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PubMed1995

Photodynamic action increases leakage of the mitochondrial electron transport chain.

Rat liver submitochondrial particles were treated with rose bengal or flavin mononucleotide and irradiated with visible or near UV light. In both cases, oxygen consumption is impaired after irradiation while O2-. production increases significantly. This suggests that exogenous or endogenous photosensitizers can induce more electron leakage in the mitochondrial respiratory chain. Photobiological implications in dermatology are discussed.

Animals↗
PubMed1994

Involvement of essential histidine residue(s) in the activity of Ehrlich cell plasma membrane NADH-ferricyanide oxidoreductase.

The existence of histidine residue(s) implicated in the active site of NADH-ferricyanide oxidoreductase in plasma membrane vesicles isolated from Ehrlich ascites tumour cells is investigated. The shape of the pH-dependence curve of the enzyme activity suggests that one or more histidine residues are located at (or near) the active site of the enzyme. This hypothesis is supported by the following experimental data: the loss of activity after treatment with diethyl pyrocarbonate (DEPC) or photooxidation by using Rose bengal, and the strong inhibition caused by Zn2+ ions at micromolar concentrations. The combined arguments support the statement that histidine plays an essential role in the catalytic activity of NADH-ferricyanide oxidoreductase from Ehrlich ascites tumour cells.

Animals↗
PubMed2006

Photooxidation of acyclovir with thermally generated triplet excited ketones. A comparison with type I and II photosensitizers.

The antiviral drug acyclovir (Ac, 1) was treated with triplet excited ketones, which have been generated in thermal decomposition of 3-(hydroxymethyl)-3,4,4-trimethyl-1,2-dioxetane (HTMD), in the dark. Three major oxidation products were detected by means of spectroscopic measurements. The products were (2-hydroxyethoxy) methyl spiroiminodihydantoin (2), (2-hydroxyethoxy) methyl (amino)-2-imino-1,2-dihydroimidazole-5-one (3), and 2,2-diamino-4-[(2-hydroxyethoxy) methyl) amino)-5-[2H]-oxazolone (4). Equal amounts of type I and type II photooxidation products were found, as could be established by comparison with predominant type I (riboflavin) and type II (rose bengal) photosensitizers. The concentration and time profiles for the HTMD-induced oxidation of Ac were also determined. The participation of singlet oxygen in HTMD-induced oxidation was confirmed by the substantial D(2)O effect in the formation of spiroiminodihydantoin (2).

Acyclovir↗
PubMed1976

Absorbed radiation dose from radionuclidic impurities in several 123I-labeled radiopharmaceuticals.

The radiation dose to patients from RISA, sodium iodohippurate, and rose bengal labeled with commercially available 123I and its accompanying radioimpurities has been studied. The extra radiation dose to target organs was found to range from 29% to 97% that due to 123I alone when the radiopharmaceutical is administered at calibration time. Additional delay in the time of administration increases the relative impurity dose.

Drug Contamination↗
PubMed1997

Singlet oxygen mediates the activation of JNK by UVA radiation in human skin fibroblasts.

Ultraviolet A (UVA: 320-400 nm) radiation activates c-Jun-N-terminal kinase (JNK 2) in human skin fibroblasts. Exposure of cells to UVA (300 kJ/m2) led to a 5-fold induction of JNK-activity which was significantly increased in the presence of D2O, an enhancer of the lifetime of singlet oxygen. Sodium azide, a quencher of singlet oxygen, abolished the activation of JNK. A hydroxyl radical scavenger, mannitol, had no effect. Furthermore, photochemically produced singlet oxygen (Rose Bengal plus white light) was found to induce JNK activity. This was enhanced by D2O and inhibited by azide. Thus, singlet oxygen activates and mediates the UVA-induced activation of JNK.

Calcium-Calmodulin-Dependent Protein Kinases↗
PubMed1981

Brucellosis in free-living African buffalo (Syncerus caffer): a serological survey.

The rose bengal and complement fixation tests were successfully applied to buffalo (Syncerus caffer) sera. An overall occurrence of 23% positive reactors was obtained, but Brucella infection does not appear to act as an effective culling agent in buffalo. Any eradication programme must take free-living buffalo into account as a possible source of re-infection for cattle.

Agglutination Tests↗
PubMed1996

[A laboratory method for sensitized isomerization of polyene macrolides].

The study was aimed at the development of a laboratory procedure for isomerization of polyene macrolides in the presence of sensitizers. The reaction was performed with exposure of levorin solutions to the xenon lamp irradiation in the presence of rose bengal B as a sensitizers. It was shown that levoristatin contain in the levorin sample was not degraded under the experimental conditions. The use of a redetermined variant of the Vierordt's methods made it possible to offer a design formula for the estimation of the isolevorin content in solutions in the presence of levorin.

Anti-Bacterial Agents↗
PubMed1992

Identification of an essential histidine residue at the active site of the tonoplast malate carrier in Catharanthus roseus cells.

The involvement of a histidyl residue in the binding or translocation step was investigated in the malate carrier at the tonoplast of Catharanthus roseus cells. The transport rate was strongly stimulated when the pH of the incubation medium was decreased from pH 7.0 to 5.0. The histidine-specific reagent diethylpyrocarbonate (DEPC) efficiently inhibited the activity of the malate carrier. Inhibition developed rapidly and was completed after 5 min at a concentration of 2 mM DEPC. The original substrate, malate, partially protected the carrier from inactivation by DEPC. Other organic acids (citrate, quinate) which are known to affect the malate transport of isolated vacuoles or tonoplast vesicles also showed protective properties. Inhibition of malate transport on tonoplast vesicles can also be achieved by photooxidation in the presence of the dye Rose Bengal. Malate also proved to protect against inactivation. The results strongly support the notion that a histidyl residue(s) is involved either in the binding or translocation of malate and that the protonation of the histidyl residue is essential to provide a high rate of malate transport.

Biological Transport↗
PubMed2005

Specificity dependence between serological tests for diagnosing bovine brucellosis in Brucella-free farms showing false positive serological reactions due to Yersinia enterocolitica O:9.

When brucellosis false positive serological reactions happen in cattle, the serial use of pairs of specificity-correlated serological tests (rose bengal, complement fixation, competitive ELISA) results in specificities lower than expected. In this situation, highly specific tests, such as the indirect ELISA used alone, may be more adequate than serial testing.

Agglutination Tests↗
PubMed1988

Inhibition of the calcium channel by intracellular protons in single ventricular myocytes of the guinea-pig.

1. The inhibitory effects of intracellular protons (Hi+) on the L-type Ca2+ channel activity were investigated in single ventricular myocytes of guinea-pigs by using the patch-clamp method in the open-cell-attached patch configuration, where 'run down' of the channel was partially prevented. 2. Hi+ reduced the unitary Ba2+ current of the Ca2+ channel by 10-20% without changing the maximum slope conductance. 3. Hi+ did not alter the number of channels in patches containing one or two channels. 4. Hi+ markedly reduced the mean current normalized by the unitary current, which gave the open-state probability multiplied by the number of channels in the patch. The dose-response curve between Hi+ and the open-state probability indicated half-maximum inhibition at pHi 6.6 and an apparent Hill coefficient of 1. 5. Hi+ shifted both the steady-state activation and inactivation curves in a negative direction by 10-15 mV, and the effects were reversible. 6. Hi+ did not affect the fast open-closed kinetics represented by the C-C-O scheme, apart from increasing the slow time constant of the closed time. 7. Hi+ increased the percentage of blank sweeps and reduced that of non-blank sweeps resulting in a decreased probability of channel opening. 8. Photo-oxidation with Rose Bengal abolished the reducing effect of Hi+ on the open-state probability (Po) in two out of ten experiments, suggesting the possible involvement of histidine residues in the Hi+ effect. 9. The above results indicate that Hi+ inhibits the Ba2+ current mainly by affecting the slow gating mechanism of the channel.

Action Potentials↗
PubMed1997

Soluble interleukin-2 receptors and serum autoantibodies in dry eye patients: correlation with lacrimal gland function.

PURPOSE: Sjögren's syndrome (SS) is a systemic autoimmune disease characterized by decreased lacrimal and salivary gland function causing dry eye and dry mouth. We examined several parameters as measures of lacrimal function and compared the serum soluble interleukin-2 receptor (sIL-2R) and serum autoantibodies as measures of systemic autoimmune condition. METHODS: Serum levels of sIL-2R, antinuclear antibody (ANA), and rheumatoid factor (RF) in 128 patients (16 men, 112 women; average age, 54.3 +/- 12.5 years) were measured. The lacrimal function was evaluated clinically. RESULTS. There was no correlation between systemic condition and the cotton-thread test, Schirmer test with or without anesthesia, tear-clearance test, tear-function index, or the BUT. However, the Schirmer test with nasal stimulation and rose bengal and fluorescein staining was positively correlated with systemic parameters. CONCLUSION: We show the correlation of serum sIL-2R, ANA, and RF to lacrimal function, which is good evidence that systemic immune conditions directly affect local lacrimal gland function.

Antibodies, Antinuclear↗
PubMed1991

Congenital alacrima without associated manifestations (AD). An affected father and son.

The authors report a Kuwaiti Arab family in which the father and one of his two sons have severe hypolacrimation with blotchy staining of the cornea and punctate staining of the interpalpebral bulbar conjunctiva by fluorescein and Rose Bengal. Pharmacologic testing together with biochemical analysis and systemic examinations and investigations suggest an isolated dysfunction of lacrimation. The authors' small Arab family differs from the only other recorded pedigree (Irish) in which all five affected members in four generations also had atopy.

Adult↗
PubMed1969

Transient changes in extrinsic fluorescence of nerve produced by electric stimulation.

NERVES STAINED WITH ONE OF THE FOLLOWING FLUOROCHROMES WERE FOUND TO RESPOND TO ELECTRIC STIMULATION WITH TRANSIENT CHANGES IN THE FLUORESCENT LIGHT: acridine orange, acridine yellow, 8-anilinonaphthalene-1-sulfonate, auramine O, 1-dimethylaminonaphthalene-5-sulfonyl chloride, fluorescein isothiocyanate, LSD, pyronin B, pyronin Y, rhodamine B, rhodamine G, rose bengal, and 2-toluidinylnaphthalene sulfonate. The properties of covalently bonded fluorochromes in the nerve and the behavior of fluorochromes introduced into the axon interior were studied. By measuring fluorescence polarization, direct evidence was obtained showing a decrease in intramembrane viscosity during the action potential.

Acridines↗
PubMed2000

The use of vital dyes in corneal disease.

Vital dyes such as fluorescein, rose bengal, and lissamine green B, among others, have been an important tool in clinical ophthalmology in diagnosis and evaluation of various ocular surface disorders. Each dye, having a unique chemical structure, has distinct characteristics that allows its preferential use in different ocular pathologies. In this article, history of vital dyes and the use of popular current vital dyes in corneal disease are reviewed.

Corneal Diseases↗
PubMed1976

A quantitative comparison of the sensitivity of serological test for bovine brucellosis to different antibody classes.

Brucella-specific antibodies of different immunoglobulin classes were quantitatively evaluated with respect to their efficiency in serological tests for bovine brucellosis. IgM reacted more efficiently than IgG1 and IgG2 in both the Rose Bengal plate test and serum agglutination test. The complement fixation test was found to be slightly more sensitive to IgM than to IgG1 and did not react to IgG2. IgM was, however, partly inactivated when heated at 60 degrees C. in the presence of serum.

Agglutination Tests↗
PubMed2006

Atelocollagen punctal occlusion in dry eye patients.

PURPOSE: To evaluate the efficacy and safety of atelocollagen punctal occlusion for dry eye patients. DESIGN: Prospective noncomparative interventional case series. METHODS: Atelocollagen was injected into the superior and inferior canaliculi of 52 eyes of 28 dry eye patients. Vital staining of the ocular surface, breakup time of tears (BUT), tear volume, and corneal epithelial permeability to fluorescein were examined before and 1, 2, 4, and 8 weeks after treatment. RESULTS: Rose bengal stain, fluorescein stain, BUT, and corneal epithelial permeability to fluorescence were significantly improved 1 week after atelocollagen punctal occlusion, and the improvement was maintained for up to 8 weeks after treatment. CONCLUSIONS: Atelocollagen punctal occlusion effectively improves ocular surface disorders in dry eye patients.

Adult↗
PubMed1982

A study of cattle infected with Brucella abortus and which showed aberrant serological reactions.

Sixty cows, 48 of which had been vaccinated with live Brucella abortus strain 19 (S19) or with killed B. abortus strain 45/20 (S45/20) and 12 of which were unvaccinated animals, were challenged with B. abortus strain 544. Ten of the 27 cattle found to be infected after challenge showed aberrant serological reactions to the Rose Bengal Plate test, serum agglutination test and/or complement fixation test. These 10 cattle were all previously vaccinated with S19 or S45/20. It was concluded that infection in cattle vaccinated with S19 or S45/20 may be more difficult to detect than infection in animals that have no history of vaccination.

Animals↗
PubMed2000

Survey of preferred tests for diagnosis of the tear film and dry eye.

PURPOSE: To survey practitioners in the tear film/dry eye field for their preferred diagnostic methods for the evaluation of the tear film and dry eye syndrome. METHODS: A survey was given to 36 optometrists and 41 ophthalmologists with backgrounds in the area of tear film and dry eye syndrome to find their preferred test if only one diagnostic option was available for tear film evaluation. Second, third, and fourth choices were also recorded, as well as any additional comments. RESULTS: Sixty-eight practitioners completed the survey. No one single test was a dominant first choice of the majority of respondents in either profession. History and/or dry eye questionnaire was the most frequent response to the question addressing the use of only one test (28%). The second most frequently chosen test was fluorescein break-up time (FBUT) (19%), followed by fluorescein staining (13%), and rose bengal (10%). The Schirmer test was most frequently included as one of the four choices (62%), although it was the first choice of only 9%. CONCLUSION: The importance of multiple tests in the evaluation of tear film disorders is overwhelmingly acknowledged, with patient history/dry eye questionnaires, FBUT, ocular surface staining, and the Schirmer test being the preferred diagnostic tools.

Contrast Media↗