Effect of a twelve week exercise programme on cardiorespiratory and body composition variables in nonobese young and middle-aged females.
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OBJECTIVES: To investigate the effect of different durations of stretching (five or 15 seconds) on active and passive range of motion (ROM) in the lower extremity during a five week flexibility training programme. METHOD: Twenty four university sport club members (19 men, five women), with a mean (SD) age of 20.5 (1.35) years, were randomly assigned to one of three groups (two treatment and one control). The two treatment groups participated in a static active stretching programme three times a week for a five week period, holding each stretch for a duration of either five or 15 seconds. The total amount of time spent in a stretched position was controlled. The five second group performed each stretch nine times and the 15 second group three times resulting in a total stretching time of 45 seconds for both groups for each exercise. The control group did not stretch. Active and passive ROM were determined during left hip flexion, left knee flexion, and left knee extension before and after the training programme using an inclinometer. RESULTS: Two factor within subject analysis of variance indicated no significant difference in ROM before and after the training programme for the control group. However, significant improvements in active and passive ROM (p < 0.05) were shown in both treatment groups after the five week training programme. Two factor analysis of variance with repeated measures and post hoc analysis showed significant differences between the treatment groups and the control group for the improvements observed in active (p < 0.05) and passive (p < 0.05) ROM. The five and 15 second treatment groups did not differ from one another when ROM was assessed passively, but significant differences were apparent for active ROM, with the 15 second group showing significantly greater improvements (p < 0.05) than the five second group. CONCLUSION: These findings suggest that holding stretches for 15 seconds, as opposed to five seconds, may result in greater improvements in active ROM. However, sustaining a stretch may not significantly affect the improvements gained in passive ROM.
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The frequency of diagnosis of congenital scoliosis in the neonatal period is expected to rise given the increasing survival of high risk neonates in the surfactant era and their frequent exposure to x rays. Considering its significant long term implications a neonatologist cannot afford to ignore the diagnosis of congenital scoliosis in a neonate as close surveillance, early detection, and treatment may prevent/minimise the wide spectrum of potentially serious deformities that can affect the developing spine. The review provides general guidelines to help the neonatologists in counselling the parents and in planning the multidisciplinary follow up for management of congenital scoliosis.
This study was done to determine (a) whether in coronary artery disease (CAD) left ventricular (LV) adaptations differed after 6 months of walking/jogging (legs-only, LO) versus aerobic circuit training (arms and legs, AL) versus a control group, and (b) whether a transfer of fitness to the untrained arms in the LO group was related to superior LV adaptations. Peak oxygen uptake for arm and leg ergometry and for cycle ergometry using radionuclide cardiac angiography were performed before and after training. Leg and arm VO2peak increased significantly by 13% in the AL group, and by 13% and 7%, respectively, for the LO group. LV function was greater after training for the LO versus the AL group. Improvements in systolic and diastolic function and a speculated hypervolemia explain these LV adaptations. In CAD patients, walking/jogging produces greater LV function improvements versus circuit training, possibly due to differences in the exercised muscle mass.
The purpose of this study was to assess the reliability and validity of a portable metabolic system (TEEM 100) during submaximal and maximal (VO2max) exercise using a computer-based metabolic system as the reference system (REF). Between repeated trials of submaximal exercise at three constant loads, differences in ventilation (Ve) and oxygen consumption (VO2) were 0.2 +/- 4.9 L . min-1 and 0.03 +/- 0.10 L . min-1 for REF, and 1.9 +/- 0.7 L . min-1 and 0.00 +/- 0.17 L . min-1 for TEEM 100. Pooled intraclass reliability coefficients for Ve and VO2 calculated from the repeated submaximal trials were r = .89 and r = .94 for REF, and r = .86 and r = .94 for the TEEM 100. Respiratory exchange ratio (RER) measured by the TEEM 100 was significantly higher (p = .01) at only the lowest workload. At VO2max, the TEEM 100 recorded significantly higher values for FeO2 (p = .01) and RER (p < .001). These results suggest that the TEEM 100 provides reliable and valid measurements of VO2 during submaximal and maximal exercise.
A comprehensive analysis of the role of the actin cytoskeleton in exocytosis of the four different neutrophil granule subsets had not been performed previously. Immunoblot analysis showed that, compared with plasma membrane, there was less actin associated with secretory vesicles (SV, 75%), gelatinase granules (GG, 40%), specific granules (SG, 10%), and azurophil granules (AG, 5%). Exocytosis of SV, SG, and AG was measured as increased plasma membrane expression of CD35, CD66b, and CD63, respectively, with flow cytometry, and GG exocytosis was measured as gelatinase release with an ELISA. N-formylmethionyl-leucyl-phenylalanine (FMLP) stimulated exocytosis of SV, GG, and SG with an ED(50) of 15, 31, and 28 nM, respectively, with maximal response at 10(-7) M FMLP by 5 min, while no exocytosis of AG was detected. Disruption of the actin cytoskeleton by latrunculin A and cytochalasin D induced a decrease in FMLP-stimulated CD35 expression after an initial increase. Both drugs enhanced the rate and extent of FMLP-stimulated GG, SG, and AG exocytosis, while the EC(50) for FMLP was not altered. We conclude that the actin cytoskeleton controls access of neutrophil granules to the plasma membrane, thereby limiting the rate and extent of exocytosis of all granule subsets. Differential association of actin with the four granule subsets was not associated with graded exocytosis.
Six well-trained endurance athletes were studied to determine if submaximal treadmill exercise results in increased plasma levels of beta-endorphin/beta-lipotropin (Bh-Ep/Bh-LPH) immunoreactivity. Bh-Ep/Bh-LPH immunoreactivity was measured by radio immunoassay in plasma from six experienced runners before and after 30-min treadmill runs at a self-selected pace, 60 and 80% VO2 max, and a control experiment of 30 min rest. All tests were randomized and occurred during the same time of day for a given subject (0600--1500 h). Preexercise Bh-Ep/Bh-LPH values averaged between 10 and 20 pg/ml and increased two- to fivefold after each run. The increase was statistically significant (P less than 0.05) only after the 60% run when Bh-Ep/Bh-LPH increased to a mean of 58.3 pg/ml. A large individual variation in the Bh-Ep/Bh-LPH response to running was noted. Mood state and perceptual data were also collected, and no significant relationship with Bh-Ep/Bh-LPH was evident. These data suggest that the stress of treadmill running acts as a stimulus to greater Bh-Ep/Bh-LPH secretion, a reduction in its degradation, or a combination of these, which leads to increased levels of these ligands in venous blood. The physiological significance of these increased plasma levels is not clear.