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At least 1,027 records · Page 57Linked to original sources
PubMed1991

An innovation in flow cytometry data collection and analysis producing a correlated multiple sample analysis in a single file.

The problems associated with rapid analysis and interpretation of data from multicolor immunofluorescence panels have been a formidable barrier to their routine use. Using present flow cytometry concepts, a panel of 11 tubes each containing multiple phenotypic markers or controls requires postdata acquisition manipulation of many multiparameter histogram and listmode files. We have developed a method that compresses all of the information from such a panel into a single listmode data file during run time. A single data file is used to record the entire phenotypic analysis for a particular patient or series within an experiment. This is accomplished by the incorporation of a tube identifier parameter (TIP) as well as the fluorescence and light scatter parameters normally collected. The TIP can then be used for gating discrimination of any tube or set of tubes within a panel. When the TIP is correlated with the PRISM parameter the entire patient phenotypic image can be represented within a single two-parameter histogram we have called a phenogram. This phenogram can be generated in real time, providing on-line preprocessing of a complex multicolor experiment. By examining the image created by the phenogram it is possible to rapidly flag abnormalities such as incorrect gating. This procedure was carried out on an EPICS Elite flow cytometer in its standard configuration with the addition of hardware to provide an input for the TIP.

Data Collection↗
PubMed1996

Aspirin or acetaminophen? A comparison from data collected by the Spanish Drug Monitoring System.

To characterize and compare the toxicity profiles of aspirin and acetaminophen when used by large populations, all records of adverse drug reactions to these drugs reported to the Spanish Drug Monitoring System from 1982 to 1991 were analyzed. According to these data, aspirin-induced reactions were more serious than those reactions to acetaminophen; the severity of all the reactions in both cases was associated with time of exposure and with the accumulated dose administered. For GI reactions to aspirin no association was found between severity and time of exposure or dose, although an association with age was found. There were no differences between the proportions of deaths, malformations, and renal damage recorded for either of the two groups. Proportions of hematological and hepatic disturbances were greater with acetaminophen. Adverse drug reaction data for aspirin and acetaminophen from spontaneous reporting seem to be consistent with data coming from observational studies.

Acetaminophen↗
PubMed1986

[Industrial drug-surveillance. Spontaneous notification: data collection, survey].

The importance of post-marketing drug surveillance has often been stressed. To detect new undesirable side-effects it seems more appropriate to improve spontaneous reporting of such effects. The main stages of spontaneous reporting are listed, with detailed information on his first and second stages: data recording and validation.

Data Collection↗
PubMed1998

[From "reporting" to "surveillance": documentation of abnormalities within the scope of perinatal data collection--a sensitive instrument for monitoring new technologies?].

Analysis of the Hamburg perinatal quality assurance programme shows a dramatic increase in congenital abnormalities of the bones, joints and muscles from 45.29 per 10,000 live births in 1990 to 106.32 in 1994. Comparisons with other perinatal quality assurance programme data reveal almost the same trend. In fact, there is no real increase in these specific congenital abnormalities, but there is an effect, which can be explained by the introduction of ultrasound hip screening in infancy signalling overdiagnostics due to the new technology. This example demonstrates that the perinatal quality assurance programme is a suitable instrument for registering congenital abnormalities and monitoring new technologies and interventions. These results support the proposal by the association of the leading state health officers (AGLMB) to use the already well-established quality assurance programmes which are analysed on state level for a systematic nation-wide documentation and "surveillance" of congenital abnormalities. In addition, it becomes clear that surveillance, understood as a further development of health reporting, offers an opportunity to effect both an assessment and a better orientation in respect of health policies.

Bias↗
PubMed1985

A new EDP assisted dietary survey employing a simple and concise data collection method.

The aim of the work was a diet analysis practice both accurate and simple to be used in daily applications. The subject records in real time his intake by employing the portion of food as unit of measure. Through the recipes, which may be obtained in deferred time, data are then reorganized by EDP to match food composition data of literature. A food composition table management system was developed which avoids vacancies of data: nine different sources from literature were used. The fact that the subjects records in a simple and concise way his intakes permits prolonged investigation: slight alterations of the diet are possible without changing his food preferences and with minimum reduction of compliance.

Computers↗
PubMed2001

Overexpression, purification, crystallization and data collection of 3-methylaspartase from Clostridium tetanomorphum.

3-Methylaspartase (E.C. 4.3.1.2) catalyses the reversible anti elimination of ammonia from L-threo-(2S,3S)-3-methylaspartic acid to give mesaconic acid as well as a slower syn elimination from the (2S,3R)-epimer, L-erythro-3-methylaspartic acid. The anti-elimination reaction occurs in the second step of the catabolic pathway for glutamic acid in Clostridium tetanomorphum. The reverse reaction is of particular interest because the addition of ammonia to substituted fumaric acids is highly stereoselective and gives highly functionalized amino acids. The mechanism of the transformation is unusual and of considerable interest. 3-Methylaspartase from C. tetanomorphum has been overexpressed and purified from Escherichia coli. Crystals of the enzyme have been obtained by sitting-drop vapour diffusion. Two native data sets have been collected, one in-house on a rotating-anode generator to 3.2 A and one at the European Synchrotron Radiation Facility to 2.0 A. A 2.1 A data set has been collected on a crystal of selenomethionine protein. Combining the data sets identify the space group as P2(1)2(1)2, with unit-cell parameters a = 110.3, b = 109.9, c = 67.2 A, alpha = beta = gamma = 90 degrees. The asymmetric unit contains two monomers with 42% solvent. A self-rotation function indicates the presence of a twofold axis, consistent with a biological dimer.

Ammonia-Lyases↗
PubMed2004

Data collection, maintenance, and analysis for rheumatic disease research.

With the increasing availability of inexpensive, powerful computers and software, clinicians and academics are constructing or thinking about constructing rheumatic disease outcomes databases. This article describes concepts, methods, and problems in database construction and analysis based on the experience of the US National Data Bank for Rheumatic Diseases.

Data Collection↗
PubMed2005

Evaluation of the DotMap algorithm for locating analytes of interest based on mass spectral similarity in data collected using comprehensive two-dimensional gas chromatography coupled with time-of-flight mass spectrometry.

Comprehensive two-dimensional gas chromatography coupled with time-of-flight mass spectrometry (GC x GC-TOF-MS) is a highly selective technique ideal for the analysis of complex mixtures. The instrument yields an abundance of data, with complete mass spectral scans at every time point in the GC x GC separation space. The development and application of appropriate tools for data mining is essential in making sense of the wealth of information available. An algorithm for locating analytes of interest based on mass spectral similarity in GC x GC-TOF-MS data, called DotMap, has been previously reported and is rigorously evaluated herein. A thorough investigation into the performance characteristics of DotMap, including the performance near the limit of detection and dynamic range of the algorithm as well as the capacity of the algorithm to deal with peak overlap, is investigated using jet fuel as a complex sample matrix. For instance, the algorithm can successfully identify a spiked compound at the single microg/ml level in a jet fuel sample with an overlapping interferent. The performance of the DotMap algorithm in situations with very limited mass spectral selectivity, specifically in the evaluation of spectra from isomer compounds, as well as the ability to tune DotMap results to provide the location of a specific analyte or of a class of compounds is demonstrated. The DotMap algorithm is demonstrated to be a sensitive tool that is useful in the analysis of complex mixtures and which possesses the capacity to be easily "tuned" to discern the location of analytes of interest.

Algorithms↗
PubMed2004

Handheld-assisted field data collection for occupational risk assessment.

A computerized handheld procedure is presented in this paper. It is intended as a database complementary tool, to enhance prospective risk analysis in the field of occupational health. The Pendragon forms software (version 3.2) has been used to implement acquisition procedures on Personal Digital Assistants (PDAs) and to transfer data to a computer in an MS-Access format. The data acquisition strategy proposed relies on the risk assessment method practiced at the Institute of Occupational Health Sciences (IST). It involves the use of a systematic hazard list and semi-quantitative risk assessment scales. A set of 7 modular forms has been developed to cover the basic need of field audits. Despite the minor drawbacks observed, the results obtained so far show that handhelds are adequate to support field risk assessment and follow-up activities. Further improvements must still be made in order to increase the tool effectiveness and field adequacy.

Computers, Handheld↗
PubMed1994

Data collection strategies for patient-reported information.

The U.S. health care transition demands increased accountability for medical care. This has contributed to increased interest in documenting medical outcomes, including improvements in health-related quality of life and treatment satisfaction. These data can only be obtained by asking patients directly about their current health state, perception of well-being, and satisfaction with care. Systematic collection of patient-reported data is often poorly done because its demands are underestimated and inadequately supported. Rigorous quality assurance is critical in any clinical trial or treatment delivery evaluation program.

Clinical Trials as Topic↗
PubMed2006

Crystallization, data collection and phasing of the molybdate-binding protein of the phytopathogen Xanthomonas axonopodis pv. citri.

Xanthomonas axonopodis pv. citri ModA protein is the ABC periplasmic binding component responsible for the capture of molybdate. The protein was crystallized with sodium molybdate using the hanging-drop vapour-diffusion method in the presence of PEG or sulfate. X-ray diffraction data were collected to a maximum resolution of 1.7 A using synchrotron radiation. The crystal belongs to the orthorhombic space group C222(1), with unit-cell parameters a = 68.15, b = 172.14, c = 112.04 A. The crystal structure was solved by molecular-replacement methods and structure refinement is in progress.

ATP-Binding Cassette Transporters↗
PubMed1993

Drug use in pregnancy: a comparative appraisal of data collecting methods.

We have compared the reliability of the information about drug therapy and pregnancy retrieved by interviewing patients with that distilled from pharmacy records. In the initial phase of each interview we used the internationally accepted open-ended technique, and extended this with an indication-oriented set of questions and then a set of specific drug-oriented questions. These data were then compared with those from pharmacy records on dispensing for the same patients during their pregnancy. The results suggest that if drug consumption during pregnancy is evaluated by interview, one should not restrict oneself to open-ended questions but should include indication-oriented and, when appropriate, drug-oriented questions. Such specific questions offer the opportunity of detecting the use of over-the-counter medication and of constructing drug use/complaint profiles. By contrast, pharmacy records will give better information in case of long recall periods and in patients with multiple and/or repeated drug use. Investigators should use the complementary elements of both techniques where appropriate.

Adult↗