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PubMed1987

Vapor pressure isotherms, composition and density of hyperdense bones of horse, whale and porpoise.

Water adsorption studies of three types of hyperdense bone show vapor pressure isotherms and enthalpy to be different from that for pure apatitic mineral. The mineral content of these bones varied from 80 to 98%, much greater than for the better known cortical bone. The high mineral content of all these bones and the variation of mineral content from one type of bone to the next make it possible to evaluate the contribution of the mineral component in bone to water adsorption. Both water content and the fractional shrinkage when dried decrease with increasing mineral content of the bone and increase with the organic component. The total water content of hyperdense bone is much less than the maximum water adsorbed by the equivalent pure mineral powder or by anorganic bone. It was concluded that: (1) Very little water appears to be adsorbed by the mineral in hyperdense bone; (2) It is likely that the mineral crystallites are coated with noncollagenous organic matter; (3) Water is taken up by organic matter both intra- and extrafibrillar; and (4) the enthalpy associated with the adsorption of water by HAP is higher than for hyperdense bone at all corresponding vapor pressures.

Adsorption↗
PubMed1982

Antibodies to sperm-whale myoglobin evoked by free synthetic peptides of an antigenic site.

Previous studies from this laboratory have resulted in the determination of the entire antigenic structure of myoglobin (Mb). The present work was carried out to investigate the antigenicity of the synthetic antigenic sites of Mb in their free form (i.e. without coupling to a carrier) and the effect of peptide size on antigenicity. Site 5 (peptide 145-151) and the longer peptides 145-153, 143-153 and 132-153, each of which carries within it the region of site 5, were synthesized. Immunization of three different mouse strains with each of these peptides, in their free form, resulted in the formation of antibodies that bound specifically to intact Mb. The advantages and significance of these findings are discussed.

Amino Acid Sequence↗