment. Chromaticities for plates #4 and 7 are significantly different between the two books. With regard to alignment with isocolor lines, the extensively used book is better than the never-used book for plate #4. There is significant misalignment on plate #7 for both books. Chromaticities for plates #13 and 16 are essentially identical between books, all with good alignment with isocolor lines. The overall comparison shows that the chromatic alignment characteristics of the extensively used book are not worse than the never-used book. Since colors in these plates have to be aligned with both the protan and deutan axes, any significant color changes would have disturbed this delicate requirement. The findings of many plates with good alignment, and the lack of differences on plates #13 and 16 between books, suggest that there are no significant color changes over time. Differences between books on plates #4 and 7 were likely the result of the original printing process.

Color↗

Color coordinates for assessment of dietary vitamin E effects on beef color stability.

Color stability was investigated in longissimus lumborum (LL), semimembranosus (SM), and gluteus medius (GM) muscles from Holstein steers fed diets including doses of alpha-tocopheryl acetate that were 0 (EO), 250 (E250), 500 (E500), and 2,000 (E2000) mg.steer-1.d-1 for 42 or 126 d. Longissimus lumborum was aged for 14, 28, and 56 d and GM and SM were aged for 14 d. Effects of vitamin E dose on retention of redness (a*), yellowness (b*), color saturation (chroma), and proportions of redness and yellowness (hue angle) following an aging period of 14 d were E2000 > E500 = E250 > EO (P < .01). Effectiveness of dose duration on the color parameters was 126 d > 42 d (P < .01). Dietary vitamin E supplementation stabilized redness and color saturation, decreased yellowness, and extended color display life of fresh beef. A technique for estimation of color display life based on hue angle measurements of fresh beef is described. Color display life estimates based on hue angle measurements were more consistent with vitamin E supplementation effects on metmyoglobin percentage and hue angle than were estimates obtained from the metmyoglobin threshold method. Color display life across LL, SM, and GM stored until d 14 and then displayed under simulated retail conditions was extended (P < .01) 2.0 (E250) to 5.0 d (E2000). Coefficients of determination for regressions of color display life on muscle alpha-tocopherol concentration were .81, .64, and .63 in LL, SM, and GM muscles aged 14 d, respectively. Supplementation of 500 mg of alpha-tocopheryl acetate per steer daily improved (P < .01) the mean color display life of these three muscles by 2.3 d, or 100%.

Animals↗