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At least 1,027 records · Page 57Linked to original sources
PubMed1987

Vascular endothelial markers of the human thoracic duct and lacteal.

Factor VIII-related antigen (F8) and Ulex europaeus lectin (UEL) are accepted markers for human blood vessel endothelium. However, disagreement exists as to whether lymphatic vessels stain for F8, and accordingly this study was undertaken to address this issue. Moreover, another vascular endothelial marker, angiotensin converting enzyme (ACE) was also examined in lymphatics. Segments of human thoracic duct and portions of small bowel containing lacteals with post-mortem intervals of less than 15 hours, were removed at autopsy and fixed in B5 or formalin. The specimens were processed routinely and sections examined by indirect immunohistochemical techniques for F8 (Dako Corp.), ACE and for UEL (EY Lab). F8, UEL, and ACE positivity was uniformly found in thoracic ducts and lacteals; however, the staining intensity was less in lymphatic vessels with F8 and UEL than with comparable arteries or veins. ACE staining intensity, on the other hand, was similar in blood vessels and lymphatics. Both formalin and B5 fixation preserved antigenicity; however, background staining was greater with B5 fixation whereas tissue staining was slightly more intense with formalin fixation.

Antigens↗
PubMed1995

[Secondary plate osteosynthesis of open fractures of the lower extremity--still a therapeutic alternative!?].

During the time period from May 1, 1990 to July 31, 1993, a total of 102 tibial and 144 femur fractures were treated in the Department of Traumatology of the University of Bonn. 45 patients with 25 open tibial and 22 open femur fractures were treated according to a regimen including primary stabilization, usually in an external fixator, soft tissue reconstruction and delayed open reduction and internal fixation using an A0-plate. A majority of the patients were involved in motor vehicle accidents, leading to multiple injuries in 26 instances. The complication rate of 17% is comparable to the reports following intramedullary stabilization. Only three chronic infections, following one grade two open femur fracture, one grade two and one grade three open tibial fracture, were seen. Bony union was achieved after 24 weeks. It is shown that the use of the A0-plate in the treatment of open fractures of the femur and tibia offers not only logistic advantages but represents a viable alternative for the delayed stabilization of these injuries.

Adult↗
PubMed1993

The effects of aging on fixed DNA.

Agarose-gel electrophoretic analysis was carried out to study the effect(s) of aging on fixed naked DNA as well as on the DNA part of fixed chromosomes. Results show that aging of fixed DNA produces alterations in molecular size; the alteration is more effective in naked DNA than in the DNA part of fixed chromosomes; and the alteration occurs to a greater extent in air-dried DNA than in DNA aged in fixative. Since B-mercaptoethanol is capable of preventing DNA alterations induced by air-drying, oxidative processes are invoked for explaining these findings as well as the effect(s) of aging on fixed cytological preparations.

Animals↗
PubMed1999

Non-coating fixation techniques or redundancy of conductive coating, low kV FE-SEM operation and combined SEM/TEM of biological tissues.

Non-coating fixation methods, in particular the tannic acid/arginine/osmium tetroxide procedure, are employed for a number of reasons on the guinea-pig organ of Corti hair cell stereocilia glycocalyx and the imprints of the stereocilia at the bottom side of the tectorial membrane, and on the rat and cat intestinal epithelial microvilli glycocalyx and mucus-producing goblet cells. These methods are used firstly to confirm that non-coating prepared specimens can be embedded for TEM observation at 60-100 kV without loss of detail information, and these images can be compared with cryo-FE-SEM images of the same structure/tissue. Secondly, they show that specimens treated according non-coating techniques become optimally preserved and electrically conductive, so that no external conductive coating is required. In this way a comparison of images of subsequent fresh fracture faces is possible without a decrease in information on detail, which otherwise could happen after subsequent coating layers required after standard fixation. Thirdly, they show that non-coating methods can be used quite well with low accelerating voltages because the osmium-tannic acid complex in the specimen surface produces a large number of backscattered and secondary electrons in the surface layer, showing in particular surface phenomena. Fourthly, they show that with an optimal non-coating preservation, in combination with a well-balanced pre-fixation mixture, preparation artefacts due to extraction and even dehydration and drying are minimized. This is compared with images of the organ of Corti hair cells treated with a so-called three-aldehyde pre-fixation mixture, which causes disrupted stereocilia to cling onto the bottom side of the tectorial membrane.

Animals↗
PubMed1997

Quantitative comparison of immunohistochemical staining intensity in tissues fixed in formalin and Histochoice.

Formaldehyde fixatives have traditionally been used to preserve tissues as they impart excellent morphological preservation. Formaldehyde fixes tissue by cross linking, a process which can reduce the antigenicity of tissue and weakens the intensity of immunohistochemical stains. Preliminary studies have shown that Histochoice tissue fixative offers equal or greater staining intensity than neutral buffered formalin (NBF). This study compares these fixatives quantitatively and presents the results in unambiguous statistical terms. Tissue samples were collected, bisected, and fixed in NBF or Histochoice. The sections were stained with a total of 21 antibodies, and color images were collected. The hue, saturation, and value were determined for each positive pixel and an ANOVA performed. Small differences in hue were noted in 8 of 21 cases. Histochoice-fixed tissue gave a greater mean saturation than NBF with 57.1% of the antibodies tested. No significant difference in the saturation was detected in 28.6% of the cases; NBF gave higher mean saturation levels with only 14.3% of the antibodies. Histochoice-fixed tissue was found to give lower values in 66.7% of cases than those prepared with NBF, indicating darker staining. These results show that Histochoice produces staining intensity that is comparable, and in many cases superior, to formalin.

Analysis of Variance↗