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PubMed1984

A structural comparison of guinea pig thyroid and fat TSH receptors by photoaffinity labelling.

TSH receptors from guinea pig thyroid and epididymal fat have been covalently crosslinked to 125I-labelled TSH conjugated to N-hydroxysuccinimidyl-4-azidobenzoate. Analysis by SDS-PAGE and autoradiography showed bands corresponding to TSH subunits (Mr 14 000) and intact TSH (Mr 28 000; subunits crosslinked) and two at higher Mr separated by 14 000. The latter bands represented one or two subunits of TSH crosslinked to a subunit of the TSH receptor with Mr 57 000 (fat) or 60 000 (thyroid). These Mr values were reduced by trypsin treatment to 43 000 and 50 000, respectively. Analysis under nonreducing conditions showed that both fat and thyroid receptors have a second disulphide linked subunit of Mr 30 000.

Adipose Tissue↗
PubMed1984

Photoaffinity labelling of the nucleoside transporter of cultured mouse lymphoma cells.

Nitrobenzylthioniosine (NBMPR), a potent and specific inhibitor of nucleoside transport, is bound reversibly by high affinity sites on nucleoside transporter proteins of erythrocyte membranes and, upon photoactivation, NBMPR molecules become covalently bonded to the sites. This study showed that [3H]NBMPR molecules reversibly bound to intact S49 and L5178Y mouse lymphoma cells became covalently bound upon exposure to UV light. Electrophoretic analysis of plasma membrane fractions from the labelled cells showed that 3H was present in polypeptides which migrated as a major band with an apparent Mr of 45000-65000.

Affinity Labels↗