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Sperm recuperation and cervical insemination in retrograde ejaculation.

Seven infertile patients with retrograde ejaculation, in which spermatozoa could be recuperated from the postejaculation urine, were admitted to a sperm recuperation and cervical insemination program. A noninvasive method for sperm recuperation based on urine alcalinization and serial controls to time masturbation has been used. Insemination has been timed according to BBT charts and cervical mucus characteristics. Pregnancy has been obtained in the seven couples after one to eight treatment cycles.

Cell Separation↗

The value of intrauterine insemination with washed husband's sperm in the treatment of infertility.

Twenty-nine infertile couples were treated by intrauterine insemination (IUI) of washed sperm from a sub-fertile husband (n = 16), in cases of gynaecological (n = 3), combined (n = 4) or idiopathic (n = 6) infertility; 116 treatment cycles resulted in 11 ongoing pregnancies. Between 0.25 and 0.45 ml of capacitation medium, containing at least 500,000 pretreated spermatozoa, were inseminated. Pre-treatment of first split fractions was performed by centrifugation and swimming up of motile spermatozoa. The pregnancy per cycle index (P/C) for IUI was 9.5% for a total of 37.9% of all couples treated achieving pregnancy. These results suggest a substantial benefit compared with a calculated six months' cumulative pregnancy rate of 4.2% independent of treatment, for this infertile population. The value of IUI in selected cases of infertility seems obvious but needs further investigation.

Adult↗

Pregnancies from fallopian replacement of immature eggs with delayed intrauterine insemination.

Gamete intrafallopian transfer requires that a woman should not only have patent tubes but should also have had mature eggs collected for replacement. Eggs must be collected as close to ovulation as possible, to give them a good chance of fertilizing upon replacing them directly into the tubes with the spermatozoa. Preliminary results from three patients who received Fallopian replacement of immature eggs followed by delayed intrauterine insemination indicate that maturation of eggs can occur in vivo in the Fallopian tubes. Intrauterine insemination at a later time when the eggs were judged to be mature has given rise to two pregnancies from the three patients with whom this procedure was adopted.

Adult↗

The study of risk habits in reproductive and perinatal epidemiologic research: the use of a donor inseminated population of women.

In this study we assess whether a population of donor inseminated women (ADI) provides an efficient alternative to an open population of women of reproductive age to study the effects of risk habits (cigarette smoking and alcohol consumption) on reproductive and perinatal health. An ADI population can be prospectively monitored before and after pregnancy; women enter the programme because of their husband's infertility. In the pilot clinic every couple asking for first insemination was requested to fill in a self-administered questionnaire on risk habits and the use of medicine. Medical histories of both the man and woman were taken. In 1986 and 1987, 519 women entered the programme. The response rate was 95%. Distribution of the exposure variables of the women currently smoking (52%) and currently drinking (68%) compared well with a general population survey from the town of Rotterdam. With regard to their risk habits and reproductive health, the population of women married to totally infertile men seems representative of women of reproductive age from an open population.

Adolescent↗

Artificial insemination with frozen sperm: protocol, method of analysis and results for 1188 women.

The experience of a sperm bank during a five year period is reported. Artificial insemination by donor (AID) was carried out on 1188 women using frozen sperm prepared in doses of 0.25 ml. A single insemination was called for during each of the first two cycles. The patients were classified in 4 categories: lost to follow-up, successes, dropouts and open cases. The success rate per cycle was about 10 per cent and was very stable for each of the first 12 cycles. The cumulative success rate was calculated by the life table technique adapted for AID analysis. A theoretical success rate (without dropouts) and an effective success rate (with dropouts) were calculated. These two rates were 68 and 55 per cent respectively after 12 cycles. The median delay to conception was 7 and 9 cycles, respectively, under theoretical and effective conditions. The spontaneous abortion rate (17 per cent) and sex-ratio (106 males for 100 females) resemble those for natural reproduction but the success rat per cycle was markedly lower even after adjusting for age.

Adult↗

Retrograde ejaculation: a systematic approach to non-invasive recovery of spermatozoa from post-ejaculatory urine for artificial insemination.

Retrograde ejaculation is an uncommon but treatable form of male infertility. Successful recovery of live spermatozoa from the post-ejaculatory urine for artificial insemination is dependent on careful regulation of pH and osmolarity of the urine into which ejaculation takes place, and separation of the motile spermatozoa from the debris and cells which are found in these samples. Three pregnancies established by artificial insemination of spermatozoa recovered by noninvasive means from the bladders of men suffering from retrograde ejaculation are described. The techniques for preparing the urine for spermatozoal survival, and for removal of cells and debris by sedimentation or buoyant density centrifugation are discussed.

Adult↗

Artificial insemination with cryopreserved donor semen: a decade of experience.

Ten years' experience of artificial insemination with cryopreserved donor semen for 1023 courses in 783 women resulting in 572 pregnancies is reported. A simple approach with multiple inseminations timed on the basis of cycle length, temperature charts, mucus symptoms and signs was used. The life table pregnancy rate was 61% after 12 cycles of treatment and 75% after 24 cycles. Women had a significantly higher rate of pregnancy in second and subsequent courses of treatment, and the pregnancy rate for treatment beyond 12 cycles was significantly less successful. Women over 35 years of age were significantly less successful.

Counseling↗

Artificial donor insemination frozen versus fresh semen.

The results of artificial insemination by fresh and frozen donor semen (A.I.D.) are reported. Life table conception results were 66.3 +/- 3.8 after 6 months for the fresh semen group. Similar results for 2 consecutive programmes of A.I.D. of frozen semen were 44.0 +/- 4.6 and 64.2 +/- 3.4, respectively. The latter group's cumulative pregnancy rate is high and probably reflects improvement in both sperm preservation and insemination techniques. Using improved freezing techniques, the results of A.I.D. with frozen semen are equal to those with fresh semen.

Actuarial Analysis↗

Artificial insemination with the husband's semen after the husband's death.

Artificial insemination using the husband's semen (AIH) has always seemed more acceptable than the same procedure using donor semen. However, the layman may not even have thought of the legal problems or the moral dilemma if in fact a woman is inseminated using her husband's frozen semen after his death. In the USA there are already sperm banks set up by private individuals, generally for the use of those marriage partners when the husband has had a vasectomy and afterwards a child is desired. If such private sperm banks were set up in Britain complex legal problems would follow, quite apart from the moral issue as to whether it was desirable to bring a child into the world deliberately having deprived him of a father from the start. These are the issues which Mr Cusine thinks should be carefully considered before doctors, lawyers and the women potentially concerned are confronted with a new dilemma.

Death↗

Sexually communicable micro-organisms in human semen samples to be used for artificial insemination by donor.

Two hundred and thirty seven semen samples from 10 institutes for artificial insemination by donor (AID) in Belgium and the Netherlands were tested for the presence of Neisseria gonorrhoeae, Chlamydia trachomatis, Mycoplasma hominis, Ureaplasma urealyticum, herpes simplex virus, and cytomegalovirus. The incidence of these micro-organisms in the semen samples was 0%, 6.3%, 4.6%, 35.9%, 0%, and 0.4% respectively, and 47% of all samples were infected with one or more of the micro-organisms. As the ejaculates from which the samples had been taken had already been, or would be, used for AID, the exclusion of microbiological contamination with sexually communicable micro-organisms before insemination is indicated.

Chlamydia trachomatis↗

Success of donor insemination and male diagnosis.

Of 72 women receiving artificial donor insemination with frozen semen, 49 (68%) conceived. The success rate was significantly better in patients whose husbands were azoospermic (79.5%) than in women whose mates were oligospermic (54.5%). This result may be explained on the assumption that the most fertile women in the latter (male oligospermic) group have disappeared through previous natural conception. It may be concluded that the diagnosis of male infertility is a factor influencing the probability of conception following artificial insemination with donor semen.

Adult↗

Pregnancy rate after homologous insemination.

Homologous insemination (AIH) was performed in 151 patients. The pregnancy rate was 27% in 143 patients treated with the cervical cap method. In cases of large ejaculate volume the split-ejaculate technique was applied to several types of sperm quality, resulting in a pregnancy rate of 43%. Of 36 women with diminished fertility, five (14%) became pregnant. The motility of the spermatozoa was correlated with the success rate, with 30% good progressive motility thought necessary to consider insemination.

Adult↗

Opinion on artificial insemination and surrogate motherhood, 1988.

In 1988, the Minister of Justice of the Netherlands issued an opinion on artificial insemination and surrogate motherhood. The opinion does the following, among other things: 1) rejects the requirement that only licensed health care institutions be allowed to perform the gynecological aspects of in vitro fertilization and insemination and the requirement that final responsibility for in vitro fertilization be on the physician, as opposed to responsibility for only the medical aspects of the treatment; 2) labels surrogate motherhood as an undesirable phenomenon and rejects regulation of it; 3) states that a criminal prohibition will be issued on the professional or commercial brokerage of surrogate motherhood; and 4) advises that a physician should refrain from performing in vitro fertilization if intended for surrogate motherhood purposes.

Developed Countries↗

Legislation on artificial insemination, 1 May 1988.

On 1 May 1988, the governing body of the canton of Glarus, Switzerland, voted that homologous artificial insemination would be the only assisted reproduction procedure authorized to be performed in the canton. On 15 March 1989, the Swiss Bundesgericht declared prohibitions on artificial insemination by donor and homologous in vitro fertilization to be unconstitutional. This decision will be reported in Annual Review of Population Law, Vol. 16, 1989.

Constitution and Bylaws↗

[Artificial insemination from a Catholic viewpoint].

The Roman Catholic Church understands the human propagation as a common act of body and soul, which should not be destroyed either by contraception or artificial insemination. This thesis is based on the natural order and meaning of the unity of man and woman. Within its demands on the state, the church differentiates between artificial insemination of married persons and others.

Catholicism↗

Transmission of genital herpes by donor insemination.

This report describes a donor in a therapeutic donor insemination program who asymptomatically acquired a primary herpes simplex virus type 2 (HSV-2) infection from his long-standing sexual partner. His fresh semen was used to inseminate two HSV-seronegative recipients; in one a primary HSV-2 infection developed, and in one it did not. Direct evidence of transmission from donor to recipient was documented by restriction enzyme analysis of the HSV-2 isolates obtained from the donor's semen and from the recipient's cervix. Because of the possibility of asymptomatic acquisition and transmission of HSV-2, semen donors and their sexual partners should undergo serologic screening for genital herpes using new, type-specific HSV serologic techniques.

Adult↗