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At least 109 records · Page 6Linked to original sources
PubMed2004

Gene transfer of NAD(P)H oxidase inhibitor to the vascular adventitia attenuates medial smooth muscle hypertrophy.

We previously showed that a systemic inhibitor of gp91(phox) (nox2)-based NAD(P)H oxidase abolishes angiotensin II (Ang II)-induced vascular hypertrophy. In the present study, we tested whether perivascular transfection with Ad-gp91ds-eGFP (an adenoviral bicistronic construct targeting NAD(P)H oxidase in fibroblasts) or controls Ad-CMV-eGFP and Ad-scrmb-eGFP would affect medial hypertrophy in response to Ang II. In C57BL/6J mice, we applied Ad-gp91ds-eGFP or controls to the left carotid adventitia, and 2 days later we implanted minipumps delivering vehicle or Ang II (750 microg/kg per day) for 7 days. None of the viral treatments affected Ang II-induced systolic blood pressure elevation. Immunohistochemical staining showed marker eGFP in adventitial fibroblasts and some macrophages, indicating expression of the gp91ds inhibitor. As expected, Ang II induced medial hypertrophy (medial cross-sectional area, 32.96+/-2.04 versus 20.57+/-1.00x10(3) microm2, Ang II versus control; P<0.001) that was significantly inhibited by Ad-gp91ds-eGFP (26.23+/-0.90x10(3) microm2; P<0.01) but not control viruses. Application of viruses alone did not change medial size under control conditions. Immunohistochemical staining and semiquantitative analysis showed a 70% increase in reactive oxygen species levels measured by the lipid peroxidation byproduct 4-hydroxynonenal (4-HNE) throughout the carotid wall in the Ang II group versus vehicle. After treatment with Ad-gp91ds-eGFP, 4-HNE generation was normalized. Thus NAD(P)H oxidases in adventitial fibroblasts and macrophages appear to modulate Ang II-induced medial hypertrophy.

Adenoviridae↗
PubMedDate not supplied

Elastic elements in the media and adventitia of human intracranial extracerebral arteries.

We find that the media and adventitia of adult human cerebral arteries contain elastic fibers forming a dense, coherent network, similar to that found in muscular arteries of the same size in other organs. The external elastic layer in the adult human is masked for the currently employed staining methods. By treatment with 90% formic acid before fixation, the original staining character of elastic tissue can be restored. The light microscopic and scanning electron microscopic features of this network of elastic fibers are presented.

Adolescent↗
PubMed1986

Technique of experimental aneurysm formation in the rat common carotid artery using the milliwatt carbon dioxide laser and the adventitia patch model.

Aneurysms were induced in the rat common carotid artery by using the milliwatt carbon dioxide laser to weld an adventitia patch over a hole. This technique proved to be effective and reliable as demonstrated by the 100% incidence of aneurysm formation at the patch site in 28 rats killed 1 week or later after the procedure. It is suggested that this technique may be used to produce intracranial aneurysms in experimental animals due to the minimal vessel manipulation required.

Aneurysm↗
PubMed1999

[Cystic degeneration of the popliteal artery adventitia].

The author presents the case-history of a typical clinical picture of a rare vascular affection--cystic degeneration of the adventitia of the popliteal artery in a young man. The diagnostic conclusion was reached by means of duplex sonography and angiography of the extremity, the condition was treated by reconstruction surgery. With regard to its long-term effect the author prefers it to some non-surgical procedures which include in particular transluminal angioplasty or sonographically assisted needle aspiration of the cyst.

Adult↗
PubMed1987

Changes of euchromatin/heterochromatin ratios in cell nuclei of the aortic adventitia in diabetic rats.

The main objective of this study was to determine quantitatively euchromatin/heterochromatin (EU/HET) ratios of cell nuclei in the adventitia of 2 weeks streptozotocin induced diabetic rats combined with acridine orange (AO) ultracytochemistry. Specimens of the aorta above and below the orificium of the a. renalis were treated with AO and then prepared for electron microscopy. Images of 295 nuclei randomly selected from 180 ultrathin sections were analysed by a computer assisted image analyser method. The findings that surface areas of euchromatin portions and those of total nuclei are dependent variables (correlation coefficients 0.93-0.99), however, the EU/HET ratios and total surface areas are independent variables are essential prerequisites for an efficient application of this method. Determinations of EU/HET ratios revealed that populations of AO labeled cell nuclei consistently have higher ratios (2.17-3.38) than nuclei not labeled by AO (1.26-2.12). On the basis of 2583 nuclei counted AO positive cell nuclei were found to increase from 12% in control to 23% in diabetic rats. Changing EU/HET ratios are interpreted in terms of differential RNA synthetic activity enhanced in the early diabetic state. For these cells AO serves as a specific nuclear marker.

Acridine Orange↗
PubMed1994

[Adventitia resection in cystic degeneration of the popliteal artery].

Adventitial cystic disease is a rare form of non-atherosclerotic stenosis of the popliteal artery. It is caused by synovial-like cysts in the subadventitial tissue layer of the arterial wall causing compression of the lumen. The cysts contain mucinous material similar to that found in joint-ganglia. Popliteal artery stenoses causing claudication in young patients is the leading symptom of this disease. The classical therapy is the surgical excision of the diseased artery and interposition grafting with saphenous vein. As an alternative method we present the technique of complete circumferential resection of the diseased adventitia to decompress the lumen of the artery by removing the cysts completely. This technique is called exarterectomy and has been applied in 2 patients. In both cases we could remove the cysts completely without opening the arterial lumen. Intraoperative arteriography documented complete decompression of the arterial lumen. Both patients presented with excellent results 6 months postoperatively without any symptoms or signs of recurrence. Late results of exarterectomy ar not yet available.

Adult↗