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Results for “DECONTAMINATION”

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At least 1,009 records · Page 56Linked to original sources
PubMed1982

Methods of decontamination of anaesthetic equipment: daily sessional exchange of circuits.

Sampling of untreated anaesthetic circuits showed heavy bacterial contamination. Daily or twice daily sessional exchange of circuits (i.e. use of one circuit for up to nine patients) considerably reduced the extent of contamination. Equipment was effectively treated by steam at high or low temperature or by immersion in glutaraldehyde. Steam at high temperature (134 degrees C) damaged reservoir bags on repeated treatment, whereas low temperature steam (68 degrees C) was satisfactory. Treatment with glutaraldehyde was also satisfactory, but slightly less reliable and its smell was difficult to remove from the equipment after repeated immersion.

Anesthesiology↗
PubMed1999

Decontamination of human sclera: an in vitro study.

PURPOSE: The human sclera is frequently used in ophthalmic surgeries and must be preserved in disinfectants that prevent its contamination. In this study the efficiency of glycerin, absolute alcohol (ethanol), and benzalkonium chloride (1:5,000) as human sclera disinfectants were compared. METHODS: Fresh human scleras were trephined, the scleral disks divided into three groups and contaminated with Staphylococcus aureus (ATCC 29213), Pseudomonas aeruginosa (ATCC 27853), or Bacillus cereus (ATCC 11778) for 24 h. Thereafter they were transferred to preservation vials each containing glycerin, absolute alcohol, benzalkonium chloride diluted in 70% alcohol (1:5,000) or Trypticase Soy Broth (control), respectively, and stored at room temperature. From each vial, two scleral disks were removed after 1, 2, 3, 4, 7, 10, and 14 days of immersion. Both were plated on blood agar, one being macerated, and both incubated at 37 degrees C for 48 h. RESULTS: Pseudomonas aeruginosa, S. aureus, and B. cereus were recovered from the glycerin-immersed scleral disks until the second, fourth, and fourteenth days, respectively. Bacillus cereus was recovered from those immersed in absolute alcohol until the fourteenth day, whereas disks infected with the other microorganisms and immersed in absolute alcohol presented no growth since the very first day of immersion. Bacillus cereus was recovered from scleral disks immersed in benzalkonium chloride diluted in 70% alcohol (1:5,000) only on the first day. CONCLUSION: Resistant microorganisms can survive in scleral tissue preserved in glycerin and absolute alcohol. We conclude that benzalkonium chloride diluted in 70% alcohol (1:5,000) in vitro is the best disinfectant for human sclera after 24 h.

Bacillus cereus↗