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Abnormalities of nerve conduction and somatosensory evoked potential in Poland's syndrome.

We report a case of Poland's syndrome with associated primary generalized tonic-clonic epilepsy. Somatosensory evoked potentials at median nerve stimulation showed delayed latencies and reduced amplitudes of the evoked response from the hypoplastic right upper limb. Nerve conduction studies showed reduced mixed nerve conduction velocity in the right ulnar nerve and decrease in amplitudes of the compound nerve action potentials in the right median and ulnar nerves. We postulate a congenital hypoplasea of the above nerves and brachial plexus in the present case.

Adolescent↗

Prevalence of Cryptosporidium in sheep and goats bred on five farms in west-central region of Poland.

Faecal specimens were taken from 205 sheep and goats housed in five different localities in the west-central part of Poland. All faecal specimens were examined for Cryptosporidium by using microscopy screening of smears stained by modified Ziehl-Neelsen technique and commercial enzyme immunoassay. PCR technique using genus specific primers was additionally applied in the surveys of 10 faecal specimens collected from lambs. C. parvum infection was identified in 16 of 159 sheep (10.1%). Lambs were more often infected than adult sheep, and the intensity of infection was higher in lambs than in sheep, as a rule. Both lambs and sheep examined in the study were asymptomatically infected with Cryptosporidium. Both microscopy and enzyme immunoassay methods gave one false negative result. The examination of 10 faecal samples revealed 100% agreement among the results obtained by microscopic, immunologic and molecular methods. None of the goats raised on three farms were infected with Cryptosporidium.

Animals↗

A molecular epidemiology study in women from Upper Silesia, Poland.

The present report is a follow-up to our previous molecular epidemiology studies on DNA damage in residents of the industrial region of Upper Silesia. The study was designed to focus on environmental exposure to airborne pollutants; other exposures or confounding factors (e.g. smoking status and age) were eliminated. A Silesian population consisting of 67 donors was compared to 72 inhabitants of a less polluted but similarly urbanized area, surrounded by a rural part of Poland. In both regions the donors were non-smoking females with similar age range, and occupation. Eight biomarkers including urinary mutagenicity and 1-hydroxypyrene, polycylic aromatic hydrocarbon PAH-DNA adducts in oral mucosa, sister chromatid exchanges (SCE), high frequency cells (HFC), chromosomal aberrations (CA), and sensitivity to bleomycin in lymphocytes as well as glutathione s-transferase (GSTM1)/cytochrome P4501A1 (CYP1A1) genotypes were evaluated in samples collected in summer and winter seasons. All the biomarkers of internal and biological doses of mutagens and their early biologic effects indicated statistically significant increases in the Silesian group when compared to the controls. Immunohistochemical quantitation of PAH-DNA adducts additionally revealed significant seasonal changes in the levels of adducts. No influence of susceptibility genotypes (GSTM1 and CYP1A1) on biomarker levels was observed.

Adult↗

STR data for AmpF/STR Profiler Plus loci in south Poland.

Frequency data of short tandem repeats (STR) loci included in the AmpF/STR Profiler Plus kit were collected from a sample of 253 random, unrelated individuals born in the south Poland region. All loci met Hardy-Weinberg expectation.

Alleles↗

Population data of nine Y-chromosomal STR loci in northern Poland.

Allele and haplotype frequencies of nine Y-chromosome STR loci were determined from a sample of 508 unrelated males from north Poland. Three samples containing insertion polymorphism DYS19 (duplication) and DYS385I/II (triplications) were observed.

Chromosomes, Human, Y↗

Antibiotic resistance in Salmonella enterica subsp. enterica strains isolated in Poland from 1998 to 1999.

A total of 326 Salmonella enterica subsp. enterica strains representing 29 serotypes, isolated from human stool specimens during 1998-1999 in sanitary-epidemiological units in Poland were tested for antibiotic susceptibility by a standard disk diffusion method. The antibiotics used were ampicillin, cefotaxime, chloramphenicol, tetracycline, streptomycin, gentamicin, kanamycin, nalidixic acid, ciprofloxacin, furazolidone, cotrimoxazole, sulphonamides and trimethoprim. In addition, 201 strains belonging to the five most commonly isolated serotypes (S. Enteritidis, S. Typhimurium, S. Hadar, S. Infantis and S. Virchow) also had minimal inhibitory concentrations (MICs) determined for amoxycillin/clavulanic acid. Selected strains were screened for production of extended spectrum beta-lactamases (ESBLs). There were 49.4% of Salmonella enterica subsp. enterica strains resistant to two or more antibiotics, with the highest prevalence of multiple resistant strains among serotypes Typhimurium, Hadar and Virchow. Resistance to ampicillin, streptomycin, tetracycline, nalidixic acid, furazolidone and sulphonamides occurred most frequently. Over 93% of S. Virchow strains were resistant to furazolidone. No strains resistant to ciprofloxacin by disk-diffusion method were detected but 31.3% of isolates of the 201 strains representing the five most common serotypes had reduced ciprofloxacin susceptibility (MICs ranging 0.125-0.5 mg/l). One strain (S. Mbandaka) was resistant to cefotaxime and produced ESBL.

Anti-Bacterial Agents↗

Primary resistance to clarithromycin in clinical strains of Helicobacter pylori isolated from children in Poland.

Helicobacter pylori resistance to clarithromycin is an important factor in the failure of eradication therapy. The resistance results from point mutations in the 23S rRNA gene of H. pylori. The prevalence of primary resistance of H. pylori to clarithromycin in children and mutations associated with resistance were studied and it was found that 23.5% (23/98) of H. pylori strains isolated in our hospital during 1998-2000 were resistant to clarithromycin. The primary resistance was mainly caused by an A2143G mutation, but the isolates with an A2142G mutation had higher MICs for clarithromycin compared with those with an A2143G mutation: median MIC 256 versus 16 mg/l. Comparison of our data with previous results showed that the prevalence of H. pylori resistance to clarithromycin in children has increased in Poland over the last three years, however the difference was not significant (23.5 vs. 17%, P=0.22).

Adolescent↗

The etiological agent of Lyme disease, Borrelia burgdorferi, in ticks (Acari:Ixodidae) from eastern Poland.

In 1994, 4204 out of 4656 Ixodes ricinus collected both from vegetation in three different areas and from hunter-killed deer and wild boar in the Białystok province (eastern Poland) were examined individually for the presence of Borrelia burgdorferi s.I. Detection of spirochetes was carried out by the routine indirect immunofluorescence assay (IFA) using polyclonal antibody (anti-B. burgdorferi, strain 1 B 29). B. burgdorferi spirochetes were evident in 349 (8.8%) nymphal and adult I. ricinus collected from vegetation (n = 3958) and in 8 (3.2%) of those removed from hosts (n = 246). Among the ticks collected from vegetation, infection rates in nymphs (5.8-6.4%) in particular areas were about 2-4 times lower than in adults (8.1-24.6%). The calculated minimal and maximal infection rates of ticks collected from different sites were 1.6% and 15.4%, respectively. Prevalence of B. burgdorferi in I. ricinus was determined with respect to the abundance and seasonal activity of the ticks.

Animals↗

Evaluation of the effectiveness of vaccination against diphtheria among medical staff of the chair and the Jagiellonian University Hospital of Infectious Diseases in Cracow, Poland. Some epidemiological observations.

In Poland, twenty-five cases of diphtheria, mostly among adults (including five foreigners) in the region adjacent to the eastern border were registered in the years 1992-1996. In 1993-1994, because of the threat of diphtheria infection, 41 healthy members of the medical staff of the University Hospital of Infectious Diseases in Kraków, mainly women aged 25-57 years were immunized with Td and d vaccines with a 6-month interval between courses. Prior to vaccination, 17(41%) individuals showed a lack of diphtheria antibodies as determined with a passive haemagglutination test. 14 of them were aged 30-49 years. Six months after the 2nd dose of vaccine, all seronegative persons had developed a protective level of antibodies. Moreover, all vaccinated subjects showed a statistically significant increase of the antibody titre between the first and last serological examinations.

Adult↗

Plutonium and other alpha-emitters in bones of wild, herbivorous animals from north-eastern Poland.

The results of the investigation of (239+240)Pu, 241Am and thorium isotope activity concentrations in bones of wild herbivorous animals (deer, roe-deer, boars, elks) from north-eastern Poland are presented. The area on which the animals were living had relatively high Chernobyl plutonium fallout. Possible traces of plutonium were seen only in four samples, but only for one it was it 3sigma above background, at 6.2 +/- 2.4 mBq/kg (ash). The 241Am activity concentration was below the detection limits in all samples. The maximum 228Th concentration in the samples was 7.81 +/- 0.44 Bq/kg (ash), calculated under the assumption of 100% thorium recovery. This isotope seems to originate in bones not from direct incorporation but from the decay of 228Ra. Large variations in activity concentration wose observed, as well as some differences between boars and the chewing animals.

Americium↗

Plutonium and other alpha emitters in mushrooms from Poland, Spain and Ukraine.

The paper presents results on Pu, U and Th isotope activity concentration measurements in some mushroom samples collected in Poland, Spain and Ukraine. The sampling sites differ a lot with regard to observed levels of Pu, its origin and isotope ratios as well as the environmental properties. Some of the Polish samples were collected in the northeastern part of the country with up to 30 Bq/m2 of Chernobyl Pu deposition. Other Polish and the Spanish samples are from areas with almost exclusively global fallout Pu present. Ukrainian samples were collected in a highly contaminated area with a deposition of about 3.7 kBq/m2 of Chernobyl (239-240)Pu. The maximum (239+240)Pu activity concentration was found equal to (54+/-4) Bq/kg (dw--dry weight) for a Ukrainian Cantharellus cibarius sample. Ukrainian samples have an extremely high radiocesium level, with maximum of (51+/-4) MBq/kg (dw). The maximum (239+240)Pu activity concentration for Polish samples was (81+/-5) mBq/kg (dw) for Xerocomus badius. From the isotopic ratio in this sample it can be concluded that Chernobyl fallout is the origin of Pu. More than twice as large was the Spanish maximum for Hebeloma cylindrosporum but with only global fallout Pu. Some aspects of the transfer of nuclides to fruit bodies is discussed and in some cases the transfer factors or aggregation coefficients were calculated. Especially high transfer factors were found for Hebeloma cylindrosporum from Spain.

Agaricales↗