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Isoenzyme variation in schizonts of Plasmodium vivax from Burma.

Fifteen isolates of blood containing Plasmodium vivax were collected from hospital in-patients in Rangoon and the schizonts were harvested for starch gel electrophoresis of the following parasite isoenzymes--glucose phosphate isomerase (GPI) (EC.5.3.1.9), NADP-dependent glutamate dehydrogenase (GDH) (EC.1.4.1.4), lactate dehydrogenase (LDH) (EC.1.1.1.27) and 6-phosphogluconate dehydrogenase (6PGD) (EC.1.1.1.43). Variation was found only in GPI. The other three isoenzymes appeared to be invariant in all the isolates. Forms of GPI were recorded in two isolates of P. vivax which differed from those reported for P. falciparum; these new forms were designated GPI-4 and GPI-5.

Adolescent↗

Trial of Russell's Viper Venoid. I. Immunization of monkeys with venoid. Burma, DMR Working Group on Russell's Viper Venoid.

Refined Russell's Viper Venoid was prepared by the mild and step-wise formalinization of pooled first four fractions obtained by Sephadex G-50 fractionation of Russell's viper venom. This preparation was found to be potent and immunogenic with minimum undesirable side effects. Satisfactory rise of circulating antitoxin levels was seen in monkeys immunized with the venoid and this rise persisted for 24 weeks. The immunized monkeys withstood intramuscular challenge with 2 mg/kg of the crude venom two weeks after a course of venoid injection.

Animals↗

Trial of Russell's Viper Venoid. II. Human immunization with venoid. Burma, DMR Working Group on Clinical Trial of Russell's Viper Venoid.

A previously developed, refined Russell's viper venoid was given to human volunteers to find out if a protective immune response is obtainable and to observe for adverse effects. 12 subjects were immunized by subcutaneous injection of 0.5 ml of venoid followed by two booster injections at four-week intervals and six subjects served as control. Both local and systemic reactions were mild. No abnormality was detected in the laboratory tests performed. Significant rise of antibody titre was detected in all immunized subjects which persisted for 24 weeks. The antibody level achieved in human subjects is equivalent to levels seen in immunized monkeys which withstood doses of venom which would otherwise have been lethal.

Adolescent↗

The efficacy of tourniquets as a first-aid measure for Russell's viper bites in Burma.

The efficacy of the tourniquets commonly used by Russell's viper bite victims in retarding venom movement from the bite was studied in 37 cases by measuring venom antigen levels by enzyme-linked immunosorbent assay in venous samples taken proximal and distal to the tourniquets and also before and after release of tourniquets. In most cases, the tourniquet did not prevent proximal spread of venom. In 8/37 cases, however, venom antigen assays suggested but did not prove that venom absorption was being delayed by the tourniquet.

Antigens↗

Reinfection of people with Ascaris lumbricoides following single, 6-month and 12-month interval mass chemotherapy in Okpo village, rural Burma.

A longitudinal study on reinfection with Ascaris lumbricoides was continued on a random sample of 50% of the infected population following a horizontal cross-sectional study in Okpo village, near Rangoon. The study sample was again randomly divided into two subsamples, the six-month interval worming group and the 12-month interval worming group. Microscopic examination of stool for Ascaris eggs on the 7th day and 30th day, combined with counting eggs and worming with levamisole, were carried out at two successive six-month intervals on the first subsample. Stools were examined and eggs counted on the 7th day and then monthly up to 12 months, followed by worming, in the second subsample. The worms expelled in the first 72 hours after treatment were counted. The findings over a 12-month follow-up period after one treatment included: mean monthly incidence of 20%, higher and more rapid return to previous prevalence and intensity of infection in children or 'wormy' persons than in adults or 'non-wormy' persons. Six-monthly chemotherapy definitely reduced intensity of infection in the children and adults whereas 12-monthly treatment lowered intensity in adults only. Predisposition to acquisition of high or low intensity of infection was also observed. Other findings and the implications of this study for strategies for control of ascariasis are discussed.

Adolescent↗

Age-dependency in predisposition to hookworm infection in the Burma valley area of Zimbabwe.

We examined hookworm re-infection intensities (measured as faecal egg count by the Kato technique) following anthelmintic treatment of farm workers resident on 15 large-scale commercial farms in Zimbabwe. All individuals examined were treated with albendazole (400 mg). Hookworm reinfection intensities of individuals examined, treated and reexamined were compared with the intensities of infection before treatment. Adults had higher than average infection intensities both before and after reinfection. Predisposition to hookworm infection was marked in adults, and absent from younger, less heavily infected hosts. The observations suggest that acquired immunity is unlikely to be an important determinant of hookworm infection and that behaviour may play a major role in determining the observed infection patterns in this community.

Adolescent↗

The environmental history of Chatthin Wildlife Sanctuary, a protected area in Myanmar (Burma).

We reconstructed the history of Chatthin Wildlife Sanctuary (CWS) to understand how social and economic events, and policy changes affected the sanctuary's condition. We surveyed 25 villages surrounding CWS to evaluate past and present ecological conditions, compare the results with historical accounts and identify causal relationships. During the first half of the 20th century, the primary threat was the government's reduction of old growth forest to supply fuel wood for the British-built railway. The railroad opened the area to colonization, but the villagers' impact on timber and wildlife was low. From 1945 to 1988, villagers became the primary force of landscape degradation. The post-war windfall of firearms increased hunting pressure, and populations of large mammal started to decline. With the economic decline of the 1970s and 1980s, the community's demand for game and forest products intensified, and the large mammal fauna was reduced from eleven to four species. From 1988-2003, the forests surrounding the sanctuary were fragmented and degraded. The absence of large predators rendered the park safe for livestock, and the combined effects of grazing and removal of forest products seriously degraded habitat within CWS. Major threats to CWS during the past two decades have resulted from land use decisions in which government-planned economic enterprises caused encroachment by villagers. Stabilization and recovery of this sanctuary will require management compatible with human needs, including expanded buffer zones, better core area protection, community forestry projects, and probably relocation of villages within the park.

Animals↗

Health care in Burma.

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Health Status Indicators↗

Acute and chronic pituitary failure resembling Sheehan's syndrome following bites by Russell's viper in Burma.

Pituitary function was investigated in 9 patients in shock after Russell's viper bites and in 24 individuals who had been severely envenomed 2 weeks to 24 years previously. 3 out of 9 patients had hypoglycaemia and inappropriately low serum cortisol, plasma growth hormone, and plasma prolactin concentrations. 4 who died had pituitary haemorrhage and 1 had adrenal haemorrhage as well. Of the 24 who had apparently recovered from bites, 7 had clinical features of hypopituitarism and no response in plasma growth hormone or prolactin concentrations to symptom-producing insulin-induced hypoglycaemia. 4 of these 7 had a sluggish serum cortisol response to 'Synacthen Depot' and 5 had an abnormal cortisol response to hypoglycaemia. 4 men with symptoms who were tested had low serum testosterone concentrations; serum thyroxine was also low in these men but not in 2 women with menstrual disturbances and impaired insulin responses. Of the 17 individuals without clinical evidence of endocrine disease, 4 had pituitary hormonal abnormalities. Russell's viper envenoming may thus produce a disorder resembling Sheehan's syndrome.

Acute Disease↗