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PubMedDate not supplied

[Fasciola hepatica in the common bile duct. Presentation of a case].

A case of hepatic fascioliasis in the common bile duct is reported in a 54 years female. Upon surgical exploration of the biliary tract, one adult form of hepatic Fasciola was found, without evidence of cholelithiasis. Retrospectively we reviewed the oral cholecystography and the image originally believed to be gallstone, in fact was a hepatic Fasciola. Reviewing past literature, we have not encountered any previous case in which hepatic Fasciola was diagnosed by oral cholecystography.

Common Bile Duct Diseases↗
PubMed1990

Potential for the spread of Fasciola hepatica in cattle in Oklahoma.

In 29 central and western Oklahoma counties, 42 ranches were investigated for indigenous Fasciola hepatica infections and their suitability for the transmission of liver fluke. A 10-year retrospective study of Oklahoma cattle based on samples submitted to the Oklahoma Animal Disease Diagnostic Laboratory also was done. Indigenous fascioliasis was found in cattle in 12 counties (9 central and western counties identified in the combined field and retrospective studies and 3 additional eastern counties identified on the basis of the retrospective study). Factors essential for survival of free-living stages of F hepatica and for snail species necessary for propagation of the fluke existed in much of Oklahoma. Snails capable of serving as intermediate hosts for transmission of the fluke reportedly have been found in 41 of the 77 Oklahoma counties. Lymnaeid snails were detected in 4 counties in the field study.

Animals↗
PubMed1978

The efficiency of diamphenethide in controlling natural infestations of Fasciola hepatica in lambs.

The activity of diamphenethide against natural infestations of Fasciola hepatica in fattening lambs was assessed on a farm with a noted history of fascioliasis. The main experimental groups, two treated and one control, grazed known contaminated pastures for a twelve week period after which they were removed to fluke-free areas to await slaughter. Three groups of four tracer lambs were each grazed for three successive four week periods with the main flock to determine continuity of infection. A single treatment with diamphenethide at 110 mg/kg bodyweight given when the sheep were removed from the trial area was 96.7% effective against the sub-chronic disease which had developed by this time. Three treatments at 110 mg diamphenethide/kg bodeweight given at intervals of four weeks during the grazing period proved to be 100% effective in preventing the establishment of the disease. Significant benefits in terms of weight gain were exhibited by the treated lambs.

Acetanilides↗
PubMedDate not supplied

[Detection of immunoreactive peptides of Fasciola hepatica, with sera from infected subjects, by enzyme immunoelectrotransfer].

In order to observe the immunoreactive peptides in a crude extract of adult Fasciola hepatica specimens, proteins were separated by 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to nitrocellulose paper. Next, sera from 14 human cases of F. hepatica infection, parasitologically confirmed, which presented high titers of specific antibodies against F. hepatica detected by ELISA, were reacted with the blotted peptides and immunodetected by an anti-human IgG conjugated with horse-radish peroxidase. SDS-PAGE showed at least 18 bands ranging 96 to 14 Kd. Groups of peptides weighing 94-66 Kd, 43-36 Kd and 35-14 Kd reacted with serum antibodies of 12 fascioliasis patients. In the remaining two cases, reactive peptides were not clearly observed. The 94-66 Kd components were immunoreactive with 12 out of the 14 serum samples. On the other hand, 43-36 Kd peptides reacted with 4 of the 14 sera and only 3 out of the 14 sera of infected individuals showed reaction with 30-14 Kd. F. hepatica infection induces in humans diverse antibody responses, being 94-66 Kd bands the most immunoreactive peptides and would be potential serodiagnostic antigens.

Adolescent↗
PubMed1989

Detection of circulating immune complexes by the enzyme-linked immunosorbent assay in sera from cattle infected with Fasciola hepatica.

Polyethylene glycol-precipitated immune complexes (PIC) from the sera of 5 calves with Fasciola hepatica worm burdens ranging between 27 and 70 flukes were examined for parasite antigen content at 2, 4, 6, 8, 10, and 16 wk postinfection (PI) by the enzyme-linked immunosorbent assay (ELISA). Three assays were devised using an affinity-processed rabbit antibody to worm excretory/secretory (FhES) antigens. The PIC plate assay detected parasite antigen by adherence of anti-FhES antibody to PIC incubated overnight on ELISA plates, and tests were visualized using anti-rabbit peroxidase-linked antibody. The serum complex and PIC capture assay utilized the anti-FhES immunoglobulin as an antigen capture antibody linked to the solid phase. The attached complexes were then detected by the adhering bovine antibody, either soluble complexes in serum or as PIC. All assays showed circulating immune complex (CIC) values elevated at 6-8 wk PI, which generally coincided with increased host circulating antibody to FhES antigens. The greatest detection rate for all of the immune complex (IC) detection assays occurred with the PIC capture assay. It detected antigen in almost 90% of sera tested at 6 and 8 wk PI. Both the serum complex and PIC capture assay detected greatest amounts of CIC in those animals with the largest worm burdens, whereas the PIC plate assay showed no such trend. This study shows that F. hepatica antigen detection in CIC can be used to aid immunologic diagnosis of fascioliasis.

Animals↗
PubMed1985

Diagnosis of Fasciola hepatica infection in beef calves by plasma enzyme analysis.

Plasma analysis for albumin, total bilirubin, and total protein values and aspartate aminotransferase (AST), arginase, and gamma-glutamyl transpeptidase (GGT) activities was used for the early and quantitative diagnosis of experimental Fasciola hepatica infections in beef calves. Calves were infected on 3 occasions with 1,000 (n = 5), 100 (n = 5), or 10 (n = 4) metacercariae for a total infective dose of 3,000, 300, or 30, respectively. Albendazole (15 mg/kg of body weight) was administered to 7 infected calves on postinfection (initial) week (PIW) 13. All calves were euthanatized and necropsied on PIW 16 for the determination of fluke infections. Plasma constituents were determined weekly. Significant (P less than 0.05) increases in AST activity occurred as early as PIW 4 and GGT activity at PIW 9, as compared with that in noninfected controls. Fluke burden-related differences were observed in GGT activity from PIW 9 onward. Increases in AST activity reflected parenchymal liver damage, whereas increases in GGT reflected hepatobiliary damage; therefore, differentiation could be made between the migratory and ductal phases of the infection. There was no correlation between arginase activity and fluke infection. As compared with fecal examination results, plasma enzyme analysis gave an earlier and semiquantitative indication of F hepatica infection in experimentally infected calves. Although increases in these plasma constituents were not definitely diagnostic of fascioliasis, useful information on the size of the fluke burden and progress of the disease process could be obtained by these methods. Plasma enzyme analyses of AST and GGT were not indicative of chemotherapeutic success or failure when calves with mature F hepatica (14 weeks old) infections were treated.

Animals↗
PubMed1986

[Unusual echographic aspects of hepatic distomiasis].

The authors report, a pediatric case of hepatic fascioliasis with an unusual ultrasonographic aspect; that is a hypoechoic intrahepatic nodule with an extrahepatic prolongation through a thickened capsule. This ultrasonographic appearance, which has not been reported in the literature to our knowledge, seems to reflect the parasite migration.

Child↗
PubMed1988

[Early diagnosis of liver fluke infection in sheep].

The sera and livers of slaughtered lambs were studied in October 1987 in order to achieve a suitable interpretation of the indirect haemagglutination assay (IHA) in the diagnosis of fascioliasis in flocks. In view of these studies, it is concluded that the IHA is useful in the early diagnosis of liver fluke infection in lambs. It is recommended to take samples from at least five lambs in each flock. An interpretation model is provided.

Animals↗
PubMed1988

Fasciola in horses in the Republic of South Africa: a single natural case of Fasciola hepatica and the failure to infest ten horses either with F. hepatica or Fasciola gigantica.

The faeces of 11 horses were examined for Fasciola spp. eggs. One of them was positive for Fasciola hepatica, a finding which was confirmed post-mortem. The 10 negative horses were subsequently infested with either F. hepatica or Fasciola gigantica, each animal receiving orally from 500-9,500 metacercariae. No clinical signs were observed and no fluke eggs were detected in the faeces, and neither immature nor adult Fasciola worms were recovered from the horses slaughtered 16-26 weeks post-infestation with F. hepatica and 28-34 weeks post-infestation with F. gigantica, respectively. The results indicate that horses have a high level of resistance to both South African Fasciola spp. Factors which can probably explain the conflicting reports for the prevalences of fascioliasis in horses throughout the world and the varying results obtained in experimental infestations are briefly considered.

Animals↗
PubMed1977

Antibody response of calves to a single infection of Fasciola gigantica determined by an indirect fluorescent antibody technique.

The antibody response of six calves infected with 500 metacercariae of Fasciola gigantica each was monitored throughout 30 weeks of infection using an indirect fluorescent antibody technique (IFA). In vitro excysted F gigantica were employed as the test antigen. All animals showed high antibody titres from two to six weeks post-infection. Thereafter the antibody titres diminished gradually. Although all the experimentally infected animals harboured fluke burdens at autopsy, most gave negative IFA tests from 22 weeks post infection onwards. Specific immunofluorescent staining occurred on the surface glycocalyx of the newly excysted flukes. It is likely that this glycocalyx provides one of the earliest antigenic stimuli for the host's immune reactions to fascioliasis.

Animals↗
PubMedDate not supplied

[Current aspects of digestive actinomycoses. Apropos of a case of hepatic actinomycosis].

A case of hepatic actinomycosis is described in a patient with fascioliasis with Fasciola hepatica. The authors emphasize the abdominal sites of actinomycosis, specially appendix-cecum. Diagnosis, often difficult, is evoked before fistula with yellow granules in the pus. Definitive identification requires anaerobic culture. The treatment with lengthy antibiotic administration is efficient.

Actinomycosis↗
PubMed1988

[Fasciola hepatica infection. Successful therapy using triclabendazole].

We report a case of human fascioliasis treated successfully with a single dose of triclabendazole, a benzimidazole compound. The most obvious effect was the rapid cessation of faecal egg excretion, which had been refractory to two previous treatment courses of albendazole. Cure seems to be confirmed by the absence of Fasciola eggs in repeated stool examinations after six months, achievement of clinical wellbeing, and normalization of laboratory tests. The only side effect of the treatment was a brief episode of fever and right upper abdominal pain occurring four days after administration of triclabendazole. The syndrome was probably due to disintegrating dead parasites; further observations are needed to explain the pathogenesis of this episode.

Animals↗
PubMed1987

Herd evaluation of Fasciola hepatica infection levels in Louisiana cattle by an enzyme-linked immunosorbent assay.

An enzyme-linked immunosorbent assay (ELISA) was evaluated as a method of determining relative immunoconversion rates in calves and how immunoconversion rates and strength of optical density values correlate with prevalence of Fasciola hepatica fecal egg shedding. Ten to 55 calves and cows were examined from each of 10 separate beef cattle herds in central and southern Louisiana. Infection prevalence rates for calves averaged 8% higher when ELISA optical density values were used than those when fecal egg count data were used. Of 55 calves in 8 herds that were ELISA positive, 39 were shedding F hepatica eggs; of 53 calves that were shedding eggs, 14 were ELISA negative. Significant correlation of calf fecal and ELISA prevalence was observed for 8 herds by linear regression analysis. A chi 2 analysis showed that calf ELISA and fecal egg shedding data were not independent. Results indicate that positive ELISA reactions for as few as 10 to 15 calves from the fall calf crop of a given herd are sufficiently accurate to be used to assess F hepatica herd infection rates, the likelihood of liver condemnations at feedlot destinations, and variation between individual farms in fascioliasis infection risk. The test was less valuable as a diagnostic test when used in adult animals previously exposed to F hepatica or on an individual animal basis.

Animals↗
PubMed1985

[IgE antibodies in human Fasciola hepatica distomiasis].

In patients infected by Fasciola hepatica, total IgE and specific IgE antibodies have been determined by radioimmunoassays, and IgG, IgA, IgM levels by radial immunodiffusion test (Mancini, 1965). Moreover, total and specific IgE levels have been related to parasite egg burden, age, clinical features and eosinophilia. Elevated total IgE and specific IgE antibodies levels have been found respectively in 76% and 48% of the patients whereas there was no significant variations in other immunoglobulins levels. However, though the amount of total and specific IgE was lower than in other helminthic diseases, it appears to be a significant data of the immune response to parasites as it has been reported and discussed previously. It has been shown a significant relationship between total and specific IgE levels, the number of lines by immunoelectrophoresis, and the results of the indirect haemagglutination and indirect fluorescent antibody techniques; each method appeared to be in equal value to perform the early diagnosis of human Fasciola hepatica. In addition, specific IgE antibodies levels were correlated with eosinophilia specially when it exceeds 15%. This results demonstrate the availability of their measurement in the diagnosis of fascioliasis versus other diseases with marked eosinophilia.

Eosinophilia↗
PubMed1985

[Fasciolar hepatic abscesses: value of hepatic ultrasonography. Apropos of 3 cases].

Hepatic abscess is a rare complication of fasciolasis. The ultrasonographic appearances of these abscesses are not well known. The finding of nodular echodense images in the hepatic parenchyma may cause diagnostic confusion. We report three cases in which abdominal ultrasonography demonstrated hepatic abscesses complicating fascioliasis. Follow-up examination showed regression of these lesions after treatment.

Adult↗
PubMed1985

Experimental cholecystitis in goats caused by mature Fasciola gigantica.

Mature Fasciola gigantica obtained from naturally infected cattle were surgically transferred into the gallbladders of six fluke-free goats. Two of the goats died at two and 19 weeks and the remaining four were slaughtered at 12, 48, 48 and 80 weeks after the transfer. Nine of 20 transferred F gigantica were recovered from the gallbladder and common hepatic duct of the experimental goats. The walls of gallbladders and bile ducts were thickened by epithelial proliferation, fibrosis and mononuclear cell infiltration. Large numbers of globule leucocytes were present in the epithelium of gallbladders and common hepatic ducts as well as in minute intrahepatic bile ducts. Very few mast cells were observed in these locations. Globule leucocytes in two goats were positive for argentaffin reaction with Gomori hexamine silver stain. The proliferative cholecystitis in experimental goats was similar to adenomatous cholecystitis in cattle naturally infected with fascioliasis. Globule leucocytes had no obvious deleterious effects on the parasites.

Animals↗
PubMed1985

[Epidemiologic data on distomiasis due to Fasciola gigantica Cobbold on the island of Madagascar. Infestation of cattle between 1975 and 1981].

Stools examinations of zebus were made in Madagascar between 1975 and 1981 for determining the importance of Fascioliasis gigantica in the six provinces of the island during this time. High percentages of F. gigantica eggs were found in the stools of the different infected zebus-herds of five provinces. No fasciolid infection was noted in 1979 in the cattle of the province of Diego-Suarez. Differences were noted between the infection rate obtained by fecal examination and the rate indicated in the veterinary reports. The significance of these results is discussed.

Animals↗
PubMed1980

Dynamics of Lymnaea natalensis populations in the Zaria area (Nigeria) and the relation to Fasciola gigantica infections.

Observations on the seasonal changes in the Lymnaea natalensis population and the infection rate of these snails with Fasciola gigantica during 1972-1976 are reported. The data were obtained from snails in three different locations near Zaria in Northern Nigeria. One of the survey sites dried out during the drought of 1972-1974 and had to be abandoned. The snail population showed seasonal variations with the lowest number of snails at the very end of the dry season. Many juvenile snails developed at the end of the wet season and beginning of the dry season, and the population reached its climax at the middle of the dry season. Fasciola infections in the snails occurred throughout the year, but the majority of the infections appeared to be acquired during the end of the wet season resulting in a high infection rate (up to 25%) in the beginning of the dry season. There was evidence of accelerated growth and gigantism in the infested snails. The occurrence of Fasciola infections in snails appears to depend on the presence (or absence) of livestock in snail infected areas during the rainy season. The infection developing in the rainy-season generation of snails is of major importance in the dissemination of fascioliasis in livestock as metacercarial shedding more or less coincides with the river-valley grazing of cattle.

Animals↗