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An Alcaligenes faecalis isolate from turkeys: pathogenicity in selected avian and mammalian species.

An Alcaligenes faecalis isolate of known pathogenicity for turkeys was examined for adherence and cytotoxicity in tracheal organ cultures of turkeys, chickens, Japanese quail, guinea pigs, hamsters, and mice, and for colonization and pathogenicity in these 6 species. Adherence and colonization were detected by fluorescent antibody staining. Infected and noninfected tracheal rings were examined by phase-contrast microscopy for cytotoxicity (ciliostasis, blebing of the cell membrane, and sloughing of the ciliated epithelium). Alcaligenes faecalis adhered to the tracheal rings of all species examined. Cytotoxicity was apparent in the tracheal rings of turkeys, quail, and chickens. Cytotoxicity was not detected in tracheal rings from the mammalian species. Alcaligenes faecalis colonization of turbinates and tracheas of intact turkeys and quail was detected. Clinical signs of alcaligenes rhinotracheitis were observed and histopathologic characteristics of the disease were detected. Chickens, guinea pigs, hamsters, and mice were refractory to infection with this isolate of A faecalis.

Adhesiveness↗

Congenital glottic webs in children. A retrospective review of 51 patients.

This study is a retrospective review of congenital glottic webs in children. Its purpose is to clarify the controversial aspects of this lesion and to offer a more definitive approach to its treatment. The study includes discussions of embryology and anatomy, a thorough literature review, and a detailed statistical analysis of patient data. Fifty-one pediatric cases are reported, with symptoms and endoscopic findings described in detail. Treatment with dilatations, incisions (knife, scissors, laser), stenting, and external laryngeal surgery with keel insertion are evaluated, and results of therapy, both before and after the development of laser surgery, are reported. Of the 51 patients, 22 required tracheotomy and all who completed therapy were ultimately decannulated. Vocal dysfunction was the most difficult symptom to treat, and perfection of the voice was attained only infrequently. However, the ultimate results of therapy, as noted in this series of patients, should counter the pessimistic viewpoints expressed in the literature.

Abnormalities, Multiple↗

[Dangers in the use of gas-sterilized materials].

After gas sterilisation of synthetic materials, some traces of the gases employed (ethylene oxide; formaldehyde) can remain in the sterilised material, and, in the case of moist materials, in drops of water. These gases are carcinogenic and, in case of contact to tissue, can lead to fibrinous inflammation and consequently to stenotising scars. Two cases of laryngotracheitis are described, in which insufficiently aired tracheal tubes were used. On contact with material containing chloride ions, e.g. PVC or body fluids, ethylene oxid can be converted to highly toxic ethylene chlorohydrine. For this reason, it is prohibited to sterilise synthetics containing chlorine ions with ethylene oxide.

Ethylene Oxide↗

Necrotizing tracheobronchitis: case report.

A 33-week-gestation infant with respiratory distress syndrome is reported. At five days of age, acute life-threatening tracheal obstruction occurred, which was relieved after removal of a plug during bronchoscopy. Histologic examination of the plug revealed partially necrotic tracheal mucosa, compatible with the diagnosis of necrotizing tracheobronchitis. At 31 days of age, obstruction recurred due to the development of a tracheal stricture, which resolved after tracheal reintubation (to maintain patency) and corticosteroid therapy. Tracheal stricture may be a long-term complication of necrotizing tracheobronchitis, when the initial episode does not lead to death from obstruction.

Bronchitis↗

Croup.

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Child↗

Antibiotic aerosolization: the effect on experimentally induced Alcaligenes rhinotracheitis in turkeys.

Oxytetracycline hydrochloride was delivered by aerosol twice daily for 3 days to uninfected turkeys and to turkeys experimentally inoculated with Alcaligenes faecalis. The clinical, microbiological, and histological changes in the upper respiratory tracts were studied. No lesions were observed in the tracheas of uninoculated poults exposed to the aerosol. In experimentally infected poults, clinical signs included ocular and nasal discharges and open-mouthed breathing. Histologic lesions included progressive bacterial colonization of ciliated epithelium, loss of cilia, depletion of mucin from goblet cells, and accumulation of inflammatory cells within the tracheal lumen. Aerosolization of oxytetracycline effected a temporary decrease in bacterial colonization and a delay in clinical signs and histologic lesions in infected treated poults compared with untreated infected poults. Bacterial colonization and histologic lesions in the tracheas of both treated and untreated infected poults were similar by 4 days after the treatment was discontinued. This study indicates additional research with bactericidal antibiotics is needed to further evaluate antibiotic aerosolization as a treatment for alcaligenes rhinotracheitis.

Aerosols↗