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PubMed1975

A heritable hyperlipemic rabbit.

In the course of study on serous cholesterol of rabbit, we found a male mutant with remarkably high cholesterol level (HLR). The characteristics of this mutant could be described as follows: (1) Serous chemical determinations for the mutant's serum revealed extremely high levels of total lipids, and beta-lipoprotein as total cholesterol, being 3011 mg/dl, 10.6 units and 447 mg/dl respectively. High cholesterol level continued these two years during observation, though some fluctuation has been observed. (2) Such hypercholesterolemia was heritable. Determinative gene for hypercholesterolemia seemed to be recessive and not sex-linked, because, while F1 generation did not show so high cholesterolemia, one female rabbit among twelve off-springs born by full-sib mating showed persisting hypercholesterolemia similar to original HLR. (3) HLR may serve as a model of human familial hyperlipemia Type II or III according to the classification by Fredrickson et al.

Animals↗
PubMed2001

Phylogenetic relationships of fig wasps pollinating functionally dioecious Ficus based on mitochondrial DNA sequences and morphology.

The obligate mutualism between pollinating fig wasps in the family Agaonidae (Hymenoptera: Chalcidoidea) and Ficus species (Moraceae) is often regarded as an example of co-evolution but little is known about the history of the interaction, and understanding the origin of functionally dioecious fig pollination has been especially difficult. The phylogenetic relationships of fig wasps pollinating functionally dioecious Ficus were inferred from mitochondrial cytochrome oxidase gene sequences (mtDNA) and morphology. Separate and combined analyses indicated that the pollinators of functionally dioecious figs are not monophyletic. However, pollinator relationships were generally congruent with host phylogeny and support a revised classification of Ficus. Ancestral changes in pollinator ovipositor length also correlated with changes in fig breeding systems. In particular, the relative elongation of the ovipositor was associated with the repeated loss of functionally dioecious pollination. The concerted evolution of interacting morphologies may bias estimates of phylogeny based on female head characters, but homoplasy is not so strong in other morphological traits. The lesser phylogenetic utility of morphology than of mtDNA is not due to rampant convergence in morphology but rather to the greater number of potentially informative characters in DNA sequence data; patterns of nucleotide substitution also limit the utility of mtDNA findings. Nonetheless, inferring the ancestral associations of fig pollinators from the best-supported phylogeny provided strong evidence of host conservatism in this highly specialized mutualism.

Animals↗
PubMed1992

Observer variability in the radiographic measurement and classification of metatarsus adductus.

The classification system of Berg was evaluated using four observers and the radiographs of 42 feet from patients with metatarsus adductus. Interobserver disagreement in diagnosis was 36%. Intraobserver inconsistency averaged 26%. The error range for the lateral and anteroposterior talocalcaneal angle measurement was 13.6 and 15.1 degrees intraobserver and 19.8 and 25.2 degrees interobserver, respectively. There was no correlation between classification and the length of time required for cast correction. The irregularity of hindfoot ossification centers makes measurements inconsistent and seriously reduces the usefulness of classification based on such measurements.

Child↗
PubMed2006

Signalment, clinical signs, and prognostic indicators associated with high-grade second- or third-degree atrioventricular block in dogs: 124 cases (January 1, 1997-December 31, 1997).

OBJECTIVE: To evaluate signalment, clinical signs, and prognosis associated with high-grade second- or third-degree atrioventricular block (AVB) in dogs. DESIGN: Retrospective case series. ANIMALS: 124 dogs. PROCEDURES: Data were gathered from ECGs, veterinarian questionnaires, echocardiograms, and radiographs submitted for review; compared with data from a large control group; and examined for association between variables and duration of survival. A new classification system for AVB was evaluated. RESULTS: Afghan, Catahoula Leopard Dog, Chow Chow, Cocker Spaniel, German Wirehaired Pointer, and Labrador Retriever breeds were predisposed to high-grade second- or third-degree AVB. Heavier, older, and sexually intact female dogs were overrepresented in the study group. Weakness, lethargy, exercise intolerance, and syncope were the most common clinical signs. The presence of clinical signs was not associated with duration of survival. Dogs with high-grade second-degree AVB had a duration of survival similar to that of dogs with third-degree AVB. Dogs with high-grade second- or third-degree AVB were at high risk for sudden death in the first 6 months after diagnosis. High ventricular escape rhythm rate and narrow escape-complex QRS width were negatively associated with duration of survival. Pacemaker implantation had a significant positive association with survival. CONCLUSIONS AND CLINICAL RELEVANCE: Pacemaker implantation should be strongly considered in all dogs with high-grade second- or third-degree AVB regardless of whether clinical signs are evident. If medical treatment is warranted, vagolytic medications may be the best choice. A new classification system for AVB may merit further investigation.

Age Factors↗
PubMed1992

Serum testosterone concentration in response to exogenous GnRH does not predict service rates or pregnancy rates by yearling, performance tested, crossbred bulls.

Ten bulls with a scrotal circumference of less than 30 cm at the end of growth performance testing, and 10 cohorts of the same age, size and breed type with a scrotal circumference greater than 30 cm were used to evaluate if testosterone response following GnRH administration could be used to test for fertility, for semen quality, and for specific pathologic testicular parenchymal changes. Serum testosterone concentrations were determined immediately before and 2 to 3 hours following intramuscular injection of 250 ug GnRH. Bulls were examined for breeding soundness, then fertility was tested in a breeding trial; testicular histology was assessed by determining the percentage of cross-sections of seminiferous tubules with no spermatocytes. The mean (+/-SEM) post-GnRH serum testosterone concentration for all bulls was 11.71 (+/-0.64) ng/ml. In order to examine for an association, the GnRH response was classified as above or below the mean for resultant serum testosterone concentration. The GnRH response classification was not related to the scrotal circumference, percentage of tubules devoid of spermatocytes, or percentage of progressively motile spermatozoa (P>0.10). The percentage of morphologically normal spermatozoa was significantly higher (P<0.05) in the bulls with a higher than mean testosterone secretion in response to GnRH injection. In the breeding trial, the percentage of heifers bred and the percentage of heifers pregnant (60 days post breeding) were not significantly different (P>0.10) between the 2 classifications of GnRH response. The GnRH response test was related to the percentage of morphologically normal spermatozoa but did not predict fertility of yearling bulls in this study.

Journal Article↗
PubMed2006

Evaluation of males spermiogram in domestic pigs.

A microscopic assessment was used to estimate sperm morphological structure in 405 ejaculates from 70 boars according to Blom classification. The classification of spermiogram quality according to a new 6-degree scale was also established. We found, that male spermiograms in domestic pigs were characterized by a large variability both between and within breeds. The estimation of semen quality, only with regard to the mean frequency of occurrence of particular morphological forms of spermatozoa, was not effective for evaluation of individual boar or group of boars. However, the method was effective to evaluate individual ejaculates. The quality classification of the spermiogram enabled to evaluate usefulness of ejaculates graded as good and average for insemination.

Animals↗
PubMed2000

Genetic structure of the Iberian pig breed using microsatellites.

An analysis of 25 microsatellite loci in 210 animals has been used to define the genetic structure of the Iberian pig, traditionally classified into several varieties. In addition, a sample of 20 Duroc pigs was used as an outgroup for topology trees. Inter-variety genetic variation was estimated by unbiased average heterozygosity and the number of alleles observed. Significant deviations from the Hardy-Weinberg equilibrium (HWE) were shown for 19 loci across the whole population. By contrast, equilibrium deviation within varieties was much lower. Genetic variation measures, genetic distance values and a neighbour-joining tree were used to estimate subdivision. In addition, an individual tree was constructed to contrast the assignation of animals into varieties. Despite the low bootstrap values obtained in the varieties neighbour-joining tree, the degree of genetic variation found was sufficient to support the division of the Iberian pig into varieties, although in some cases the traditional classification cannot be accepted. These results have shown the value of this marker panel in the study of intra-breed genetic structures.

Alleles↗
PubMed2004

Epidemiological factors that influence time-to-treatment of trypanosomosis in Orma Boran cattle raised at Galana Ranch, Kenya.

Four thousand nine hundred and seventy-one trypanosomosis-surveillance records from an open population of Orma Boran cattle raised under natural trypanosomosis challenge in Galana Ranch, Kenya between the years 1990 and 2000 were analysed. The objective of the analysis was to identify epidemiological factors that influenced time-to-treatment of trypanosomosis cases. Under the surveillance programme, blood was being examined fortnightly for trypanosomosis using buffy coat technique. Infected animals were treated when their packed cell volumes (PCV) fell to 25% or lower. The number of days between the first diagnosis and treatment of trypanosomosis cases was obtained from the difference between diagnosis and treatment dates. Days-to-treatment clustered around the screening periods; therefore, time-to-treatment was represented by a series of time points 0-8 at 14-day intervals. Factors postulated to affect the outcome (time-to-treatment) were age of an animal at time of diagnosis, sex, number of trypanosome infections, trypanosome species and season of the year. Five animal generations were generated from birth dates and treated as nuisance parameters. Conditional logistic regression and Cox proportional hazards models were fitted to the data, the former to analyse factors that influenced treatment after time 0 (outcome dichotomised as time 0 or >0) and the latter to analyse factors that influenced time-to-treatment for cases that were treated after time 0, excluding all cases treated on time 0. The majority of the cases (89.5%) were treated on the same day of diagnosis. Trypanosome infections were more likely to be treated after time 0 in dry than in wet season. Similarly, the rate of treatment was lower in the dry than the wet season. An increase in number of previous trypanosome infections reduced the odds of an animal being treated after time 0. Animals that had been exposed to many infections before had higher rates of treatment than those that had minimal experiences. We offer possible reasons for these observations and conclude that selection of animals for breeding purposes in programmes geared towards improving trypanotolerance should take into consideration the environmental factors that affect classification of an animal as being resistant or susceptible.

Anemia↗
PubMed2002

Plasmon analysis of Triticum (wheat) and Aegilops. 2. Characterization and classification of 47 plasmons based on their effects on common wheat phenotype.

This article comprises our final remarks on the phenotypic effects of alien plasmons on common wheat. Twenty-one vegetative, reproductive, and seed characters of 551 alloplasmic lines of 12 common wheat genotypes with 46 alloplasmons, and as the control, their euplasmic lines were investigated. Effects of genotype, plasmon, and their interaction had high statistical significance for all the characters investigated, whereas phenotypic variations attributable to the alien plasmons were relatively small. Individual plasmon types are characterized by their primary effects on 21 characters. Genotype x plasmon effects on two representative characters, heading date and plant height, are described in detail. Cluster and principal component analyses of the phenotypic effects of the 47 plasmons yielded 22 groups. The relationships between these phenotype-based groups and those defined by molecular differences in organellar genomes were examined. A significant correlation was found with some explainable discrepancies. For efficient plasmon identification, use of six of the present 12 genotypes is proposed. The key for plasmon classification is provided. Our findings indicate that alien plasmons may be of limited value in future wheat breeding, but that the plasmon diversity that exists in Triticum and Aegilops species is of great significance for understanding the evolution of these genera.

Analysis of Variance↗
PubMed1986

Characterization of lichenoid-psoriasiform dermatosis of springer spaniels.

Three unrelated Springer Spaniels were examined because of chronic dermatitis. Their ages at onset of clinical signs were 4, 5, and 18 months. The initial lesions consisted of erythematous papules and plaques, with corrugated surfaces in the ear canal and in the inguinal area. Later, papules, scale, and adherent keratin mounds developed inside the ear, in the inguinal area, around the mouth, eyes, and anus, and occasionally on the thoracic wall and the limbs. In one case, the nose was involved and there was accompanying depigmentation. The disease waxed and waned for the 10-month, 18-month and 3-year periods of observation, with various treatments proving unsuccessful. Corticosteroids at high doses and antibiotics appeared to mitigate the severity of the disease. Histopathologic interpretation enabled classification of the disease process within both psoriasiform and lichenoid tissue reactions. In view of the apparent breed predisposition and the gross and microscopic appearance of the lesions, it was proposed that the name for this disease be lichenoid-psoriasiform dermatosis of Springer Spaniels. This disease would be clinically classified as seborrhea and could be differentiated within this group by the presence of erythematous papules and plaques along with accurate histologic assessment.

Animals↗
PubMed1993

[Man among the primates. His position after multivariate analysis of his mandible].

Multivariate analysis are applied to 31 ensembles (species of Primates or human breeds), in 31 mandibular linear parameters. A reciprocal averaging show that first factorial plane reproduce the Simpson classification, simplified in 4 groups, with 63.3% of whole inertia. Discriminant analysis calculated with 13 parameters, situates these 4 groups in 3 planes. The D2 Mahalanobis generalised distance is calculated between all groups. The most contributive parameters to generalised distances are inter-molar width and breadth between foramen mentales.

Animals↗
PubMed1994

Follicle development and oocyte maturation during the immediate preovulatory period in Meishan and white hybrid gilts.

A study was carried out to compare preovulatory ovarian events in prolific Meishan gilts (which have high levels of embryo survival) with contemporary white hybrid control gilts. Gilts of similar reproductive ages (second or third oestrous cycle) were observed three times a day for oestrous behaviour and ovaries recovered at a time estimated as within 7 h of ovulation (white hybrid, n = 13; Meishan, n = 16). Preovulatory follicles were recovered (n = 195, white hybrid; n = 252, Meishan), and oocytes were aspirated and fixed for later identification of meiotic stage, and follicular fluid was frozen for subsequent determination of progesterone. The number of presumed ovulatory follicles recovered per animal was similar to the expected ovulation rate (15.0, white hybrid; 15.75, Meishan; P > 0.1); however, follicles from Meishan gilts were smaller (8.1 versus 9.0 mm, P < 0.001) and contained less follicular fluid (139.9 versus 187.6 microliters, P < 0.02) than did white hybrid gilts. Total follicular progesterone (88.7 ng per follicle, white hybrid; 77.4 ng per follicle, Meishan) and concentration of progesterone in follicular fluid (526 ng ml-1, white hybrid; 640 ng ml-1, Meishan) did not differ between the two breeds (P > 0.1). There were no breed differences in the extent of heterogeneity in follicular diameter, volume of follicular fluid, and total follicular progesterone and progesterone concentration (P > 0.1). However, classification of recovered oocytes into seven discrete meiotic stages revealed that more oocytes recovered from Meishan preovulatory follicles were in the more advanced stages of meiosis than were those recovered from white hybrid gilts (P < 0.001).(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMedDate not supplied

Lumbosacral transitional vertebrae in dogs: classification, prevalence, and association with sacroiliac morphology.

The prevalence of lumbosacral transitional vertebrae (LTV) was determined by reviewing the pelvic radiographs of 4000 medium- and large-breed dogs of 144 breeds routinely screened for canine hip dysplasia. An LTV was seen in 138 (3.5%) dogs. The prevalence was higher in German Shepherd dogs and Greater Swiss Mountain dogs than in the other breeds, suggesting a genetic predisposition. There was no gender predisposition. The transverse processes of the LTV were divided into three types based on their morphological characteristics: lumbar type or type 1; intermediate type or type 2; and sacral type or type 3. In a symmetric LTV, both transverse processes are of the same type, while in an asymmetric LTV they are not. The frequency of occurrence of symmetric and asymmetric LTV was similar. In symmetric LTV, intermediate-type transverse processes predominated. Most of the asymmetric LTV had an intermediate-type transverse process combined with a lumbar or sacral type, respectively. Highly asymmetric LTV were often angled relative to the adjacent vertebrae. We hypothesize that an LTV is not the result of transformation of a lumbar into a sacral vertebra or vice versa, but rather is an autonomous intermediate type of vertebra. It occurs when the point of contact of the pelvis with the vertebral column is slightly cranial or caudal to its normal position. The resulting formative stimulus on the vertebral ossification centers, sagittally still separated, causes the various morphologies seen in LTV including the asymmetric variations.

Animals↗
PubMed1998

Isolation and characterization of canine advanced preantral and early antral follicles.

This study was designed to develop preantral follicle isolation and classification protocols for the domestic dog as a model for endangered canids. Ovary donors were grouped by age, size, breed purity, ovary weight and ovary status. Ovaries were randomly assigned to 1 of 3 digestion protocols: A) digestion and follicle isolation on the day of spaying; B) storage at 4 degrees C for 18 to 24 h prior to digestion and follicle isolation; C) digestion on the day of spaying, then incubation at 4 degrees C for 18 h prior to follicle isolation. Minced tissue was placed in a collagenase/DNase solution at 37 degrees C for 1 h. Follicles were classified by oocyte size and opaqueness and by size and appearance of the granulosa cell layers. Preantral follicles contained small, pale oocytes. Preantral follicles containing grown oocytes with dense cytoplasmic lipid were designated as advanced preantral. Only advanced preantral and early antral follicles were examined and classified further. Group 1 follicles had incomplete or absent granulosa layers, Group 2 follicles had several intact granulosa layers, while Group 3 were vesicular (early antral) follicles. Misshapen or pale grown oocytes were classified as degenerated. The percentage of intact germinal vesicles (GV) was recorded for each Group. Digestion Protocol B produced the lowest percentage of degenerated follicles (P < 0.01). Prepubertal donors had fewer (P < 0.01) follicles in each Group and more (P < 0.001) degenerated follicles than older bitches. Larger ovaries yielded the highest total number of follicles (P < 0.05). Ovary status did not affect follicle yield. Oocytes from Group 1 follicles had fewer intact GVs than those from Group 2 or Group 3 (P < 0.0001). These findings provide an opportunity for quantitative studies of the factors regulating folliculogenesis in the domestic dog as a model for endangered canids.

Animals↗
PubMed2002

Factors affecting gestation length in the Thoroughbred mare.

In order to assist in the accurate prediction of the timing of parturition in the mare true gestation length, along with the potential effect of a number of factors, was investigated. Data from 433 Thoroughbred foal pregnancies were used. Sequential ultrasonic scanning allowed the true gestation length (fertilisation-parturition) to be ascertained, as apposed to previous work, which used the mating-parturition interval. An average gestation length of 344.1 +/- 0.49 days was evident. Colt foal pregnancies were significantly (P < 0.001) longer (346.2 +/- 0.72) than fillies (342.4 +/- 0.65). Month of birth had a significant effect on gestation length in all foals (P < 0.001). With foals born in January having the shortest gestation lengths and those born in April the longest. Mare age, year of birth, stallion age, stud farm and the interval between ovulation and mating had no significant effect. It is concluded that (i) the gestation length range (315-388 days), all resulting in viable foals is noteworthy and of clinical importance when considering the classification of dysmaturity in foals, (ii) mares carrying colt foals due to be born in the middle of the breeding season (April) are likely to have the longer gestation lengths.

Age Factors↗
PubMed1994

[Diabetic pregnancy in Senegal (years 1980 to 1989)].

INTRODUCTION AND OBJECTIVES: Diabetic Pregnancy Prognosis in Seventies is it better in Eighties because of Patient Education? METHODOLOGY: Retrospectively, since 1980 to 1989, in Women in status to breed, Diabetic Pregnancy was screened in our Department. W.H.O.'s (1980) Diabetes Mellitus Criterias, PEDERSEN's Criterias, White's Classification were used. Studied Parameters were etiologic factors (age, type, Diabetes oldness and control), former and present Evolution of Pregnancies (term and pregnancy's product). RESULTS: A--In Global Study, 15.07% Diabetic Pregnancies were observed: 22 (37%) Insulin Dependent and 38 (63%) Non Insulin Dependent. The global mean of age was 31 years; Diabetic Mellitus was less than 10 years old in 90% cases whatever the type. Their control was not good (Glycemic Control < 1.4 g/l in less than 50%). Diabetes complications were nephropathy (13.6% and 8%), retinopathy (13.6% and 18.5%), arterial hypertension (0% and 26.32%), cetoacidosis (31.42% and 0%), urinary tract infection (18% and 0%) respectively in Insulin Dependent and Non Insulin Dependent Pregnancies. Near a third (31.6%) of Insulin Dependent and a third (33.41%) for Non Insulin Dependent were of D, F, H White's Class. Multipares were often Non Insulin Dependent and inversely Insulin Dependent frequently paucipare. Former Pregnancies had near 25.5% interrupted term in Insulin Dependents. Present Pregnancies have given 28.5% and 32% of Normal Children: 14.25 and 12% of Abortions and 14.25% and 6% of Spontaneous Premature Parturitions respectively in Insulin Dependents and Non Insulin Dependents. B--In Analytic Study of Foetopathies, bad Prognosis Factors were high Maternal Age, Multiparity, bad Glycemic Control whatever Diabetes type. For Insulin Dependent, Negligency was added and for Non Insulin Dependent, Obesity, Arterial Hypertension and all PBSP. DISCUSSION AND CONCLUSION: A relatively mild Diabetic Pregnancy Prognosis in our areas with limited means has been observed despite of Patients Education.

Adult↗
PubMed2002

Clinical presentations of naturally occurring canine seizures: similarities to human seizures.

The clinical presentations of 119 canine seizures from 41 Standard Poodles and 11 Dalmatians were classified according to a modified version of the International League Against Epilepsy (ILAE) seizure classification system. Standardized use of the ILAE system with dogs not only should facilitate research in veterinary medicine, which has no standard criteria for seizure classification, but also should facilitate comparisons between canine and human seizures. We found that for more than 80% of both breeds, at least some of their seizures had partial onsets. However, because it was common for partial seizures to secondarily generalize, the majority of Poodles (81%) and Dalmatians (91%) experienced at least some generalized seizures. Among partial seizures, complex partial were more frequent than simple partial. For both breeds, two thirds of those with partial onset seizures had exclusively complex partial. Among dogs with primary or secondarily generalized seizures, 80% of both breeds had tonic-clonic seizures.

Journal Article↗