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Investigation on solvent casting films of surfactant-encapsulated clusters.

The surfactant-encapsulated cluster (SEC) composed of a hydrophobic dimethyl dioctadecyl ammonium (DODA) shell and an encapsulated hydrophilic polyoxoanion core can form casting films. The structure of the casting film is influenced by evaporation rates of organic solvent. When the casting films are prepared by slow evaporation of chloroform, the alkyl chains are considered to possess a partial interdigitation, and the interdigitated length is 1.6 nm. The casting film structure is characterized by scanning force microscopy (SFM), Fourier transformation infrared (FT-IR), wide-angle X-ray diffraction, and differential scanning calorimetry (DSC).

Journal Article↗

Luminal morphology of small arterial vessels in the contracted spleen, studied by scanning electron microscopy of microcorrosion casts.

Unique luminal configurations exhibited by small arterial vessels in contracted spleens of dog and cat were studied by means of vascular corrosion casts examined by scanning electron microscopy. Concertina-like pleating was seen in casts of trabecular arteries/arterioles, whereas within lymphatic nodules arteriolar casts lacked pleating and were smooth and uniformly cylindrical (as were all small arterial vessels in distended spleens). Morphological details of arterial vessels observed in histological sections indicated that pleating is not due to contraction of specially arranged vascular smooth muscle but to overall shortening of trabecular arterial vessels, caused by contraction of longitudinal smooth muscle in trabeculae. Another phenomenon observed in casts from contracted spleens was an almost complete "pinching-off" of many arteriolar lumens; histological evidence indicated that this is due to contraction of vascular smooth muscle, which selectively diverts flow away from certain regions of the organ. Also noted was a markedly convoluted, tortuous configuration of arterioles (penicilli) in the red pulp of contracted spleens.

Animals↗

Peripilar keratin casts: a study with the scanning electron microscope.

Two cases of Peripilar keratin casts are presented and the results of a Scanning EM investigation are discussed. The surface of the casts is very irregular and disordered. In the underlying structure of the casts the characteristic image of a "sponge" is observed. The cuticle of the hair beneath is slightly flaky, showing fissures. The hair surface facing the peripilar casts is normal.

Child, Preschool↗

CAST liposuction: an alternative to brachioplasty.

The aging female with excess arm fat and poor skin tone frequently refuses a brachioplasty scar due to permanent detectability. Traditional deep liposuction localized to the posterolateral aspect of the arm frequently leaves sagging, wrinkled skin. Circumferential para-Axillary Superficial Tumescent (CAST) liposuction was developed to maximize skin retraction and create regional harmony by preparatory compartment magnification with dilute lidocaine and epinephrine followed by circumferential treatment of the arm and adjacent areas utilizing superficial and/or subdermal liposuction. Early CAST liposuction results in patients with moderate fat and excess skin revealed excellent skin retraction. CAST liposuction was then offered as the first of two stages to patients with excess fat and poor skin tone to avoid or shorten the brachioplasty scar. Twenty-six patients underwent CAST liposuction with 9-22 months follow up. Only two patients (7.7%) eventually required brachioplasty. Although postoperative seromas were frequent (38.5%) and preexisting skin wrinkling usually returned, the final result is acceptable to the vast majority of patients (84.6%) who refuse a brachioplasty scar.

Adult↗

Mapping the dominant wound healing and soft tissue regeneration QTL in MRL x CAST.

We have used a mouse ear punch model and the QTL (quantitative trait loci) mapping technique to identify genes that are responsible for soft tissue regeneration. In the early studies, we have identified several QTL and have shown that the inheritance of ear healing was additive in one cross (MRL x SJL), and recessive in another cross (DBA x 129). Because CAST mice are genetically distinct and have a different genetic background, CAST would facilitate the identification of common and novel QTL when crossed with common inbred lines. We made a cross between super healer MRL and poor healer CAST and collected ear punch phenotype and marker genotype data from F(2). Ear punch healing exhibited a dominant mode of inheritance in this cross. There were three main QTL on Chromosomes 4, 9, and 17, and two suggestive QTL on Chromosomes 1 (new) and 7. Taken together, these QTL accounted for about 29% of total F2 variance of MRL x CAST. Compared with another study using the same cross, we found a totally different set of QTL. Two QTL interactions were identified by a full QTL model: Chromosomes 4 x 17 and 9 x 17; the latter reached to a statistical level at p < 0.05. These interactions explained about 4% of the F2 phenotypic variance. We conclude that soft tissue regeneration is controlled by multiple genes and locus vs. locus interactions.

Animals↗

Cerebral cast angiography as an aid to medicolegal autopsies in cases of death after adult cardiac surgery.

Due to an increase in age of the patient population in cardiac surgery, cerebral complications are increasing in frequency, also as a cause of death. In order to reveal cerebral pathology associated with a fatal outcome after cardiac surgery, we re-evaluated the cast angiographs and medico-legal autopsy documents of 144 adult cardiac surgery subjects over a 7-year period. Special attention was paid to the ability of post-mortem cast angiography to aid in diagnosing cerebral pathology. The autopsy detected new ischemic cerebral lesions in 29 (20%) cases, of which 22 (15.3%) were recent infarcts, and 7 were cases of anoxic brain damage. Of the recent cerebral infarcts, 12 were associated with cerebral artery thrombosis, 4 showed multiple lesions, and the remaining 6 were small single infarcts. In addition, one subject had an intracerebral hemorrhage and 72 (50%) cerebral edema. By cast angiography, the leakage of contrast medium in the case of intracerebral hemorrhage and stenoses of intracranial and cervical arteries could be well demonstrated and also revealed 17 (77%) of the 22 recent cerebral infarcts. It was found to be suitable for detecting recent brain infarcts associated with main cerebral artery thrombosis, with a sensitivity of 92% (11 out of 12 cases), but was less sensitive in showing small recent infarcts with a sensitivity of 60% (6 out of 10 cases) and inferior for the older ones where none of the 6 cases were detected. Filling defects caused by cerebral edema were difficult to differentiate from technical errors and were encountered in 7 (4.8%) cases. A significant predictor for the 29 recent ischemic brain lesions was perioperative hypotension. The immediate cause of death was most often of cardiac (83%) and cerebral (14%) origin. In 14 cases, cerebral damage was considered to be an additional cause of death. The use of cerebral post-mortem cast angiography should be recommended, especially for its excellent ability to visualize intravascular pathology such as arterial stenoses and thromboses, with a 92% sensitivity in showing new main cerebral artery thromboses, before likely distortion of the vascular anatomy by dissection.

Adult↗

Correlation of left ventricular angiographic casts and biplane left ventricular volumetry in infants and children.

To calculate left ventricular (LV) volumes from biplane cineangiography obtained in nonstandard views, regression equations were developed from LV casts of known volume. Volumes were calculated by the area-length method from casts ranging from 1.4 to 48.9 ml obtained from 30 postmortem cases with heart disease. The casts were divided into 2 groups: group I (n = 17) with abnormal and group II (n = 13) with normal right ventricular hemodynamics. Biplane cinegrams were taken in the anterolateral, anterior and long axial oblique, hepatoclavicular and sitting-up projections. The true volume of each cast was determined from its weight and specific gravity. In both groups, excellent correlations were obtained between measured and true volumes (r = 0.92 to 0.99) in all projections, although each projection overestimated the true volume (slope value less than 1). The regression equations obtained from group I were significantly different from those in group II in all views (p less than 0.025 to 0.05), with smaller mean differences and standard errors of the estimate. These data support the concept that right ventricular hemodynamics influence ventricular septal position and, therefore, LV geometry and measured volumes. Appropriate regression equations are required to allow volume calculation from multiple projections.

Cardiac Volume↗

Correlation of right ventricular volume using axial angulated ventriculography to known right ventricular cast volumes in infants and children with congenital heart disease.

To calculate right ventricular (RV) volumes from biplane cineangiography obtained in nonstandard views, regression equations were developed from RV casts of known volume. Volumes were calculated using Simpson's rule from casts ranging from 2 to 42 ml from 25 postmortem specimens with various congenital heart diseases. The casts were divided into 2 groups: group 1 (n = 15) with abnormal or group 2 (n = 10) with normal RV hemodynamic measurements. Biplane cinegrams were taken in the anterolateral, anterior and long axis oblique, hepatoclavicular and sitting up projections. The true volume of each cast was determined from its weight and specific gravity. Excellent correlations were obtained between measured and true volumes (r = 0.92 to 0.96) in all projections, although each projection overestimated the true volume (slope value less than 1). The regression equations obtained from group 1 were not statistically different from those in group 2 in any view. Although the application of different regression equations is required in measuring RV volumes by multiple angulated angiography, these regression equations appear not to be affected by the hemodynamic state of the ventricle. These results are important in assessing RV volume in pediatric patients with congenital heart disease using axial angulated ventriculography.

Cineangiography↗

Tooth position measurements on dental casts using holographic images.

A new accurate method for comparing tooth positions on dental casts at different stages is described. Holograms of casts were prepared with a helium-neon gas laser. For evaluation of tooth movements, a cast and hologram representing different time points were superimposed by means of an adjustable x-y stage. Occlusal surface detail was used as a reference for the superimposition. Positional changes of the upper incisors were detected as discrepancies between the cast and the hologram image.

Adult↗

Operational performance of the Reflex Metrograph and its applicability to the three-dimensional analysis of dental casts.

The purpose of the present study was to investigate the applicability of a new instrument (the Reflex Metrograph) to the three-dimensional analysis of dental casts. In this system, a dental cast, placed in front of a semireflecting mirror, creates an identical image behind the mirror. The measurement of a point is made by aligning a light source behind the mirror in coincidence with the image of a specific point on the object. The light source is carried on a three-dimensional slide system, and coordinates are digitized for sampling by the computer. Measurement accuracy of the instrument was evaluated for three measuring points determined on a maxillary dental cast placed in six different locations. In addition, differences in the performance skills of ten operators were evaluated. The results indicate that digitization of a point in three planes of space can be undertaken without direct contact of the object and that a consistent measurement accuracy can be obtained, regardless of differences in the location of the cast relative to the mirror or in the anatomic shape of the measured areas. In addition, operators with no previous experience can determine specific points to an accuracy of +/- 0.1 mm, with a minimal amount of training. Finally, measurement by the same operator for a pooled sample is recommended in order to avoid interoperator measurement error when determining linear distances.

Computers↗

Experimental study of pulsatile and steady flow through a smooth tube and an atherosclerotic coronary artery casting of man.

In vitro investigation of pulsatile and steady flows through a smooth, straight circular tube and a diseased human coronary artery cast was conducted with sugar-water solutions simulating the viscosity of blood. Time averaged pressure drops for pulsatile flows measured in the circular tube over a Reynolds number ranging from 50 to 1,000 were found to be identical to those for steady flows in the same tube, both of which were in excellent agreement with the Poiseuille flow prediction. For the polyurethane case (# 124) made from a human main coronary with significant but 'non obstructive' diffuse atherosclerotic disease, pressure drops for steady flows were found to be greater than Poiseuille flow predictions by a factor of 3-8 in the physiological Reynolds number range from about 100 to 400. Pulsatile flows in the same artery cast resulted in additional 30% increases in time averaged pressure drops, and thus flow resistance, compared to the steady flow data. Steady and pulsatile flow data measured in a straight, axisymmetric model of cast # 124 showed considerably smaller increases in flow resistance than those observed in # 124 casting.

Arteriosclerosis↗

The effects of die relief agent on the retention of full coverage castings.

The effect of a commercial die relief agent on the retention of castings cemented with a zinc phosphate, a zinc polycarboxylate, or a modified zinc oxide-eugenol cement was studied. Forces required to dislodge unspaced castings from tooth preparations were higher when zinc phosphate cement was the luting medium. Die relief agents resulted in a 32% reduction in the forces required to dislodge castings cemented with zinc phosphate. Data for the other cements did not differ statistically. Shear strength-tensile ratios of the cements indicate that the high shear strength provided by zinc phosphate cement may be negated by the cementation of oversize castings fabricated from relieved dies.

Crowns↗

Mounting casts on a new plasterless plane line articulator.

A technique of mounting casts on a plane line articulator without the aid of plaster or artificial stone has been described. The advantages of this technique are: The dimensional change errors inherent in a plaster or dental stone mounting are avoided. Casts may be routinely mounted and articulated in less than 3 minutes. Casts may be removed and returned to their original position with precise reliability. The disadvantages include the lack of an adjustable horizontal condylar guidance and the lack of an anterior guide pin or anterior guidance. Further studies should be directed toward determining the accuracy of remounting casts on the instrument.

Dental Articulators↗

Remount casts for removable partial dentures made in a laboratory.

A remount cast for a removable partial denture can be made in the laboratory by making an elastomeric impression of the prosthesis on the cast after processing but before removing it from the cast. After the prosthesis is finished and polished it is placed in the impression, undercuts are blocked out, and a mounting cast is made.

Dental Occlusion↗

Effect of prolonged ultrasonic instrumentation on the retention of cemented cast crowns.

Occasionally the need arises to remove restorations that have been cemented. Several ultrasonic tips have been developed to aid in removing cemented restorations. Earlier studies of the effects of ultrasonic vibration reveal detrimental effects on amalgam surface and marginal integrity and, at short instrumentation periods (2.5 minutes) in vitro, no significant effect on the retention of cemented cast crowns. Twenty identically machined metal dies had nonprecious crowns waxed, cast, and fitted. Each casting was cemented twice with glass ionomer and zinc phosphate cement. Each cement was tested for the effects of vibration on retention. The cements were mixed according to manufacturer's specifications, placed under 5 kg load, then placed in 37 degrees C water and contained in a humidor. Retention was tested with a 4202 Instron machine to determine the forces needed to overcome retention. Retention ranged from a high of 125.36 kg for glass ionomer without vibration to a low of 19.11 kg for zinc phosphate vibrated for 12 minutes. The use of vibration is considered an advantageous adjunct to other cast restoration removal devices.

Cementation↗

Evaluation of the accuracy of cast reorientation to a surveyor by prosthodontic residents.

This study investigated the accuracy of a group of evaluators in reestablishing the orientation of a tripoded master cast to a surveyor instrument. Three prosthodontic residents served as evaluators to reestablish the tilt of a previously tripoded cast with three known reference coordinates. The distance from the known to the reference point was recorded for the three points in four trials by a judge with a machinist height gauge. Analysis of variance revealed a significant difference between the evaluator cast position and the known reference position. The investigation demonstrated +/- 0.2 mm errors at each of the three locations; 0.3-degree angulation errors occurred in reestablishing the tilt of the master cast in relation to the surveyor.

Analysis of Variance↗

Principles for selecting interocclusal records for articulation of dentate and partially dentate casts.

Minimizing the orientation error of the working and opposing casts on the articulator should reduce the extent of intraoral adjustment of restorations before their placement. This article defines and reviews principles such as the tripod of vertical support (three widely spaced occlusal contacts) and adequate horizontal stability, which enable opposing dental casts to be held together in a stable and reproducible manner at the time the mandibular cast is mounted to the articulator. During the restoration of the dentate or partially dentate patient, where restoration is to be coincident with pretreatment maximum intercuspation, the goal of the interocclusal record is to provide stability and/or support for the casts. The types of interocclusal records are organized into categories and designs based upon the remaining vertical support and horizontal stability of the dentition. The clinical indications, limitations, design, and composition of a variety of records for each of these categories are reviewed and discussed.

Dental Articulators↗

Index to reposition the metal framework accurately on the altered cast.

The relieved portion of a master cast below the major connector of a cast removable partial denture framework can be filled with dental stone to act as a third point of reference when the framework is reseated in an altered cast procedure. With this simple procedure, an index is formed that will allow accurate repositioning of the metal framework on the altered cast.

Calcium Sulfate↗