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Caution--new skin refrigerants.
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Skin refrigerants.
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Refrigerating the Beckman "airfuge" for improved steroid receptor assays.
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[Sclero-refrigeration of varices].
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[Treatment of deep refrigeration--"accidental hypothermia"].
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[An outbreak of Yersinia enterocolitica infection on the Bjärred peninsula. Indications for risks of refrigerated food].
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Comparison of human islet number and viability obtained during purification on Euro-Collins-Ficoll gradients processed on a refrigerated or nonrefrigerated COBE 2991 cell separator.
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Successful resuscitation from cardiopulmonary arrest following deliberate inhalation of Freon refrigerant gas.
Presented is a case of successful resuscitation of cardiopulmonary arrest following inhalation of a fluorinated hydrocarbon. Fluorinated hydrocarbons have a direct cardiotoxic effect. We found no previous reports describing resuscitation with good neurologic outcome of a patient in cardiopulmonary arrest subsequent to inhalation of a fluorinated hydrocarbon. Early bystander cardiopulmonary resuscitation and ACLS provider intervention help to contribute to improved patient survival. We present a case illustrating the utility of basic life support and early advanced life support, followed by a review of the pertinent literature.
Not quite like buying a refrigerator.
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Effect of refrigeration on Mantoux test result.
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[Studies on muscle-meat quality in swine. 1. Incidence of poor quality as well as influence of slaughtering and refrigeration].
The pork quality of M. longissimus dorsi of 1,010 pigs for slaughter in one pork production unit was determined on 17 slaughter days. Measurements taken for that purpose included pH 45 minutes post mortem, pork brightness 24 hours post mortem, and drip loss 24 hours post mortem. The following results were recorded: 1. Quality gaps in terms of PSE pork were recorded from 21.9 per cent of the pork samples stored under cold store conditions. 2. The percentage of cutlet pieces was reduced considerably (10.9 per cent) by shock cooling (-10 degrees C to -18 degrees C over 1.5 to 2.5 hours). This shows the importance of cooling. 3. The differences recorded between mean values were attributable mainly to nonsystematised environmental conditions, with such variation having been effective not only on the day of slaughter. 4. Close correlations were found to exist between drip loss and pH-value as well as between drip loss and pork brightness, while the relationships were less close between pH-value and brightness.
[Experiences during training of a group for tasting bovine meat at an industrial refrigerated abattoir in Venezuela].
During three months, a group of people (meat consumers and active personnel) of a Venezuelan packing house were asked to assign discretionary scores to a sample of 103 beef longissimus steaks in order to familiarize them with the score sheet and to their future job as panelists. Scores assigned to 37 steaks by seven candidates that assisted regularly to these preliminary sessions (out of 12 attendants), were subjected to Analysis of Variance (ANOVA) to determine variability among judges before training. The whole group (n = 12) potential candidates from both sexes, and ranging between 25 and 45 years, were interviewed and pre selected for formal training. Triangle tests were used as selection tool and allowed for dismissing two of them. Training reinforced acquaintance with test procedures and improved natural abilities, sensitivity and memory for test attributes by using an 8-point descriptive scale to describe samples differing in fiber tenderness, amount of connective tissue, overall tenderness, juiciness, and flavor intensity. After 2 mo. of training (5 h weekly) the first panel performance evaluation (PPE) was conducted over 4 days with 3 sessions per day and 3 samples per session with 9 different samples. Data for each candidate were subjected to one-way ANOVA and F-ratio served as criteria to rank candidates' ability for describing each attribute. Panelist 7 and 8 rated last and were excused to conform an 8-member panel. The panel evaluated 300 samples during a 6-mo period and a second PPE was conducted. The second PPE revealed changes in relative ranking positions. Data from the first 40 (out of 300) samples were subjected to ANOVA for testing score (panelist) variability after training. A significant effect of panelist was detected before (P < .05) and after (P < .001) training, but a much greater variability became apparent in untrained candidates. Mean separation for trained panelist only differed in decimal units within the same points or adjacent points of the scale. Results validated methodologies used for panel training and the panel capability for sensorial evaluation of non-processed beef.
Refrigerated stored specimens are not reliable for delayed INR determination.
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Response to the article 'An organic refrigerant for cryosurgery: fact or fiction?'.
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Beware of refrigerator entrapment.
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Recovery of motile, membrane-intact spermatozoa from canine epididymides stored for 8 days at 4 degrees C.
The purpose of this study was to determine how long canine spermatozoa remain motile and with intact membranes when maintained within epididymides stored at 4 degrees C, and to determine whether such stored spermatozoa are able to bind to canine zonae pellucidae. Testes with attached epididymides, obtained from 32 dogs (26 purebred; six mixed breeds) at orchiectomy, were refrigerated at 4 degrees C, and spermatozoa were collected from caudae epididymides at nine time intervals ranging from 5 to 192 h. The effects on spermatozoa that had been refrigerated within epididymides for various times were determined by assaying sperm motility, integrity of plasma membranes and of acrosomes, and measuring binding of membrane-intact spermatozoa to canine zonae pellucidae. Membrane integrity was assessed using a double fluorescent dye, and acrosome integrity by staining with Pisum sativum agglutinin. For the zona-binding assay at various refrigeration time points, duplicate sets of six oocytes each, isolated from ovaries retrieved at elective ovariohysterectomy, were placed into 100 microl droplets of sperm capacitation medium containing 5 x 10(6) spermatozoa/ml. One minute later, oocytes were rinsed vigorously by pipetting, and then incubated for 1 h at 38.5 degrees C in a humidified atmosphere of 5% CO2 in air; the number of membrane-intact spermatozoa bound to zonae were counted. There was no significant decrease in membrane integrity and acrosome integrity of spermatozoa recovered from epididymides stored at 4 degrees C within the first 48 h of refrigeration. In contrast, sperm motility decreased significantly within the first 5 h of refrigeration (P < 0.05), but then declined more gradually thereafter. Some spermatozoa recovered from epididymides that had been refrigerated for 192 h retained their capability to bind to zonae pellucidae, although the mean number of refrigerated spermatozoa (0.4) bound to zonae was less than that of fresh samples (9.0). Membrane integrity of spermatozoa recovered from epididymides refrigerated for various times was highly correlated (r = 0.88) with sperm motility. Even after storage for 192 h (8 days) at 4 degrees C, motile spermatozoa could be recovered from the epididymides, and such refrigerated spermatozoa were capable of binding to zonae. We interpreted these data to indicate that it might be possible to recover functional spermatozoa from postmortem specimens of domestic and nondomestic canids.
Vaccine storage in pharmacies on the Central Coast of New South Wales.
Pharmacies on the Central Coast of NSW were surveyed to assess the ability of refrigerators used for vaccine storage to maintain the recommended temperature range (2 to 8 degrees C). Refrigerators used for vaccine storage were monitored over a 3-day period using a temperature data logger. Fifty-nine (59) retail pharmacies were identified. The response rate was 90% (53/59) and 52 refrigerators were monitored successfully. Only 10 (19%) of the refrigerators maintained temperatures wholly within the recommended range for the 3-day monitoring period. The remaining refrigerators were considered in three groups--refrigerators keeping temperatures in the range 0.1 to 11.9 degrees C, below 0 degree C and above 8 degrees C for most of the time. There were 15 (29%), 12 (23%) and 15 (29%) refrigerators in these groups respectively keeping temperatures in the recommended range for 91%, 19% and 30% of the time. Of the refrigerators achieving temperatures below 0 degree C, none went below -5 degrees C and on average they kept temperatures less than 0 degree C for 49% of the time. This survey highlights the need for vigilance in vaccine storage for immunisation programs to be successful. Of the vaccines affected by freezing, hepatitis B vaccine was identified as being most at risk.