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PubMed1978

Dermatophagoides farinae antigen skin test sensitivity in Wisconsin.

Fifty consecutive patients with allergic asthma and/or rhinitis in Madison. Wisconsin, were tested intradermally with a house dust extract (1:100) and with a Dermatophagoides farinae antigen (1:2000). The close relationship between the two skin test results (mite and house dust) suggests that both contain the same cross-reacting antigens and that mites make a major contribution to the antigens in house dust in Wisconsin.

Antigens↗
PubMed1997

Iron and virulence of Haemophilus ducreyi in a primate model.

BACKGROUND AND OBJECTIVES: The pathogenesis of chancroid is currently unclear. There are discrepancies between the virulence of Haemophilus ducreyi in the human and animal experiments. Iron availability influences the virulence of many pathogens. The objective of this study was to investigate the role of iron in virulence of H. ducreyi. STUDY DESIGN: Intradermal inoculation of the primate Macaca mulatta was studied as an animal model for pathogenesis of chancroid. This model then was used to study the influence of iron availability on virulence of H. ducreyi. Eleven strains of H. ducreyi with proven avirulence in the rabbit intradermal test and one virulent control strain were used. Two of the strains had been shown to cause ulcers in a human inoculation experiment. Strains were suspended in fluid with different iron compounds before intradermal injection. Other monkeys were treated with injectable iron before inoculation with bacteria suspended. RESULTS: All but one of the test strains produced a chancroid-like ulcer from which H. ducreyi could be isolated. The minimal ulcerative dose was 10(5) cfu. Iron in the injection fluid decreased this dose 10-fold. In animals pretreated with intramuscular iron, the minimal ulcerative dose decreased to 10(4) cfu and all eleven strains became ulcerative. CONCLUSIONS: The availability of iron increases virulence of H. ducreyi. The iron pretreated primate model is useful for the study of virulence factors of H. ducreyi because of its ability to produce lesions with strains that avirulent in other animal models but pathogenic to humans.

Animals↗
PubMed1979

The role of mite allergen in chronic urticaria.

Fifty-two patients with chronic urticaria were tested intradermally with mite allergen (Dermatophagoides farinae). None had any past history of any other atopic diseases. Thirty of the 52 patients gave positive skin tests to mite allergen. A good correlation between skin sensitivity to mite allergen and in vitro mite antigen-induced histamine release from leucocytes was observed. These facts suggest that a mite may be an important role in chronic urticaria.

Allergens↗
PubMed1987

Histological study on the fate of haptenated epidermal cells injected intradermally in guinea pigs.

The percutaneous administration of in vitro haptenated epidermal cells (EC) has become established as a procedure to produce contact sensitivity (CS) in experimental animals for routine use. The cells have also been found to elicit a significant delayed-type skin reaction by intradermal test in the animals sensitized by painting the skin with the hapten. The fate of 2,4-dinitrophenylated (DNP) isogeneic epidermal cell suspensions (EC) injected intradermally was investigated histologically in intact or 2,4-dinitrochlorobenzene (DNCB)-sensitized strain 13 guinea pigs to study the role of the cells in CS. DNP-EC were found to proliferate actively in the dermis and formed EC nests with central keratinization and then elicited inflammatory reaction associated with necrosis of the epidermal structures 7 days after injection in the intact animals. DNP-EC injected intradermally into the animals which had received and reacted against DNCB underwent a suppression of EC proliferation. These findings are discussed in relation to the role of the haptenated EC in CS.

Animals↗
PubMed1997

Occupational asthma and rhinitis caused by ash (Fraxinus excelsior) wood dust.

An 18-year-old man who worked in a furniture factory reported rhinitis and asthma when he was exposed to ash wood dust. Monitoring of the patient's peak expiratory flow rate (PEFR) when off work and at work showed increased variations of PEFR at work. Basal PC20 methacholine was 1.41 mg/ml. A bronchial provocation test (BPT) with a 1:1000 w/v ash wood dust extract induced a dual asthmatic response with a 7.5-fold increase of nonspecific bronchial responsiveness. Intradermal testing with ash wood extract elicited a positive immediate response. IgE antibodies against ash wood were found in the patient's serum with a RAST value of 0.57 PRU/ ml. Similar skin tests, BPT, and RAST with ash wood dust performed in control patients were all negative. All the studies performed suggest that our patient had occupational rhinitis and asthma caused by exposure to ash wood dust in which a type I immunologic mechanism was implicated.

Adolescent↗
PubMed2006

Studies on diagnostic potential of secretory antigens of Mycobacterium bovis.

Culture filtrate proteins (CFPs) of M. bovis were produced by culturing the organism for a period of 3 weeks. These CFPs elicited a good delayed hypersensitivity (DTH) reaction in cattle and guinea pig. However, CFPs showed least cross-reactivity when tested in guinea pigs sensitized with M. bovis BCG and M. phlei. CFPs were found to be well recognized by T-cells of single intradermal test (SIDT) positive cattle and were also sero-reactive. Lymphocyte transformation assay revealed the culture filtrate antigens as major T-cell antigens. CFPs induced a good interferon gamma release as compared to conventional antigen, PPD, thereby highlighting their diagnostic capabilities.

Animals↗
PubMed1989

[Anaphylaxis caused by lidocaine].

A case of a severe anaphylactic reaction in a 30 year old man after 2 ml of 1% lidocaine injected subcutaneously is reported. It consisted in pruritus, urticaria, hypotension (Pasys: 50 mmHg), tachycardia (170 b.min-1) and a coma of short duration. The patient recovered after 1 mg adrenaline injected subcutaneously. The patient's history included a less severe but similar accident, but no manifestations of atopy. The total IgE, two months after the accident, were at the upper limit of normal. A human basophil degranulation test was positive for lidocaine and negative for other amide local anaesthetic agents and preservatives. Similarly, lidocaine was the only molecule giving a positive reaction with intradermal testing. Skin sensitivity to histamine was negative. Although for several authors true allergy to lidocaine is questionable, the case reported here can be added to the other 47 published since 1961.

Adult↗
PubMed2001

Delayed hypersensitivity skin testing to mumps and Candida albicans antigens is normal in middle-aged HTLV-I- and-II-infected U.S. cohorts.

It has been reported that human T cell lymphotropic virus (HTLV)-I-infected persons in Japan have decreased delayed hypersensitivity skin test reactivity to tuberculin purified protein derivative (PPD), but HTLV-I- or -II-infected persons do not generally develop opportunistic infections. We administered standardized intradermal testing with PPD, mumps, and Candida albicans antigens to 31 HTLV-I, 48 HTLV-II, and 143 seronegative subjects in the United States. Reactivity at 48 hr was compared among the three groups. Response rates to PPD were very low in all subjects. Fifty-five percent of seronegative subjects did not react to mumps antigen, compared with 55% of HTLV-I [adjusted odds ratio (OR) = 0.79, 95% confidence interval (CI) 0.27-2.33] and 38% of HTLV-II (OR = 0.73, 95% CI 0.33-1.64). Fifty-one percent of seronegatives did not react to Candida albicans antigen, compared with 34% of HTLV-I (OR = 0.37, 95% CI 0.15-0.93) and 46% of HTLV-II (OR = 0.71, 95% CI 0.34-1.52). Anergy was present in 33% of seronegatives, 28% of HTLV-I (OR = 0.60, 95% CI 0.20-1.78), and 19% of HTLV-II (OR = 0.56, 95% CI 0.22-1.44). HTLV-I- and -II-infected persons appear to have intact delayed hypersensitivity skin test responses to mumps and Candida albicans antigens.

Adult↗
PubMed1998

Delayed-type hypersensitivity to a nonionic, radiopaque contrast medium.

BACKGROUND: True allergic reactions to iodinated radiocontrast media are rare, and only a few well-documented cases of delayed-type hypersensitivity reactions caused by contrast media have been described. METHODS: We report a 61-year-old patient in whom percutaneous transluminal coronary angioplasty (PTCA) was performed with iopamidol, a nonionic contrast medium. Seven days later, the patient developed generalized maculopapular exanthema. Repeated patch tests with several iodinated agents were performed. RESULTS: A first patch test with iopamidol was positive. Repetition of the patch tests showed positive results to iopamidol as well as to iohexol and ioversol, two other nonionic contrast media, but not to other iodinated substances. Three months later, PTCA was repeated, and iopamidol was used again. Despite premedication, pruritic macular exanthema developed 1 day later. Whether iopamidol or trometamol -- an additive substance in the contrast medium -- was causative could not be determined, since a third set of patch tests was negative. CONCLUSIONS: Delayed-type hypersensitivity reactions to iodinated contrast media are rare. We recommend that patients with delayed exanthematous reactions undergo patch or intradermal tests with different contrast media and their additives, and that readings be performed immediately and later at days 2 and 3.

Angioplasty, Balloon, Coronary↗
PubMed2001

Lack of allergic cross-reactivity to cephalosporins among patients allergic to penicillins.

There are some contradicting data about clinical allergic cross-reactivity to cephalosporins among patients who have had a previous allergic reaction to penicillins. The purpose of this study was to assess the safety of administering cephalosporins to penicillin-allergic patients. The diagnosis of penicillin allergy was made by positive skin tests to penicillin reagents and/or provocation tests with the penicillin suspected of causing the allergic reaction. To assess the clinical tolerance to cephalosporins, 41 well-characterized penicillin allergic patients diagnosed by positive skin tests and/or provocation tests were challenged with three cephalosporins that do not share the same side chain to the penicillin that induced the reactions: cephazoline, cefuroxime and ceftriaxone. Skin prick and intradermal tests with all cephalosporins tested were negative. All penicillin-allergic patients tolerated therapeutic doses of the three cephalosporins tested (cephazoline, cefuroxime and ceftriaxone) without any ill effect. These results indicate that the risk of suffering from an allergic reaction on administering cephalosporins to penicillin-allergic patients seems to be very low, provided that cephalosporins with a different side chain to the penicillin responsible for the allergic reaction are used.

Adult↗
PubMed2005

[A role of individual tuberculin diagnosis in the formation of registry group VI].

The authors studied the use of comprehensive individual tuberculin diagnosis (scarification skin test, intradermal titer) in the differential diagnosis of the nature of tuberculin sensitivity in children referred for examinations by physiological pediatricians for the diagnosis of a turn of tuberculin tests and the formation of registry group VIA. The use of this procedure was found to avoid the hyperdiagnosis of a turn of tuberculin tests in as high as 55.8%, to improve the diagnosis of hyperergic reactions to tuberculin, and to detect an occult false hyperergic reaction. Moreover, there is a possibility of observing the changes in individual sensitivity in Registry Group 0 children, which contributes to their more timely transfer to Group VIA. Analyzing the results shows that the use of comprehensive tuberculin diagnosis promotes the optimization of a system of preventive measures aimed at preventing tuberculosis in children.

Adolescent↗
PubMed1968

Tissue culture method for toxigenicity testing of Corynebacterium diphtheriae.

A method for toxigenicity testing of Corynebacterium diphtheriae in tissue cultures was developed. Results were obtained by comparing destruction of the monkey kidney or, preferably, rabbit kidney monolayer by 0.1 ml of the C. diphtheriae culture in Elek's broth containing 20% rabbit serum with the appearance after the addition of 0.2 ml of a mixture of the C. diphtheriae culture and diphtheria antitoxin. The mixture of C. diphtheriae broth culture and 10 antitoxin units per ml was incubated for 1 hr at room temperature before it was added to the tissue cultures which were then incubated as long as 5 days; most results, however, were read in 72 hr. Elek's broth medium was superior to heart infusion broth for toxin production by C. diphtheriae. Addition of 20% rabbit serum improved toxin production in either broth. Numerous toxigenic and atoxigenic C. diphtheriae cultures were tested for toxigenicity in primary rabbit and monkey kidney tissue cultures. If properly controlled, this in vitro method appeared to have an advantage over the in vitro agar gel method; its results were comparable with the rabbit intradermal test. With the wider use of tissue cultures in most laboratories, we believe that the tissue culture method for toxigenicity would be more economical and easier to perform than the animal intradermal method.

Animals↗
PubMed1984

Anergy secondary to sepsis in rats. Relation to outcome.

A rat model was developed to determine if delayed hypersensitivity skin tests during early peritonitis would predict outcome. Presensitized rats were simultaneously tested intradermally with keyhole-limpet hemocyanin and given four types of fecal bacteria and 10% barium sulfate intraperitoneally. Rats were divided into four groups according to inoculum dose. Skin tests were read at 24 hours in survivors and correlated with death and abscesses during the next 19 days. In the two groups with greatest mortality, 35% of anergic rats died, compared with 0% of reactive rats. In the three groups with most abscesses, anergic rats had more abscesses than those that reacted. Overall, 90% of anergic rats died or had abscesses v only 10% of reactors. We concluded that the outcome of untreated peritonitis is determined in the first 24 hours; anergy at that time predicts death or abscess formation.

Abscess↗
PubMed1980

Contact sensitivity to aluminium in a patient hyposensitized with aluminium precipitated grass pollen.

Standard patch testing of a patient with eczema revealed positive reactions to the aluminium discs used for testing. Patch tests with various aluminium allergens were positive in the patient and negative in 53 controls. The patient was having hyposensitization for hay-fever with an aluminium precipitated allergen. Intradermal testing with aluminium was positive in the patient and negative in nine controls. The eczema disappeared when hyposensitization was discontinued. The possibility of primary sensitization by the hyposensitization procedure is discussed.

Adolescent↗
PubMed2006

Current understanding of contrast media reactions and implications for clinical management.

Iodinated contrast media (CM) are an integral part of modern diagnostic medicine. Although these agents are considered to be relatively safe, adverse effects in the form of allergy-like reactions occur in a significant number of exposed patients. These reactions may be divided into immediate and delayed responses. Immediate (within 1 hour of administration) anaphylactic reactions range from urticaria and angioedema to laryngeal oedema, hypotension and even death. Delayed reactions to CM occur from 1 hour to 1 week after administration and usually have mostly cutaneous manifestations. History of prior CM reactions and atopy predispose patients to CM reactions. Despite intense research into the pathogenesis of the immediate anaphylactoid responses, new evidence shows that true IgE type I hypersensitivity mediation occurs only in rare, severe cases. The aetiology appears to be multifactorial in most individuals. There is strong evidence to conclude that type IV hypersensitivity is responsible for the delayed reactions to CM. Although switching to non-ionic agents significantly reduces the incidence of immediate reactions to CM, there is little consensus regarding corticosteroid prophylaxis in high-risk individuals. Skin testing and provocative challenges also provide little security. Therefore, physicians must be better prepared to treat immediate anaphylactoid responses. Preventing delayed CM reactions is best performed with patch and delayed intradermal testing in those with a history of prior reactions, although false-negative results have been reported. Corticosteroids and antihistamines may be required for treatment. Until newer agents are developed that negate these issues, healthcare providers must strive to better understand the risk factors associated with CM reactions, as well as the available prophylactic and treatment options.

Anaphylaxis↗
PubMedDate not supplied

Erythema multiforme from betalactams with positive cutaneous tests.

We report the case of a woman who after taking ampicillin and amoxicillin developed two severe cutaneous reactions with generalized erythema and maculopapular rash, which later turned into blisters with subsequent desquamation. She was clinically diagnosed with erythema multiforme syndrome after positive delayed cutaneous tests with benzylpenicillin, amoxicillin and ampicillin allowed for a more specific diagnosis. There are very few references to patients with drug reactions to betalactams who have positive skin prick or intradermal test but show delayed hypersensitivity, as was the case in our patient.

Amoxicillin↗
PubMed2005

Drug patch testing in systemic cutaneous drug allergy.

Patch testing with the suspected compound has been reported to be helpful in determining the cause of a cutaneous adverse drug reaction (CADR) and in studying the pathophysiological mechanisms involved. The main advantages of drug patch tests are that they can be done with no hospital surveillance because they induce only rarely adverse reactions and that any commercialized form of a drug can be used. In contrast, intradermal tests can be performed only with injectable forms or with a pure and sterile form of the drug. It is advised to perform drug patch tests during the 6 months following the CADR as we do not know whether positive results will persist. Due to the possibility that a low concentration might yield false negative results, drug patch tests have to be performed with rather high concentrations of the commercialized form of the drug, mostly diluted at 30% in petrolatum and/or in water. For some drugs and severe CADR, it is necessary to tests with lower concentrations or in other vehicles. Drug patch tests are positive in ca. 32-50% of patients who have developed a CADR. The clinical relevance of drug patch tests depends on the clinical features of the CADR (valuable in testing generalized eczema, systemic contact dermatitis, maculopapular rash, acute generalized exanthematous pustulosis, fixed drug eruption) and on the involved drug. As false positive results can be observed, it is always necessary to consider the relevance of any positive drug patch test. Their specificity and their negative predictive value have not been yet determined.

Animals↗
PubMed1964

[Epidemiological studies of Clonorchis sinensis prevailed in the peoples of Kyungpook Province]

The author studied the infection rates of Clonorchiis sinensis in the inhabitants of Kyungpook Province, and surveyed infestation rates of Clonorchis metacercarias in the twelve kinds of fresh water fishes for the purpose of the eradication. plan of clonorchiasis of Kyungpook Province. The examination was continued from April 1960 to October 1962 with 24,252 inhabitants ranging from four months to over sixty aged. All the family were tested intradermally with Clonorchis antigen and then confirmed the eggs by M.G.L. technique. The results of the examination were summarized as follows. I. The infection rate of the inhabitants; 1) In the people tested, 27.7 per cent was infected with Clonorchis sinensis. 2) There were no remarkable differences in the infection rates among each Districts; River side inhabitants(Yungchun-Gun 23.6 percent, Andong-Gun 27.4 %), paddy field inhabitants(Yungchun-Gun 23.6 %, Sangchu-Gun 29.1 %) and mountain side inhabitants (Chungsong-Gun 23.8 %, Yungyang-Gun 27.7%). However, the infection rates of the river side peoples were slightly higher than that of the districts far from the river. 3) The infection rate of the male was 34.98 percent (4,628 out of 12,230), while the female was 18.96 per cent (2,090 out of 11,022). 4) The maximum infection rate of the male group was observed in the 35-39 age group (73.8 %) and in female, 55-59 age group (34.l percent) respectively, however it was gradually decreased in the senile group. II. The infection rates of the family group; 1) Those infected in both husband and wife were 28 percent(357 out of 1,289), husband only was 52 percent (672 out of 1,289) and wife only was 4 percent (50 out of 1,289). 2) The children of the infected parents revealed higher morbidity than that of the non-infected parents. 3) The children of the infected father revealed much morbidity than that of the mother's side. On the contrary, children's morbidity rate of the infected widow was higher than that of the widower. III. The infestation rates of fresh water fishes; 1) The metacercaria of Clonorchis sinensis was found in the flesh exclusive of the Misgurnus anguillicaudatus and Zacco platypus in the 11 kinds of fresh water fishes. 2) The maximum infestation rate of 55.8 percent was revealed at Pseudorasbora parva and next to it was Sarcocheilichthys wakiyae of 5l.7 percent. 3) The number of metacercaria in flesh was decreased as compared to the previously examined level.

Journal Article↗