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At least 1,063 records · Page 59Linked to original sources
PubMed1999

Comparison of the growth patterns and morphological characteristics of mechanically and enzymatically isolated fallopian tube epithelial cells.

This study set out to compare the growth patterns and morphological characteristics of human fallopian tube epithelial cells isolated: (1) mechanically; and (2) enzymatically. Cells were cultured in medium supplemented with fetal bovine serum and antibiotics and their epithelial nature was established by immunocytochemistry for cytokeratins. Primary cultures were polygonal in shape with centrally located nuclei, irrespective of the isolation method. Cells isolated enzymatically exhibited a higher growth rate, but the survival rate was poor after more than 2-3 passages. Mechanical isolation gave a lower yield of cells, but had a higher survival rate when sub-cultured, even beyond 8 passages. Thus, mechanically isolated cells might be useful for longer term cultures, whereas enzymatically isolated cells are best only for short-term work.

Adult↗
PubMed1985

Calcitonin gene-related peptide inhibits spontaneous contractions in human uterus and fallopian tube.

The localization and effects of calcitonin gene-related peptide (CGRP) in the human uterus and fallopian tube were investigated. CGRP-immunoreactive nerve fibers were found in the muscular layers, around blood vessels and close to the epithelium. The oviduct and uterine cervix were more densely innervated than the corpus of the uterus. Substance P (SP)-like immunoreactivity was found in nerves with an overlapping distribution to that of CGRP-positive fibers. CGRP (2 X 10(-10) to 10(-7) M) dose-dependently and reversibly inhibited spontaneous contractions of uterine and oviductal strips, as well as SP-induced contractions in the oviduct. A role for CGRP-containing nerve fibers in regulation of motor activity in human female reproductive organs is suggested.

Adult↗
PubMedDate not supplied

Clinical and cytologic aspects of primary fallopian tube carcinoma. A report of ten cases.

The clinical and cytologic findings in ten cases of primary fallopian tube carcinoma, a very rare malignancy, are presented. All ten patients had vaginal pool (V), cervical (C), endocervical (E) and endometrial aspiration (EA) smears examined preoperatively; peritoneal smears were also prepared from cul-de-sac fluid aspirated during surgery. In the preoperative cytodiagnosis, some of V, C, E and EA smears were positive for malignant cells in six of the ten patients (60%). Examination of the EA smear was the most effective means of discovering this disease. A watery discharge was noted in two patients, both of whom were negative in the preoperative cytodiagnosis. In such patients, it is necessary to repeat the smear examinations. The cytologic appearance of this disease is contrasted to that of endometrial carcinoma.

Adult↗
PubMed1991

[Treatment of fallopian tube pregnancies with prostaglandins].

In a study, the clinical use of prostaglandin F2 alpha in local and systemic application in women with ectopic pregnancies were studied (1, 9). Two different treatment schedules were defined and applied. In group A, patients with a diagnosed ectopic and beta-HCG level lower than 850 mIU/ml were treated with prostaglandin F2 alpha i.m. injected only. In group B, prostaglandin F2 alpha were injected in the chorionic cavity of the ectopic by laparoscopy after localisation with a thin needle. In spite of prostaglandin F2 alpha treatment, 6 of 30 patients (20.0%) had to be operated by microsurgery because of increasing serum beta-HCG levels. A control of tubal patency 6 month later showed one closed tube only (4.5%). Up to now, 8 spontaneous intrauterine pregnancies occurred in our study groups after successful prostaglandin F2 alpha treatment; one pregnancy was seen in a women with a single fallopian tube. The conserving treatment of one ectopic pregnancy using prostaglandin F2 alpha yields positive results, if serum beta-HCG levels are below 2000 mIU/ml.

Abortifacient Agents, Nonsteroidal↗
PubMed1991

Isolation and monolayer culture of human fallopian tube epithelial cells.

In the present study we have established a pure monolayer culture system of human fallopian tube epithelial cells. The cells were isolated using collagenase digestion, and were cultured in Medium 199 supplemented with 15% fetal bovine serum. The epithelial cells derived from primary and secondary culture were characterized using immunocytochemical staining and electron microscopy. The cells continued to grow for 2 to 3 wk once the monolayer culture of the cells was established. It is currently possible to maintain the cultures until the third generation. Proliferation of these cells was enhanced by epidermal growth factor but not by basic-fibroblast growth factor, insulin, transferrin, estradiol-17 beta, or progesterone. This culture system offers a good model for determining characteristics of the tubal epithelium and would permit effective study of co-culture with embryos.

Adult↗
PubMed1976

Characteristics of responses of isolated human fallopian tube to transmural stimulation and to sympathomimetic amines.

The responses of circular and longitudinal muscle of human fallopian tube to transmural stimulation and sympathomimetic amines have been investigated. It was found that tissues from the earlier part of the menstrual cycle (i.e., estrogen dominant) were less responsive to all stimuli than those from the middle or late phase of the cycle. Although excitatory alpha responses of ampulla and isthmus could be observed, the predominant response of ampulla and isthmus, in all phases of the menstrual cycle, to (--)-noradrenaline and transmural stimulation was inhibition of spontaneous activity. The inhibitory response to transmural stimulation, was abolished by tetrodotoxin and guanethidine. The inhibitory responses to transmural stimulation and (--)-noradrenaline were reversed by propranolol. These findings show that the response of tissues to transmural stimulation resulted from release of noradrenaline from adrenergic nerves, and subsequent action on inhibitory beta adrenergic receptors.

Electric Stimulation↗
PubMed1983

Menstrual-cycle-dependent and -independent features of the human Fallopian tube fimbrial epithelium: an ultrastructural and cytochemical study.

Ultrastructural and cytochemical features of 100 specimens of human Fallopian tube fimbrial epithelium were studied to determine whether they were dependent on or independent of the ovarian hormones. Characteristics which were independent of hormonal control included the presence of alkaline phosphatase reaction product along the apical cell membrane of the secretory cell and its absence in the ciliary cell. Acid phosphatase reaction product was found in ciliary and secretory cell lysosomes in similar numbers and calcium deposits were observed in ciliary and secretory cell nuclei, nucleoli and cell membranes. On the other hand, characteristics dependent on stages of the cycle included the presence of mitochondrial-bound calcium during the preovulatory phase and its depletion during the periovulatory phase. The same sequence was noticed in the content of the large ciliary cell perinuclear globules, which displayed accumulations of glycogen and lipid in preovulatory phase and their depletion during the periovulatory phase. It seems that during the periovulatory phase the increased metabolic activity of the cells is accompanied by utilization of some of the material stored within the cells.

Alkaline Phosphatase↗
PubMed1998

Primary carcinoma of the fallopian tube: comparative genomic hybridization reveals high genetic instability and a specific, recurring pattern of chromosomal aberrations.

Primary fallopian tube carcinoma (PFTC) is a rare and highly aggressive tumor. Twelve cases of PFTC (stages IA to IV) were analyzed by comparative genomic hybridization. The most consistent DNA gain was mapped to chromosome arm 3q in 11 of 12 cases. In six cases, the gain of 3q was present as a high level copy number increase (amplification) with a consensus region mapped to 3q26.2-qter. In the 12 cases, other frequent gains were located on chromosome arms 1q (in 11 cases), 2q (in 10), 7q (in 9), 8q (in 9), 5p (in 8), 6p (in 7), 12p (in 7), and 14q (in 6). Frequent copy number losses occurred on chromosome arms 16q (in 8 cases), 22q (in7), 6q (in 6). 8p (in 6), 18q (in 6), Xq (in 6), 1p (in 5), and 17p (in 5). All chromosomes were involved in chromosomal aberrations and the average number of copy alterations per case was 19.7. None of the 12 carcinomas revealed the presence of human papillomavirus (HPV) genomes. All of the cases exhibited crude aneuploidy. Strong p53 immunoreactivity could be observed in 10 of 12 cases while p21/WAF1 expression was low or undetectable. These results indicate that PFTC is a genomically highly unstable cancer, an observation that is in agreement with the poor prognosis associated with this tumor. A high frequency of 3q-gains has also been observed in HPV-related carcinomas of the uterine cervix. However, none of the PFTC was HPV related, suggesting that the 3q-gain is independent from HPV DNA.

Aged↗
PubMed1999

Microendoscopy of the human fallopian tube.

Advances in fiberoptic technology make it possible to evaluate the tubal lumen endoscopically. Two basic procedures are salpingoscopy and falloposcopy. The former is a transfimbrial approach allowing visualization from the ampullary-isthmic junction to the fimbriae. The latter is a transcervical approach allowing assessment of the tubal lumen from the uterotubal ostium to the fimbriae. Both may be performed without general anesthesia. The procedures provide more sensitive information than laparoscopy with chromotubation or hysterosalpingography alone. Findings provide a strong correlation with conception. Technical shortcomings make falloposcopy more difficult than the more straightforward transfimbrial technique. With more widespread acceptance, endoscopic evaluation of the fallopian tube may become a standard component of the infertility evaluation.

Endoscopes↗