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At least 1,009 records · Page 56Linked to original sources
PubMed1968

Studies of base-sequence homology among some cytoplasmic deoxyriboviruses of vertebrate and invertebrate animals.

Relationships among some cytoplasmic deoxyriboviruses were investigated by deoxyribonucleic acid/ribonucleic acid hybridization experiments. Evidence of base-sequence homology between nucleic acids of different viruses was found within, but not between, the poxvirus and iridescent virus groups. Viruses of the vaccinia subgroup of poxviruses were 30 to 100% related, and the degree of relatedness was correlated with serological relatedness. Relationships within the iridescent virus group depended on the laboratory of origin and passage history of the virus strains used. None of these viruses was related to a superficially similar virus of frogs.

DNA, Viral↗
PubMed1981

Chemical methylation of RNA and DNA viral genomes as a probe of in situ structure.

We used [methyl-(3)H] dimethyl sulfate to probe the genome structures of several RNA and DNA viruses. We compared sites of modification in nucleic acids that were methylated chemically before and after extraction from purified virions. With both single-stranded and double-stranded substrates alkylation occurred mainly at the N7 position of guanine. However, adenine N1 atoms were differentially accessible in single-stranded RNA and DNA. For example, the ratios of 1-methyladenosine to 7-methylguanosine for reovirus mRNA and deproteinized genome RNA were 0.43 and 0.03, respectively. Members of the Reoviridae methylated in situ yielded RNAs with ratios of 0.04 to 0.08, indicating that the intravirion genomes were double stranded. We obtained ratios of 0.26 and 0.35 for the RNAs of dimethyl sulfate-treated brome mosaic and avian sarcoma virions, respectively, which was consistent with partial protection of adenine N1 sites by structural proteins or genome conformation or both. The ratios of 1-methyladenosine to 7-methylguanosine for vaccinia virus DNAs methylated in situ (0.10) and after phenol extraction (0.14) were less than the ratios for phiX174 and M13 DNAs (0.39 to 0.64) but considerably greater than the ratio observed with adenovirus DNA (0.002 to 0.02). The presence of a single-stranded region(s) in the vaccinia virus genome was confirmed by S1 nuclease digestion of [methyl-(3)H] DNA; the released radiolabeled fraction had a ratio of 0.41, compared with 0.025 for the residual duplex DNA. In addition to the structure-dependent accessibility of adenine N1, methylation of adenine N3 was severalfold lower in the intravirion genomes of vaccinia virus, phiX174, and adenovirus than in the corresponding extracted DNAs. Chemical methylation of virions and subviral particles should be useful for in situ analyses of specific regions of RNA and DNA genomes, such as the sites of protein binding during virus maturation.

Adenine↗
PubMed2006

[Modeling of Spodoptera litura nuclear polyhedral virus epidemic at different temperatures].

The study on the nuclear polyhedral virus epidemic of Spodoptera litura under effects of temperature showed that at above 29 degrees C, S. litura larvae started to die 2-3 d after virus-inoculation, and reached the peak 4-5 d after the inoculation. The mortality and death velocity of virus-infected host larvae were increased with increasing incubation temperature. No thermo-inhibition temperature for the virus was observed in the test temperature range, while there existed a constant temperature range, i.e., 29-35 degrees C, for the highest mortality of infected host larvae. Complementary log-log regression model could well simulate the daily distribution of host larvae mortality, and the cumulative disease death time could be described by Gompertz model. The revised biophysical model could well simulate the relationship between S. litura larvae mortality and temperature, and be applied to virus epidemic forecasting.

Animals↗
PubMed1985

Gene assignments of a cytoplasmic polyhedrosis virus (type 2) from Nymphalis io.

The 10 double stranded (ds) RNAs from cytoplasmic polyhedrosis virus (type 2) from Nymphalis io have been separated by polyacrylamide gel electrophoresis and fractionated into separate genes. Separate and total dsRNA were denatured using methyl mercury, and translated in a rabbit reticulocyte lysate. Translation of unfractionated RNA generated proteins that comigrated with virion and polyhedral structural proteins and six major non-structural proteins. Translation of individual RNAs, demonstrated that the virion structural proteins were coded for by the largest RNAs and the polyhedral protein by the smallest genome segment. RNAs 7 and 8 each produced two major non-structural proteins and no primary product was found for RNA 3. Reaction of the translation products with antiserum raised against purified virions immunoprecipitated putative structural proteins (and some "non-structural"). Antiserum against the polyhedral protein immunoprecipitated "non-structural" proteins and the polyhedral protein, but not virus particle structural proteins.

Genes, Viral↗
PubMedDate not supplied

[DNA packing inside viral particles of Agrotis segetum (Schiff) turnip moth granulosis virus].

The structure of high molecular (88 million daltons) DNA of turnip moth granulosis virus obtained in the linear and cyclic forms was studied. Electron microscopic examination of the fine structure of DNA isolated from virus particles treated with n-toluene sulphonate-N-cyclohexyl-N'-beta-4 (methyl morpholine)-ethylcarbodiimide were carried out. Modified DNA areas were visualized by two methods: with P32 protein and phosphotungstate acid. In both cases a period of recurrence close to the length of the virus particle was revealed. This study confirmed experimentally the previously suggested model of the existence of structural disorders in DNA caused by bends of superspiral molecule at the ends of the virus particle.

CME-Carbodiimide↗
PubMed1975

[Demonstration, stabilization and purification of an intracapsid nucleoprotein structure of Baculovirus of Oryctes rhinoceros L].

The presence of a structurally organized nucleoproteic structure in the capsid of the Baculovirus of Oryctes rhinoceros L. is shown. This structure is stabilized under definite conditions described in detail in the paper. It possesses a rope-like structure of about 280 nm in length on 15 nm in diameter containing the DNA molecule. A basic protein is found in the virus.

Animals↗