Regenerative growth in the urodele forelimb following ultraviolet radiation.
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The morphology of single C3-C4 propriospinal neurones (PNs) including the cell body, dendritic tree, axonal trajectory and the pattern of projection and termination of axonal collaterals in the C3-C4 segments was investigated by intra-somatic or intra-axonal injection of horseradish peroxidase. All the C3-C4 PNs could be antidromically activated from the lateral funicle in C6 and the lateral reticular nucleus but not from Th13. Another criterion was that they received monosynaptic excitation from corticospinal fibres in the contralateral pyramid. Twenty-four C3-C4 PNs were successfully stained. They were located in the lateral part of laminae VI-VIII except for two neurones which were located in lamina V and two in lamina IX. Five to eleven dendrites originated from the cell bodies and extended throughout laminae IV-VIII and even into the white matter in the transverse plane and up to 3 mm rostro-caudally. The axonal trajectory from the cell body was usually curved before reaching the lateral funicle. The bifurcation of the stem axon into a descending and an ascending branch was mostly observed in the white matter close to or at the border between the white and grey matter at the level of the cell body. The ascending and descending axonal branches maintained their location in the same part of the lateral funicle. Sixteen out of 24 stem axons gave off collaterals in the grey matter and/or in the white matter. One to five collaterals were given off from the axons in the grey matter.(ABSTRACT TRUNCATED AT 250 WORDS)
Bilateral metacarpophalangeal radiographs of 100 Thoroughbreds were examined for visualization and location of the major nutrient foramen of the proximal phalanx. The foramina were located on the dorsal or palmar aspects of the cortex or were not visible radiographically. Of the 100 horses, 60 had the same pattern in both forefeet. In the remaining 40 horses, the left and right proximal phalangeal foramina were asymmetric. Of the 200 proximal phalangeal foramina (100 horses), 53 were on the dorsal aspect of the cortex and 101 were on the palmar aspect of the cortex; 46 were not visible radiographically. Significant effect of age or gender could not be determined.
The experiment was carried out on the four extremities of six, sexually mature dogs of a different race. Gradual reconstruction of the anatomic structures was performed as a single act after the section of the elements. To reach stability of the forearm we employed different osteosynthetic techniques and succeeded to reconstruct blood vessels, nerves, ligaments and the skin, afterwards. Numerous techniques of suturing material were used. The animals were sacrificed six months after being operated and were exposed to microscopic observation and histological analysis. The results obtained suggested the necessity of a firm osteosynthesis of the forearm bones, use of less traumatic suturing material for suturing both blood vessels and fasciculi when preparating nerves.
Revascularization of the forearm was performed on six mature dogs of a different race. Arteria mediana was cut 2 cm above the fork of the arteria radialis and later sutured with a nonresorptive monofilament thread/Ethilonr/7-0 and 8-0 in diameter. The same process was repeated on the cefalica vein. Arteriographies were performed on the operated extremities, pre-operatively, immediately after operation and while an animal being sacrificed. The subjects were sacrificed six months after the operation. Then microscopic and histological analyses were carried out to assess the quality of the blood vessels at the suturing place. At the place of the blood vessels reconstruction, lumen did not get narrower which indicated that all the vessels were in a perfect functional condition. In a number of dogs a higher collagenic proliferation was noticed, in the vicinity of the blood vessels sutured. In the regenerative process of the arterial wall tunica mediana almost took a predominant position, like in the intact arteria. All the three strata were present with no relevant deviations in structure. In the cefalica vein the regeneration of the connective tissue support of the smooth muscles cells was insufficient. Based on the analysis of the results obtained it might be concluded that after revascularization of the forearm of the dog the regeneration of the blood vessels made the segment functionally capable with the structure not different from the normal one which existed in the blood vessels of the same part of the body.