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Decision strategies in the ABX (matching-to-sample) psychophysical task.

The ABX psychophysical task is very useful when the dimensions along which the stimulus varies cannot be readily described. However, there are a few problems associated with its use. First, the task has often been used with slight procedural variations, which hinders the extraction of a bias-free index of performance. Second, the most common index of sensitivity derived from this task is the ubiquitous proportion correct. Third, some researchers undervalue the importance that the decision strategy adopted by the observer has on estimates of sensitivity. We describe a standard methodology for the ABX task and outline models of performance based on two decision strategies. These models can be used to extract the detection-theoretic index of performance, d', from ABX data. The results of three ABX discrimination experiments, in which circle size, number value, and tone amplitude are used, are reported. Analysis of the decision spaces derived from observer responses indicates that these observers adopted a suboptimal difference decision strategy, even after they had received considerable practice with feedback. This suggests that, given no prior knowledge of the nature of the decision strategy adopted by an observer, the difference model of ABX discrimination could be a good choice when observer performance is estimated.

Adult↗

Nurses' personal pain experiences and their pain management knowledge.

BACKGROUND: This study examined nurses' personal experiences with pain and whether nurses' personal pain experiences were associated with initial pain management knowledge and ability to learn more about pain management. METHOD: This descriptive correlational study was a secondary analysis of a study that used a one-group pretest-posttest design to examine the effect of a pain management education program on nurses' pain management knowledge. The sample consisted of 177 nurses who answered yes to having had a personal pain experience requiring treatment. RESULTS: Nurses reported 13 types of pain, with pharmacological interventions comprising 94.4% of the pain treatments. Nurses' previous personal pain experiences were negatively related to their initial pain management knowledge (r = -.23, p < .01). CONCLUSION: Nurses' personal pain experience may impact their learning of pain management knowledge and should be considered when designing pain management education.

Adult↗

Assessing depression in community samples of children using self-report inventories: ethical considerations.

Although the methodological problems associated with the use of children's self-report depression inventories have previously been discussed in detail, the ethical problems related to the use of these instruments have been largely ignored. The primary purpose of this survey study was to determine how childhood depression researchers have prepared for and responded to children from community (i.e., nonclinic) samples whose nonanonymous scores on self-report depression inventories have indicated that they might be severely depressed or suicidal. Several recommendations, based upon the results of this survey study, the existing literature relevant to the ethical responsibilities of investigators who conduct research with children, and our own experiences with these instruments and populations, are made to assist researchers in their attempts to use these inventories in an ethical manner.

Adolescent↗

Morphological and neurotoxicological findings in tropical freshwater fish (Astyanax sp.) after waterborne and acute exposure to water soluble fraction (WSF) of crude oil.

The water-soluble fraction (WSF) of crude oil is a complex highly volatile and toxic mixture of hydrocarbon chains (polyaromatics, heterocyclics), phenols, and heterocyclic compounds containing nitrogen and sulfur. To evaluate the toxic effects of WSF in tropical freshwater teleosts and to develop methodologies that could investigate the toxic mechanisms of WSF in tropical organisms, an acute toxicity experiment was conducted with Astyanax sp. Three dilutions (15%, 33%, and 50%) of WSF obtained from Campos Bay's crude oil (Brazil) were used to study morphological and biochemical responses of the fish. Prior to exposure, the distribution and rate of volatilization of the WSF into each aquarium for the same exposure period was quantified by spectrofluorimetry. Five individuals of Astyvanax sp. were exposed to duplicate WSF of 0, 15, 33, and 50% for each of 12-, 24-, and 96-h exposures for a total of 120 individuals. Liver and gills were sampled from five fish from each treatment and were analyzed by histology, scanning and transmission electron microscopy. A fragment of muscle was also collected from each fish to measure acetylcholinesterase activity. Water analysis showed that only 4 h after dilution, an important loss of hydrocarbons in 33% and 50% of WSF was observed. In addition, 50% of hydrocarbon mass was lost in all tested dilutions after 24 h with significant difference for the 50% WSF at all measured times, demonstrating the high volatility of WSF in freshwater. Damage in the liver and the gills included the presence of necrosis, loss of hepatocytes limit, inflammation areas, cellular proliferation, aneurysms, and disorganization of the second lamellae. The 33% WSF significantly reduced acetylcholinesterase activity in fish. Our study demonstrated that the WSF of crude oil caused damage in organs and tissues of tropical freshwater Astyanax sp. and provided also the basis for a better understanding of the toxic mechanisms of WSF in freshwater fishes.

Acetylcholinesterase↗

[Influence of the sex of the physician in the alerting reaction in blood pressure determination in the elderly. EPICARDIAN Working Group].

BACKGROUND: Blood pressure measurements by a physician and to a lesser extent by a nurse often lead the patient to experience an alerting reaction associated with an increase in blood pressure. The main factor seems to be the interaction between the patient and the observer physicians, on average, record higher office blood pressure than nurses and non-physicians. There is no information about the influence of the physician's gender on alerting reaction. OBJECTIVE: To asses the influence of gender's doctor on alerting reaction in the elderly. METHODOLOGY: EPICARDIAN study is a cross-sectional survey of the civilian non-institutionalized elderly population in a health area, including an inhome interview (phase I, n = 1,200) and a clinical examination (phase II, a random sample n = 333). Three different blood pressure measurements were performed: conventional blood pressure was measured by trained nurses at the participant's home, and at the hospital by two physicians: a man (group A, n = 131, 46% men) and a women (group B, n = 152, 45% men). All subjects were informed by a nurse that they were going to be interviewed by a doctor. Twenty-four hour ambulatory blood pressure was recorded at 15 minute intervals, awake and sleep periods were defined based on daily activities. Alerting reaction was defined as the difference between means at home, office and awake period. RESULTS: Office blood pressures were distinctively higher than the awake average (15 mmHg for systolic blood pressure and 7 mmHg for diastolic respectively) in 83% of subjects. Differences between clinical and awake average blood pressure were significantly higher in females than in males (17 +/- 16 and 12 +/- 16 respectively, p < 0.0001) and increased, in both sexes with increasing age (p = 0.001) and clinic blood pressure values (p < 0.001). There were no sex, age, body mass index, casual home blood pressure and awake blood pressure period differences between groups A and B. Group A, interviewed by a man, showed an average of 10 mmHg more than group B, interviewed by a woman, on office alerting reaction (22 +/- 18/11 +/- 11 mmHg versus 12 +/- 16/3.6 +/- 10 mmHg systolic blood pressure/diastolic blood pressure alerting reaction, respectively p < 0.001 in both cases). Differences were similar when analizying men and women independently. CONCLUSION: Alerting reaction during blood pressure measurement in the elderly is higher when the observer is a male physician than when the physician is a female, independent of the patient's gender.

Aged↗

Family caregiving to those with dementia in rural Alabama: racial similarities and differences.

This study explored differences and similarities in the experiences of African American and White family caregivers of dementia patients living in rural Alabama. This cross-sectional survey used a caregiving stress model to investigate the interrelationships between caregiving burden, mediators, and outcomes. Random-digit-dialing telephone interviews were used to obtain data on a probability sample of 74 non-Hispanic White and 67 African American caregivers. White caregivers were more likely to be married and older, used acceptance and humor as coping styles, and had fewer financial problems. African American caregivers gave more hours of care, used religion and denial as coping styles, and were less burdened. The authors have developed a methodology for obtaining a representative sample of African American and White rural caregivers. Further investigations are needed of the interactions between urban/rural location and ethnic/racial backgrounds of dementia caregivers for heuristic and applied reasons.

Adaptation, Psychological↗

Genetic structure of an introduced pest, grape phylloxera (Daktulosphaira vitifoliae Fitch), in Europe.

A model for the genetic structure of grape phylloxera populations in Europe was developed using hierarchical sampling techniques and AFLP-PCR (amplified fragment length polymorphism--polymerase chain reaction) methodology. One-hundred three European and 6 North American phylloxera populations were studied. An additional European sampling set comprising 60 samples was analyzed to study regional subdivision. The populations grouped into two clusters loosely correlated with collection site location. Phylloxera populations collected from northern (above lat 43 degrees) geographic regions were significantly different from southern (below 43 degrees) populations. The northern cluster was more heterogeneous than the southern cluster, possibly reflecting holocyclic versus anholocyclic reproduction. Microgeographic scales of phylloxera genetic structure displayed as much variation within as among host plants. The host plant did not affect the genetic structure of European phylloxera as revealed in two independent experiments.

Animals↗

The oral health status in Mahonisi: a community with very low levels of dental caries.

The oral health status and treatment needs of the community at Mahonisi were determined in order to plan a health care programme. The caries experience, occlusal status, fluorosis prevalence and prosthetic status and needs were determined according to the WHO pathfinder methodology (1987). The dmft for the 4- 5- and 6-year-old children was 0.33 and 0.67 respectively. The DMFT ranged from 0 in the 12- and 15-year-old children to 4.2 in the 65-years-and-older age group. Among the 12-, 15- and 18-year-old pupils 73% had good occlusions, 18% had slight and 9% more serious anomalies. Of the 152 subjects examined for fluorosis only 2 exhibited very mild dental fluorosis. No soft-tissue lesions were seen in the adult sample, but 10 cases were seen in the schoolchildren. No edentulous person was seen, but 4 people needed extraction of all their remaining teeth. A need for 7 partial dentures was recorded. The simplified oral hygiene index (OHI-S) ranged from 1.45 in the 4- 5-year-old children to 4.47 in the oldest group. The zero DMFT scores for the 12- and 15-year-old children are exceptional and need to be further investigated. It is recommended that the teachers in the pre-school and some of the primary school be trained to provide oral hygiene instruction to the children.

Adolescent↗

Simulations of flow-induced director structures in nematic liquid crystals through leslie-ericksen equations. I. Computational methodology in two dimensions

A computational treatment of the constitutive equations of nematodynamics, based on the Leslie-Ericksen approach, is presented and discussed for a rotating planar nematic sample subjected to a constant magnetic field. The dynamics of the velocity v and director n fields is taken into account exactly. Coupled partial differential equations suitable to be solved numerically are worked out, in terms of derived functionals of v and n and of their spatial and time derivatives. Time-dependent patterns of the director are obtained using a finite-difference scheme in a spatial polar grid. Several experimental situations are analyzed, corresponding to common experimental setups: continuously rotating samples for different values of the rotational speed; 30 degrees and 90 degrees step-rotation experiments. A comparison is made to existing approximate treatments. Dependence upon the sample dimension is also discussed.

Journal Article↗

A regulatory view on adaptive/flexible clinical trial design.

Recently there is growing interest in use of adaptive or flexible designs for development of pharmaceutical products. Statistical methodology has been greatly advanced in the literature. However, there are still some important issues with the methodology and application. In addition, there are many other challenges with these designs, including efficiency of these designs in the entire development program, trial conduct and logistics, the infrastructure of an adaptive trial, the regulatory evaluation of trial results and trial conduct, etc. Up till now, regulatory experience in these designs is very limited. We share some of the challenges.

Biometry↗

'You do know he's had a stroke, don't you?' Preparation for family care-giving--the neglected dimension.

Countries throughout the developed world have introduced a policy of community care for older people to reduce costs to the state and maintain quality of life. In reality community care is largely family care and recognition of the need to support family carers is being promoted through the notion of partnership with professional carers. Such a partnership calls for a more complete understanding of how carers' needs change over time and how professional support can be most effective. Support is particularly important at the start of care-giving in order that carers can exercise free choice and be adequately prepared for their role. This paper provides an overview an ongoing longitudinal study and reports specifically on the findings of data from a preliminary study in which a convenience sample of seven experienced carers of stroke survivors who attended a stroke and carers club were interviewed in their own homes. Based on initial data from a longitudinal study of stroke victims, this paper outlines four themes: 'What's it all about', 'Going it alone', 'Up to the job' and 'What about me?' These themes highlight the difficulties carers experience in the immediate aftermath of stroke.

Caregivers↗

Does vasectomy cause testicular cancer?

Between 1975 and 1990, 330 men developed testicular tumours in Northern Ireland. Their names were cross matched with a sample of 2904 men who had undergone vasectomy between 1970 and 1985. The expected numbers of tumours in vasectomised patients was 2, while the actual number was 1. Our experience does not indicate that vasectomy causes testicular cancer.

Adult↗

Pregnancy rates among U.S. women and their partners in 1994.

CONTEXT: When rates of pregnancy, birth and abortion are calculated only for the women involved, men's role in reproduction is ignored, resulting in limited understanding of their influence on these outcomes. METHODS: Data from the 1995 National Survey of Family Growth and from the 1994-1995 Alan Guttmacher Institute Abortion Patient Survey were combined with national natality statistics to estimate pregnancy rates in 1994 for women and their male partners, by age and marital status at the time of conception. RESULTS: Nine percent of both men and women aged 15-44 were involved in conceiving a pregnancy in 1994 (excluding those resulting in miscarriages). Pregnancy levels were highest among women aged 20-24 and among male partners aged 25-29. Men younger than 20 were involved in about half as many pregnancies as were women this age (9% compared with 18%). In contrast, men aged 35 and older were involved in roughly twice as many pregnancies as were similarly aged women (19% compared with 9%). Three out of every four pregnancies in 1994 resulted in a birth. However, 47% of pregnancies involving men younger than 18 ended in abortion, compared with about 34% of those involving men aged 40 and older. In comparison, 31% of pregnancies among women younger than 18 resulted in abortion, while 39% of those among women aged 40 and older were terminated. CONCLUSION: The overall rate at which men were involved in causing a pregnancy is similar to the pregnancy rate among women. Men are typically older than women when they are involved in a pregnancy, however. This implies that men may bring more experience and resources to the pregnancy experience.

Abortion, Induced↗

Menstrual and premenstrual experiences of women in a developing country.

The menstrual and premenstrual experiences of black Zimbabwean women were examined. Twenty-five professional women and twenty-five domestic workers were interviewed using semistructured, open-ended interviews designed to explore their experiences. An analysis of consensual data indicated a number of predominant themes, including secrecy associated with menstruation, the negativity surrounding menarche, the breakdown of the traditional family network that passes on information about menstruation, and the acceptance of menstrual cycle experiences by the women. The main differences between the two groups concerned explanations of the functions and purpose of menstruation and the reporting of physical and affective symptoms. These differences are suggestive of the impact of educational level on experiences of menstruation.

Adult↗

How does spatial extent of fMRI datasets affect independent component analysis decomposition?

Spatial independent component analysis (sICA) of functional magnetic resonance imaging (fMRI) time series can generate meaningful activation maps and associated descriptive signals, which are useful to evaluate datasets of the entire brain or selected portions of it. Besides computational implications, variations in the input dataset combined with the multivariate nature of ICA may lead to different spatial or temporal readouts of brain activation phenomena. By reducing and increasing a volume of interest (VOI), we applied sICA to different datasets from real activation experiments with multislice acquisition and single or multiple sensory-motor task-induced blood oxygenation level-dependent (BOLD) signal sources with different spatial and temporal structure. Using receiver operating characteristics (ROC) methodology for accuracy evaluation and multiple regression analysis as benchmark, we compared sICA decompositions of reduced and increased VOI fMRI time-series containing auditory, motor and hemifield visual activation occurring separately or simultaneously in time. Both approaches yielded valid results; however, the results of the increased VOI approach were spatially more accurate compared to the results of the decreased VOI approach. This is consistent with the capability of sICA to take advantage of extended samples of statistical observations and suggests that sICA is more powerful with extended rather than reduced VOI datasets to delineate brain activity.

Acoustic Stimulation↗

Communicative abilities in autism: evidence for attentional deficits.

Although there are many theories about autism, something all of them agree upon is that autistics are impaired in the ability to communicate. The explanation is either their incapacity to attribute mental states to others or the interference of irrelevant stimuli with the access and processing of the communication (low). Our study on mute autistic children aims to investigate their communicative ability in order to bring some new evidence on the debate. We used an experimental technique that allows autistic children to access and process the communicative acts in a familiar context for as long as needed. The experimental results show that our sample of autistic children performs as well as the control group of normal children in dealing with directs, indirects, ironies, deceits, and recoveries of failure. Independent of their respective difficulty, the felicitous outcome of any of these acts requires the capacity to attribute an adequate communicative intention to the actor. Moreover, our results show that, contrary to the established findings in the literature, autistics' performance in the standard false belief task, a task that requires one to understand the mental states of other people, is equivalent to the performance of normal subjects. We argue that an attentional deficit affects the communicative performance of autistics in experiments where classic methodologies are used; with the proper methodology, we can access the unexplored world where mute autistic children also communicate. As far as we know, this is the first systematic experiment on pragmatic abilities in mute autistic children. Indeed, our work shows that tests and methodologies which help to focus on the communicative task improve the autistics' performance with respect to those used in the literature. We conclude that the autistic communicative deficit is at the performance level and that it has an attentional nature.

Adolescent↗

Detection of explosives and their degradation products in soil environments.

Polynitro organic explosives [hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX), octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine (HMX) and 2,4,6-trinitrotoluene (TNT)] are typical labile environmental pollutants that can biotransform with soil indigenous microorganisms, photodegrade by sunlight and migrate through subsurface soil to cause groundwater contamination. To be able to determine the type and concentration of explosives and their (bio)transformation products in different soil environments, a comprehensive analytical methodology of sample preparation, separation and detection is thus required. The present paper describes the use of supercritical carbon dioxide (SC-CO2), acetonitrile (MeCN) (US Environmental Protection Agency Method 8330) and solid-phase microextraction (SPME) for the extraction of explosives and their degradation products from various water, soil and plant tissue samples for subsequent analysis by either HPLC-UV, capillary electrophoresis (CE-UV) or GC-MS. Contaminated surface and subsurface soil and groundwater were collected from either a TNT manufacturing facility or an anti-tank firing range. Plant tissue samples were taken fromplants grown in anti-tank firing range soil in a greenhouse experiment. All tested soil and groundwater samples from the former TNT manufacturing plant were found to contain TNT and some of its amino reduced and partially denitrated products. Their concentrations as determined by SPME-GC-MS and LC-UV depended on the location of sampling at the site. In the case of plant tissues, SC-CO2 extraction followed by CE-UV analysis showed only the presence of HMX. The concentrations of HMX (<200 mg/kg) as determined by supercritical fluid extraction (SC-CO2)-CE-UV were comparable to those obtained by MeCN extraction, although the latter technique was found to be more efficient at higher concentrations (>300 mg/kg). Modifiers such as MeCN and water enhanced the SC-CO2 extractability of HMX from plant tissues.

Azocines↗

Effect of chemical composition of sunflower seed meal on its true metabolizable energy and amino acid digestibility.

The effect of chemical composition of sunflower seed meal (SFSM) on TMEn and true amino acid digestibility (TAAD) was studied. In Experiment 1, the excretion pattern of three SFSM samples force-fed (30 g) to 10 adult cockerels (Hy-Line) each was followed for 84 h to determine the time interval for complete excretion of SFSM. Type of SFSM did not affect the excretion pattern of DM and energy (P=0.438, and P=0.189, respectively). Dry matter and energy excreted every 12 h decreased linear and quadratically (P < 0.001) with collection time. No differences were found from 48 h collection time on. So, an excreta collection period of 48 h was considered adequate for determining the TMEn of SFSM. In Experiment 2, 135 adult cockerels were force-fed to determine the TMEn of 11 samples of SFSM. Type of SFSM affected TMEn (P < 0.001), which ranged from 1,558 to 2,023 kcal/kg DM for SFSM of 31 to 42% CP, respectively. The TMEn was highly correlated (P < 0.001) to hemicellulose (r=-0.90), acid detergent lignin (r=-0.84), neutral detergent fiber (r=-0.82), and CP (r=0.77). Four prediction equations are proposed, the most practical being: TMEn (kcal/kg DM)=2,816.8 - 109.5 hemicellulose (%DM), RSD=70.2. Three out of the 11 samples of SFSM were selected for determining TAAD and the effect of endogenous amino acid correction. The methodology used was that of the TMEn assay, but one more estimation of amino acid endogenous excretion was made using a N-free diet with 85% cornstarch and 15% cellulose. Endogenous amino acid excretion was greater for roosters fed the N-free diet than those deprived of feed, resulting in a higher digestibility (from 0.7 to 2.7%, P < 0.05) only for six amino acids: threonine, valine, alanine, proline, and aspartic and glutamic acids. No interaction was detected (P=0.94) between type of SFSM and method of estimation of endogenous amino acid excretion. The TAAD of SFSM increased significantly (P < 0.001) with the CP content, total TAAD being 86, 88, and 89% for SFSM of 32, 35, and 37% CP, respectively. Attention should be paid when including high fiber-low protein SFSM in poultry diets to balance its lesser digestible amino acids contribution, mainly in lysine (from 0.77 to 1.06% for SFSM of 32 and 37% CP, respectively).

Amino Acids↗