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Detection of chlamydia trachomatis antigen using the direct immunofluorescence technique: a four year survey in Northwestern Greece.

The prevalence of Chlamydia trachomatis antigen in patients visiting the University Hospital of Ioannina, North-western Greece during 1991-1993 is surveyed. Six hundred and twenty two (622) samples (scrapings from the cervix, uretra and conjunctiva) were examined for the detenction of C. trachomatis antigen using the direct immunofluorescence technique (DIF). From the 662 samples examined by DIF, 579 were found negative (93%) and 43 positive (7%).

Antigens, Bacterial↗

Canine visceral leishmaniasis in the great Athens area, Greece.

A survey of 1,175 dogs with suspected visceral leishmaniasis (VL), was carried out to investigate canine leishmaniasis in the Athens basin, the largest metropolitan area of Greece. The dogs examined were not a random sample since animals were selected on the basis of symptoms indicating the disease. Dog sera were tested using the indirect fluorescent antibody technique (IFAT), with titres greater than or equal to 1/200 considered positive for VL. On this basis 569 dogs (48.4%) were found positive for VL and 69 (5.9%) borderline (with titres of 1/100). Additionally, 218 of these sera were also tested by enzyme-linked immunosorbent assay (ELISA). Of this sample, 120 were negative and 88 were positive with both IFAT and ELISA (according to the stated criteria), and thus the concordance of the two methods was 98.6%. Dogs positive for VL were classified according to age, sex, breed, and area of residence. Dogs from outlying areas of the Athens basin had higher infection rates than dogs from the city. The most affected breed was the Doberman, and the least affected was the Collie.

Animals↗

[Traditional medicine in ancient Greece (coexistence of Asclepian art and Hippocratic medicine)].

Ancient Greece created two types of medicine: priestly-religious one with god Asclepios and temple treatment and other rational one, formed by Hippocrates. Both coexisting types of medicine represented two non-antagonistic alternatives of treatment for the patients. In spite of differences between both kinds of ancient Greek medicine we have no evidence either of a collaboration between Asclepian priests and Hippocratic physicians, or of any mutual misunderstanding or hostility, Asclepios followers respected the tradition, believed in the divine descent of their profession, but remained loyal to the Hippocratic Oath. The fact, that when the rational medicine did not help the patient, he searched his healing in religious or other alternative type of medicine, happens often even in contemporary times.

Greece, Ancient↗

Typing of Legionella pneumophila strains isolated in Greece by arbitrarily-primed PCR.

Genomic fingerprints from DNA of fifteen environmental and clinical strains of Legionella pneumophila serogroup 1, isolated from diverse geographic areas of Greece, during the period 1986 to 1994, were generated with arbitrarily-primed polymerase chain reaction (AP-PCR) in order to use the discriminatory power of two arbitrary primers, BG2 and M13 Forward to clarify the relationship among the fifteen isolates. Both primers were found to have the ability to discriminate strains of the same serogroup, to identify strains related to each other even though they were isolated at different times. Therefore, AP-PCR using BG2 or M13 Forward as primers, appears to be a useful tool which provides a fast and simple method for epidemiological fingerprinting.

Bacterial Typing Techniques↗

Fatal animal poisonings in northern Greece: 1990-1995.

Fatal animal poisonings in northern Greece from 1990 to 1995 are recorded. A total of 926 specimens have been analyzed by chromatographic techniques. Pesticides caused 78% of the poisoning cases and all other toxic substances caused 22%. The animals affected were mainly cats, dogs, sheep, birds and bees.

Animals↗

Perioperative nursing in Greece.

In Greece perioperative nursing is comprised of preoperative, intraoperative, and postoperative nursing. Nursing care in these phases involves general and presurgical preparation, all activities performed during surgery, and the care given in both the recovery room and clinical unit after surgery.

Greece↗

Aircrew fatigue monitoring during sustained flight operations from Souda Bay, Crete, Greece.

BACKGROUND: Operational flight surgeons are often responsible for determining aeromedical readiness of aircrew members whose accumulated flight time exceeds standard limitations. Realizing that operational reports of excessive flight time in aircrew are limited, we used Rayman's 1975 study of fatigue during Cambodian airlift missions as a model to evaluate 42 U.S. Navy EP-3E aircrew members flying reconnaissance missions from Souda Bay, Crete, Greece. Measured parameters focused on information accessible to operational flight surgeons. METHODS: Hoping to identify early indices of fatigue, the aircrews were monitored using anonymous questionnaires, physiologic data (mean arterial pressure, pulse, pulse pressure), and hematologic measurements (CBC, sedimentation rate). RESULTS: As suspected, no physiologic parameter indicated early fatigue. However, some aircrew demonstrated small changes in measured visual phorias as compared to prior evaluations. Anonymous questionnaires and subjective evaluation of crewmembers appeared most valuable in assessing fatigue. CONCLUSION: Incorporating previously reported recommendations for fatigue surveillance, the Souda Bay experience is an example of successful fatigue monitoring in aircrews who accumulate flight time beyond standard restrictions.

Adult↗

Factors associated with back pain in nursing staff: a survey in Athens, Greece.

Factors associated with self-reported back pain were investigated using questionnaire data on 407 female nurses from a large hospital in Athens, Greece. Factor analysis was used to construct indices of pain and its impact on normal life, and also to summarize work load descriptions. Pain factors were examined in relation to work load and personal characteristics by logistic regression. Statistically significant items, in relation both to pain and impact, were the existence of previous back injury, self-reported headaches and the 'carrying and lifting' factor of work load which principally included moving equipment. Age, height and weight were not associated with back pain. Compared to a similar study by Harber and colleagues in California, United States of America, the factors associated with back pain were quite similar in this study even though the prevalence of back pain was much higher among Greek nurses and their work load was physically much more demanding.

Back Pain↗

Informant-rated activities-of-daily-living (ADL) assessments: results of a study of 141 items in the U.S.A., Germany, Russia, and Greece from the International ADL Scale Development Project.

The analyses of an international pilot study presented in this article focused on the development of a cross-nationally valid activities-of-daily-living (ADL) scale sensitive to therapeutic effects in patients with mild memory impairment and mild to moderately severe dementia. The present report, which is part of an ongoing international research project, describes field testing results for 141 informant-rated items. The comparability of samples investigated in research centers in the U.S.A., Germany, Russia, and Greece concerning cognitive decline was evaluated globally as well as psychometrically using the Global Deterioration Scale, the Short Cognitive Performance Test, and the Mini-Mental State Examination. In the participating countries, a composite ADL score discriminated well between different stages of cognitive impairment because of dementia. However, international differences between the applied measures were observed. A practical ADL scale showing therapeutic sensitivity and international utility, will be constructed from the 141 informant-rated items under investigation in this pilot work.

Activities of Daily Living↗

Seasonal variation of the abdominal aortic aneurysm rupture in southwestern Greece.

OBJECTIVE: To examine the hypothesis of seasonal variation in the rupture of abdominal aortic aneurysm in our region. DESIGN: Retrospective open study. SETTING: University Hospital, Greece. PATIENTS: Forty-six patients with abdominal aortic aneurysm admitted between 1991-1995. INTERVENTION: The month of admission was registered. MAIN OUTCOME MEASURES: The seasonal variation in the abdominal aortic aneurysm rupture. RESULTS: The majority of ruptures (78%) occurred during the months October and April, a phenomenon proven to be periodical (p<0.05). CONCLUSIONS: The abdominal aortic aneurysm rupture clearly showed seasonal variation.

Aged↗

Prevalence and sensitivity to antibiotics of Enterobacteriaceae isolated from urinary cultures in some microbiology laboratories of a city in west Greece.

The prevalence of Enterobacteriaceae from midstream urine samples from patients with community acquired urinary tract infections (UTI) of a town in SW Greece during one year period and their susceptibility to antibiotics were studied. The most frequently recovered pathogens were E. coli (77%), Proteus mirabilis (10%), Klebsiella spp (8.7%), Enterobacter spp (2.5%) and Citrobacter freundii (1.8%). E. coli were found more resistant to carbenicillin, the combination of amoxicillin/clavulanic acid and cotrimoxazole. Half of the strains were found resistant to more than one antibiotics. All strains were found sensitive to aminoglucosides, 2nd generation cephalosporines (except cefoxitin), 3rd generation cephalosporines, aztreonam and imipeneme. According to our results a statistically significant increase of the resistance to antibiotics at individuals over 45 years of age was noticed. The positivity of the samples was not correlated to prior antibiotic consumption and to the occupation of the participants or their residence.

Adolescent↗

Selenium content of sulfide ores related to ophiolites of Greece.

Several deposits of sulfide mineralization have been described in the ophiolites of Greece. Based on their mineralogical and chemical composition and the host rocks, two types can be distinguished: (1) the Fe-Cu-Ni-Co type consisting of pyrrhotite, chalcopyrite, Co-pentlandite, pyrite, magnetite + arsenides, +/- chromite, hosted in serpentinites, gabbros or diabases, which have variable geochemical characteristics, and (2) sulfide mineralization of the Cyprus type containing variable proportions of pyrite, chalcopyrite, bornite, and sphalerite. The spatial association with shear zones and fault systems, which is a common feature in both types of mineralization, provided the necessary permeability for the circulation of the responsible mineralized hydrothermal fluids. The selenium (Se) content in representative samples of both types of mineralization from the ophiolites of Pindos (Kondro, Perivoli, and Neropriona), Othrys (Eretria and A. Theodoroi), Veria (Trilofon), and Argolis (Ermioni) shows a wide variation. The highest values of Se (130 to 1900 ppm) were found in massive Fe-Cu sulfide ores from Kondro, in particular the Cu-rich portions (average 1300 ppm Se). The average values of Se for the Othrys sulfides are low (< 40 ppm Se). The Se content in a diabase breccia pipe (50 x 200 m) with disseminated pyrite mineralization (Neropriona) ranges from < 1 to 35 ppm Se. The highest values were noted in strongly altered samples that also exhibited a significant enrichment in platinum (1 ppm Pt). Sulfide mineralization (irregular to lens-like masses and stringers) associated with magnetite, hosted in gabbros exposed in the Perivoli area (Tsouma hill), shows a content ranging from 40 to 350 ppm Se. The distribution of Se in the studied type of the sulfide mineralization may be of genetic significance, indicating that the Se level, which often is much higher than in typical magmatic sulfides related to mafic-ultramafic rocks (average 90-100 ppm Se), may positively affect the environment.

Environment↗

Surgery for ulcerative colitis in Greece: clinicoepidemiological features and long-term outcome of 69 consecutive patients.

BACKGROUND/AIMS: In this study, the various clinicoepidemiological characteristics and long-term follow-up of patients operated on for ulcerative colitis in Greece over a period of 15 years, are reviewed. METHODOLOGY: A total number of 69 out of 413 patients with ulcerative colitis (17.8%) seen and followed-up for a mean period of 12 years, had undergone ileorectal anastomosis (45.6%), total proctocolectomy with permanent ileostomy (35.6%), ileal-anal pouch anastomosis (17.6%) and Kock ileostomy (1.5%), mainly for bad response to conservative treatment (78.3%). Other causes for operation were large bowel cancer (8.7%), profound hemorrhage (4.3%), toxic megacolon (1.4%), and rectovaginal fistula (1.4%). Almost half of the operations (45%) were performed between the first and fourth year after the establishment of diagnosis. RESULTS: A number of statistically highly significant differences between the operated and non-operated group of patients were noticed. So, patients operated on had more extensive disease in comparison with non-operated ones and were younger at the time of diagnosis. The overall perioperative morbidity and mortality were 6% and 5% respectively. Survival was not statistically significantly different between operated and non-operated patients and also did not differ significantly between men and women. Most of the deaths in the operated and non-operated group of patients were unrelated to ulcerative colitis. CONCLUSION: It is concluded that the clinicoepidemiological features and outcome of the Greek patients operated on for ulcerative colitis have similarities with those reported from other developed countries of the world.

Adult↗

[Medical care for the wounded in armies of ancient Greece].

Discussions of medical treatment in ancient armies usually focus on the medical services in the Imperial Roman army--not least for the reason that we have far more evidence regarding Roman armies than we have for medical treatment in the armies of ancient Greece. It is, however, possible to cull some information from brief remarks scattered throughout Greek literature, medical as well as non-medical. Based on these, it appears possible to confirm that most, if not all, Greek armies would have had several doctors available for the treatment not only of the leaders, but also of the officers and common soldiers.

Greece, Ancient↗

Human and canine dirofilariasis in Greece.

Human dirofilariasis is a disease which is considered to be rare in Greece, less than 20 cases having been reported prior to 1990. The clinical manifestations were usually subcutaneous nodules, and in only two cases there was ocular dirofilariasis. Eight new cases were recognized in humans during the last six years in Athens, two of which with ophthalmic involvement. Twelve additional cases have been detected but not published in Thessaloniki. In all human cases, the worms were identified as being Dirofilaria repens (D. conjunctivae). The identification was based on the morphological observations of the whole nematodes and on the histological sections of the subcutaneous nodules in the samples. Four different filarial nematodes (D. immitis, D. repens, Dipetalonema reconditum and D. grassii) were recovered from dogs. Infection of dogs with all types of filariae ranges from 12% to 37%. The high incidence of dirofilariasis in dogs is of great interest compared to the low incidence in humans. The occurrence of common host animals and insect vectors makes dirofilariasis a potential danger for public health.

Animals↗

The prevalence of goat hypodermosis in Greece.

Goat hypodermosis caused by Przhevalskiana silenus is common in Greece causing quite serious losses on animal production and breeding, but no data regarding the prevalence of this parasitic disease were available within the last 17 years. Towards this end, at the slaughter house of the area of Thessaloniki, the number of affected hides due to skin penetration by P. silenus larvae was recorded and the prevalence was assessed to be 54.2%. The mean number of holes found per hide was 27.07 (ranged from 0 to 162 holes per hide). Moreover, a total of 4900 goat blood serum samples were collected from different parts of the country and tested for hypodermosis using the ELISA method. Based on the results of this test, the prevalence of the infection was found to be 49.2%, varying from place to place between 4.6 and 85.1%. The range of the infection per flock was from 0 up to 100%.

Animal Husbandry↗

The prevalence of bovine hypodermosis in Greece.

Hypodermosis is a common parasitic disease of great importance on cattle production and breeding worldwide. In Greece, no reliable data are available regarding the extent of this infection and therefore, as expected, no national or regional control programme is being applied. In order to cover this lack of information, a total of 4200 cattle blood sera were collected from different parts of the country and tested for hypodermosis using the ELISA method. The prevalence of the infection was found to be 37.4%, varying from place to place between 0.7 and 78.8%. The range of the infection per flock was from 0 up to 100%.

Animals↗