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At least 1,063 records · Page 59Linked to original sources
PubMed2002

Fluorescein-potentiated argon laser therapy improves symptoms and appearance of corneal neovascularization.

PURPOSE: To evaluate patients' self-assessment of their symptoms and cosmetic appearance after fluorescein-potentiated argon laser treatment (FPAL) for corneal inflammation associated with neovascularization. To describe the objective effects of FPAL therapy on established corneal neovascularization with secondary corneal edema and lipid keratopathy. METHODS: Fifteen patients with corneal neovascularization resulting from various infectious and traumatic etiologies were enrolled in an open, nonrandomized pilot study. Treatment consisted of repeated sessions of intravenous fluorescein, a photosensitizer, combined with blue-green argon laser applications to abnormal corneal vessels. Patients completed a written survey to grade their symptoms and cosmetic appearance prior to treatment and at the end of the follow-up period. Objective changes in signs after FPAL treatment were described by two nonmasked ophthalmologists after slit-lamp examinations and review of serial corneal photographs. RESULTS: Clinical symptoms of blurred vision (p <or= 0.003), photophobia (p <or= 0.03), and cosmetic appearance ( p <or= 0.02) were found to be significantly improved by the patient's subjective assessment survey. There was a trend toward pain reduction (p <or= 0.06) but no apparent reduction in tearing or foreign body sensation (p <or= ns). Objective signs of corneal edema, neovascularization, and lipid keratopathy appeared to be reduced after FPAL therapy. CONCLUSION: FPAL therapy for corneal neovascularization, edema, and lipid keratopathy resulted in a significant reduction in symptoms and improved quality of life for 14 of 15 (93%) patients. A controlled clinical trial to confirm the reported improvement in signs observed in this pilot study should be considered.

Adolescent↗
PubMed1982

Blood pressure drop as a result of fluorescein injection.

Our experience with intravenous fluorescein involved 38 administrations in 29 patients. In all, 24 percent were associated with a drop in blood pressure of 20 mmHg or more and 8 percent with a blood pressure fall of 60 mmHg or more. We believe that physicians using fluorescein in bolus form should be aware of the problem of blood pressure reduction and be prepared to take restorative measures when necessary.

Aged↗
PubMed1983

Wide field fluorescein angiography by use of contact lens.

A system has been developed to obtain fluorescein angiographs with a field measuring to 90 degrees. This involves a contact lens and a new fundus camera with a basic field of 60 degrees. The contact lens, however, can be used with any fundus camera and will result in an increase in the area of the photographs of over 2X. There are numerous clinical applications for high resolution, high contrast, wide field fluorescein angiography.

Contact Lenses↗
PubMed1987

Use of directly acquired digital fundus and fluorescein angiographic images in the diagnosis of retinal disease.

Direct on-line acquisition of digital fundus and fluorescein images is a useful alternative to conventional fundus photography. Immediately after acquisition, digital images may be electronically reassembled, manipulated, and displayed, avoiding the delays inherent in film development. Digital fundus images, conventional photographs, and fluorescein angiograms were obtained at the same sitting in 50 consecutive patients with retinal disease. Digital studies were displayed on a video monitor and diagnoses were made directly from the screen. The interpretations based on digital images alone were virtually identical to those made from photographs, although the resolution of digital images was not equal to that of the photographic images. Despite this disadvantage, the system proved extremely versatile, and for this reason, digital image acquisition was used to routinely document the retinal findings in the majority of the clinical patients.

Fluorescein Angiography↗
PubMed1993

Use of digitized fluorescein angiogram system to evaluate laser treatment for subretinal neovascularization: technique.

In patients with subretinal neovascular membranes (SRNVM), persistence of the SRNVM after laser treatment is a major cause of decreased visual acuity. Persistence is associated with inadequate initial laser treatment. A technique using a digitized fluorescein angiogram (DFA) system is presented that allows immediate evaluation of the adequacy of laser treatment for SRNVM. This involves overlaying a red-free photograph taken after treatment on a fluorescein angiogram taken before treatment using a digital angiogram system that evaluates the adequacy of treatment. A protocol for augmentation of laser treatment has been developed that will aid in the reduction of persistence after laser treatment of SRNVM.

Aged↗
PubMed1994

Macular changes and fluorescein angiographic findings after repair of proliferative vitreoretinopathy.

PURPOSE: To evaluate the status of the macula after surgical treatment of proliferative vitreoretinopathy (PVR). METHOD: Biomicroscopic and fluorescein angiographic examination of the macula was performed in 58 eyes of 58 patients 6 to 7 months after complete gas absorption for rhegmatogenous retinal detachment complicated by PVR (grade C1 to D3). RESULTS: Macular changes were found in 46 eyes (79.3%), and included: cystoid macular edema (30 eyes; 51.7%), epiretinal membranes distant from the macula (3 eyes; 5.1%), macular pucker (7 eyes; 12%), subretinal membranes (5 eyes; 8.6%), and subretinal pigment dispersion (5 eyes; 8.6%). In five eyes, two distinct changes were found. Optic dye leakage occurred in 4 eyes (6.8%). CONCLUSION: Biomicroscopic and fluorescein angiographic examination of the macula can be used to determine why eyes in which rhegmatogenous retinal detachment complicated by PVR is successfully repaired do not achieve good recovery of vision. Spontaneous improvement or disappearance of postoperative cystoid macular edema, with late improvement in visual acuity, may occur in a small number of patients.

Adolescent↗
PubMed1999

Fluorescein and indocyanine green angiographic findings in congenital optic disk pit associated with macular detachment.

PURPOSE: To investigate the pattern of congenital optic disk pits associated with maculopathy using indocyanine green angiography (ICG) and fluorescein angiography (FA). METHODS: Seventeen consecutive patients with unilateral congenital optic disk pit complicated by maculopathy were prospectively enrolled in the study. Complete ophthalmologic examination, color stereophotography, red-free photography, FA, and ICG angiography were performed on all patients during their first examination in our department. RESULTS: Absolute hypofluorescence of the optic disk pit was noted in all eyes on ICG angiography. On the contrary, all eyes showed early hypofluorescence and late staining of the optic pit on FA. All 17 eyes presented a delineated late hyperfluorescence corresponding to the area of macular elevation on both ICG angiography and FA. The intensity of the hyperfluorescence was milder in cases with long-standing maculopathy. CONCLUSION: Imaging of congenital optic disk pits associated or not with macular elevation using ICG angiography has not been reported in the literature. The increased hyperfluorescence in the late phases of the macular elevation in the studied eyes could be attributed to leakage of indocyanine or fluorescein dye into the schisis cavity and the subretinal fluid.

Adolescent↗
PubMed2002

Fluorescein angiographic findings in primary intraocular lymphoma.

PURPOSE: Primary intraocular lymphoma (PIOL), also known as primary central nervous system lymphoma, is a rare yet blinding and fatal disease. Often presenting with ocular involvement, it can masquerade as posterior or intermediate uveitis, thus delaying diagnosis. A noninvasive ancillary test such as fluorescein angiography could be helpful in raising the level of suspicion in the diagnosis of this disease. METHODS: Results of fluorescein angiography (FA) and clinical characteristics of 17 patients (31 eyes) who presented to the National Eye Institute with the diagnosis of PIOL (confirmed by histopathologic analysis) were reviewed. RESULTS: The most common angiographic characteristics included disturbances at the level of the retinal pigment epithelium (RPE), such as granularity (19 eyes [61%]), blockage (17 eyes [55%]), and late staining (14 eyes [45%]). These changes are well correlated to histopathologic findings of lymphoma cells located between the RPE and Bruchs membrane. Perivascular staining or leakage and cystoid macular edema were rare. Other less common findings included pigment epithelial detachments and punctate hyperfluorescent lesions. Clinical characteristics found in eyes for which results of FA were available included vitreitis (29 eyes [94%]), subretinal infiltrates (19 eyes [61%]), and anterior chamber cells (10 eyes [32%]). In some cases, clinical examination did not correlate with FA findings. CONCLUSIONS: Although PIOL may present with a normal angiographic phenotype, extensive RPE changes demonstrated by FA, combined with the absence of perivascular staining or leakage and macular edema, may be associated with and distinctive of PIOL.

Adult↗
PubMed2005

Fluorescein angiographic features of asymptomatic eyes in central serous chorioretinopathy.

PURPOSE: Analysis of the fundus and fluorescein angiography (FA) features of the asymptomatic eyes in patients of central serous chorioretinopathy (CSC) at initial visit and estimation of the overall magnitude of bilateral pathology. METHODS: Retrospective observational case series that reviewed the medical records and FA features of 229 consecutive patients between January 1999 and December 2001. RESULTS: Seventy-three (31.88%) patients had symptoms in one eye but signs in both the eyes, of which 70 were selected. Bilateral symptomatic CSC was seen in 29 (12.66%). Thus, overall bilateral involvement was found in 102 (44.54%) patients. FA features of asymptomatic eyes were pigment epithelial detachment (32.85%), fluorescein leak (31.42%), window defect (31.42%), depigmented patches (14.29%), drusen-like deposits (12.85%), pigment clumps (4.29%), retinal pigment epithelial atrophy (4.28%), subretinal precipitate (2.85%), and sensory retinal detachment (5.71%). Forty-three (61.43%) had evidence of chronic subclinical CSC, whereas 27(38.57%) had features of clinically healed CSC. CSC developed in the asymptomatic eyes within 3 to 12 months in four of the 24 followed-up patients. CONCLUSION: Considerable number of asymptomatic eyes had features suggestive of healed or chronic subclinical CSC. These eyes are at risk for CSC caused by occurrence of active leak(s) from the same or new site(s).

Adult↗
PubMed1981

Hypertensive crisis and myocardial infarction after fluorescein angiography.

Fluorescein angiography is commonly used in evaluation of retinopathy. Adverse reactions are uncommon but severe cardiovascular difficulties seem to occur selectively in older men with hypertension and ischemic heart disease. The 54-year-old patient described had hypertensive crisis and resulting myocardial infarction after fluorescein angiography.

Fluorescein Angiography↗
PubMedDate not supplied

Blue filter discs and nasal endoscopic visualization of fluorescein lacrimal drainage.

For the lacrimal surgeon, fluorescein instilled in the palpebral aperture, and combined with the use of blue filters, can assist office assessment of lacrimal drainage by nasal endoscopy. The fabrication of a separate blue filter attachment for nasal endoscopes, however, is expensive. Inexpensive, heat-stable blue filter discs were sourced from a manufacturer of fiberoptic instruments in Sydney, Australia. These discs can be made in various sizes suitable for standard battery-powered, portable nasal endoscopes. The discs were used in the postoperative assessment of a patient with lacrimal drainage-related pathology. They were easy to use and provided suitable wavelength transmission for improved assessment of fluorescein drainage through the lacrimal system.

Adult↗
PubMed2003

Effect of immunosuppressants on T-cell subsets observed in vivo using carboxy-fluorescein diacetate succinimidyl ester labeling.

BACKGROUND: The in vivo effects of immunosuppressants on T cells are classically determined using animal models of organ transplantation. These methods are technically difficult and time consuming. A simple in vivo method is needed for screening new immunosuppressants. METHODS: Donor mouse spleen cells were labeled with a fluorescent dye, carboxy-fluorescein diacetate succinimidyl ester (CFSE), and then injected into the blood of recipient severe combined immunodeficiency mice. Three days after the injection, spleen cells of the recipient mice were isolated and the proliferating alloreactive T cells were analyzed by flow cytometry. RESULTS: In control recipient mice, 50% of the T cells were proliferating, consisting of both CD4+ and CD8+ T cells. In cyclosporine- or FK506-treated mice, T-cell proliferation was suppressed in the CD4 subset but not in the CD8 subset. On the contrary, T-cell proliferation was significantly reduced in the CD8 subset but not in the CD4 subset in recipient mice treated with rapamycin. CONCLUSION: The present mouse model using carboxy-fluorescein diacetate succinimidyl ester labeling is simple and fast. It is useful for screening new immunosuppressants and for examining the effect on T-cell subsets.

Animals↗
PubMed1978

The use of fluorescein-labelled lectins in the detection and identification of fungi pathogenic for man: a preliminary study.

Fluorescein-labelled lectins of known specificities for different sugars were used in an attempt to identify fungi in paraffin sections of surgical and post-mortem material. Aspergillus fumigatus, Blastomyces dermatitidis, Candida albicans, Cryptococcus neoformans, Paracoccidioides brasiliensis and Rhizopus oryzae have been studied with five fluorescein-labelled lectins and with basis of differences in their reactions with these stains. The results accord well with what is known of the chemistry of the organisms and the method offers promise to practising histopathologists.

Aspergillus fumigatus↗
PubMed1992

Enhancing effects of fluorescein on beta-lactam rash. I: High incidence of cefclidin rashes in an ophthalmological volunteer trial.

Disseminated maculopapular eruptions were frequently observed in a volunteer trial of cefclidin's use in ophthalmological and neurological examinations (8/12; 67%) It appeared at 8-12 days (mean +/- SD, 9.6 +/- 1.1 days) from the initiation of the trial and subsided within 1-2 days (mean +/- SD, 1.8 +/- 0.4 days). Patch testing with cefclidin produced a +/- reaction in 1 of 8 cases, and the drug-induced lymphocyte stimulation test (DLST) elicited a positive response (SI: 2.8) in 1 of 8 and a weakly positive response (1.8 < or = SI < 2) in 2 of 8. From these findings, it seems likely that the eruptions may be partially mediated by delayed type hypersensitivity (DTH) reactions to cefclidin. No such eruption was observed in the phase II trial of cefclidin where only 2.8% of 1.122 volunteers developed the eruption. The volunteers were given both fluorescein and oxybuprocain in their eyes to measure ocular tension on days -1, 0, 1, 3, 5, 7, 9, 11, 13 and weeks 3, 5, 7 after the initiation of cefclidin. Fluorescein and/or oxybuprocain may affect cefclidin to induce these abnormal reactions in the volunteers.

Adult↗
PubMed2006

Semi-automated detection of the foveal avascular zone in fluorescein angiograms in diabetes mellitus.

BACKGROUND: The foveal avascular zone (FAZ) is known to enlarge in diabetic retinopathy. In a preliminary study, the authors applied a region growing algorithm to fluorescein angiograms to detect the FAZ in a semi-automated fashion. METHODS: The FAZ in 44 fluorescein angiograms of 44 eyes of 41 patients with diabetic retinopathy underwent manual outlining, then analysis with the region growing function of the ENVI image analysis software. The same algorithm was applied after median filtering of the images. RESULTS: Correlation coefficient was 0.98 between the first two authors, 0.89 between the first author and semi-automated detection before median filtering and 0.91 after median filtering. Average surface areas however, were smaller with semi-automated detection (1951 pixels before and 2226 pixels after median filtering) than with manual detection (3032 pixels). CONCLUSIONS: Semi-automated detection of the FAZ is possible, but refinements need to be made in angiogram quality and/or image pretreatment to improve results.

Algorithms↗
PubMed1999

Non convulsive status epilepticus following intrathecal fluorescein injection.

We report a case of non convulsive status epilepticus after an intrathecal injection of fluorescein. The clinical presentation was a confusional state--the epileptic origin of which was confirmed by the electroencephalogram. This rare and relatively benign complication should not bring about worry concerning the fluorescein test used for the diagnosis of a dural defect and the identification of the site of a CSF leak.

Electroencephalography↗
PubMed1997

Video fluorescein angiography of the anterior eye segment in severe eye burns.

PURPOSE: Severe eye burns often result in extensive necrosis of the conjunctiva and episcleral tissue. Video fluorescein angiography was performed to reveal the perfusion of the anterior eye segment after severe eye burns. METHODS: A scanning laser ophthalmoscope was used for anterior segment fluorescein angiography in 12 patients (14 eyes) with severe burns grade III-IV and in 7 healthy volunteers. RESULTS: Necrotic tissues occurred as non perfused areas and remained dark throughout the whole angiogram. In general, the borders from healthy to necrotic conjunctival tissue were sharply demarcated. Thus, the extent of scleral and limbal ischemia could be determined exactly. Injured vessels showed hyperfluorescence with late leakage. Damage of the subconjunctival tissue appeared as a deep weak fluorescence in the early angiography and exhibited patchy leakage in the late angiogram. CONCLUSIONS: Anterior segment angiography provides a basis for deciding the extent of surgical debridement of necrotic tissue in the acute phase of the burn. The determination of the extent of limbal and scleral ischemia may give useful information for early plastic-reconstructive procedures.

Adult↗
PubMed1976

The cyclopentolate provocative test in suspected or untreated open-angle glaucoma. IV. Fluorescein angiography of the vessels of the iris in open-angle glaucoma eyes with a positive cyclopentolate response.

Fluorescein angiography of the iris (IFAG) was performed on 15 patients with a positive cyclopentolate response (IOP elevation greater than or equal to 8 mmHg) in 17 eyes to the cyclopentolate provocative test. The chamber angles were open in all the eyes. Seven of the responder eyes had capsular glaucoma undergoing treatment, six had simple glaucoma, two had pigmentary glaucoma and two suspicion of open-angle glaucoma. The object was to study with IFAG whether vascular changes can be established in the iris of the responder eyes such as could have a role in the elevation of IOP. All the eyes with capsular glaucoma displayed vascular changes, vasoproliferation and fluorescein leakage from the iris vessles. No other vascular changes were seen in the irises of the responder eyes. IFAG revealed no differences in the iris vasculature between responder and non-responder eyes. A vascular aetiology for the IOP elevation in responders is improbable.

Cyclopentolate↗