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At least 1,027 records · Page 57Linked to original sources
PubMed2006

Estrogens, selective estrogen receptor modulators, and a selective estrogen receptor down-regulator inhibit endothelial production of tissue factor pathway inhibitor 1.

BACKGROUND: Hormone therapy, oral contraceptives, and tamoxifen increase the risk of thrombotic disease. These compounds also reduce plasma content of tissue factor pathway inhibitor-1 (TFPI), which is the physiological inhibitor of the tissue factor pathway of coagulation. The current aim was to study if estrogens and estrogen receptor (ER) modulators may inhibit TFPI production in cultured endothelial cells and, if so, identify possible mechanisms involved. METHODS: Human endothelial cell cultures were treated with 17beta-estradiol (E2), 17alpha-ethinylestradiol (EE2), tamoxifen, raloxifene, or fulvestrant. Protein levels of TFPI in cell media and cell lysates were measured by an enzyme-linked immunosorbent assay, and TFPI mRNA levels were assessed by quantitative PCR. Expression of ERalpha was analysed by immunostaining. RESULTS: All compounds (each in a concentration of 10 nM) reduced TFPI in cell medium, by 34% (E2), 21% (EE2), 16% (tamoxifen), and 28% (raloxifene), respectively, with identical inhibitory effects on cellular TFPI levels. Expression of TFPI mRNA was principally unchanged. Treatment with fulvestrant, which was also associated with down-regulation of secreted TFPI (9% with 10 nM and 26% with 1000 nM), abolished the TFPI-inhibiting effect of raloxifene, but not of the other compounds. Notably, the combination of 1000 nM fulvestrant and 10 nM raloxifene increased TFPI secretion, and, conversely, 10 nM of either tamoxifen or raloxifene seemed to partly (tamoxifen) or fully (raloxifene) counteract the inhibitory effect of 1000 nM fulvestrant. The cells did not express the regular nuclear 66 kDa ERalpha, but instead a 45 kDa ERalpha, which was not regulated by estrogens or ER modulators. CONCLUSION: E2, EE2, tamoxifen, raloxifene, and fulvestrant inhibited endothelial production of TFPI by a mechanism apparently independent of TFPI transcription.

Cells, Cultured↗
PubMed1999

Anaesthesia for day care surgery, patient selection, evaluation, preoperative preparation and selection of drugs.

Paediatric patients are a challenge to the anaesthetist because of their specific differences in behaviour, physiology, pharmacology, congenital anomalies and pathology. The number of day care paediatric patients show a steady increase. It is important that anticipated anaesthetic problems are solved or prevented by a good preoperative evaluation and preparation. Adequate psychological preparation and parental presence on induction and recovery have been shown to be a very good, if not the best premedication especially for toddlers. Relaxation of fluid restriction has led to more cooperation of the paediatric patient. Provided physicians, anaesthetists, surgeons and nurses share the same positive view on day care surgery, the facility will be greatly appreciated by both children and their parents.

Ambulatory Surgical Procedures↗
PubMed1979

[Action of grisin on the variability of an Acinomyces griseus culture and the use of the same antibiotic as a selection agent in the selection of active variants].

The effect of grisin on survival and variation of Actinomyces griseus producin grisin was studied. The efficiency of various concentrations of grisin on induction of variation according to the feature of the antibiotic production was compared. A possibility of increasing the productivity of strain. VNIIGenetics-115 by the use of the mutations of resistance to grisin is shown.

Anti-Bacterial Agents↗