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Phytoremediation of landfill leachate.

Leachate emissions from landfill sites are of concern, primarily due to their toxic impact when released unchecked into the environment, and the potential for landfill sites to generate leachate for many hundreds of years following closure. Consequently, economically and environmentally sustainable disposal options are a priority in waste management. One potential option is the use of soil-plant based remediation schemes. In many cases, using either trees (including short rotation coppice) or grassland, phytoremediation of leachate has been successful. However, there are a significant number of examples where phytoremediation has failed. Typically, this failure can be ascribed to excessive leachate application and poor management due to a fundamental lack of understanding of the plant-soil system. On balance, with careful management, phytoremediation can be viewed as a sustainable, cost effective and environmentally sound option which is capable of treating 250m(3)ha(-1)yr(-1). However, these schemes have a requirement for large land areas and must be capable of responding to changes in leachate quality and quantity, problems of scheme establishment and maintenance, continual environmental monitoring and seasonal patterns of plant growth. Although the fundamental underpinning science is well understood, further work is required to create long-term predictive remediation models, full environmental impact assessments, a complete life-cycle analysis and economic analyses for a wide range of landfill scenarios.

Biodegradation, Environmental↗

Linking childhood cancer with potential environmental exposure determinants.

Childhood chronic diseases, especially cancer, are of growing concern. Research has focused on 2 developmental periods, prenatal and postnatal. While it is hypothesized that chemical contaminants in the physical environment may play a role in the development and exacerbation of many chronic diseases, the role of environmental exposures in the etiology of these conditions remains uncertain. This can be somewhat attributed to the fact that it is very difficult to efficiently link chronic health effects with environmental exposures that are likely to have occurred temporally and spatially distant from diagnosis. This study explored the utility of linking childhood cancer cases with their birth certificate data as a method for increasing the number of geo-referenced data points available for linking health effect data with environmental monitoring data. This would begin to quantify the transiency of 1 subset of the population, and provide a basis for characterizing and estimating potentials for exposure to numerous environmental contaminants during prenatal and postnatal periods. A total of 441 unduplicated cancer diagnosis records of children who were both born and diagnosed with cancer in Wisconsin between 1995 and 2002 were linked with birth records to explore the variability between address at birth and diagnosis. The majority of records were matched to a birth record file (81.0%). Of these matched records, 86% moved <1 mile from birth to time of diagnosis. The results suggest that administrative and public health surveillance data can be used to quantify transiency. Data from the sample tested indicate that during the given time period children do not move far from their birth homes, suggesting minimal changes in exposure potential related to residence location from birth to diagnosis. This background is useful for future epidemiological investigations linking environmental factors with chronic health effects.

Child↗

Biosensing paraoxon in simulated environmental samples by immobilized organophosphorus hydrolase in functionalized mesoporous silica.

There is a critical need for highly sensitive, cost-effective sensors to conduct ecological analyses for environmental and homeland security-related applications. Enzyme biosensors, which are currently gaining acceptance for environmental monitoring applications, need improvements to deliver faster measurements with stabilized sensing elements, e.g., enzymes. We report here on a method which significantly overcomes this difficulty, and demonstrate its application in a biosensor for aquatic environmental applications. A fast-responding and stable biosensor was developed via immobilization of organophosphorus hydrolase (OPH) in functionalized mesoporous silica (FMS) with pore sizes in tens of nanometers. The OPH-FMS composite was held on glassy carbon electrode by a dried Nafion gel and FMS protected OPH from Nafion-resulted activity loss. The resulting enzyme biosensor, when integrated with an electrochemical instrument, responded rapidly to low paraoxon concentration and achieved steady-state current in less than 10 s, with a detection limit of 4.0x10(-7) M paraoxon. The biosensor was tested for detection of paraoxon in simulated environmental samples, under wide-ranging physicochemical conditions. Results clearly indicate high recovery efficiencies in aqueous solutions (96 to 101%) at different pH, total organic carbon, total dissolved solids, and total suspended solids, and demonstrate the ability of the biosensor unit to continuously monitor paraoxon in aqueous conditions similar to those found in river and lake systems.

Aryldialkylphosphatase↗

A survey of blood lead levels in dogs and cats.

The blood of 206 dogs and 26 cats was analyzed for lead by non-flame atomic absorption giving mean levels of 6.4 mug/100 ml for dogs and 5.2 mug/100 ml for cats. Statistical treatment of the results using a paired Students' t-test showed significant differences by age for dogs but not for cats. Urban dogs had significantly higher blood lead levels than country dogs (P less than 0.05) thus indicating that dogs can be regarded as useful environmental monitors for lead pollution.

Age Factors↗

Decision support methodologies for human health assessment of toxic substances: Agency for Toxic Substances and Disease Registry's perspectives on collaboration and infrastructure development among government, academia, and industry.

ATSDR's mission is to prevent or mitigate adverse human effects and diminished quality of life that result from exposure to hazardous substances from hazardous waste sites, unplanned releases, and other sources of pollution present in the environment. As part of this charge, human health assessments are performed through the use of data from animal studies environmental monitoring data and human epidemiological studies. Often sufficient data are not available to perform such assessments. Hence, modeling approaches are often used to estimate the likelihood of exposure to environmental chemicals and adverse health effects to human populations. In the last two decades, several computational techniques have been recognized as having potential to improve the accuracy of this process. As part of the assessment process, (1) chemicals of potential concern must be identified, (2) available data on their toxicity evaluated, (3) quantitative measures of their potential adverse health effects developed, and (4) exposure assessments made. The human health risk must then be characterized in a useful and understandable manner.

Animals↗

Medical surveillance and screening in the workplace: complementary preventive strategies.

The terms medical surveillance and medical screening have sometimes been used interchangeably in the occupational medicine literature. Recently attempts have been made to redefine these as mutually exclusive, by nesting surveillance under screening or vice versa, by subsuming both under medical monitoring or periodic examinations, or by eliminating use of the term medical surveillance altogether. In this paper I argue that medical surveillance and medical screening represent discrete concepts and approaches and should be viewed as distinct and complementary secondary preventive components of an occupational health program. Medical surveillance refers to the periodic examination of putatively exposed workers, and is therefore a longitudinal approach. Medical screening refers to the cross-sectional testing or evaluation of a group of workers. Biological monitoring, whether defined narrowly or broadly, focuses on exposure to particular toxicants and should serve as a component of medical surveillance (or of medical screening), not as an independent complement to environmental monitoring. Medical surveillance must function in the context of a comprehensive occupational health program that includes industrial hygiene and education and does not in itself suffice as a comprehensive occupational health program. It is useful to clarify these terms because some of the ethical issues as well as medical controversies over their utility arise from a misunderstanding of what constitutes medical surveillance and how it should be applied.

Cross-Sectional Studies↗

Temporal stability of blood lead concentrations in adults exposed only to environmental lead.

The temporal stability of blood lead concentrations of 21 healthy adults (14 men and 7 women) exposed only to environmental lead was assessed by analysis of 253 blood specimens collected serially over periods from 7 to 11 months. Improved analytical sensitivity allowed detection of small (less than 1.0 microgram/100 ml) changes in blood lead concentrations and both within- and between-run analytical errors were minimized by a strict internal quality control protocol. The women had lower blood lead concentrations (mean 8.5, range 7.4-10.8 micrograms/100 ml) than did the men (mean 12.2, range 8.6-15.8 micrograms/100 ml). These are within the expected ranges for non-occupationally exposed persons. Blood lead concentrations in the serial specimens from both men and women changed very little over the study period, with standard deviations of less than 0.5 micrograms/100 ml for the majority of individual mean concentrations: for all except two subjects the standard deviations were less than 0.8 micrograms/100 ml. Two subjects showed significant changes in blood lead concentrations during the study (standard deviations of mean greater than 1.0 micrograms/100 ml). A temporary increase in oral lead intake was identified for one of these subjects. In the absence of substantial changes in lead exposure blood lead levels in adults are remarkably stable and for their environmental monitoring a single blood lead concentration is an excellent biological indicator.

Adult↗

Evaluation of absorbed radiation dose to working staff during cardiac catheterization procedures.

BACKGROUND: Cardiac catheterization has been used frequently for the evaluation and treatment of patients with heart diseases. The working staff, particularly cardiologists who perform these procedures, have the highest potential risk of receiving high radiation doses due to close contact with patients. The purpose of this study was to measure and evaluate the accumulated radiation dosage of the cardiologists while they were performing clinical procedures in the cardiac catheterization laboratory. The working environment of the catheterization laboratory was also monitored for radiation. METHODS: Thermoluminescent dosimeters (TLDs) with very high sensitivity were employed for dose evaluations. They were taped to various parts of the body of the cardiologists during catheterization procedures. For environmental monitoring, TLDs were also distributed at several sites of the catheterization rooms for a period of 2 to 4 weeks. RESULTS: The study showed that the left wrist of the cardiologists received the highest radiation dose (338 microsieverts [microSv]/procedure) and the left lens received the second highest dose (149 microSv/procedure) during the procedures. The dose to the knees was unexpectedly high (92 microSv/procedure), partly due to radiation leakage from the lead curtain shielding under the patient couch. On average, the effective radiation dose per year was 37 mSv/y for a cardiologist who performs 10 catheterization procedures per week. Compare this to the occupational exposure limit of 50 mSv/y. The estimated accumulated equivalent dose to the lens was 152 mSv/y, which exceeded the regulatory limit for occupational exposure. CONCLUSIONS: Using proper lead shielding and increasing the distance from the radiation source are good strategies for reducing the radiation dose in medical staff. The work area outside the catheterization room was considered safe because the radiation level was essentially equivalent to the background radiation level.

Cardiac Catheterization↗

Exertional heat illness and environmental conditions during a single football season in the southeast.

CONTEXT: Recommendations for heat illness prevention provided by sports medicine associations do not always account for sex differences, specific age populations, regional environmental conditions, equipment worn during activity, or the athlete's size or preexisting level of fitness. OBJECTIVE: To evaluate the rate of exertional heat illness (EHI) among collegiate football athletes and to monitor environmental conditions during American football practice for a 3-month period. DESIGN: Epidemiologic study in which we reviewed the occurrence rates of EHI and wet bulb globe temperature readings during a 3-month period of American collegiate football practice sessions. SETTING: Five universities in the southeastern region of the United States. PATIENTS OR OTHER PARTICIPANTS: Collegiate football players at the 5 universities. MAIN OUTCOME MEASURE(S): Wet bulb globe temperatures were recorded from August through October 2003, at the beginning, middle, and end of each practice session. The EHIs were identified and recorded, and athlete-exposures (AEs) were calculated. RESULTS: A total of 139 EHIs and 33 196 AEs were reported (EHI rate = 4.19/1000 AEs). The highest incidence of EHIs was in August (88%, EHI rate = 8.95/1000 AEs) and consisted of 70% heat cramps (6.13/1000 AEs), 23% heat exhaustion (2.06/ 1000 AEs), and 7% heat syncope (0.58/1000 AEs). No cases of heat stroke or hyponatremia were identified. The highest risk of EHI occurred during the first 3 weeks of the study; mean wet bulb globe temperature declined significantly as the study continued ( P < .001). Temperatures in the final 5 weeks of the study were significantly cooler than in the first 5 weeks ( P < .05). CONCLUSIONS: Heat cramps were the most common EHI and occurred most often during the first 3 weeks of practice. Athletic trainers should take all necessary preventive measures to reduce the risk of EHI.

Journal Article↗

An assessment of the radiation dose received by staff using fluoroscopic equipment.

Measurements of scattered radiation were made on two fluoroscopic installations, one with an overcouch tube and one with an undercouch tube. The annual doses to staff involved in a fluoroscopic procedure undertaken on both types of unit were estimated using measured dose rates and weighting factors referred to in ICRP 26. Environmental monitoring at the protective barrier was performed for various room layouts to determine the effect of both equipment and room design on the annual dose received by staff at the control panel. In all circumstances the annual dose to staff inside the control cubicle was negligible.

Environmental Exposure↗

[Environmental and biologic monitoring of atrazine exposure at a formulating plant].

Atrazine exposure was evaluated in three workers engaged in technical Atrazine dust formulating and bagging processes, by environmental and biological monitoring. We found an Atrazine concentration in breathing area of 31.2 micrograms/m3 (SD +/- 9.9); actual inhalatory absorption was 20.7 micrograms/workshift. Cutaneous amount was 6 mg per workshift and the largest fraction was found on hands. Supposing that only 3.5% of the applied was absorbed, 239.7 micrograms of Atrazine were taken up by skin per workshift. The dealkylated metabolites and parent compound excreted in urine, as a whole, accounted for 71.09% of the presumed total dose absorbed. The spectrum or the urinary Atrazine metabolites comprised bi-dealkylated (58%), deethylated (30.7%), deisopropylated (7.7%) and unmodified Atrazine (3.6%). About 31% of the amount is excreted during the workshift itself. These findings allow to evaluate the magnitude of the real doses absorbed, keeping into account the effect of protective means.

Atrazine↗

Biosensors and enzyme immobilized electrodes.

A biosensor is a device which consists of a biological sensing element connected to a transducer. The transducer can be electronic, optical, electrical, etc. This emerging technology offers us a powerful tool which is radically altering our approach to analytical methods. It was realised that enzymes are natural sensors on account of their highly selective nature. Much of the impetus to the work has come from medical requirements. Instant analysis of clinical samples has an obvious appeal to physicians and patients alike. Of particular interest is the possibility of continuous 'in-vivo' monitoring of metabolites, drugs and proteins using miniature, portable systems. In recent years, there has been a growing demand for biosensors in the fields of veterinary science, animal husbandry, the food industry and environmental monitoring. However, the possibility of successful application rests upon future developments. Increasing attention will have to be paid to the engineering of both the basic components and the device on the whole. New biochemical reactions will either have to be discovered or engineered through genetic manipulation or chemical techniques. Optimization of response time, selectivity, stability and low costs should receive priority considerations.

Biosensing Techniques↗

Genotypic diversity of Vibrio isolates associated with turbot (Scophthalmus maximus) culture.

One hundred environmental strains of Vibrio isolated from seawater and skin of healthy turbot in an aquaculture system were analyzed. Chromosomal DNA was digested with HindIII and MluI, and hybridized using a digoxigenin-labeled probe complementary to 16S and 23S rRNA of Escherichia coli. Nineteen riboclusters were defined by ribotyping analysis at a value of SD> or = 70%, using the Dice coefficient (S(D)) and UPGMA. The phylogenetic position of each ribocluster was achieved by 16S rRNA gene sequencing of representative isolates. Both techniques were necessary and useful for identifying the isolates. V. parahaemolyticus, V. scophthalmi, V. splendidus-V. lentus related group, V. halioticoli, V. fischeri and V. ichthyoenteri were identified and clustered separately. Their ribocluster diversity was studied. Throughout the year, ribotypic profiles of corresponding strains isolated from both seawater and turbot skin appeared, indicating that environmental strains can easily colonize turbot. No correspondence between riboclusters and the season of isolation was found. Some ribotypes had been found in previous studies, demonstrating that ribotyping is a useful tool for monitoring environmental isolates and to finding strains that can colonize aquatic organisms and are able to produce outbreaks. The ribotype schemes defined here can be used as a ribotype database of environmental isolates of these species.

Animals↗

Heavy metals: sources and environmental presence.

The Istituto Superiore di Sanità has for many years been involved in the problem of environmental pollution caused by heavy metals. A brief review of the sources of this pollution is first presented which also includes the presence in nature of some heavy metals which are among the most dangerous. Data gathered by the authors is then presented from sampling of the emissions of urban solid waste (USW) incinerators and from environmental monitoring programs in different areas in Italy according to four categories: urban, industrial, rural and mixed.

Environmental Pollutants↗

The properties of geophysical fields and their effects on elephants and other animals.

Geophysical properties of acoustic, seismic, electric, and magnetic waveforms create opportunities and constraints for animals' communication and sensory monitoring of the environment. The geometric spreading of waves differs; at some frequencies, transmission is most efficient and has minimal noise. The spreading properties of seismic waves favor long-distance propagation for communication and environmental monitoring, and would benefit elephants (Elephas maximus and Loxodonta africana), such as in locating subsurface water. Extending C. E. O'Connell-Rodwell, B. T. Amason, and L. A. Hart (2000), a man jumping at 1.11 km propagated seismic waves at 10-40 Hz. Given the noise of lightning and the Schumann resonances, near field magnetic and electric transmission by animals would be most efficient around 1000 Hz.

Animal Communication↗

A regression model using sediment chemistry for the evaluation of marine environmental impacts associated with salmon aquaculture cage wastes.

This study was undertaken to develop an approach for modelling changes of sediment chemistry related to the accumulation of aquaculture waste. Metal composition of sediment Al, Cu, Fe, Li, Mn, and Zn; organic carbon and < 63 microm particles were used to determine the extent of detectable effects around the cage. This study showed marked differences in the sediment chemistry between aquaculture sites and the natural background: (1) negative correlations between sediment Cu and Zn with Al, (2) poor correlations between metals and Li, and (3) concentrations of Fe and Mn decreased with increased accumulation of organic carbon. There is a trend among normalised metals, organic carbon and particles related to normal, hypoxic and anoxic sediment conditions. The trends are useful for detecting and assessing the cumulative effects from aquaculture wastes to the marine environment. Lithium is less interactive with other metals in aquaculture sediments compared with the natural background sediments. Principal components analysis (PCA) was carried out on the metals, organic carbon, and particles to cluster the similarities of the variables so as to establish the predicted or adjusted environmental monitoring program (EMP) ratings. This approach, using the adjusted EMP rating based on sediment chemistry, yields a regression model with R2 = 0.945 compared to R2= 0.653 for the regression model using unadjusted EMP for assessing the environmental conditions.

Animals↗

Predictive exposure modelling--a case study with a detergent surfactant.

Environmental exposure estimations are generally based on a knowledge of how and in what quantity a substance enters the environment and how it may subsequently be distributed and transformed. Once present within the environment, biota (including man) may be exposed. This paper outlines the tools commonly used to estimate environmental exposure to ingredients from detergents and other household products. Such products are typically manufactured in large quantities, used by many people, and disposed of after household use into the environment via the sewer. The vast majority of this waste stream is treated via domestic wastewater treatment plants (WWTPs) as documented in the sewage treatment Directive 91/275/EEC. WWTPs significantly reduce the load of chemical substances to the receiving surface waters, and have become an intrinsic part of exposure and risk assessment of household chemicals. WWTP models are generally first-order (e.g. SIMPLETREAT, WWTREAT) or mixed-order (e.g. Monod) kinetics and can exhibit, potentially, very distinct dependencies on the influent concentration. Thus, the correct representation of xenobiotic behaviour in a WWTP and modeling of their fate has a significant impact on exposure assessment. The Environmental Risk Assessment Steering Committee (ERASM) of the Association Internationale de la Savonnerie et la Détergence, et des Produits d'Entretiens (AISE) and the Comité Européen de Agents de Surface et Intermédiares Organiques (CESIO) has commissioned a joint industry Task Force of the Association to develop and apply specific methodology for the environmental monitoring of surfactants, and verification of fate models. The monitoring programmes have been designed to (1) establish the fate, distribution and concentrations of the major surfactants used in detergents-linear alkylbenzene sulfonate (LAS), alcohol ethoxylates (AE), alcohol ethoxylated sulfates (AES) and soap in relevant environmental compartments and (2) to provide the necessary data for checking the applicability of mathematical models to predict their fate and concentrations in these environmental compartments. The case study detailed here, specifically focuses on the refinement of the LAS exposure assessment for surface waters in The Netherlands.

Animals↗

Geographical differences in cancer incidence in the Amazon basin of Ecuador in relation to residence near oil fields.

BACKGROUND: Since 1972, oil companies have extracted more than 2 billion barrels of crude oil from the Ecuadorian Amazon, releasing billions of gallons of untreated wastes and oil directly into the environment. This study aimed to determine if there was any difference in overall and specific cancer incidence rates between populations living in proximity to oil fields and those who live in areas free from oil exploitation. METHODS: Cancer cases from the provinces of Sucumbios, Orellana, Napo and Pastaza during the period 1985-1998 were included in the study. The exposed population was defined as those living in a county (n = 4) where oil exploitation had been ongoing for a minimum of 20 years up to the date of the study. Non-exposed counties were identified as those (n = 11) without oil development activities. Relative risks (RR) along with 95% CI were calculated for men and women as ratios of the age-adjusted incidence rates in the exposed versus non-exposed group. RESULTS: The RR of all cancer sites combined was significantly elevated in both men and women in exposed counties. Significantly elevated RR were observed for cancers of the stomach, rectum, skin melanoma, soft tissue and kidney in men and for cancers of the cervix and lymph nodes in women. An increase in haematopoietic cancers was also observed in the population under 10 years in the exposed counties in both males and females. CONCLUSION: Study results are compatible with a relationship between cancer incidence and living in proximity to oil fields. An environmental monitoring and cancer surveillance system in the area is recommended.

Adolescent↗