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PubMed1980

[Principles for optimizing and gauging certain processes in vaccine production technology. V. Complex approach to gauging processes involved in assuring aseptic conditions in incubators].

The contamination of the cultivator with the extraneous microflora in the process of growing microorganisms in various apparatus has been shown to have the probabilistic character. The index of aseptic effectiveness is recommended for the comparative evaluation of the effectiveness of the processes which ensure the achievement and maintenance of aseptic conditions in the process of the cultivation of microorganisms in the laboratory and industrial equipment, as well as for changing the scale of the process.

Antisepsis↗
PubMed1981

Efficacy of double-gloving as a barrier to microbial contamination during total joint arthroplasty.

UNLABELLED: After ten total hip replacements in which a double-glove technique was used, 275 outer and inner gloves were tested for microbial contamination and for holes. The operating rooms were equipped with specially designed air-filtration mechanisms. The results indicated that the gloves that most frequently were contaminated were the ones used exclusively for draping. This finding suggests that using a separate pair of outer gloves only for draping is a valuable technique during total hip replacement. CLINICAL RELEVANCE: Although the practice of using double gloves during a total joint-replacement procedure is accepted widely, there is little evidence that double-gloving actually decreases microbial contamination. The results of this study indicate that changing outer gloves at appropriate times during the procedure is indeed an important way to minimize contamination.

Antisepsis↗
PubMed1982

Method for testing aseptic technique of intravenous admixture personnel.

A method of checking the aseptic technique of personnel that prepare i.v. admixtures is described. The following sterile dosage forms of trypticase soy broth (TSB) were prepared: (1) single-strength ampuls 5 ml, (2) six-times (6X) concentrated broth ampuls 10 ml, (3) lyophilized broth sufficient to make 5 ml single-strength broth in 10-ml vials, and lyophilized broth sufficient to make 10.6 ml 6X concentrated broth in 20-ml vials. Admixture personnel were told these were investigational products, and they were given simulated orders to prepare syringes and 50-ml piggyback admixtures of the products. The specimens containing the broth were intercepted by an investigator, incubated for seven days at 37 degree C, and observed for turbidity on days 2 and 7. To verify that the broth supported the growth of bacteria, two common contaminants were inoculated into the broth in the syringes and admixtures to serve as positive controls. At least 100 samples from each of four procedures were processed during the three-month study period. A total of 405 samples prepared by 13 individuals were tested. None of the samples became turbid; hence, the bacterial contamination rate was 0%. Among the positive controls, 98% inoculated with one species and 100% inoculated with the other became turbid. This method is now used for monitoring the aseptic technique of new admixture personnel. This unique method of checking the aseptic technique of personnel is accurate and cost effective, and it avoids the risk of adventitious contamination.

Antisepsis↗
PubMed1982

Maintenance of sterility in urinary drainage bags.

When the outlet tube of urinary drainage sets was kept full of 3 per cent hydrogen peroxide at all times, no evidence of bacterial growth in the urine of the collection bag was noted in at least 92.0 per cent of the patients and 93.3 per cent of the drainage periods in a group of 60 patients and 75 drainage periods. One patient had a positive urinary drainage bag culture and a positive bladder culture as a result of improper disconnection of the catheter, and four patients had bacterial growth from urinary drainage bag samples, two of which were possibly due to a faulty culture technique and one of which may have resulted from contamination of amphoteracin B irrigating solution. Hydrogen peroxide, 3 per cent, appears to approach 100 per cent effectiveness as an antibacterial barrier. Only one patient of 60 or 1.7 per cent had a urinary tract infection develop which was related to the improper handling of the drainage set.

Anti-Infective Agents↗