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PubMed2004

Integrated pest management approach for a new pest, Lacanobia subjuncta (Lepidoptera: Noctuidae), in Washington apple orchards.

Bioassays of Lacanobia subjuncta (Grote and Robinson) larvae established baseline LC50 values and identified the potential of reduced-risk, organophosphate replacement and naturally derived insecticides (eg chloronicotinyls, spinosyns, oxadiazines, insect growth regulators, microbial insecticides and particle films) to control this pest. The toxicities of these products were compared with those of organophosphate, carbamate, chlorinated cyclodiene and synthetic pyrethroid insecticides used in the management of lepidopteran pests in Washington apple orchards. Field trials were conducted comparing candidate insecticides to conventional alternatives. Several new insecticides (eg spinosad, methoxyfenozide, indoxacarb and an aluminosilicate particle film) proved to be effective for the management of L subjuncta. We summarize the goals and challenges of developing an integrated pest management program for new and resurgent pests as insecticide tools continue to change, and propose a hypothesis for the sudden increase in pest status of L subjuncta based on organophosphate tolerances. The role of novel insecticides with unique modes of action in resistance management and the encouragement of biological control are also discussed.

Animals↗
PubMed2004

Chemical residues and bioactivity of tebufenozide applied to apple foliage.

Tebufenozide, an insect growth regulator that acts as an ecdysone agonist, was applied at recommended label rate to apple trees in August 1997 and May 1998. Foliar residues were determined by high-performance liquid chromatography from samples collected 2, 24, 48 and 96 h post-spray, and at weekly intervals thereafter for 9 weeks in 1997 and 11 weeks in 1998. Foliage sampled at the same times was used in bioassays to determine residual toxicity to first-instar obliquebanded leafrollers, Choristoneura rosaceana (Harris). Residue decay followed first-order kinetics in both years, with residual half-life of 36.3 days in 1997 and 7.2 days in 1998. Estimates of the time needed to reduce bioactivity to 50% in bioassays were 18.7 days in 1997 and 36.3 days in 1998. The accuracy of equations describing decay of tebufenozide residues and bioactivity over time was not improved by using degree-day accumulations as the independent variable.

Animals↗
PubMed2004

Detection of phenolic oxidation products in cider apple juice by high-performance liquid chromatography electrospray ionisation ion trap mass spectrometry.

Juice was prepared from cider apples of the cultivar "Kermerrien" under oxidative conditions. After isolation by solid-phase extraction, the phenolic fraction was subjected to high-performance liquid chromatography/electrospray ionisation mass spectrometry. SIM scans were performed at m/z values obtained in model solutions. The oxidation products, resulting from coupling between a molecule of caffeoylquinic acid and caffeoylquinic acid, catechin or dimeric flavan-3-ol, were detected.

Beverages↗
PubMed1998

Particle development in apple juice determined by light scattering and electron microscopy.

Light scattering was used to detect the kinetic development of particles in apple juices produced with and without oxidation and in procyanidin extracts prepared from these two juices. Particle size developed exponentially in both oxidized and unoxidized juices, suggesting an enzymatic origin for the particle forming reactions. The procyanidin extract from oxidized juices produced particles which grew linearly, suggesting diffusion-controlled aggregation or coalescence of particles. Procyanidins from unoxidized juice showed no particle development over at least 60 days of storage. Electron microscopy showed particles similar to those seen previously and some newly described morphologies. The mechanism of haze particle development is discussed in the light of the light scattering and electron microscopic results.

Beverages↗
PubMed2004

Toxicity of pesticides to predatory mites and insects in apple-tree site under field conditions.

Various applications of active ingredients of six fungicides and three insecticides and acaricides at normally recommended dosages were tested on two predatory mite species (Amblyseius andersoni Chant and Anthoseius bakeri Garman) from the family Phytoseiidae and on two predatory insect species (Coccinella septempunctata L. Chrysopa perla L.) dominantly present on apple trees. Small differences were found between fungicide treatments. On the trees treated with six fungicide applications the predatory mites and insects survived and increased to a high level, often 20-40 phytoseiids per 100 leaves and 4-8 predatory insects per sample unit. Only the active ingredients tolylfluanid and myclobutanil resulted in lower densities of predatory mites (10-20 phytoseiids per 100 leaves). One application of insecticides-acaricides (active ingredients: clofentezine, phosalone) showed no toxic effect on predatory mites and insects. Two applications of phosalone and one of alpha-cypermethrine were slightly or moderately toxic. Two applications of alpha-cypermethrine and eight routine sprays of various insecticides-acaricides and fungicides were very toxic and resulted in the lowest maximum number of predatory mites and insects, approximately 0-10 phytoseiids per 100 leaves and 1-4 predatory insects per sample unit. The toxicity of pesticides to predatory mites and insects is based on the toxicity of the pesticide' active ingredient and the spray frequency. The active ingredients of fungicides and only one or two applications of insecticides and acaricides were not or slightly toxic and could be used in integrated pest management.

Animals↗
PubMed1995

A continuous spectrophotometric method for determining the monophenolase and diphenolase activities of apple polyphenol oxidase.

A continuous spectrophotometric method for the determination of the monophenolase and diphenolase activities of apple polyphenol oxidase is described. The method is based on the coupling reaction between 3-methyl-2-benzothiazolinone hydrazone (MBTH) and the quinone product of the oxidation of p-hydroxyphenyl propionic acid and 3,4-dihydroxyphenyl propionic acid in the presence of polyphenol oxidase. The lambda(max) and molar absorptivity (epsilon) for the MBTH-quinone adduct have been calculated. The presence of MBTH in the reaction medium decreases the lag period during the expression of monophenolase activity. The high value of V(mas) suggests the existence of a high catalytic constant. This, together with the value of epsilon for the MBTH-quinone adduct, makes this method more sensitive than other continuous methods.

Catechol Oxidase↗
PubMed1996

Characterization of the ferrous ion binding sites of apple 1-aminocyclopropane-1-carboxylate oxidase by site-directed mutagenesis.

The putative ferrous ion binding sites (H177, D179, and H234) of apple fruit 1-aminocyclopropane-1-carboxylate oxidase determined by homology comparisons with enzymes which required non-haem Fe2+ for activity were altered by site-directed mutagenesis. The activities of mutants (H177F, D179H, D179A, H234F, and H234D) were completely lost. SDS-PAGE and western immunoanalysis confirmed that loss of enzyme activity in mutants was not due to impaired enzyme expression. These results strongly suggest that H177, D179, and H234 are the Fe2+ binding site.

Amino Acid Oxidoreductases↗
PubMed2001

Effect of cryopreservation on apple genetic resources at morphological, chromosomal, and molecular levels.

Shoot tips of three apple genotypes, namely, Malus pumila cv. M26, Gala, and Hokkaido No. 9, were successfully cryopreserved using a modified encapsulation-dehydration method. As a result, in addition to a high survival rate and regeneration rate, the capacity of shoots regenerated from cryopreserved samples to root was enhanced. Eight M26 single-bud sibling lines were used to assess genetic stability. Although cytological examination revealed a ploidy difference in the noncryopreserved control, the ploidy constitution remained relatively stable during the period of cryopreservation. Amplified fragment length polymorphism (AFLP) assay was performed to detect DNA-level variation. No change in DNA fragment pattern and number was observed between the control and the cryopreserved samples. In addition, methylation-sensitive amplified polymorphism (MSAP) assay was carried out to investigate the DNA methylation status during the period of cryopreservation. It was found that cryopreservation induced a decrease in DNA methylation level.

Base Sequence↗
PubMed1994

Crystallization and preliminary X-ray analysis of recombinant 1-aminocyclopropane-1-carboxylate synthase from apple. A key enzyme in the biosynthesis of the plant hormone ethylene.

Crystals of recombinant 1-aminocyclopropane-1-carboxylate synthase from apple have been obtained with polyethylene glycol as precipitant using a combination of vapour diffusion and macroseeding techniques. The crystals are of space group P2(1), with unit-cell constants a = 53.7 A, b = 69.3 A, c = 123.7 A and beta = 89.9 degrees. The asymmetric unit content is a 1-aminocyclopropane-1-carboxylate synthase dimer with a molecular mass of 94 kDa. Diffraction extends to 2.2 A resolution.

Crystallization↗
PubMedDate not supplied

Cost-effectiveness of smoking cessation modalities: comparing apples with oranges?

BACKGROUND: Although the results of self-help and group treatments for smoking cessation are known, the cost effectiveness and participants' characteristics of these treatments remain mostly undetermined. METHODS: Consecutive samples of 84 self-help manual requesters and 83 group participants in a Dutch community-based smoking cessation program were subjected to telephone interviews before treatment and after a 6-month follow-up. Participants' baseline characteristics were compared and contrasted with a random sample of nonparticipating smokers (N = 924). Cost effectiveness rates were computed from the perspectives of the society, the steering group, and the participants. RESULTS: Participants appeared to be more "hardcore smokers" than nonparticipants. Self-help manual requesters seemed easier quitters than group participants. Self-help was at least three times as cost effective as group treatment from the perspective of the program provider and over four times as cost effective from the viewpoint of the participants. When taking savings through not smoking into account, return on the social investment was positive. CONCLUSIONS: From a social perspective, the project seemed cost effective, compared with medical interventions. Since both modalities attracted different groups of smokers, cost effectiveness comparisons may incorporate the comparison of apples with oranges, and referral to either modality should be based on smokers' characteristics and the societal value placed on health as well.

Adult↗
PubMed1994

Immunocytolocalization of 1-aminocyclopropane-1-carboxylic acid oxidase in tomato and apple fruit.

The subcellular localization of 1-aminocyclopropane-1-carboxylic acid oxidase (ACC oxidase), an enzyme involved in the biosynthesis of ethylene, has been studied in ripening fruits of tomato (Lycopersicum esculentum Mill.). Two types of antibody have been raised against (i) a synthetic peptide derived from the reconstructed pTOM13 clone (pRC13), a tomato cDNA encoding ACC oxidase, and considered as a suitable epitope by secondary-structure predictions; and (ii) a fusion protein overproduced in Escherichia coli expressing the pRC13 cDNA. Immunoblot analysis showed that, when purified by antigen affinity chromatography, both types of antibody recognized a single band corresponding to ACC oxidase. Superimposition of Calcofluor white with immunofluorescence labeling, analysed by optical microscopy, indicated that ACC oxidase is located at the cell wall in the pericarp of breaker tomato and climacteric apple (Malus x domestica Borkh.) fruit. The apoplasmic location of the enzyme was also demonstrated by the observation of immunogold-labeled antibodies in this region by both optical and electron microscopy. Transgenic tomato fruits in which ACC-oxidase gene expression was inhibited by an antisense gene exhibited a considerable reduction of labeling. Immunocytological controls made with pre-immune serum or with antibodies pre-absorbed on their corresponding antigens gave no staining. The discrepancy between these findings and the targeting of the protein predicted from sequences of ACC-oxidase cDNA clones isolated so far is discussed.

Amino Acid Oxidoreductases↗
PubMed1986

[Culture of Aspergillus parasiticus in apple juice. I. The influence of sodium benzoate and potassium sorbate on fungal growth and aflatoxin biosynthesis].

Sodium benzoate or potassium sorbate (100, 200, 300 and 400 mg/l) were added to cultures of Aspergillus parasiticus NRRL 2999 on apple juice (from syrup) and incubated quiescently at 25 degrees C for 3, 6, 9, 12 or 15 days. The cultures were analyzed for pH, mycelial dry weight and accumulation of aflatoxin B1 and G1. The initial pH of 2.5 remained constant in all instances throughout the incubation period. Sodium benzoate, at all concentrations, suppressed fungal growth and stimulated the biosynthesis of G1, whereas little influence was exerted upon the accumulation of B1. Potassium sorbate stimulated fungal growth at 100 mg/l, while at all concentrations it considerably inhibited toxin production (no detectable amounts of B1 and 3 to 5 times less G1 than in controls). The concentration of G1 surpassed that of B1 without exception.

Aflatoxins↗
PubMed1987

[Culture of Aspergillus parasiticus in apple juice. II. The effect of butylhydroxyanisole (BHA) and butylhydroxytoluol (BHT) on fungal growth and aflatoxin biosynthesis].

Aspergillus parasiticus (NRRL 2999) was incubated in apple juice (from syrup), quiescently at 25 degrees C for up to 15 days in the presence of butylated hydroxyanisole (BHA) or butylated hydroxytoluene (BHT) at concentrations of 100, 200, 300, or 400 mg/l. Mycelial dry weight, pH and concentration of aflatoxin B1 and G1 were measured every 3 days with the initial pH of 2.5 remaining unchanged in all samples. BHA suppressed fungal growth and toxin accumulation during the observed incubation period. However, a concentration-dependent growth delay and earlier peaks of toxin accumulation suggested environmental adaptation of the mould. BHT (from 200 mg/l onwards), led to growth inhibition by about 25% (solubility limit of BHT lies between 200 and 300 mg/l), and at a concentration of 200 mg/l, it led to a reduction of toxin accumulation by approximately 45%. At 100 mg/l, however, BHT stimulated aflatoxin production (1.90 times more G1 and 6.65 times more B1 than in the controls). At all tested concentrations of BHA or BHT, as well as in the controls, the accumulation of aflatoxin G1, without exception, surpassed that of B1.

Aflatoxin B1↗
PubMed1988

Effect of irradiation on the patulin content and chemical composition of apple juice concentrate.

The influence of ionizing radiation on the patulin content of apple juice concentrate was investigated. The results indicated that patulin, at an initial concentration of about 2 mg/kg, disappeared after irradiation of the concentrate with doses as low as 2.5 kGy. For lower doses, the extent of patulin degradation was proportional to the absorbed dose. Irradiation of the concentrate with doses sufficient for patulin disappearance did not change the titratable acidity, the content of reducing sugars and carbonyl compounds or the amino acid composition. The content of ascorbic acid slightly decreased and the colour of the concentrate brightened. The intensity of the patulin absorption spectra after irradiation of mycotoxin in aqueous solutions decreased.

Amino Acids↗
PubMed1977

Determination of benomyl, carbendazim and 2-aminobenzimidazole (2-ab) in plant materials. Part I: Apples, red-currants, grapes, kale and sugar beets.

Benomyl and Carbendazim are widely used fungicides with systemic activity. In the Federal Republic of Germany the legal limits of Benomyl and Carbendazim lie between 0.1 and 7 ppm dependent on the substrate. A method for the determination of Benomyl and Carbendazim in apples, red-currants, grapes, kale, and sugar beets was developed. Benomyl and Carbendazim are extracted with ethyl acetate, saponified and determined as 2-aminobenzimidazole by thin-layer chromatography. The detection limits lie between 0.02--0.08 ppm, recoveries amount to 87% at 0.5-ppm levels.

Benomyl↗
PubMed1993

Automated determination of total polyphenols in apple juice.

An automated method of total polyphenols analysis in apple juice using the Folin-Ciocalteu reagent is presented. Chlorogenic acid was selected as the standard since a similar kinetic behaviour, standard-sample, was observed. A straightforward method with good reproducibility (CV < 1%), sampling frequency (48 h-1) and correlation with the traditional batch method (r = 0.9924) is proposed.

Beverages↗