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PubMed2006

There is no relation between the fascicular and the endoneural blood vessel areas on the cervical vagus nerve of normotensive rats.

The aim of this study was to determine the existence of a relation between the fascicular area and total vessel area on the cervical vagus nerve of Wistar-Kyoto female rats. Animals aged 30, 180 or 360 days had their right vagus nerves prepared for epoxy resin embedding and light microscopy study. Semithin serial sections were studied every 100 microm of the longitudinal extension of the nerve. The fascicular area and the total endoneural vessel area were obtained for each nerve segment (proximal, middle and distal) and compared between segments and ages. The relation between fascicular area and endoneural vessel area was accessed by linear regression analysis and correlation coefficient calculations. No significant relationship between the two variables was detected in all nerves studied. This suggests that the entrance of blood vessels to the endoneural space is not accompanied by an increase in the fascicular area and this may have implications on the fiber density calculations since, in the presence of large vessels, this density can be underestimated if the vessel area is not discounted. The endoneural vessel area increased from proximal to distal in all groups but got smaller with ageing, suggesting that older animals may be more susceptible to nerve ischemia.

Aging↗
PubMed1999

Peri-implant tissues at submerged and non-submerged titanium implants.

The present experiment was performed to study the peri-implant tissue response to non-submerged (1-stage) and initially submerged (2-stage) implant installation procedures. 6 beagle dogs were used. All mandibular premolars and the 1st, 2nd and 3rd maxillary premolars were extracted. After 3 months of healing, 3 fixtures of the Astra Tech System were installed and submerged in the right (or the left) edentulous, premolar region in each of the 6 dogs. Radiographs were obtained immediately after fixture installation. In the radiographs, the distance between the abutment-fixture junction and the most "coronal" bone in contact with the implant surface was determined. 3 months later, abutments were connected to the initially submerged fixtures and another 3 fixtures of the same system were installed in the contralateral, edentulous premolar region. Abutments were, however, immediately connected to the newly-installed fixtures (non-submerged side; test side). The mucosal flaps were replaced, adjusted and sutured in such a way that the coronal portion of the abutments remained exposed in the oral cavity. A new set of radiographs were obtained from all 6 implant sites in each animal. A period of plaque control was initiated. Clinical examinations were performed and radiographs obtained from all implant sites after another 3 months and at the termination of the experiment. 9 months after the 1st fixture installation procedure, the animals were sacrificed, the mandibles were removed, and each implant region dissected. The most mesially-located implant sites were processed for ground sectioning. The remaining biopsies were processed and embedded in EPON. The histometric analysis included assessment of the vertical dimension of the marginal soft and mineralized peri-implant tissues. The ground sections were used for measurements describing (i) "bone to implant contact" and (ii) "bone density". It was observed that the mucosa and bone tissue that formed at implants placed in a non-submerged or a submerged procedure had many features in common. Thus, figures describing (i) the height of the mucosa, (ii) the length of the junctional epithelium and the height and quality of the zone of "connective tissue integration", (iii) the % of bone to implant contact as well as (iv) the density of the peri-implant bone, were similar in the submerged and the non-submerged groups. It is therefore suggested that a non-submerged (1-stage) installation technique may provide conditions for tissue integration that are similar to those obtained using a submerged (2-stage) approach.

Alveolar Process↗
PubMed1994

Cryofixation combined with physical dehydration for quantitative immunoelectron cytochemistry.

Common methods for preparing samples for immunoelectron microscopy involve glutaraldehyde fixation (GA) followed by chemical dehydration (CD) or cryofixation (CF) succeeded by physical dehydration, i.e., freeze drying (FD) or freeze substitution (FS). The effects of these techniques have been evaluated with regard to the sizes of epoxy resin embedded rat somatotrophic secretory granules as well as the immunolabeling densities over these granules. The measurements were performed by computerized image analysis using electron microscopy in transmission (TEM) and scanning transmission (STEM) modes, which allowed us to define the immunolabeling in detail. The embedded secretory granules showed the same diameters after GA (2 hr) with CD and GA (15 min) with CF and FS, but were smaller after CF-FS, and smallest after GA (15 min) with CF and FD. The highest labeling density appeared after GA (15 min) and physical dehydration, in particular after freeze substitution. Based on our STEM pictures a new factor for evaluating and interpreting immunolabeling of granules is introduced; the "accessible immunogold labeling surface." It defines the fraction of the epoxy resin surface that is labeled and varies with the preparation methods. By using this factor, an order of labeling densities/micron 2 over the accessible areas could be established for the different techniques: GA-CF-FS > CF-FS > GA-CF-FD > GA-CD. The high labeling after GA-CF-FS may be due to the combination of a large accessible area and accurate preservation of the antigenicity of the hormones in the granules.

Animals↗
PubMed1997

Regeneration of diaphyseal bone defects using resorbable poly(L/DL-lactide) and poly(D-lactide) membranes in the Yucatan pig model.

OBJECTIVES: To determine the effects of tubular resorbable polymer membranes on the healing of a segmental diaphyseal bone defect. DESIGN: A randomized prospective study using the minipig model. Animals were evaluated with in vivo roentgenograms on a biweekly basis until explanted at twelve weeks. SETTING: After surgery, animals were allowed unrestricted activity and weight bearing between twenty-four and forty-eight hours. ANIMALS: Fifteen yearling Yucatan minipigs. INTERVENTION: A 2.5- to 3.0-centimeter mid-diaphyseal defect was created in the middle third of the radius. Animals were assigned in groups of three to receive the following implants: (a) poly(L/DL-lactide), (b) poly(L/DL-lactide)-CaCO3, (c) poly(D-lactide), (d) poly(D-lactide)-CaCO3, and (e) an untreated defect. No adjunctive internal or external fixation was used as the ulna was left intact. MAIN OUTCOME MEASURES: The limbs were studied with in vivo anterior-posterior and lateral radiographs at biweekly intervals for the presence and pattern of bone formation. All limbs were explanted at twelve weeks postimplantation for methyl-methacrylate embedding and histologic and microradiographic study. RESULTS: The bone defects covered with membranes were completely reconstituted by six to eight weeks. Untreated defects healed with less bone formation and in a more disorganized pattern. Histologic evaluation of the implants demonstrated that the entire lumen of the implant was filled with bone, with some periosteal bone formation occurring on the outer surface of the membrane. There was direct apposition of bone onto the membrane surface or minimal fibrous tissue interposition between membrane and new bone. There was no foreign body or adverse reaction to the membrane. Untreated defects showed woven bone formation with clefts and irregularly shaped margins occupied by fibrous tissues or surrounding muscle tissues. CONCLUSIONS: This study supports the concept that a membrane enhances bone defect healing by excluding nonosseous tissues from a defect and providing structural scaffolding for periosteal and endosteal bone regeneration.

Absorption↗
PubMed1995

[Whole organ plastination in otorhinolaryngology].

Total organ sections have proven to be useful in the evaluation of the clinical growth behavior of malignant tumors. However, histologic techniques using tissues embedded in celloidin or paraffin are difficult to produce and require extensive amounts of time. Total organ plastination is a method superior to the aforementioned techniques. The number of artefacts is also greatly reduced by avoiding the need for decalcification. After removal of fat and dehydration, specimens can be placed in epoxy resin. Following evacuation and hardening of specimens, parallel sections can be separated with a diamond wire saw for precision slicing, with section thicknesses ranging from several centimeters to as small as 40 microns. The loss in material caused by slicing is as little as 0.3 mm per section. A further benefit of this method is the relatively large amount of time saved. The entire procedure yielding complete plastinated specimens requires a time period of approximately 40 days. The plastinated specimens are transparent, odorless and fixed and are ideal for instructional and research purposes. Deplastination for the purpose of further diagnositic study is also achievable with a minimum of technical effort.

Epoxy Resins↗
PubMed2003

Effects of 2.94 microm Er:YAG laser radiation on root surfaces treated in situ: a histological study.

BACKGROUND: Previous scanning electron microscopy (SEM) studies using extracted teeth have shown the potential of infrared Er:YAG laser radiation to remove subgingival calculus without causing severe thermal changes, e.g., charring or fusion, to the irradiated root surface. The purpose of the present study was to examine the morphologic changes on root surfaces following Er:YAG laser irradiation in situ using histological observation. METHODS: The periodontal pockets of 6 premolars, canines, and incisors that remained in situ in the jaws of human corpses were irradiated with Er:YAG laser radiation at 60 mJ, 100 mJ, or 180 mJ. The pockets were treated in a similar manner to normal clinical circumstances with a total amount of either 50 or 100 laser pulses. Following laser treatment, the entire tooth, marginal gingiva, and underlying alveolar bone were removed from the jaw. The sections were embedded in methyl-methacrylate, serially cross-sectioned, stained with hematoxylin and eosin or gallamine blue, and examined under a light microscope. Additionally, the extension of the thermally changed tissue areas was determined using digital images and histometry. RESULTS: The histological examination revealed two kinds of thermal changes within the laser-treated root surface. Firstly, a thin superficial layer 5 to 10 microm in width was observed. The surface of this layer showed ultrastructural irregularities. Secondly, a semicircular more deeply stained area close to the apical end of the scaling track beneath the irradiated cementum was observed. The depth of this area ranged from 255 microm to 611 microm and appeared to be independent of the radiation energy. CONCLUSION: In contrast to previous SEM studies, the histological examination indicated thermal changes within the hard tissue bordering the periodontal pocket following Er:YAG laser irradiation.

Aluminum Silicates↗
PubMed1995

Glycans of the early human yolk sac.

The pattern of glycan distribution in the early human yolk sac has been investigated using a panel of lectins. Two 6-week and one 8-week human yolk sacs, and one 8-week fetal liver from live, ectopic pregnancies were fixed and embedded in epoxy resin. Lectin histochemistry was carried out on sections of these tissues using 23 biotinylated lectins and an avidin-biotin peroxidase revealing system. Mesothelial surfaces expressed most subsets of N-glycans (other than high mannose types), N-acetyl-lactosamine, sialic acid, and alpha 1,6-N-acetylgalactosamine. Endodermal surface and lateral membranes resembled those of mesothelium, but showed a preponderance of alpha 2,6-sialyl residues. Most intracellular granules contained N-glycan. There was a marked heterogeneity of granules in the endodermal cells, with different subsets varying in both staining and positional characteristics. The mesenchymal matrix bound most of the lectins used in the study, and expressed fucosyl residues which were also detected in the endothelium. Fetal liver parenchyma showed very similar staining patterns to those seen in the endoderm except for the distribution of N-acetylglucosamine, which was sparse. Despite some common features, each germ cell layer had a distinct 'glycotype', with some saccharides showing extreme topographical restriction.

Biotin↗
PubMed2005

Types of vascular wall as related to vasa vasorum in common tree shrew (Tupaia glis).

Blood vessels of the common tree shrew (Tupaia glis) were embedded in Araldite, sectioned at 0.5-1 microm thickness, and observed with light microscope (LM). It was found that the vascular wall could be classified into three categories: (1) those with proper vasa vasorum (PVV); (2) those with collaborative vasa vasorum in perimural tissues; (3) those without vasa vasorum. The PVV were located in the the tunica media, between the tunica media and the tunica adventitia, and in the tunica adventitia of the vascular wall. On studying the vascular casts with a scanning electron microscope (SEM), it was noted that what appeared to be vasa vasorum did not always conform to those found under the LM. Furthermore, the PVV seen in a particular blood vessel among different tree shrews were not always related to intraluminal partial pressure of oxygen, vascular size, and thickness of the wall. In contrast, the results of this study indicate that the activeness of the vascular wall in varying the amount of blood flow to certain organs is an important factor that is associated with the existence, density, and distribution of the vasa vasorum.

Animals↗
PubMed1999

Increased yield of immunogold labeling of epoxy sections by adding para-phenylendiamine in the tissue processing.

The purpose of this study was to examine if the presence of para-phenylendiamine (PPD) in the tissue processing could increase the yield of immunogold labeling of the epoxy sections. Renal swine tissue with glomerular immune complex deposits with reactivity against IgG was embedded in epoxy resin. PPD was added (1) at the beginning of the dehydration, (2) in the first step with propylene oxide, (3) in the beginning of the dehydration and in all steps with propylene oxide included the infiltration step where propylene oxide and epoxy resin are mixed, or (4) PPD was totally avoided. The tissue was embedded with two different combinations of accelerator. Immunogold labeling with anti-IgG was performed on both non-heated and heated ultrathin sections. The immunogold labeling on the heated sections which were based on processing with PPD in all steps (3) was about 55-65% higher than the corresponding labeling for epoxy sections processed in total absence of PPD (4). The immunolabeling was not significantly increased when the tissue was processed with PPD only in the start of the dehydration (1) or in the first step with propylene oxide (2). We believe that tissue processing with sufficient PPD contributes to reduce the co-polymerization between the antigens and the epoxy polymer in the same way as excess of accelerator does (Brorson and Skjørten, 1996a). The practical significance of this study provides better opportunities for increasing the immunogold labeling of epoxy sections by adding PPD in the tissue processing, and our result may inspire other researchers to develop even more efficient methods for controlling the copolymerization between antigens and epoxy resin.

Animals↗
PubMedDate not supplied

Bone and soft tissue integration to titanium implants with different surface topography: an experimental study in the dog.

The aim of the present experiment was to study the peri-implant soft and hard tissues formed at titanium implants with 2 different surface configurations and to give a topographic description of the surfaces examined. In 5 beagle dogs, the mandibular premolars were extracted. Three months later, 4 self-tapping standard implants (SI) and 4 Osseotite implants (OI) of the 3i Implant System were placed. The marginal 3 mm of the OI is turned, while the remaining part has an acid-etched surface structure. Abutments were connected after 3 months. A plaque control period was initiated, and after 6 months block biopsies were obtained. From each animal 2 units of each implant type were processed and embedded in EPON. The remaining biopsies were processed for ground sectioning. The histometric measurements performed on the EPON sections revealed that the peri-implant soft tissues and the marginal level of bone-to-implant contact were similar for SI and OI sites. In the ground sections, bone-to-implant contact (BIC%) and bone density assessments were made in 2 different zones. Zone I represented the contact area measured from the marginal level of bone-to-implant contact (B) to a position 4 mm above the apex of the implant, and zone II represented the apical 4 mm of the implant. For the SI sites, the BIC% was 56.1% in zone II and 58.1% in zones I + II. The corresponding figures for the 01 sites were 76.7% and 72.0%. The BIC% was significantly larger at OI than at SI sites. Bone density values were similar at the SI and OI sites.

Acid Etching, Dental↗
PubMed1993

Immunogold-silver staining and epipolarized light microscopic detection of phosphoenolpyruvate carboxykinase and glycogen phosphorylase in rat liver.

The subcellular distribution of enzymes related to carbohydrate metabolism was determined in sections of paraformaldehyde fixed and polyethylene glycol-1540-embedded rat liver and in cryostat sections. For this purpose, goat anti-rat phosphoenolpyruvate carboxykinase (PEPCK) serum and rabbit anti-rat glycogen phosphorylase (GP) serum were used as primary antibodies to localize the corresponding antigens. The primary antibodies were localized by 5 nm colloidal gold labeled secondary antibodies (either rabbit anti-goat IgG for PEPCK or goat anti-rabbit IgG for GP), and the gold particles were enhanced by silver staining using appropriate development reagents. The silver enhanced gold particles were detected by epipolarized light microscopy. PEPCK and GP immunoreactive molecules were found only in glycogen-containing areas of the cytosome of hepatocytes, and not in other cells. No immunocytochemical staining of hepatocytes was found when normal serum replaced the primary antibody in the procedures. Visio-Bond semithin (0.35-1.0 micron) sections provided higher resolution for subcellular immunostaining of PEPCK and GP than cryosections of 10 microns. Epipolarized light microscopy provided detection at high sensitivity of the gold-labeled antibody, and combined with transmitted light, allowed simultaneous visualization of the tissue morphology.

Animals↗
PubMed2005

Tissue-plastinated vs. celloidin-embedded large serial sections in video, analog and digital photographic on-screen reproduction: a preliminary step to exact virtual 3D modelling, exemplified in the normal midface and cleft-lip and palate.

This study analyses tissue-plastinated vs. celloidin-embedded large serial sections, their inherent artefacts and aptitude with common video, analog or digital photographic on-screen reproduction. Subsequent virtual 3D microanatomical reconstruction will increase our knowledge of normal and pathological microanatomy for cleft-lip-palate (clp) reconstructive surgery. Of 18 fetal (six clp, 12 control) specimens, six randomized specimens (two clp) were BiodurE12-plastinated, sawn, burnished 90 microm thick transversely (five) or frontally (one), stained with azureII/methylene blue, and counterstained with basic-fuchsin (TP-AMF). Twelve remaining specimens (four clp) were celloidin-embedded, microtome-sectioned 75 microm thick transversely (ten) or frontally (two), and stained with haematoxylin-eosin (CE-HE). Computed-planimetry gauged artefacts, structure differentiation was compared with light microscopy on video, analog and digital photography. Total artefact was 0.9% (TP-AMF) and 2.1% (CE-HE); TP-AMF showed higher colour contrast, gamut and luminance, and CE-HE more red contrast, saturation and hue (P < 0.4). All (100%) structures of interest were light microscopically discerned, 83% on video, 76% on analog photography and 98% in digital photography. Computed image analysis assessed the greatest colour contrast, gamut, luminance and saturation on video; the most detailed, colour-balanced and sharpest images were obtained with digital photography (P < 0.02). TP-AMF retained spatial oversight, covered the entire area of interest and should be combined in different specimens with CE-HE which enables more refined muscle fibre reproduction. Digital photography is preferred for on-screen analysis.

Artifacts↗
PubMed1999

A computerized analysis of the relation between the occlusal enamel caries lesion and the demineralized dentin.

It has previously been shown that the demineralized dentin does not spread laterally along the enamel-dentin junction in non-cavitated approximal lesions. This study examined the spread of the dentinal part of occlusal lesions along the enamel-dentin junction. The material consisted of 48 well-defined occlusal lesions with histological evidence of dentin demineralization. The teeth were embedded in methylmethacrylate, serially-sectioned, ground, and contrast-stained. The sections with the deepest lesion penetration from each lesion were photographed and scanned by a high-quality scanner in order to produce images for computerized histomorphometric analysis. Two variables were measured on the images: i) the length of the contact area of the enamel lesion along the enamel-dentin junction; and ii) the length of the contact area of the demineralized part of the dentin lesion along the enamel-dentin junction. The results showed a gradual increase in the lengths of the enamel and dentin contact areas between the sections, each representing well-defined progressive stages of lesion formation. In two-thirds of the non-cavitated lesions, the enamel lesion was larger than the demineralized part of the dentin lesion, and in 50% of the cavitated cases the extension of the demineralized dentin was less than the affected enamel. Our conclusion is that the dentin demineralization did not spread significantly in a lateral direction in non-cavitated occlusal lesions.

Coloring Agents↗
PubMed2000

Field-emission scanning electron microscopy of resin-dentin interface morphology of seven dentin adhesive systems.

PURPOSE: The purpose of this study was to evaluate the resin-dentin interface morphology of 7 resin-based dentin adhesive systems (Solid Bond, EBS-Multi, PermaQuik, One Coat Bond, Gluma One Bond, Prime & Bond NT/NRC, and Clearfil Liner Bond 2V). MATERIALS AND METHODS: Fourteen dentin disks 1.0 mm thick were obtained from superficial occlusal dentin of extracted human third molars, and finished with wet 600-grit silicon carbide paper. Two dentin disks were bonded using each of the adhesives above according to the manufacturers' instructions, and a thin layer of flowable resin composite was applied. The specimens were kept in tap water for 24 h at 37 degrees C, and then assigned to one of two observational techniques: a fracture technique and an acid-base technique. Fracture technique: shallow grooves were cut, fixed in 10% buffered formalin, and dehydrated in an ascending ethanol series up to 100%, critical-point dried, and fractured along the prepared grooves. Acid-base technique: the specimens were embedded in epoxy resin, sectioned through the center, polished with diamond paste down to 0.25-micron particle size, and treated with 10% orthophosphoric acid for 10 s and 5% sodium hypochlorite for 5 min. All the specimens were mounted on aluminum stubs, gold sputter coated, and observed using field-emission scanning electron microscopy (FE-SEM). RESULTS: All the dentin adhesive systems showed hybrid layer formation, but the thickness varied depending on the bonding system used. The self-etching priming systems (Prime & Bond NT/NRC and Clearfil Liner Bond 2V) showed the thinnest hybrid layer at 1 to 2 microns, whereas the "single-bottle" system (Gluma One Bond) exhibited the thickest hybrid layer at 8 to 16 microns. CONCLUSION: The ultramorphological structures of dentin bonding systems are determined by the composition of each system. Characterization of the interface of the adhesive system using the fracture technique provides additional information regarding the pattern of resin infiltration in some dentin bonding systems.

Adhesives↗
PubMed1996

Ultrastructural localization of B-50/growth-associated protein-43 to anterogradely transported synaptophysin-positive and calcitonin gene-related peptide-negative vesicles in the regenerating rat sciatic nerve.

The growth-associated protein-43/B-50 (B-50/GAP-43) is conveyed from the neuronal soma into the axon by fast axonal transport and moved to the nerve terminal. To visualize and determine the type of vesicles by which B-50/GAP-43 is anterogradely transported in the regenerating rat sciatic nerve, we have investigated Lowicryl HM20 embedded nerve pieces dissected from the proximal side of a collection ligature. Ultrastructurally, numerous vesicular profiles of various sizes, tubules and mitochondria were seen to accumulate proximal to the collection ligature. Both, in unmyelinated and myelinated axons, B-50/GAP-43 immunoreactivity was associated with vesicular profiles which had a diameter of 50 nm. A fraction of the B-50/GAP-43 label co-localized with the small vesicle marker synaptophysin. Co-localization of B-50/GAP-43 was not detected with the large dense-core vesicle marker calcitonin gene-related peptide. These results indicate that, in rat sciatic nerve axons, B-50/GAP-43 is anterogradely transported in small 50 nm vesicles of the constitutive pathway. These transport vesicles were distinguished in two types. We suggest that one type carrying, both, B-50 GAP-43 and synaptophysin has as destination the nerve terminal, whereas the second type, which only contains B-50/GAP-43 and no synaptophysin, may be primarily targeted to the axolemma for local membrane fusion.

Animals↗
PubMed2001

Determination of residual dentine caries after conventional mechanical and chemomechanical caries removal with Carisolv.

The aim of the study was to assess the efficacy of removing dentine caries chemomechanically with Carisolv and with round burs. In 24 extracted permanent teeth each, caries removal with Carisolv or rotating round burs was monitored by checking the hardness of the dentine with a dental explorer and stopped at 12 teeth in each group when either (a) a leather-hard texture was reached or (b) a sharp scratching sound was heard. After embedding and sectioning (400 microm), caries activity of the remaining dentine was assessed using methyl red dye. In microscope images (7x) of the samples, the mean depth of the pink (caries-active, pH<5.5) and yellow (pH>5.5) staining of each carious lesion was calculated. Using a round bur, the depth of caries-active and -inactive lesions was significantly greater when a leather-hard texture (51-58 microm) was reached than when a sharp scratching sound was reached (19-25 microm, t-test, P<0.05). For teeth treated with Carisolv, only the depth of the caries-inactive dentine differed significantly. Regardless of the clinical criterion for caries removal, Carisolv treatment resulted in higher mean depths of caries-active dentine (71-78 microm) than conventional caries removal using the round bur (19-51 microm), while the mean depths of the caries-inactive dentine differed minimally. Thus, caries removal with Carisolv leaves up to a mean of 50 microm more carious dentine than round burs.

Azo Compounds↗
PubMed1998

Comparison of the immunogold labeling of single light chains and whole immunoglobulins with anti-kappa on LR-white and epoxy sections.

The purpose of this study was to examine the intensity of the immunogold labeling of kappa light chains as single molecules and as parts of whole immunoglobulin molecules in LR-White sections and epoxy sections both practically and theoretically. Human renal tissues including deposits of kappa light chains and immune complex deposits of IgA were embedded in both LR-White resin and epoxy resin. Immunogold labeling was performed on unetched thin sections of both resins with anti-kappa or anti-IgA. In all relevant cases the immunolabeling was intense on the LR-White sections. Single kappa light chains were intensely labeled also on the epoxy sections, although not as intensely as on LR-White sections. In contrast, the immunogold labeling of whole immunoglobulins with anti-kapp and anti-IgA was weak and hardly positive on the epoxy sections. Consequently, the quotient labelinglr-white/labelingepoxy for anti-kappa was significantly lower for labeling of single light chains (3.6) than for labeling of whole immunoglobulins (15.9). The corresponding quotient for labeling of whole immunoglobulins with anti-IgA was 17.0. Supported by theoretical considerations, it is believed that the copolymerization between the epoxy resin and the antigens allows the knife to cleave the large whole immunoglobulins more easily than the smaller single kappa chains. This prevents satisfactory immunolabeling of whole immunoglobulins on epoxy sections whether anti-kappa or anti-IgA is used as antibodies, while single kappa chains are easily immunolabeled.

Acrylic Resins↗
PubMed1993

Distribution of noncollagenous proteins in the matrix of adult human bone: evidence of anatomic and functional heterogeneity.

The microanatomic distribution of several noncollagenous proteins (NCPs) in bone matrix was examined by immunohistochemical analysis of glycol-methyl methacrylate-embedded normal adult human bone biopsies. Osteopontin and bone sialoprotein stained throughout the lamellae of both trabecular and cortical bone. Cement lines (cortical and trabecular) and the mineralized matrix immediately adjacent to each Haversian canal were intensely stained. Osteocalcin was detected in cement lines; however, lamellar staining varied depending on the location within the individual unit of bone. In cortical bone, the inner concentric lamellae of osteons were often unstained but the outer lamellae were heavily stained for osteocalcin. Osteonectin was not detected in cement lines and in most specimens revealed a pattern similar to that of osteocalcin with respect to the absence of immunostaining within the inner concentric lamellae. Decorin was prominent in the perilacunar matrix, the canaliculi of osteocytes, and the matrix immediately adjacent to quiescent Haversian canals. Biglycan appeared evenly distributed throughout cortical and trabecular bone matrix. These results suggest that the incorporation of NCPs into matrix may vary depending on the stage of formation of individual bone units. The specific distribution and spatial relationship of these NCPs may be related to the function of each protein during bone resorption and formation. The distinct patterns of NCP localization in bone support the hypothesis that in addition to their structural and mineral-inducing properties, these proteins may influence the events associated with bone remodeling, such as recruitment, attachment, differentiation, and activity of bone cells.

Adult↗