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PubMedDate not supplied

Microbial contamination of toothbrushes and their decontamination.

PURPOSE: The objective was to determine the level of contamination of toothbrushes by mutans streptococci using microbiological identification, to access the bacterial contamination using scanning electron microscopy (SEM) and to evaluate the efficacy of two toothbrush disinfectants. METHODS: Nineteen children used their toothbrushes once a day, for five consecutive days. The toothbrushes were then immersed into disinfectant solutions for 20 h: Group I--0.12% chlorhexidine gluconate; Group II--1% sodium hypochlorite; Group III--sterile tap water. They were then placed into test tubes containing CaSa B, for 3 to 4 days at 37 degrees C. The number of MS cfu was counted and the toothbrushes were submitted to SEM analysis. RESULTS: There was no bacterial growth in Groups I and II; Group III showed MS growth (range, 21 to 120 cfu). Scanning electron microscopy showed biofilm formation on toothbrush bristles. CONCLUSION: Immersion in 0.12% chlorhexidine gluconate and 1% sodium hypochlorite are efficient methods for toothbrush disinfection.

Anti-Infective Agents, Local↗
PubMed2000

[Problems of disinfection and decontamination in the biological protection and prevention of contagious diseases].

The problems of biological protection of the general population and military staff against biological hazard sources, primarily against biological terrorism and military threat underlie works on the design of new disinfectants having a universal spectrum of antimicrobial action and less ecological hazard than currently available ones. Elimination of biological hazard require effective chemical disinfectants that can completely inactivate microbes of unknown etiology as rapidly as possible and that cause the minimum damage to environmental objects. The disinfectant PFK of various modifications is evaluated in terms of the requirements of that ecological and chemical concept aimed at testing chemicals entering the environment.

Decontamination↗