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PubMed1996

A novel method for measuring saccade profiles using the scanning laser ophthalmoscope.

Most existing techniques for accurately measuring angular eye position vs time during a saccade (the saccade profile) need either contact to the eye or are restricted in time resolution. In this paper we introduce a new noninvasive method, with high spatial and temporal resolution, for determining saccade profiles using a scanning laser ophthalmoscope (SLO). This method uses the fact that images of a moving object taken with the SLO are not blurred (as are images from video cameras) but show a tilt with respect to images of the same stationary object. A mathematical framework is given that allows determination of a saccade profile from a restricted number of consecutive SLO video fields of the fundus during a saccade. The angular resolution obtained by this method is below 0.1 deg, and the maximum time resolution near 1 msec. Measured saccade profiles could be well approximated by a gamma function, the first derivative of which yields the saccade velocity profile. Measurements of peak saccade velocity as a function of saccade amplitudes (main sequence) using our method show good agreement with results obtained by other authors.

Humans↗

PubMed1990

Quantitation of collagen fibril cross-section profiles in aging human veins.

The cross-section profiles and the diameter distribution of collagen fibrils were examined quantitatively in normal human internal jugular veins at different ages (first, fifth, and eighth decades). All fibrils showed a regular cross-striation pattern of native-type collagen fibrils irrespective of their cross-section profiles. Irregularly outlined ("dysplastic") fibrillar profiles were observed among the normally occurring circular cross-section profiles. The frequency of such unusual fibrils significantly increased with age. This increase was more pronounced in the tunica media as compared with the tunica adventitia. In the tunica media diameters of collagen fibrils also generally increased with age. In the tunica adventitia, on the other hand, fibrillar diameters were not significantly altered at different ages. The results of this study indicate that the frequency of both the irregularly outlined fibrillar cross-section profiles and increased fibrillar diameters depends on age in normal vascular walls. Therefore, it is concluded that the occurrence of "dysplastic" fibrils is a physiologic age-related phenomenon rather than a morphologic sign of pathologic alteration of collagen. The higher frequency of irregularly outlined collagen fibrils in the tunica media may indicate a higher and/or altered synthetic behavior of smooth muscle cells when compared with fibroblasts of the tunica adventitia.

Adult↗

PubMed1988

The chromosome profiles of Trypanosoma congolense isolates from Kilifi, Kenya and their relationship to serodeme identity.

Chromosomal DNA from 117 Trypanosoma congolense clones from 54 stocks, isolated from cattle introduced onto a ranch in Kilifi in the coastal area of Kenya, was fractionated by the orthogonal field alternation gel electrophoresis technique. The technique resolved chromosomes in the size range of 100 kb-1 Mb. The chromosome profile for cloned trypanosome populations was relatively stable with regard to number and size of the chromosome bands following transmission in mice, cattle, goats or tsetse flies. Only in one clone was a shift observed in the position of one medium-sized chromosome band following cyclical development in tsetse. On the basis of their chromosome profiles, the 117 clones could be divided into 18 distinct groups. Representative clones, randomly selected from 7 of the 18 chromosome profile groups were inoculated into steers and goats in order to raise variable antigen type (VAT) repertoire-specific infection sera. Cross-neutralization assays demonstrated that recovery sera from animals infected with a clone neutralized all the clones with an identical chromosome profile. This suggests that clones having an identical chromosome profile also express an identical VAT-repertoire (serodeme).

Animals↗

PubMed1994

Structural analysis and classification of lipocalins and related proteins using a profile-search method.

A three-dimensional profile method of detecting amino-acid sequences compatible with the tertiary structure of any protein has been applied to the lipocalin family of 8-stranded beta-barrels. Profiles derived from six well-resolved lipocalin crystal structures were used to search a comprehensive, non-redundant protein sequence database. Each profile identified a sub-group of lipocalin sequences although no single profile was sufficient to identify the whole family. The alpha-1-acid glycoprotein sub-family was not identified by any lipocalin profile, indicating that known sequence differences in otherwise well conserved regions of these proteins may be reflected in structural differences. The predicted similarity between the beta-lactoglobulin and alpha-2u-globulin structures was much more marked than the similarity between their sequences, and alpha-1-microglobulin sequences were found to be compatible with the structure of epididymal retinoic acid binding protein which has an additional long C-terminal helix. Proteins of unknown structure which were predicted to be compatible with the lipocalin fold include a human mucin. In cases where a large protein family of low overall sequence similarity contains a small number of known structures, this technique can be useful in determining or confirming subtle structural relationships between family members.

Amino Acid Sequence↗

PubMed1984

Density profile and cholesterol concentration of serum lipoproteins in experimental animals and human subjects on hypercholesterolaemic diets.

The density profile of Sudan black stained serum lipoproteins was studied in human subjects and various animal species on diets supplemented with cholesterol. In the animals studied (rabbits, calves, mice, chickens, rats and guinea-pigs), the feeding of cholesterol resulted in an elevation of serum cholesterol levels together with marked changes in the density profile and the cholesterol concentration of the serum lipoproteins. Large differences between animal species in their response to dietary cholesterol were found. In a human subject, an increased concentration of serum cholesterol due to the consumption of a diet supplemented with six egg yolks per day was reflected in an elevated level of LDL cholesterol, while changes in the density profile of stained serum lipoproteins were not observed. In subjects with familial type III and type IV hyperlipoproteinaemia, marked differences in the density profile of lipoproteins were found when compared with that of normolipoproteinaemic subjects. The density profile of stained lipoproteins in the type III patients was remarkably similar to that in cholesterol-fed chickens and lean Zucker rats.

Adult↗

PubMed1991

Substance P immunoreactivity in the rat interpeduncular nucleus: synaptic interactions between substance P-positive profiles and choline acetyltransferase- or glutamate decarboxylase-immunoreactive structures.

The subnuclear and synaptic distribution of substance P immunoreactivity was examined in the rat interpeduncular nucleus at the light and electron microscope level. The nucleus possessed a prominent substance P-immunoreactive axonal plexus in the lateral and dorsomedial subnuclei, and in the dorsal cap of the rostral subnucleus. The density of substance P-immunoreactive axons in the remaining subnuclear divisions was sparse to moderate. Terminals of immunoreactive axons contained spherical vesicles and formed asymmetric contacts on dendritic processes exclusively. Immunoreactive neurons, restricted to the rostral subnucleus, possessed long, sparsely branched dendrites. Unlabelled terminals containing either spherical or pleomorphic vesicles contacted substance P-immunoreactive dendritic profiles. Axodendritic and axosomatic synapses containing substance P immunoreactivity pre- and postsynaptically were not observed. Ultrastructural evidence for synaptic relationships between substance P-containing profiles and those containing either choline acetyltransferase or glutamate decarboxylase was obtained by means of double antigen immunohistochemistry. Terminals of fasciculus retroflexus axons stained for choline acetyltransferase immunoreactivity formed asymmetric synaptic contacts with substance P-immunoreactive dendritic profiles. Few substance P-positive dendrites in the rostral subnucleus received terminals possessing glutamate decarboxylase activity. Unlabelled terminals containing either spherical or pleomorphic vesicles contacted substance P- and glutamate decarboxylase-immunoreactive dendritic profiles simultaneously. Terminals possessing either substance P or glutamate decarboxylase immunoreactivity formed synaptic contacts with dendritic processes of neurons in the lateral subnucleus. Many of the neurons within this subnuclear division contained glutamate decarboxylase. This study provides direct evidence of synaptic relationships between choline acetyltransferase-immunoreactive axons and substance P-immunoreactive dendritic profiles, and between substance P-positive axons and glutamate decarboxylase-immunoreactive dendrites. These findings reveal that two types of transmitter-specific axons of the fasciculus retroflexus innervate neuronal populations of the interpeduncular nucleus stained immunohistochemically for either substance P or glutamate decarboxylase.

Animals↗

PubMed1981

The drug use profile (DUP): an instrument for clinical and research evaluations for drug use patterns.

A profile form, similar to that used by the Minnesota Multiphasic Personality Inventory (MMPI), was developed to portray overall patterns of drug usage. It is based upon data collected from 3984 respondents which were subsequently factor analyzed and transformed into standardized T scores. Once developed, the profile was examined for its ability to evaluate both individual and group drug use patterns. Results suggest that the profile is reliable and provides a quick portrayal of clinically important features of a person's drug practices. Mean drug use data can also be plotted on the same profile for specific subgroups. The profile may be further employed to determine the degree of drug use for a single person or a group of drug users, vis-a-vis the general population. Finally, potential uses for the instrument are discussed.

Adolescent↗

PubMed1996

Expression profiles of active genes in human and mouse livers.

An expression profile of active genes in the human liver was obtained by collecting sequences with a 3'-directed cDNA library that faithfully represents composition of the mRNA population. The results show the relative activity of ca. 600 genes in maintaining the hepatocytes and sustaining their liver-specific phenotypes. The most active group of genes are those for the production of plasma proteins, followed by the genes for the synthesis of lipoproteins, protease inhibitors, coagulation factors, and complements. This balance of gene activity was maintained for four independently obtained expression profiles from human livers, including those of adult and fetus. The expression profiling was extended to the liver of adult mouse, used as a model for the molecular etiology of hepatocytes and for examining the effects of drugs. Subtle biological differences between the human and mouse livers are reflected in the global expression profiles of active genes, especially with regard to the synthesis of plasma proteins, lipoproteins and complements. This comparative analysis using expression profiling should find a wide application in comparative biology.

Adult↗

PubMedDate not supplied

Can dietary macronutrient preference profile serve as a predictor of voluntary alcohol consumption?

A diet rich in protein has been shown to increase voluntary alcohol consumption (VAC) in a variety of animal species. Macronutrient preference (MP) profile varies widely among different outbred rats of the same strain. Using outbred Sprague-Dawley rats and two inbred selected strains of rats (P and NP) that are known for large differences in alcohol preference, we have examined whether 1) there is a relationship between MP profile and VAC and 2) MP profile can be a predictor of VAC. Results of these studies show 1) wide animal-to-animal variations in both the MP and VC and 2) the presence of an association between MP profile and VAC in P and NP but not Sprague-Dawley rats. Therefore, we conclude that MP profile may not serve as a reliable predictor of VAC in a nonselected population.

Alcohol Drinking↗

PubMed1991

Soft-tissue and dentoskeletal profile changes associated with mandibular setback osteotomy.

The aims of the present retrospective cephalometry study were (1) to describe the interrelationships of the soft-tissue and dentoskeletal profiles after total mandibular setback osteotomies and (2) to detect whether there were any cephalometric variables that could contribute to an accurate prediction of the surgical effect on the soft-tissue profile. The presurgical and postsurgical lateral cephalograms of 50 consecutively treated patients (37 females and 13 males) were used; these patients had received combined orthodontic-surgical management of mandibular prognathism by means of a bilateral vertical ramus osteotomy with an extraoral approach. At the time of surgery, their ages ranged from 17 to 41 years. Lateral cephalograms with the teeth in habitual occlusion taken before and approximately 1 year after surgery were available for all patients. A computerized cephalometric appraisal, named profile analysis, was developed and used, including variables corresponding to sagittal and vertical relationships of skeletal and soft-tissue profiles, incisal relationships, soft-tissue thickness, and lip morphology. The statistical elaboration of the data was made by means of paired t test, Pearson's product-moment coefficient correlation, and multiple regression analyses. The assessment of the results disclosed that considerable facial changes and improvement took place after the surgical procedure. The skeletal and soft-tissue facial profiles were straightened and the posture of the lips was improved. The normal incisal relationship achieved became influential on the soft tissues overlying both incisors and led to a better lip competence and posture. Posterior movement at points B and Po was accompanied by reductions ranging from 91% to 103% of the corresponding soft tissues. The presence of both significant correlation coefficients (p less than 0.05) and high r square values (greater than 0.70) in the multiple regression analysis for the osseous tissue variables N-B and N-Po, alone or together with the overjet and S-N-B angle, respectively, reflected their high prediction value with regard to the sagittal relationship of the lower lip, its thickness, and the soft-tissue thickness at the chin area. The findings of Pearson's product-moment coefficient correlation also indicated that the operative changes of the thickness of the upper lip, the lower lip, and the soft tissue at the chin region are influenced by the initial preoperative thickness of the area.

Adult↗

PubMed1992

A comparison of menstrual cycle profiles of salivary progesterone in British and Thai adolescent girls.

Menstrual-cycle profiles of salivary progesterone concentration, obtained by radioimmunoassay of daily samples collected throughout the cycle, were obtained from Thai (n = 232) and British (n = 130) adolescent girls up to 4 years postmenarche. These profiles were graded from 1 to 5 ranging, respectively from concentrations at the detection limit of the assay to profiles generally observed for the mature premenopausal woman. Contingency table analysis of the grade frequencies for Thai-British pairs of girls matched for chronological age and age at menarche (n = 2 x 90) demonstrated that British girls had more mature cycles (22/90) than Thais (11/90) (P less than 0.05) particularly in the first 2 years postmenarche (P less than 0.01). For these matched pairs of girls there was no evidence to support the view that girls with an early age of menarche develop their profiles more quickly following menarche than those with a late age of menarche, as previously reported and which was thought to be important in the development of breast cancer. The findings of this study also suggest that adolescent girls in Britain develop their menstrual cycle profiles of salivary progesterone more quickly than their Thai counterparts and this may be of value in formulating hypotheses regarding any role that ovarian progesterone secretion may have on subsequent breast cancer risk.

Adolescent↗

PubMed2006

Change in lipid profile in celiac disease: beneficial effect of gluten-free diet.

PURPOSE: Celiac disease is associated with hypocholesterolemia, which is thought to contribute to a favorable cardiovascular risk profile. This led to suggestions that the diagnosis of celiac disease and its treatment with a gluten-free diet may result in elevation of the serum cholesterol level and worsen this risk profile. However, no study proves this in adults. We therefore examined the effect of a gluten-free diet on the lipid profile in patients with celiac disease. SUBJECTS AND METHODS: We identified 132 patients with celiac disease who adhered to a gluten-free diet and had lipid profiles performed before and after a median of 20.5 months on the diet. The patients lacked diseases that may affect serum lipids. RESULTS: There were significant increases in total cholesterol and high-density lipoprotein (HDL) cholesterol (P < .0001) but not low-density lipoprotein (LDL) cholesterol (P=.06). The LDL/HDL ratio decreased by 0.36+/-0.7 (P < .0001). Both men and women had a significant increase in total cholesterol and HDL and a significant decrease in the LDL/HDL ratio. Only men had increases in LDL (P=.02). The greatest increase in lipid values was seen in those with the lowest initial values. The largest increase in HDL was seen in subjects with more severe disease as indicated by low albumin level and presence of total villous atrophy. CONCLUSIONS: Diagnosis of celiac disease and its treatment with a gluten-free diet resulted in improvement in the lipoprotein profile, which included an increase in HDL and a decrease in the LDL/HDL ratio.

Adult↗

PubMedDate not supplied

Alterations of lipid profile in endurance over-trained subjects.

BACKGROUND: We tested the hypothesis that endurance over-training could alter the favorable effects of well-tolerated training on lipid profile. METHODS: At weeks 1, 6, 15, 26, 36, and 47 of the training program, blood was drawn to test lipid profile of 20 endurance-trained rowers. Diet and caloric intake were controlled. RESULTS: Over-training was diagnosed in five subjects (loss of performance, asthenia, sleep disturbance...) at week 15 and lipid profile of well-trained and over-trained subjects were compared. Training improved cholesterolemic profile and lowered insulin resistance (HOMA-IR: -39 +/- 9%; p=0.02), and triglycerides concentration (-30 +/- 6%; p=0.05) in rowers who did not change to demonstrate over-training. Plasma LPL (+29 +/- 11%; p=0.01) and hepatic lipase (+5 +/- 3%; p=0.01) activities increased in this group suggesting higher TG utilization and turnover. After week 15 and regarding the well-trained condition for the five over-trained subjects, VLDL-TG (-13 +/- 7 %; p=0.03) and Apo-C(3) (-31 +/- 13%; p=0.01) concentrations decreased, while insulin resistance (+17 +/- 7%; p=0.03) and glycerol concentration (+17 +/- 3%; p=0.01) increased and hepatic lipase activity decreased (-14 +/- 4%; p=0.01). CONCLUSION: Over-training was accompanied by alterations in the lipid profile, which appeared to be the consequence of over-training.

Adult↗

PubMed2006

Unfavorable cardiovascular risk profiles in untreated and treated psoriasis patients.

Psoriasis is a chronic inflammatory skin disease that is associated with an increased cardiovascular risk profile. The systemic inflammation present in psoriasis, various systemic treatments for psoriasis and an increased prevalence of unhealthy life style factors may all contribute to this unfavorable risk profile. The purpose of this article is to provide an overview of what is known about these risk factors in psoriasis, the way they influence the cardiovascular risk of psoriasis patients, and what can be done to reduce this risk. Genetic studies demonstrate that psoriasis and cardiovascular disease share common pathogenic features in which, for example inflammatory cytokines like TNF-alpha and IL-1 play an important role. The chronic inflammation in psoriasis has an unfavorable effect on the cardiovascular risk profile. Multiple cardiovascular risk factors seem to be influenced; the blood pressure, oxidative stress, dyslipidemia, endothelial cell dysfunction, homocysteine levels and blood platelet adhesion. Moreover, classic cardiovascular risk factors like smoking and obesity that have an increased prevalence among patients with psoriasis, indirectly also worsen the cardiovascular risk profile by stimulating the psoriasis activity. Systemic treatments in psoriasis reduce the cardiovascular risk by diminishing the inflammation, but it should be taken into account that most therapies also have adverse cardiovascular effects like dyslipidemia, hyperhomocysteinemia and hypertension. As a consequence preventive measures may be indicated at least during long-term treatments. Prospective research is warranted to accurately estimate the increased cardiovascular risk in psoriasis, to determine the underlying processes and to consider preventive measures according to the absolute risk of cardiovascular disease. The present overview provides data to advice health care providers to pay more attention to the cardiovascular risk profile in psoriasis patients.

Cardiovascular Diseases↗

PubMed2004

Profile analysis of ambient and source emitted particle-bound polycyclic aromatic hydrocarbons from three sites in northern Greece.

Polycyclic aromatic hydrocarbons (PAHs) adsorbed to ambient PM(10) were determined at three sites in Thessaloniki, northern Greece, during the period June 1997-July 1998. Ambient PAH profiles exhibited significant seasonal and spatial variations. Source PAH profiles were obtained for a number of urban, industrial and geological sources including cement, fertilizer and asphalt production, quarry operations, metal electroplating, metal welding and tempering, steel manufacture, lead and bronze smelters, metal scrap incineration, oil burning, non-catalyst equipped passenger cars, diesel fueled taxies and buses, paved road dust and soil dust. Principal component analysis (PCA) and diagnostic ratios were employed to compare ambient and source PAH profiles in an attempt to recognize compositional patterns. Similarities between the ambient PAH profiles and the profiles of certain sources, such as vehicular emissions, oil burning and metal industries, were identified.

Air Pollutants↗

PubMed2003

Fingerprint profiling of acid hydrolyzates of polysaccharides extracted from the fruiting bodies and spores of Lingzhi by high-performance thin-layer chromatography.

Modern extraction and planar chromatographic instrumentation were employed for the fingerprint profiling of carbohydrates from an important and popular medicinal mushroom commonly known as Lingzhi. For the first time, the feasibility of employing the high-performance thin-layer chromatography (HPTLC) peak profiles (fingerprints) of carbohydrates for the screening of various Lingzhi species/products was demonstrated. An analytical procedure was developed such that upon acid hydrolysis of the polysaccharides extracted from various Lingzhi samples, fingerprint profiles that reveal the relative amounts of the degradation products, such as mono- and oligosaccharides, can be obtained using HPTLC plates (Si 50000) for separation and 4-aminobenzoic acid as the post-chromatographic derivatization reagent for detection. Also, using automated multiple development (AMD), the acid hydrolyzates from Lingzhi, consisting of simple and more complex sugars, can be separated simultaneously with high degree of automation. An important finding was that unique fingerprint patterns were observed in the monosaccharide profiles between two highly valued Lingzhi species, Ganoderma applanatum and Ganoderma lucidum, under total or partial acid hydrolysis conditions. Additionally, the HPTLC fingerprint profiles of carbohydrates were obtained from the extracts of the spores and fruiting bodies of Lingzhi and compared.

Acids↗

PubMed2006

A tree-based decision rule for identifying profile groups of cases without predefined classes: application in diffuse large B-cell lymphomas.

In this paper, we examined the utility of a forward growing classification tree as a supplement to cluster analysis for deriving a decision rule for the identification of profile groups when the cases do not belong to predefined classes. The technique was applied for the identification of low and high proliferation profile groups of diffuse large B-cell lymphomas according to the immunohistochemical expression levels of proliferation proteins. In a forward growing classification tree method, the size of the tree is controlled by the improvement (threshold value) in the apparent misclassification rate after each split. The classes used in the tree were defined using k-means clustering. The decision rule consisted of the splitting points of the split variables used. The methodology was applied to the histology data from 79 cases of diffuse large B-cell lymphomas. Ten classes of individual cases were derived from k-means clustering. Then, a classification tree with a threshold of 2% was used to derive the decision rule. Branches at the left side of the tree consisted of individuals with a low proliferation profile and branches at the right side of the tree consisted of cases with a high proliferation profile. The classification tree, as a supplement method, not only identified but also provided decision rules for identifying profile groups. Finally, it also allowed for exploration of the data structure.

Algorithms↗

PubMed2006

Linear regression modeling to compare fluoride release profiles of various restorative materials.

OBJECTIVES: The aim of this study was to compare the released fluoride profiles of various restorative materials by using linear regression analysis. METHODS: Specimens were prepared using a cylindrical Teflon mold with a height of 2 mm and a radius of 8 mm. After being prepared, specimens were immediately placed into artificial saliva which was replaced at various times during 6 weeks. These released intrinsic fluoride amounts were measured by using an ion selective electrode. Then, data obtained cumulatively were statistically analyzed, and the released profiles were compared. RESULTS: It was observed that the materials released fluoride at different levels of concentration and the largest fluoride release was obtained from the conventional glass ionomer cement. This was followed by resin modified glass ionomer cement, polyacid modified composite resin, and fluoride releasing composite resin, respectively. Although the released fluoride amounts of the materials were different, their release profiles were found to be similar in that the release was initially fast and then it became steady as time passed. SIGNIFICANCE: The statistical modeling of the release profiles helps to compare the fluoride release behavior of materials and also to predict fluoride release amounts for the future. In literature, for these purposes, separate nonlinear statistical models have extensively been utilized. However, the single linear statistical modeling approach has numerous advantages such as providing estimators having good statistical properties, exact results, precise inference and simplicity in calculation. Therefore, this study was conducted to introduce the use of single linear regression modeling to compare release profiles statistically.

Compomers↗