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Variability in the interpolated twitch torque for maximal and submaximal voluntary contractions.

The superimposed twitch technique is frequently used to study the degree of motor unit activation during voluntary effort. This technique is one of the preferred methods to determine the activation deficit (AD) in normal, athletic, and patient populations. One of the limitations of the superimposed twitch technique is its variability under given contractile conditions. The objective of this research was to determine the source(s) of variability in the superimposed twitch force (STF) for repeat measurements. We hypothesized that the variability in the AD measurements may be caused by the timing of the twitch force relative to the onset of muscle activation, by force transients during the twitch application, by small variations in the actual force from the nominal target force, and by variations in the resting twitch force. Twenty-eight healthy subjects participated in this study. Sixteen of these subjects participated in a protocol involving contractions at 50% of their maximal voluntary contraction (MVC) effort, whereas the remaining 12 participated in a protocol involving contractions at 100% of their MVC. Doublet-twitch stimuli were superimposed onto the 50 and 100% effort knee extensor muscle contractions, and the resting twitch forces, voluntary knee extensor forces, and STFs were then measured. The mean resting twitch forces obtained before and after 8 s of 50% of MVC were the same. Similarly, the mean STFs determined at 1, 3, 5, and 7 s into the 50% MVC were the same. The variations in twitch force were significantly smaller after accounting for the actual force at twitch application than those calculated from the prescribed forces during the 50% MVC protocol (P < 0.05). Furthermore, the AD and the actual force showed statistically significant negative correlations for the 50% MVC tests. The interpolated twitch torque determined for the maximal effort contractions ranged from 1 to 70%. In contrast to the protocol at 50% of MVC, negative correlations were only observed in 5 of the 12 subjects during the 100% effort contractions. These results suggest that small variations in the actual force from the target force can account for the majority of the variations in the STFs for submaximal but not maximal effort contractions. For the maximal effort contractions, large variations in the STF exist due to undetermined causes.

Adult↗

BOLD coherence reveals segregated functional neural interactions when adapting to distinct torque perturbations.

In the natural world, we experience and adapt to multiple extrinsic perturbations. This poses a challenge to neural circuits in discriminating between different context-appropriate responses. Using event-related fMRI, we characterized the neural dynamics involved in this process by randomly delivering a position- or velocity-dependent torque perturbation to subjects' arms during a target-capture task. Each perturbation was color-cued during movement preparation to provide contextual information. Although trajectories differed between perturbations, subjects significantly reduced error under both conditions. This was paralleled by reduced BOLD signal in the right dentate nucleus, the left sensorimotor cortex, and the left intraparietal sulcus. Trials included "NoGo" conditions to dissociate activity related to preparation from execution and adaptation. Subsequent analysis identified perturbation-specific neural processes underlying preparation ("NoGo") and adaptation ("Go") early and late into learning. Between-perturbation comparisons of BOLD magnitude revealed negligible differences for both preparation and adaptation trials. However, a network-level analysis of BOLD coherence revealed that by late learning, response preparation ("NoGo") was attributed to a relative focusing of coherence within cortical and basal ganglia networks in both perturbation conditions, demonstrating a common network interaction for establishing arbitrary visuomotor associations. Conversely, late-learning adaptation ("Go") was attributed to a focusing of BOLD coherence between a cortical-basal ganglia network in the viscous condition and between a cortical-cerebellar network in the positional condition. Our findings demonstrate that trial-to-trial acquisition of two distinct adaptive responses is attributed not to anatomically segregated regions, but to differential functional interactions within common sensorimotor circuits.

Adaptation, Physiological↗

Different predictions by the minimum variance and minimum torque-change models on the skewness of movement velocity profiles.

We investigated the differences between two well-known optimization principles for understanding movement planning: the minimum variance (MV) model of Harris and Wolpert (1998) and the minimum torque change (MTC) model of Uno, Kawato, and Suzuki (1989). Both models accurately describe the properties of human reaching movements in ordinary situations (e.g., nearly straight paths and bell-shaped velocity profiles). However, we found that the two models can make very different predictions when external forces are applied or when the movement duration is increased. We considered a second-order linear system for the motor plant that has been used previously to simulate eye movements and single-joint arm movements and were able to derive analytical solutions based on the MV and MTC assumptions. With the linear plant, the MTC model predicts that the movement velocity profile should always be symmetrical, independent of the external forces and movement duration. In contrast, the MV model strongly depends on the movement duration and the system's degree of stability; the latter in turn depends on the total forces. The MV model thus predicts a skewed velocity profile under many circumstances. For example, it predicts that the peak location should be skewed toward the end of the movement when the movement duration is increased in the absence of any elastic force. It also predicts that with appropriate viscous and elastic forces applied to increase system stability, the velocity profile should be skewed toward the beginning of the movement. The velocity profiles predicted by the MV model can even show oscillations when the plant becomes highly oscillatory. Our analytical and simulation results suggest specific experiments for testing the validity of the two models.

Biomechanical Phenomena↗

Direct pelletization in a rotary processor controlled by torque measurements. III. Investigation of microcrystalline cellulose and lactose grade.

The aim of the present study was to investigate the use of different grades of microcrystalline cellulose (MCC) and lactose in a direct pelletization process in a rotary processor. For this purpose, a mixed 2- and 3-level factorial study was performed to determine the influence of the particle size of microcrystalline cellulose (MCC) (approximately 60 and 105 microm) and lactose (approximately 30, 40, and 55 microm), as well as MCC type (Avicel and Emcocel) on the pelletization process and the physical properties of the prepared pellets. A 1:4 mixture of MCC and lactose was applied, and granulation liquid was added until a 0.45 Nm increase in the torque of the friction plate was reached. All combinations of the 3 factors resulted in spherical pellets of a high physical strength. The particle size of MCC was found to have no marked effect on the amount of water required for agglomerate growth or on the size of the resulting pellets. An increasing particle size of lactose gave rise to more spherical pellets of a more narrow size distribution as well as higher yields. The MCC type was found to affect both the release of the model drug from the prepared pellets and the size distribution. Generally, the determined influence of the investigated factors was small, and direct pelletization in a rotary processor was found to be a robust process, insensitive to variations in the particle size and type of MCC and the particle size of lactose.

Cellulose↗

Comparison of muscle torque, balance, and confidence in older tai chi and healthy adults.

PURPOSE: The objectives of this cross-sectional study were to examine whether older Tai Chi practitioners had better knee muscle strength, less body sway in perturbed single-leg stance, and greater balance confidence than healthy older adults. METHODS: Tai Chi and control subjects (N = 24 each, aged 69.3 +/- 5.0 and 71.6 +/- 6.1 yr, respectively) were matched with respect to age, gender, height, weight, and physical activity level. Concentric and eccentric isokinetic tests of the subjects" dominant knee extensors and flexors were conducted at an angular velocity of 30 degrees.s(-1). Control of body sway was assessed in static double-leg stance and in single-leg stance perturbed by forward or backward platform perturbations. The Activities-specific Balance Confidence (ABC) scale was used to investigate subjects" balance confidence in daily activities. RESULTS: Tai Chi practitioners had higher peak torque-to-body weight ratios in concentric and eccentric isokinetic contractions of their knee extensors and flexors (P = 0.044). They manifested less anteroposterior body sway angles in perturbed single-leg but not static double-leg stance than did control subjects (P < 0.001). Tai Chi practitioners also reported significantly higher balance confidence score ratios (P = 0.001). Older adults" knee muscle strengths showed negative correlations with body sway angles in perturbed single-leg stance and positive correlations with ABC score ratios. Moreover, their body sway angles in perturbed single-leg stance were negatively correlated with their ABC score ratios (all P < 0.05). CONCLUSION: Our results demonstrate that long-term Tai Chi practitioners had better knee muscle strength, less body sway in perturbed single-leg stance, and greater balance confidence. Significant correlations among these three measures uncover the importance of knee muscle strength and balance control during perturbed single-leg stance in older adults" balance confidence in their daily activities.

Aged↗

Usefulness of mini-tracheostomy and torque controlled insertion of applicator in fractionated endobronchial brachytherapy.

Endobronchial brachytherapy was developed as effective treatment of endobronchial cancer and fractionated schedule is applied to decrease late toxicity. However, repeated bronchofiberscopy is onerous to the patient and restricts the treatment schedule itself. We applied mini-tracheostomy for a ready access route, and a torque controlled technique for easy insertion of the endobronchial applicator. Eight patients with tracheobronchial cancer invasion were treated with endobronchial brachytherapy of 18-30 Gy/3-5 fractions/1.5-2.5 weeks (median 24 Gy/4 fractions/2 weeks) at reference points of 5 mm from the bronchial surface. The averaged individual irradiation and single session times were 4 min and 24 min, respectively. There were no procedure-related complications. These technical improvements may facilitate flexible fractionated dose prescriptions.

Aged↗

Charnley low-frictional torque arthroplasty in patients under the age of 51 years. Follow-up to 33 years.

Between November 1962 and December 1990 a group of 1092 patients, 668 women and 424 men, under the age of 51 years at the time of surgery, underwent 1434 primary Charnley low-frictional torque arthroplasties and are being followed up indefinitely. Their mean age at operation was 41 years (12 to 51). At the latest review in June 2001 the mean follow-up had been for 15 years 1 month. Of the 1092 patients 54 (66 hips) could not be traced, 124 (169 hips) were known to have died and 220 (248 hips) had had a revision procedure. At a mean follow-up of 17 years and 5 months, 759 patients (951 hips) are still attending. In this group satisfaction with the outcome is 96.2%. The incidence of deep infection for the whole group was 1.67%. It was more common in patients who had had previous surgery (hemi- and total hip arthroplasties excluded), 2.2% compared with 1.5% in those who had not had previous surgery, but this difference was not statistically significant (p = 0.4). There were fewer cases of deep infection if gentamicin-containing cement was used, 0.9% compared with 1.9% in those with plain acrylic cement, but this was not also statistically significant (p = 0.4). There was a significantly higher rate of revision in patients who had had previous hip surgery, 24.8% compared with 14.1% in those who had not had previous surgery (p < 0.001). At the latest review, 1.95% are known to have had at least one dislocation and 0.4% have had a revision for dislocation. The indication for revision was aseptic loosening of the cup (11.7%), aseptic loosening of the stem (4.9%), a fractured stem (1.7%), deep infection (1.5%) and dislocation (0.4%). With revision for any indication as the endpoint the survivorship was 93.7% (92.3 to 95.0) at ten years, 84.7% (82.4 to 87.1) at 15 years, 74.3% (70.5 to 78.0) at 20 years and 55.3% (45.5 to 65.0) at 27 years, when 55 hips remained 'at risk'.

Adolescent↗

The effects of land vs. aquatic plyometrics on power, torque, velocity, and muscle soreness in women.

The purpose of this study was to compare changes in performance indicators (power, torque, and velocity) and muscle soreness between plyometric training on land and in water. Thirty-two college age women were randomly assigned to 8 weeks of an identical plyometric training program on land or in an aquatic setting. Performance indicators were assessed pretraining, midtraining, and posttraining. Muscle soreness (ordinal scale) and pain sensitivity (palpation) were assessed after a training bout (0, 48, and 96 hours) during the first week of training and when training intensity was increased (weeks 3 and 6). Performance indictors increased for both groups (pretraining < midtraining < posttraining, p < or = 0.001). Muscle soreness was significantly greater in the land compared to the aquatic plyometric training group at baseline and each time training intensity was increased, p = 0.01. Aquatic plyometrics provided the same performance enhancement benefits as land plyometrics with significantly less muscle soreness.

Exercise↗

Difference in isokinetic torque acceleration energy of the rotator cuff: competitive male pitchers versus male nonathletes.

Rotator cuff function is critical to the overhead athlete. Rotator cuff power is felt to be important in the overhead athlete during the throwing motion. Little research exists regarding torque acceleration energy (TAE) in overhead athletes. Twenty-five males were divided into 2 groups consisting of overhead athletes (pitchers) (n = 12) and nonoverhead athletes (controls) (n = 13). All participants were given a concentric velocity spectrum isokinetic test at speeds of 60 degrees (1.05 r), 180 degrees (3.16 r), and 300 degrees.s(-1) (5.26 r) to both the dominant and nondominant shoulder internal and external rotators. Significant differences were found for all internal rotator TAE scores (p = 0.000-0.016), at each of the 3 velocities, when comparing dominant to nondominant arms of both overhead athletes and nonoverhead athletes. Only 60 degrees.s(-1) (1.05 r) was found to be different during external rotation TAE testing of the overhead athletes (p = 0.027) but was not found in the control subjects. Post hoc analysis revealed no differences between dominant or nondominant TAE scores when comparisons were made between overhead athletes and controls. Results may reveal that power of the rotator cuff muscles may not be a critical component of the overhead throwing motion.

Adolescent↗

Acute effects of static stretching on peak torque in women.

The purpose of this study was to examine the effects of static stretching on concentric, isokinetic leg extension peak torque (PT) at 60 and 240 degrees.s(-1) in the stretched and unstretched limbs. The PT of the dominant (stretched) and nondominant (unstretched) leg extensors were measured on a calibrated Cybex 6000 dynamometer. Following the prestretching PT assessments, the dominant leg extensors were stretched using 1 active and 3 passive stretching exercises. After the stretching, PT was reassessed. The results of the statistical analyses indicated that PT decreased following the static stretching in both limbs and at both velocities (60 and 240 degrees.s(-1)). The present findings suggested that the stretching-induced decreases in PT may be related to changes in the mechanical properties of the muscle, such as an altered length-tension relationship, or a central nervous system inhibitory mechanism. Overall, these findings, in conjunction with previous studies, indicated that static stretching impairs maximal force production. Strength and conditioning professionals should consider this before incorporating static stretching in preperformance activities. Future studies are needed to identify the underlying mechanisms that influence the time course of stretching-induced decreases in maximal force production for athletes and nonathletes across the age span.

Adult↗

Acute effects of static stretching on peak torque and mean power output in National Collegiate Athletic Association Division I women's basketball players.

The purpose of this study was to examine the acute effects of static stretching on peak torque (PT) and mean power output (MP) during maximal, voluntary concentric isokinetic leg extensions at 60 and 300 degrees .s(-1) in National Collegiate Athletic Association Division I Women's Basketball players. Eleven members of a women's basketball team volunteered to perform maximal concentric isokinetic leg extensions at 60 and 300 degrees .s(-1) on a calibrated Biodex System 3 dynamometer. After the initial isokinetic testing, the dominant leg extensors were stretched using 1 unassisted and 3 assisted static stretching exercises. The poststretching isokinetic assessments were repeated at 5, 15, 30, and 45 minutes after the static stretching (post-5, post-15, post-30, and post-45). PT (N.m) and MP (W) were recorded by dynamometer software. The results indicated no stretching-related changes in PT (p = 0.161) or MP (p = 0.088) from pre- to poststretching for any of the testing intervals (post-5, post-15, post-30, and post-45). These findings indicated that the static stretching had no impact on PT or MP during maximal, voluntary concentric isokinetic muscle actions in collegiate women's basketball players. In conjunction with previous studies, these findings suggested that trained athletes may be less susceptible to the stretching-induced force deficit than untrained, nonathletes.

Adult↗

A multiplex PCR assay able to simultaneously detect Torque teno virus isolates from phylogenetic groups 1 to 5.

Torque teno virus (TTV) is a circular, single-stranded DNA virus that chronically infects healthy individuals of all ages worldwide. TTV has an extreme genetic heterogeneity which is reflected in its current classification into five main phylogenetic groups (1-5). Using specific PCR assays, it has been shown that many individuals are co-infected with TTV isolates belonging to different phylogenetic groups. Here, a multiplex PCR assay was developed, using five recombinant plasmids. Each plasmid carried an insert of different size issued from a TTV isolate belonging to a different group. The assay was able to simultaneously amplify DNAs of TTV isolates belonging to all five phylogenetic groups. Multiplex PCR was then tested satisfactorily on DNAs extracted from 55 serum samples (47 health care workers and 8 AIDS patients). All individuals but nine were infected with at least one TTV isolate. Co-infection with multiple isolates was found in 29/47 (62%) health care workers and in 8/8 (100%) AIDS patients. A number of discrepancies were observed when results obtained with three thermostable DNA polymerases were compared. For example, four TTV phylogenetic groups were detected in a particular serum sample by using one of the three DNA polymerases, whereas the other two enzymes were able to detect only three TTV groups. However, none of the three enzymes used could be broadly considered to be more efficient than the others. Despite its limitations, the assay described here constitutes a suitable tool to visualize the degree of co-infection of a given population, avoiding time-consuming experiments.

Acquired Immunodeficiency Syndrome↗

One-year monthly monitoring of Torque teno virus (TTV) in river water in Italy.

Torque teno virus (TTV) is prevalent worldwide in general populations but at present is not related with any specific pathology. Its presence in faeces and its remarkable environmental stability suggest the possibility of using it as an indicator of faecal contamination in the environment. To evaluate the waterborne spread of TTV and its possible relationship with human pathogen enteric viruses, water samples were collected monthly for a year (May 2004-April 2005) from a river receiving the effluent of the treatment plant of the city of Pisa, concentrated and assayed with bimolecular tests (PCR, RT-PCR). TTV was detected in three samples (25%) while 16% of samples were positive for enteroviruses, 33% for rotaviruses, 8% for noroviruses genotype 1 and 25% for noroviruses genotype 2. Only two TTV samples (June and January) were also positive for rotavirus and norovirus, respectively. The detection of TTV in water confirmed its possible faecal-oral route of transmission but data are still insufficient to draw conclusions about the role of TTV as a viral indicator.

Base Sequence↗

Root resorption on torqued human premolars shown by tartrate-resistant acid phosphatase histochemistry and transmission electron microscopy.

OBJECTIVE: To identify clastic cells on the root surfaces of torqued human premolars. MATERIALS AND METHODS: A continuous force of 600 cNmm was applied to upper first premolars in patients 13-16 years of age by using a precise biomechanical model with superelastic wires (NiTi-SE). The 28 teeth in 14 patients were divided into five groups (control [nonmoved], and moved for either 1, 2, 3, or 4 weeks) and processed for tartrate-resistant acid phosphatase (TRAP) histochemistry and transmission electron microscopy. RESULTS: Mononuclear TRAP-positive cells appeared at 2 weeks, where as large multinucleated TRAP-positive cells were numerous at 3 and 4 weeks. Ultrastructural examination revealed many clastic cells in contact with resorption lacunae. In addition, some cementoblast-like cells appeared secreting new cementum over previously resorbed lacunae. CONCLUSIONS: In general, resorption lacunae and the number of clastic cells, which increased with the duration of the applied force, were found on the cementum surface at the pressure areas. Some signs of cementum repair were also noticed, even with the maintenance of the level of the force.

Acid Phosphatase↗

Invertor vs. evertor peak torque and power deficiencies associated with lateral ankle ligament injury.

Strengthening of the evertor muscles is widely advocated as a key component of lateral ankle sprain rehabilitation, but our clinical observation of impaired invertor muscle performance among many patients suggested the need for this study of isokinetic performance deficiencies. Subjects were 30 physically active adolescents, ages 14-19 years, who had recently sustained a lateral ankle sprain or who had symptoms of chronic lateral ankle instability. Eversion/inversion testing was performed on a Biodex isokinetic dynamometer at speeds of 30 and 120 degrees/sec. Analysis of variance results demonstrated significantly greater (p < .05) invertor deficits than evertor deficits for both peak torque and average power at both tests speeds. The findings of this study suggest that a lateral ankle ligament injury may be associated with an invertor muscle performance deficiency, and that restoration of a normal evertor/invertor strength relationship may be accomplished through performance of an isotonic ankle strengthening program.

Adolescent↗

Torque responses in human quadriceps to burst-modulated alternating current at 3 carrier frequencies.

STUDY DESIGN: Independent group design, 1 group per carrier frequency, with subjects stratified by gender and systematically assigned to groups. OBJECTIVE: To determine the effect of the frequency of burst-modulated alternating current (AC) on the electrically induced torque (EIT) response of the quadriceps femoris muscle. BACKGROUND: Many studies have examined the influence of current amplitude and burst modulation on the EIT response. However, research is limited on the effects of carrier frequency on the EIT response of the quadriceps femoris. METHODS AND MEASURES: Twenty-three healthy subjects (mean age +/- SD, 22.7 +/- 1.7 years) volunteered for the study and were systematically assigned to 1 of 3 groups: group 1 (2500-Hz AC), group 2 (3750-Hz AC), and group 3 (5000-Hz AC). For normalization purposes, maximal voluntary isometric contraction (MVIC) force was assessed with a load cell prior to the application of 5 minutes of neuromuscular electrical stimulation (NMES). The EIT and current amplitude were recorded during each of the 5 minutes of NMES. RESULTS: The mean EIT ([EIT/MVIC] x 100) produced at equivalent current amplitudes when using the 2500-Hz carrier frequency was significantly greater than the mean EITs generated at 3750 Hz (P = .004) and 5000 Hz (P<.001), respectively. CONCLUSIONS: Burst-modulated alternating current delivered at 2500 Hz produced greater EITs than those generated at 3750 and 5000 Hz.

Adult↗

Effect of ankle position fixation on peak torque and electromyographic activity of the knee flexors and extensors.

The purpose of this study was to examine the effect of ankle position fixation on peak torque (PT) and electromyographic (EMG) activity of knee-joint muscles during isokinetic testing. Twelve female athletes performed isokinetic knee flexion and extension at 60 degrees and 180 degrees/s under two conditions: with the ankle fixed in a position of plantarflexion and with the ankle fixed in a position of dorsiflexion. Bipolar surface electrodes were placed on the vastus lateralis, vastus medialis, biceps femoris, medial hamstrings, and the lateral head of the gastrocnemius for determination of the root mean square of the EMG (rmsEMG) and the median frequency of the EMG (mfEMG). No significant differences in knee extensor PT were noted in either ankle position for each velocity tested. Significant differences were noted, however, in knee flexor PT (p < 0.05) at both 60 degrees and 180 degrees/s, with the greatest PT observed with the ankle fixed in dorsiflexion. Neither quadriceps, hamstrings, nor gastrocnemius rmsEMG activity was affected by ankle position; however, there was a significant difference in mfEMG for the gastrocnemius, with higher frequencies observed with the ankle fixed in plantarflexion (p < 0.01). These results suggest that ankle position effects knee flexor PT during open chain isokinetic movements. The reason for decreased knee flexor PT with the ankle fixed in plantarflexion is probably due to the gastrocnemius muscle being in a too shortened position, thereby preventing it from effectively producing force at the knee joint.

Adult↗

Knee extensor torque and perceived discomfort during symmetrical biphasic electromyostimulation.

The purpose of this study was to examine the effects of simultaneously delivering 2 channels of electromyostimulation (EMS) current using 2 different electrode arrangements. Ten men and 10 women university students had 4 reusable electrodes placed (2 proximal, 2 distal) medial and lateral on the quadriceps muscle group. Isokinetic voluntary peak torque (VPT) of the quadriceps was determined at 60 degrees x s(-1). A symmetrical biphasic square wave current was applied using 2 independent channels in either a parallel (P) or a crossed (X) electrode arrangement. Subjects increased the current until maximal tolerance was achieved. No significant differences in percent VPT or perceived discomfort (PD) were observed between men and women. Percent VPT was significantly greater using the X (57.2 +/- 11.3%) vs. the P (46.5 +/- 10.7%) pad placement; however, pad placement did not affect peak PD. Data from this study suggest that a 2-channel application of EMS using a crossed electrode arrangement provides greater knee extensor force without greater discomfort.

Adult↗